scholarly journals Limb perfusion in the lower limb amputee—a comparative study using a laser Doppler flowmeter and a transcutaneous oxygen electrode

1987 ◽  
Vol 11 (2) ◽  
pp. 80-84 ◽  
Author(s):  
S. L. E. Fairs ◽  
R. O. Ham ◽  
B. A. Conway ◽  
V. C. Roberts
Keyword(s):  
Laser Doppler Flowmetry ◽  
Lower Limb ◽  
Local Heating ◽  
Objective Assessment ◽  
Relative Increase ◽  
Laser Doppler ◽  
Transcutaneous Oxygen ◽  
Doppler Flowmetry ◽  
Amputation Level ◽  
Significant Difference

Accurate and objective assessment of amputation level in the lower limb plays an important role in patient management. Laser Doppler flowmetry (LDF) is a new and noninvasive technique for skin blood flow measurement and has been used pre-operatively in 25 patients undergoing amputation for vascular disease and in five normal controls. Baseline flux measurements were made at room temperature on the medial aspect of legs and then again after local heating of the skin for five minutes. Transcutaneous oxygen measurements were made at the same site for comparison and amputation level in patients selected on this basis. Significant differences (p<0.001) in TcPO2 values were found between controls (10.9±0.5kPa), below-knee (BK) amputees (6.0±1.5kPa) and above-knee (AK) amputees (1.5±0.6kPa). Baseline LDF flux did not differ significantly between any group. Heated flux values did however show a significant difference (p<0.005) between controls (52.4±23.5) and both BK (20.6±9.2) and AK groups (8.1±7.7) and also between the amputee groups. The relative increase in flux (heated flux/baseline flux) differed significantly between the BK (3.3±1.5) and AK (1.2±0.3) groups (p<0.001) and between these two and the controls (11.2±5.4) (p<0.001). The correlation between relative increase in flux and TcPO2 was 0.7 (p<0.001). It is concluded that laser Doppler flowmetry used in conjunction with thermal stressing could provide a quick, simple and non-invasive method for objectively determining amputation level in the lower limb.

scholarly journals Increasing the diagnostic signifcance of the laser Doppler flowmetry in assessing skin microcirculation in hypertension

2019 ◽  
Vol 25 (1) ◽  
pp. 74-83 ◽  
Author(s):  
P. A. Glazkova ◽  
S. A. Terpigorev ◽  
D. A. Kulikov ◽  
N. A. Ivanova ◽  
A. A. Glazkov
Keyword(s):  
Healthy Volunteers ◽  
Laser Doppler Flowmetry ◽  
Local Heating ◽  
Relative Increase ◽  
Laser Doppler ◽  
Instrumental Method ◽  
Skin Microcirculation ◽  
Doppler Flowmetry ◽  
Forearm Skin ◽  
Heating Test

Background.Hypertension (HTN) is associated with impaired skin microcirculation. Laser Doppler flowmetry is an objective, quantitative, instrumental method that allows evaluating skin microcirculation. However, the method was not widely used clinically due to high variability of perfusion and small difference between healthy people and HTN patients and, as a consequence, low diagnostic signifcance.Objective.To provide the grounds for the approaches increasing the informative value of skin microcirculation measurement by laser Doppler flowmetry in HTN patients.Design and methods.The study involved HTN patients (n = 13, the median age was 60 (49; 63) years) and young otherwise healthy volunteers without HTN (n = 12, the median age 26 (25; 27) years). Microcirculation measurement was performed by laser Doppler flowmetry using LAKK-02 device. Registration of microcirculation on the forearm skin was carried out during the occlusionheating test. The Mann-Whitney test was used to compare the parameters in two groups. The diagnostic accuracy of the method for the inverse classifcation of the subjects was evaluated using ROC analysis.Results.In HTN patients, the median baseline perfusion was 3,1 (1,84; 4,31) perfusion units (PU), in healthy volunteers — 4,29 (3,66; 8,14) PU (p = 0,04). The median area under the microcirculation curve for the frst 2 minutes of heating in HTN patients was 1206,7 (813; 1449) PU × s, in healthy volunteers — 1552,3 (1310; 1624) PU × s (p = 0,035). In healthy volunteers, the heating increased the perfusion by 596 % (386%; 878%), and in HTN patients perfusion increased only by 265% (180 %; 318%) (p = 0,01). The relative increase in perfusion during postocclusion hyperemia with continued heating compared with the baseline in healthy volunteers was 651% (493 %; 999%), and in HTN patients — 302 % (182 %; 436%) (p = 0,005). Thus, when comparing the average parameters for each period in the occlusion-heating test, only basic perfusion showed signifcant differences. However, when changed from absolute to relative parameters (the increase in microcirculation in relation to the vasodilating effects), the difference was signifcant. Moreover, sensitivity achieved was 75 % and specifcity — 84,6% (the inverse classifcation of groups).Conclusions.The physiological (the local heating of the forearm skin at a rate of 2 degrees Celsius per second, a combination of vasodilating effects) and mathematical (the transition from absolute to relative values) approaches provided an increase of the informative value of the laser Doppler flowmetry, as well as its sensitivity and specifcity.

scholarly journals Evaluation of the marginal gingival health using laser doppler flowmetry

2006 ◽  
Vol 17 (3) ◽  
pp. 219-222 ◽  
Author(s):  
Hakan Develioglu ◽  
Bülent Kesim ◽  
Aykut Tuncel
Keyword(s):  
Blood Flow ◽  
Laser Doppler Flowmetry ◽  
Laser Doppler ◽  
Plaque Index ◽  
Tissue Blood Flow ◽  
Doppler Flowmetry ◽  
Gingival Health ◽  
Significant Difference ◽  
Clinical Indices ◽  
And Control

The purposes of this study were to compare the gingival blood flow (GBF) in test sites (teeth retaining fixed partial dentures) and control sites (contralateral natural teeth) and investigate whether there is any relationship between clinical indices and GBF values. Twelve healthy subjects (6 females and 6 males) aged 20 to 54 years were enrolled this study. The GBF was measured from the middle point of the marginal gingiva in the test and control sites using laser Doppler flowmetry (LDF). Additionally, plaque index, gingival index and probing depth measurements were recorded. Statistically significant difference (p<0.05) was found between the test and control sites for marginal GBF. In contrast, no significant difference (p>0.05) was found between test and control sites with respect to the clinical indices, except for plaque index. The findings of this study suggest that there is a significant relation between resin-bonded fixed partial dentures with margins located subgingivally and marginal GBF. Clinical indices are helpful to collect information about the clinical health status of gingival tissues, but GBF is a good tool to measure gingival tissue blood flow and assess periodontal health. In conclusion, laser Doppler flowmetry can be used together with clinical indices to evaluate the marginal gingival health.

The acute impact of local cooling versus local heating on human skin microcirculation using laser Doppler flowmetry and tissue spectrophotometry

Burns ◽  
2020 ◽  
Vol 46 (1) ◽  
pp. 104-109 ◽  
Author(s):  
Dominik Bender ◽  
Stephanie Tweer ◽  
Frank Werdin ◽  
Jens Rothenberger ◽  
Adrien Daigeler ◽  
...  
Keyword(s):  
Human Skin ◽  
Laser Doppler Flowmetry ◽  
Local Heating ◽  
Laser Doppler ◽  
Local Cooling ◽  
Skin Microcirculation ◽  
Doppler Flowmetry

Vascular Assessment in Patients With a Lower Limb Wound: A Correlational Study of Photoplethysmography and Laser Doppler Flowmetry Toe Pressure Techniques

2020 ◽  
pp. 193229682097997
Author(s):  
Jérôme Patry ◽  
Louis Laurencelle ◽  
Justine Bélisle ◽  
Maryse Beaumier
Keyword(s):  
Laser Doppler Flowmetry ◽  
Pressure Measurement ◽  
Lower Limb ◽  
Laser Doppler ◽  
Lower Limbs ◽  
Correlational Study ◽  
Care Clinic ◽  
Doppler Flowmetry ◽  
Vascular Assessment ◽  
Toe Pressure

Background: Vascular assessment of the lower limbs is essential in patients with diabetes. In the presence of noncompressible arteries, the ankle brachial index (ABI) can either be inconclusive or provide false-positive results. Toe pressure measurement has been suggested as an alternative as a noninvasive method for detecting peripheral arterial disease (PAD). Toe pressure measurement can be performed either by photoplethysmography (PPG) or by Laser Doppler flowmetry (LDF). The aim of this study was to determine correlations between the two techniques in order to promote the use of PPG in clinical practice. Methods: This was a prospective correlational study of 108 consecutive recruited adult patients, with and without diabetes, with at least one lower limb wound from a University-affiliated hospital wound care clinic. Toe pressure measurements were both performed with PPG and LDF devices. Results: Mean toe pressure values for PPG and LDF were, respectively, 83.7 (SD 35.4) and 79.5 (SD 32.0) mmHg (with a paired t-test 3.969, P < 0.01). In patients with at least one lower limb wound, a strong linear relation was found between PPG and LDF toe pressure techniques with a Pearson’s r correlation coefficient of 0.920 ( P < 0.001). Conclusions: PPG and LDF toe pressure techniques are equivalent in patients with at least one lower limb wound, irrespective of the presence of diabetes. Therefore, in the presence of an ABI with inconclusive results, such as in a patient with noncompressible vessels, both toe pressure techniques can be used for assessing the vascular supply of the lower limb with a wound.

Evaluation of Pulpal Blood Flow Changes in Primary Molars with Physiological Root Resorption by Laser Doppler Flowmetry and Pulse Oximetry

2011 ◽  
Vol 36 (2) ◽  
pp. 139-144 ◽  
Author(s):  
Hüseyin Karayilmaz ◽  
Zuhal Kirzioğlu
Keyword(s):  
Blood Flow ◽  
Pulse Oximetry ◽  
Laser Doppler Flowmetry ◽  
Root Resorption ◽  
Laser Doppler ◽  
Primary Molars ◽  
Primary Molar ◽  
Doppler Flowmetry ◽  
Pulpal Blood Flow ◽  
Significant Difference

Aim: The aim of this study was to undertake a comprehensive quantitative investigation of pulpal blood flow (PBF) changes in human non-carious primary molar teeth with variable degrees of root resorption by Laser Doppler Flowmetry (LDF) and Pulse Oximetry (PO) methods. Materials and Methods: Data was collected from clinically and radiographically healthy 86 mandibular primary molars which have different physiological root resorption levels (PRRLs). PRRLs for each of the teeth were assessed using periapical radiographs and teeth were subdivided into three groups. Results: The LDF values demonstrated a significant difference (p = 0.0001) between all groups although PO did not demonstrate any difference (p = 0.109). Statistical analysis of LDF values demonstrated significant differences between Groups A and C (p = 0.0001) and Groups B and C (p = 0.008). Furthermore, positive correlations were determined between LDF values and PRRL groups (p = 0.0001) and patients' ages (p = 0.0001). Conclusions: In our study, it was observed that the PBF values of human primary molars measured by LDF tended to increase with the progress of physiological root resorption and age. LDF was found to be a more effective method than PO to assess the pulpal vascularity changes of human primary molars.

scholarly journals Difference in Moxibustion-Induced Microcirculatory Responses between the Heart and Lung Meridians Assessed by Laser Doppler Flowmetry

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Yongliang Jiang ◽  
Hantong Hu ◽  
Xiaoyu Li ◽  
Jiali Lou ◽  
Yajun Zhang ◽  
...  
Keyword(s):  
Laser Doppler Flowmetry ◽  
Body Surface ◽  
Intervention Group ◽  
The Body ◽  
Laser Doppler ◽  
Doppler Flowmetry ◽  
Heart Meridian ◽  
Significant Difference ◽  
Lung Meridian ◽  
Distal Site

Objective. By comparing the differences in microcirculatory responses of the heart and lung meridians induced by moxibustion on these two meridians, respectively, this study aimed to investigate the specificity for site-to-site association on body surface between different meridians. Methods. Eighty healthy adults were enrolled and divided into the lung meridian intervention group and heart meridian intervention group in a ratio of 1 : 1. Three-channel laser Doppler flowmetry was used to monitor microcirculatory responses for the heart and lung meridians. Primary outcome was change of blood perfusion units (PU) of three measurement sites along the two meridians. Results. In the lung meridian intervention group, following moxibustion performed at LU5 of the lung meridian, PU in the distal site of the lung meridian increased significantly. By contrast, the PU of HT3 in the heart meridian, which was nearest to the moxibustion site, did not change significantly. PU in the distal site of the heart meridian declined. Meanwhile, significant difference in PU change was detected between the distal site of the lung meridian and the other two control sites of the heart meridians during moxibustion and postmoxibustion. Alternatively, similar to the results of the lung meridian intervention group, the specificity of microcirculatory response between the heart and lung meridians was observed in the heart meridian intervention group. Conclusions. For the heart and lung meridians, the effect of moxibustion-induced microcirculatory response may be more related to meridian routes than the specific distance between two sites located at different meridians, thereby supporting possible specificity for site-to-site association on the body surface between these two meridians. Nevertheless, given that only two meridians and limited measurement sites were compared, all current findings are not sufficiently robust. Further research should be conducted to investigate more meridians and measurement sites.

Relative Importance of Flow versus  Pressure in Splanchnic Perfusion during Cardiopulmonary Bypass in Rabbits

2000 ◽  
Vol 92 (2) ◽  
pp. 457-457 ◽  
Author(s):  
Olivier Bastien ◽  
Vincent Piriou ◽  
Abdellah Aouifi ◽  
Claire Flamens ◽  
Rhys Evans ◽  
...  
Keyword(s):  
Blood Flow ◽  
Cardiopulmonary Bypass ◽  
Laser Doppler Flowmetry ◽  
Laser Doppler ◽  
Analysis Of Covariance ◽  
Tissue Blood Flow ◽  
Splanchnic Perfusion ◽  
Doppler Flowmetry ◽  
Significant Difference ◽  
Splanchnic Ischemia

Background Decreased gastrointestinal perfusion has been reported during cardiopulmonary bypass (CPB). Conflicting results have been published concerning thresholds of pressure and flow to avoid splanchnic ischemia during CPB. This study compared splanchnic perfusion during independent and randomized variations of CPB pump flow or arterial pressure. Methods Ten rabbits were studied during mild hypothermic (36 degrees C) nonpulsatile CPB using neonatal oxygenators. Simultaneous measurements of tissue blood flow in four different splanchnic areas (gastric, jejunum, ileum, and liver) were performed by laser Doppler flowmetry (LDF) before CPB (T0) and during a 4-step factorial experimental block design. Pressure and flow were alternatively high or low in random order. Results Laser Doppler flowmetry was significantly lower than pre-CPB value but was better preserved (analysis of covariance) in all organs, except liver, when CPB flow was high, whatever the pressure. Splanchnic LDF values in the low- versus high-flow groups expressed as perfusion unit were (mean +/- SD): stomach, 94+/-66 versus 137+/-75; jejunum, 118+/-78 versus 172+/-75; ileum, 95+/-72 versus 146+/-83; and liver, 79+/-72 versus 108+/-118. No significant difference of LDF was observed between the high- and low-pressure groups, whatever the flow, except for liver: stomach, 115+/-64 versus 117+/-83; jejunum, 141+/-80 versus 148+/-83; ileum, 127+/-87 versus 114+/-76; liver, 114+/-88 versus 73+/-70. Conclusion Prevention of splanchnic ischemia during CPB should focus on preservation of high CPB blood flow rather than on high pressure.

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