Involvement of HLA Antigens, Interleukin 2 Receptor and B-Cell Growth Factor in Interferon Action in Hairy Cell Leukemia

Leukemic B cells from seven patients with hairy cell leukemia (HCL), six of which contained the Tac antigen, were assayed in vitro for growth factor-mediated cell proliferation. The HCL cells showed typical phenotypic profiles by monoclonal antibody analysis. The tumor cells, which do not grow spontaneously in vitro, were found to proliferate in all but one case in response to partially purified B cell growth factor (BCGF) without anti-mu or Sac activation. Recombinant interleukin 2 however produced only a marginal response and could not support leukemic cell growth in vitro. BCGF, however, did stimulate in vitro cell growth and supported the establishment of continuous (greater than 60 d in vitro) in four of the seven HCL cases.

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Production of B cell growth factor(s) by neoplastic B cells from hairy cell leukemia patients

Blood ◽

10.1182/blood.v67.3.573.573 ◽

1986 ◽

Vol 67 (3) ◽

pp. 573-577 ◽

Cited By ~ 27

Author(s):

RJ Ford ◽

D Kwok ◽

J Quesada ◽

CG Sahasrabuddhe

Keyword(s):

T Cells ◽

B Cells ◽

Growth Factor ◽

Cell Growth ◽

B Cell ◽

Hairy Cell ◽

Cell Leukemia ◽

Human T Cells ◽

B Cell Growth Factor ◽

Normal Human

Abstract Recent studies have shown that normal human T cells contain a high- molecular-weight (mol wt) protein exhibiting B cell growth factor (BCGF) activity. Other studies have shown that virally transformed human B cells also secrete a high-mol-wt BCGF-like molecule in vitro. We have studied neoplastic B cells from patients with untreated hairy cell leukemia (HCL) to ascertain whether such cytoplasmic BCGF activity is present in the tumor cells. Studies on HCL cells from four patients indicated that BCGF-like activity was in fact present in the cytosolic extracts when tested on autochthonous HCL cells as well as on normal BCGF-dependent human B cell lines. Chromatographic analysis indicated that the BCGF activity from HCL cells was similar in mol wt as well as function to the normal T cell-derived cytosolic BCGF activity. These studies suggest that HCL cells contain and, in some cases, secrete a high-mol-wt growth factor that can be autostimulatory and appears to resemble a similar growth factor molecule found in normal human T cells.

Download Full-text

Production of B cell growth factor(s) by neoplastic B cells from hairy cell leukemia patients

Blood ◽

10.1182/blood.v67.3.573.bloodjournal673573 ◽

1986 ◽

Vol 67 (3) ◽

pp. 573-577

Author(s):

RJ Ford ◽

D Kwok ◽

J Quesada ◽

CG Sahasrabuddhe

Keyword(s):

T Cells ◽

B Cells ◽

Growth Factor ◽

Cell Growth ◽

B Cell ◽

Hairy Cell ◽

Cell Leukemia ◽

Human T Cells ◽

B Cell Growth Factor ◽

Normal Human

Recent studies have shown that normal human T cells contain a high- molecular-weight (mol wt) protein exhibiting B cell growth factor (BCGF) activity. Other studies have shown that virally transformed human B cells also secrete a high-mol-wt BCGF-like molecule in vitro. We have studied neoplastic B cells from patients with untreated hairy cell leukemia (HCL) to ascertain whether such cytoplasmic BCGF activity is present in the tumor cells. Studies on HCL cells from four patients indicated that BCGF-like activity was in fact present in the cytosolic extracts when tested on autochthonous HCL cells as well as on normal BCGF-dependent human B cell lines. Chromatographic analysis indicated that the BCGF activity from HCL cells was similar in mol wt as well as function to the normal T cell-derived cytosolic BCGF activity. These studies suggest that HCL cells contain and, in some cases, secrete a high-mol-wt growth factor that can be autostimulatory and appears to resemble a similar growth factor molecule found in normal human T cells.

Download Full-text