Suppression of superoxide anion generation catalyzed by xanthine oxidase with alkyl caffeates and the scavenging activity

2016 ◽  
Vol 67 (3) ◽  
pp. 283-287 ◽  
Author(s):  
Noriyoshi Masuoka ◽  
Isao Kubo
Keyword(s):  
Xanthine Oxidase ◽  
Superoxide Anion ◽  
Scavenging Activity ◽  
Superoxide Anion Generation

Superoxide Anion Scavenging Effect of Coumarins

1996 ◽  
Vol 24 (01) ◽  
pp. 11-17 ◽  
Author(s):  
Wen-Shin Chang ◽  
Chun-Ching Lin ◽  
Shu-Chuan Chuang ◽  
Hsuch-Ching Chiang
Keyword(s):  
Cytochrome C ◽  
Xanthine Oxidase ◽  
Superoxide Anion ◽  
Folk Medicine ◽  
Ortho Position ◽  
Scavenging Activity ◽  
Percent Inhibition ◽  
Scavenging Effect ◽  
Hydroxy Groups ◽  
Phenolic Hydroxy

The superoxide scavenging effects of fifteen coumarins were tested on the xanthine-xanthine oxidase-cytochrome C system. The results showed that fraxetin(10) displayed the strongest activity, and its percent inhibition at 100, 10 and 1 μM were 100, 100 and 53.13% respectively. Esculetin(4) showed the second strongest activity resulting in percent inhibition at 100 and 10 μM were 87.16 and 52.38% respectively. Both fraxetin(10) and esculetin(4) have been isolated from the plant, Fraxinus bungeana DC (Oleaceae) which has been used in folk medicine as an analgesic and anti-inflammatory medicine. It seems that two phenolic hydroxy groups in the ortho position in the molecule of coumarins play an important role in scavenging activity.

Inhibitory effects of cardols and related compounds on superoxide anion generation by xanthine oxidase

2015 ◽  
Vol 166 ◽  
pp. 270-274 ◽  
Author(s):  
Noriyoshi Masuoka ◽  
Ken-ichi Nihei ◽  
Ayami Maeta ◽  
Yoshiro Yamagiwa ◽  
Isao Kubo
Keyword(s):  
Xanthine Oxidase ◽  
Superoxide Anion ◽  
Inhibitory Effects ◽  
Superoxide Anion Generation ◽  
Related Compounds

scholarly journals Meso-Dihydroguaiaretic Acid Ameliorates Acute Respiratory Distress Syndrome through Inhibiting Neutrophilic Inflammation and Scavenging Free Radical

Antioxidants ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 123
Author(s):  
Yen-Tung Lee ◽  
Yu-Li Chen ◽  
Yi-Hsuan Wu ◽  
Ih-Sheng Chen ◽  
Hsun-Shuo Chang ◽  
...  
Keyword(s):  
Acute Respiratory Distress Syndrome ◽  
Free Radical ◽  
Superoxide Anion ◽  
Distress Syndrome ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Human Neutrophils ◽  
Scavenging Activity ◽  
Superoxide Anion Generation

The pathogenesis of acute respiratory distress syndrome (ARDS) is very complex. Patients with ARDS still suffer high mortality rates. Infiltration and activation of neutrophils in lungs are critical pathogenic factors in ARDS. In this study, we demonstrate that meso-dihydroguaiaretic acid (MDGA), a natural lignan, inhibits inflammatory responses in human neutrophils and ameliorates ARDS in mice. MDGA inhibited superoxide anion generation and elastase release in various G-protein coupled receptor agonists-induced human neutrophils. However, MDGA did not alter superoxide anion generation and elastase activity in cell-free systems. These results suggest that the anti-inflammatory effects of MDGA are mediated by regulating cellular signals in human neutrophils. In consistent with this, MDGA suppressed phosphorylation of extracellular signal-regulated kinase and c-Jun N-terminal kinase in activated human neutrophils. Moreover, MDGA inhibited CD11b expression and adhesion in activated human neutrophils. Interestingly, MDGA reduced reactive oxygen species (ROS) generation but not superoxide anion generation in protein kinase C (PKC) activator-induced human neutrophils, suggesting that MDGA may also have ROS scavenging ability. Indeed, MDGA showed strong free radical scavenging activity in cell-free assays. Significantly, MDGA suppressed PKC-induced neutrophil extracellular trap formation. Additionally, treatment of MDGA attenuated neutrophil infiltration and lung damage on lipopolysaccharide-induced ARDS in mice. In conclusion, our results demonstrate that MDGA has anti-neutrophilic inflammatory effects and free-radical scavenging activity. We also suggest that MDGA has potential to serve as a lead for developing new therapeutics to treat ARDS.

Pterin-6-aldehyde, an Inhibitor of Xanthine Oxidase, Has Superoxide Anion Radical Scavenging Activity

1997 ◽  
Vol 233 (2) ◽  
pp. 447-450 ◽  
Author(s):  
Koji Watanabe ◽  
Toshiyuki Arai ◽  
Hiroko Mori ◽  
Shin-ichi Nakao ◽  
Toshinori Suzuki ◽  
...  
Keyword(s):  
Xanthine Oxidase ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Superoxide Anion Radical ◽  
Scavenging Activity

scholarly journals Role of Xanthine Oxidase in Passive Heymann Nephritis in Rats

1999 ◽  
Vol 10 (3) ◽  
pp. 538-544
Author(s):  
WILFRIED GWINNER ◽  
JENS PLASGER ◽  
RALF P. BRANDES ◽  
BIRGIT KUBAT ◽  
MATTHIAS SCHULZE ◽  
...  
Keyword(s):  
Xanthine Oxidase ◽  
Membranous Nephropathy ◽  
Superoxide Anion ◽  
Oxidase Activity ◽  
Superoxide Anion Generation ◽  
Xanthine Oxidase Activity ◽  
Heymann Nephritis ◽  
Quantitative Changes ◽  
Increased Activity

Abstract. Passive Heymann nephritis (PHN) in rats is a model of human membranous nephropathy characterized by formation of subepithelial immune deposits in the glomerular capillary wall and complement activation. Oxygen radicals have been implicated in the subsequent glomerular damage which leads to proteinuria. This study examines the involvement of xanthine oxidase in this process. Xanthine oxidase activity was increased nearly twofold in glomeruli isolated 1 and 12 d after induction of PHN, and this was associated with increased glomerular superoxide anion generation. Analysis of glomerular samples by Northern and Western blotting revealed no quantitative changes in xanthine oxidoreductase expression in PHN, suggesting conversion of xanthine dehydrogenase to the oxidase form as the cause of increased activity. Treatment of rats with tungsten, an inhibitor of xanthine oxidase, before induction of PHN resulted in a marked decrease in glomerular xanthine oxidase activity and superoxide anion generation, and decreased proteinuria by 80% (day 12: 423 ± 245 mg/d in PHN versus 78 ± 53 mg/d in tungsten-treated PHN animals, P < 0.01). These findings point to a pivotal role of xanthine oxidase in the pathophysiology of PHN and could be of importance in the therapy of human membranous nephropathy.

Mechanism of superoxide anion generation in the toxic red tide phytoplankton Chattonella marina: possible involvement of NAD(P)H oxidase

2000 ◽  
Vol 1524 (2-3) ◽  
pp. 220-227 ◽  
Author(s):  
Daekyung Kim ◽  
Atsushi Nakamura ◽  
Tarou Okamoto ◽  
Nobukazu Komatsu ◽  
Tatsuya Oda ◽  
...  
Keyword(s):  
Superoxide Anion ◽  
Red Tide ◽  
Chattonella Marina ◽  
Superoxide Anion Generation

scholarly journals An Anti-Inflammatory 2,4-Cyclized-3,4-Secospongian Diterpenoid and Furanoterpene-Related Metabolites of a Marine Sponge Spongia sp. from the Red Sea

Marine Drugs ◽  
2021 ◽  
Vol 19 (1) ◽  
pp. 38
Author(s):  
Chi-Jen Tai ◽  
Chiung-Yao Huang ◽  
Atallah F. Ahmed ◽  
Raha S. Orfali ◽  
Walied M. Alarif ◽  
...  
Keyword(s):  
Red Sea ◽  
Superoxide Anion ◽  
Dermal Fibroblast ◽  
Fibroblast Cell ◽  
Inhibitory Effect ◽  
Human Dermal Fibroblast ◽  
Superoxide Anion Generation ◽  
Potent Inhibitory Effect ◽  
Anti Inflammatory ◽  
New Compounds

Chemical investigation of a Red Sea Spongia sp. led to the isolation of four new compounds, i.e., 17-dehydroxysponalactone (1), a carboxylic acid, spongiafuranic acid A (2), one hydroxamic acid, spongiafuranohydroxamic acid A (3), and a furanyl trinorsesterpenoid 16-epi-irciformonin G (4), along with three known metabolites (−)-sponalisolide B (5), 18-nor- 3,17-dihydroxy-spongia-3,13(16),14-trien-2-one (6), and cholesta-7-ene-3β,5α-diol-6-one (7). The biosynthetic pathway for the molecular skeleton of 1 and related compounds was postulated for the first time. Anti-inflammatory activity of these metabolites to inhibit superoxide anion generation and elastase release in N-formyl-methionyl-leucyl phenylalanine/cytochalasin B (fMLF/CB)-induced human neutrophil cells and cytotoxicity of these compounds toward three cancer cell lines and one human dermal fibroblast cell line were assayed. Compound 1 was found to significantly reduce the superoxide anion generation and elastase release at a concentration of 10 μM, and compound 5 was also found to display strong inhibitory activity against superoxide anion generation at the same concentration. Due to the noncytotoxic activity and the potent inhibitory effect toward the superoxide anion generation and elastase release, 1 and 5 can be considered to be promising anti-inflammatory agents.

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