Immunohistochemical localization of interferon-γ in normal human ovary

1994 ◽  
Vol 8 (3) ◽  
pp. 161-168 ◽  
Author(s):  
G. Grasso ◽  
A. Asano ◽  
T. Minagawa ◽  
T. Tanaka ◽  
S. Fujimoto ◽  
...  
Keyword(s):  
Interferon Γ ◽  
Immunohistochemical Localization ◽  
Human Ovary ◽  
Normal Human

scholarly journals Localization of Indoleamine 2,3-Dioxygenase-1 and Indoleamine 2,3-Dioxygenase-2 at the Human Maternal-Fetal Interface

2020 ◽  
Vol 13 ◽  
pp. 117864692098416
Author(s):  
Yoshiki Kudo ◽  
Iemasa Koh ◽  
Jun Sugimoto
Keyword(s):  
Interferon Γ ◽  
Kynurenine Pathway ◽  
Explant Culture ◽  
Immunohistochemical Localization ◽  
Tryptophan Metabolism ◽  
Indoleamine 2,3 Dioxygenase ◽  
Cellular Expression ◽  
Normal Human ◽  
Protein Immunoreactivity ◽  
Maternal Fetal Interface

Immunohistochemical localization of indoleamine 2,3-dioxygenase-1 and indoleamine 2,3-dioxygenase-2, the first and rate-limiting enzyme in tryptophan metabolism along the kynurenine pathway, has been studied in order to better understand the physiological significance of these enzymes at the maternal-fetal interface of human pregnancy with a gestational age of 7 weeks (n = 1) and term placentas (37-40 weeks of gestation, n = 5). Indoleamine 2,3-dioxygenase-1 protein immunoreactivity was found in glandular epithelium of the decidua and the endothelium of the fetal blood vessels in the villous stroma with some additional positive cells in the villous core and in the decidua. The syncytiotrophoblast stained strongly for indoleamine 2,3-dioxygenase-2. Immunoreactivity of kynurenine, the immediate downstream product of indoleamine 2,3-dioxygenase-mediated tryptophan metabolism, showed the same localization as that of indoleamine 2,3-dioxygenase-1 and indoleamine 2,3-dioxygenase-2, suggesting these are functional enzymes. Interferon-γ added to placental villous explant culture markedly stimulated expression level of both mRNA and immunoreactivity of indoleamine 2,3-dioxygenase-1. The different cellular expression and interferon-γ sensitivity of these enzymes at the maternal-fetal interface suggests distinct physiological roles for each enzyme in normal human viviparity.

Immunohistochemical localization of 17α-hydroxylase/C17–20 lyase and aromatase cytochrome P-450 in the human ovary during the menstrual cycle

1992 ◽  
Vol 135 (3) ◽  
pp. 589-NP ◽  
Author(s):  
T. Tamura ◽  
J. Kitawaki ◽  
T. Yamamoto ◽  
Y. Osawa ◽  
S. Kominami ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Corpus Luteum ◽  
Granulosa Cells ◽  
Polyclonal Antibodies ◽  
Immunohistochemical Localization ◽  
Corpora Lutea ◽  
Human Ovary ◽  
Normal Human ◽  
Theca Interna ◽  
Cytochrome P 450

ABSTRACT Immunohistochemical localization of 17α-hydroxylase/C17–20 lyase (P-45017α,lyase) and aromatase cytochrome P-450 (P-450arom) in normal human ovaries during the menstrual cycle was studied using specific polyclonal antibodies which were raised against corresponding enzymes. In the follicular phase of matured follicles, P-45017α,lyase was localized in theca interna cells and P-450arom in granulosa cells. P-45017α,lyase was expressed in theca interna cells before P-450arom was expressed in granulosa cells. The corpus luteum showed immunoreactivity to both enzymes and, after menstruation, immunoreactivity decreased gradually until it could not be detected in the corpus albicans. In corpus luteum graviditatis the immunoreactivity continued to be expressed strongly. In some atretic follicles, P-45017α,lyase and/or P-450arom continued to be expressed. In the stromal layer, P-45017α,lyase was detected in secondary interstitial cells, which originated from the theca interna of atretic follicles, and P-450arom was detected in hilar cells. Immunoreactivity to both enzymes was also detected in oocytes of developing follicles. These results are consistent with the two cell theory in the human ovary. They also suggest that androgens and oestrogens are produced not only by follicles and corpora lutea but also by stroma and oocytes. Journal of Endocrinology (1992) 135, 589–595

Immunohistochemical localization of pituitary gonadotrophins and gonadal steroids confirms the 'two-cell, two-gonadotrophin' hypothesis of steroidogenesis in the human ovary

1990 ◽  
Vol 126 (3) ◽  
pp. 483-NP ◽  
Author(s):  
M. Kobayashi ◽  
R. Nakano ◽  
A. Ooshima
Keyword(s):  
Luteal Phase ◽  
Immunohistochemical Analysis ◽  
Immunohistochemical Localization ◽  
Follicular Phase ◽  
Gonadal Steroids ◽  
Immunohistochemical Method ◽  
Human Ovary ◽  
Luteal Cells ◽  
Menstrual Cycles

ABSTRACT Ovaries from 37 women with normal menstrual cycles were analysed for localization of pituitary gonadotrophins and gonadal steroids using an immunohistochemical method. In the follicular phase, FSH and oestradiol-17β localized in the granulosa layer, and LH, progesterone and testosterone localized in the internal thecal layer. In the luteal phase, gonadotrophins and steroids localized in luteal cells. Particularly in the early luteal phase, FSH and oestradiol-17β localized in large luteal cells, and LH, progesterone and testosterone localized in small luteal cells. The results of the present immunohistochemical analysis confirm the two-cell, two-gonadotrophin hypothesis of steroidogenesis in the human ovary. Journal of Endocrinology (1990) 126, 483–488

scholarly journals Immunohistochemical localization of periostin in human gingiva

2015 ◽  
Vol 59 (3) ◽  
Author(s):  
T. Cobo ◽  
A. Obaya ◽  
S. Cal ◽  
L. Solares ◽  
R. Cabo ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Western Blot ◽  
Protein Band ◽  
Immunohistochemical Localization ◽  
Matricellular Protein ◽  
Double Immunofluorescence ◽  
Periodontal Tissues ◽  
Human Gingiva ◽  
Normal Human ◽  
Bidirectional Interactions

<p>The periostin is a matricellular protein expressed in collagen-rich tissues including some dental and periodontal tissues where it is regulated by mechanical forces, growth factors and cytokines. Interestingly the expression of this protein has been found modified in different gingival pathologies although the expression of periostin in normal human gingiva was never investigated. Here we used Western blot and double immunofluorescence coupled to laser-confocal microscopy to investigated the occurrence and distribution of periostin in different segments of the human gingival in healthy subjects. By Western blot a protein band with an estimated molecular mass of 94 kDa was observed. Periostin was localized at the epithelial-connective tissue junction, or among the fibers of the periodontal ligament, and never co-localized with cytokeratin or vimentin thus suggesting it is an extracellular protein. These results demonstrate the occurrence of periostin in adult human gingiva; its localization suggests a role in the bidirectional interactions between the connective tissue and the epithelial cells, and therefore in the physiopathological conditions in which these interactions are altered.  </p>

Immunohistochemical localization and distribution of torsinA in normal human and rat brain

2000 ◽  
Vol 853 (2) ◽  
pp. 197-206 ◽  
Author(s):  
P. Shashidharan ◽  
Brian C. Kramer ◽  
Ruth H. Walker ◽  
C.Warren Olanow ◽  
Mitchell F. Brin
Keyword(s):  
Rat Brain ◽  
Immunohistochemical Localization ◽  
Normal Human

Expression of inhibin α,βA and βB messenger ribonucleic acids in the normal human ovary and in polycystic ovarian syndrome

1994 ◽  
Vol 143 (1) ◽  
pp. 127-137 ◽  
Author(s):  
T-A Jaatinen ◽  
T-L Penttilä ◽  
A Kaipia ◽  
T Ekfors ◽  
M Parvinen ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Cellular Localization ◽  
Polycystic Ovarian Syndrome ◽  
Human Ovary ◽  
Α Subunit ◽  
Ribonucleic Acids ◽  
Theca Cells ◽  
Subunit Mrna ◽  
Normal Human ◽  
Ovarian Syndrome

Abstract We studied the cellular distribution of inhibin α, βA and βB mRNAs in the normal human ovary and in polycystic ovarian syndrome (PCOS) by in situ hybridization. Our results show that human granulosa cells express inhibin α, βA and βB subunit mRNAs, and theca cells express inhibin α and βA subunit mRNAs. The co-localization of α and βA mRNAs in theca cells supports the hypothesis that inhibin also has an autocrine function in these cells. We did not detect any inhibin subunit mRNA in the granulosa cells of atretic follicles, while theca cells also expressed α subunit mRNA in those follicles. The present findings suggest that the expression of inhibin subunits is regulated differently in human follicular granulosa and theca cells. It has been speculated that inhibin may be involved in the development of PCOS. Our results show that the cellular localization of inhibin subunit mRNAs is not disturbed in PCOS ovaries. Journal of Endocrinology (1994) 143, 127–137

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