Comparison of tetrachromic VOF stain to other histochemical staining techniques for characterizing stromal soft and hard tissue components

2014 ◽  
Vol 89 (8) ◽  
pp. 545-551 ◽  
Author(s):  
C Belaldavar ◽  
S Hallikerimath ◽  
PV Angadi ◽  
AD Kale
Keyword(s):  
Histochemical Staining ◽  
Hard Tissue ◽  
Staining Techniques

Uneven distribution of preservative in kiln-dried sapwood lumber of Scots pine: Impact of wood structure and resin allocation

Holzforschung ◽  
2012 ◽  
Vol 66 (2) ◽  
Author(s):  
Sheikh Ali Ahmed ◽  
Margot Sehlstedt-Persson ◽  
Olov Karlsson ◽  
Tom Morén
Keyword(s):  
Scots Pine ◽  
Wood Preservative ◽  
Wood Properties ◽  
Histochemical Staining ◽  
Kiln Drying ◽  
Specific Distribution ◽  
Ray Cells ◽  
Staining Methods ◽  
Staining Techniques ◽  
Resin Canals

Abstract Scots pine (Pinus sylvestris L.) sapwood lumber was collected after kiln drying and preservative treatment with Celcure AC 800 (a copper-amine wood preservative). Distribution of the preservative throughout the lumber was visually examined. Not all, but some samples showed specific localized areas without any preservative distribution throughout their entire length. Those samples were assessed further for anatomical properties, specifically in impregnated and unimpregnated areas. Additional study was conducted on the morphological nature and redistribution of lipophilic extractives using three different histochemical staining methods. Intrinsic wood properties – especially the frequency of axial resin canals and the percentage of canals blocked – were found to be responsible for the irregular distribution of the preservative. Furthermore, the inability to create continuous and frequent interstitial spaces due to the collapse of thin-walled ray cells throughout the lumber resulted in un-even distribution of preservatives. Staining techniques were useful to localize places with more or less abundance of extractives (e.g., fats) in impregnated and unimpregnated wood, which varied considerably. Histochemical observations revealed information pertaining to the kiln dry specific distribution and redistribution of extractives between the areas. Moreover, resin reallocation and modification in ray parenchyma and resin canals induced by kiln drying would be another reason for the impregnation anomalies.

Studies on the structure and hatching of the eggs of Schistosoma mansoni

Parasitology ◽  
1970 ◽  
Vol 60 (1) ◽  
pp. 79-88 ◽  
Author(s):  
J. R. Kusel
Keyword(s):  
Schistosoma Mansoni ◽  
Young Patients ◽  
Histochemical Staining ◽  
Vitelline Membrane ◽  
Similar Treatment ◽  
Hatching Process ◽  
Staining Techniques ◽  
Egg Shell ◽  
And Function ◽  
Vacuolar Membranes

The structure and function of the vacuoles in the eggs of Schistosoma mansoni is unknown. In this study the eggs have been immersed in serum albumin and examined by phase-contrast optics. Under these conditions the vacuoles exhibit a definite internal structure, suggesting that they may be or may have been cellular. Histochemical staining techniques to detect the nature of the vacuolar contents revealed that the vacuoles stained heavily with iodine and gave a positive PAS reaction. This suggested the presence of carbohydrate. Stains for nucleic acids, protein and lipid were not taken up by the vacuoles. The egg shell stained with o–toluidine blue and with basic fuchsin uniformly, except for a thin irregular band which might indicate a possible line of weakness. The vacuolar membranes seem to be similar to the vitelline membrane in permeability to hydroxyl ions and it is possible that these membranes have a common origin. Plasmolysis studies with salts, urea, glycerol and sucrose showed that the vacuolar membranes have about the same permeability properties to these compounds as the other egg membranes. Glycerol treatment of the eggs until equilibrium is attained, followed by transfer of the eggs to water or saline results in a hatching of the eggs. In this, a dead mira-cidium is extruded from the shell. Similar treatment of eggs equilibrated with sucrose does not result in this hatching process. It is postulated that glycerol solubilizes a factor which aids the hatching process.I should like to thank the following: Dr S. A. Ibrahim, in whose department this work was carried out; Dr S. Dawood of the Stack Laboratory, Khartoum, who kindly made his microscope available for use; Mr J. R. Lauder for valuable discussions and suggestions; and many young patients in Khartoum Civil Hospital for stool samples and smiles.

Histological and histochemical structure of lingual salivaryglands in mole rat (Spalax leucodon)

2016 ◽  
Author(s):  
Nilgün Kuru ◽  
Kenan Çinar ◽  
Emel Demirbag ◽  
Ramazan Ilgün
Keyword(s):  
Salivary Glands ◽  
Histochemical Staining ◽  
Mucous Gland ◽  
Weak Reaction ◽  
Gland Cells ◽  
Mole Rat ◽  
Pas Reaction ◽  
Staining Techniques ◽  
Characteristic Features

The histological and histochemical structure of lingual salivary glands in mole rat (Spalax leucodon) were studied using histochemical staining techniques to provide information of its salivary glands. A total of five adult mole rat (Spalax leucodon) were used as the material. It was observed that serous and mucous glands are placed in the root of the tongue. It was detected that although the mucous gland cells were rich in terms of AB pH 0.5, 1.0, 2.5 and AF (+) mucosubstance, the PAS and KOH/PAS mucosubstances showed very weak reaction, and this mucosubstances were present at a very less amount in serous gland cells. In the PAS/AB staining, it was found that the serous and mucous gland cells showing only AB or only PAS reaction and also AB AB pH 2.5 (+) cells were found. The same findings were observed in serous glands by AF/AB staining. Consequently, the present study demonstrated characteristic features of the lingual salivary glands of the mole rat, and it revealed histological and histochemical data both in accordance with and different from that for the lingual salivary glands of mammals and other species.

Comparison of histochemical staining techniques for detecting mast cells in oral lesions

2019 ◽  
Vol 94 (6) ◽  
pp. 459-468 ◽  
Author(s):  
S. Mutsaddi ◽  
V. S. Kotrashetti ◽  
R. S. Nayak ◽  
S. M. Pattanshetty
Keyword(s):  
Mast Cells ◽  
Histochemical Staining ◽  
Oral Lesions ◽  
Staining Techniques

Identification of Marine Fish Eggs: A Biochemical Genetics Approach

1983 ◽  
Vol 40 (3) ◽  
pp. 361-369 ◽  
Author(s):  
Jarle Mork ◽  
Per Solemdal ◽  
Gunnar Sundnes
Keyword(s):  
Coastal Waters ◽  
Histochemical Staining ◽  
Pelagic Fish ◽  
Sampling Location ◽  
Biochemical Genetics ◽  
Fish Eggs ◽  
Specific Variation ◽  
Pelagic Eggs ◽  
Staining Techniques ◽  
Ldh Lactate Dehydrogenase

We studied inter- and intra-specific variation at LDH (lactate dehydrogenase; EC 1.1.1.27) loci in 11 gadoid and 6 flatfish species regularly occurring in Norwegian coastal waters by means of isoelectric focusing and histochemical staining techniques. No two species showed identical locus A (white skeletal muscle predominating) zymograms. Polymorphism at LDH A was observed in haddock (Gadus aeglefinus), coalfish (G. virens), Norway pout (G. esmarkii), four-bearded rockling (Onos cimbrius), and dab (Limanda limanda). The allelic nature of observed LDH variants was confirmed by controlled crossings in cod (G. morhua, locus B), Norway pout (locus A), and haddock (locus A). Analyses of artificially fertilized eggs (in four species) and prenatal eggs from ripening ovaries (in 10 species) revealed a general predominance of locus A products. The maternal LDH A activity present in newly released eggs was overtaken by embryo-synthesized enzyme 1–2 d after fertilization. No unique embryonic LDH loci were observed. LDH A zymograms thus appear to be useful as a practically diagnostic tool for the identification of the pelagic fish eggs usually found in these waters. The identification method, applied to samples of pelagic eggs collected at the coast of northern Norway during March–May 1981 and 1982, showed that in a total of ~ 1500 eggs, the following species were represented: cod, Norway pout, haddock, coalfish, dab, plaice (Pleuronectes platessa), and long rough dab (Hippo-glossoides plates-soides). The species composition in these egg samples varied considerably with sampling location and sampling depth.Key words: fish egg identification, biochemical genetics, tissue enzymes, gadoids, flatfishes

scholarly journals THE KREBS CYCLE IN GLOMERULI OF NORMAL RAT KIDNEY AND IN COMPENSATORY HYPERTROPHY

1967 ◽  
Vol 15 (1) ◽  
pp. 32-37 ◽  
Author(s):  
WIKTOR W. NOWINSKI ◽  
ANDRZEJ PIGOŃ
Keyword(s):  
Normal Cell ◽  
Enzyme System ◽  
Rat Kidney ◽  
Succinic Dehydrogenase ◽  
Krebs Cycle ◽  
Histochemical Staining ◽  
Compensatory Hypertrophy ◽  
Cell Functions ◽  
Normal Rat ◽  
Staining Techniques

The Krebs cycle in glomeruli of rat kidney was studied under normal conditions and in compensatory hypertrophy after unilateral nephrectomy. By using substrates of the cycle and measuring with the Cartesian diver technique the increase in oxygen uptake, it was established that the entire enzyme system is present, including succinic dehydrogenase. The latter could not be demonstrated by other authors who used older histochemical staining techniques. In compensatory hypertrophy it was thought sufficient to study activites of two enzymes only, isocitric dehydrogenase and succinic dehydrogenase. No change in the activity of either dehydrogenase was found. The conclusion was drawn that in hypertrophy, the glomeruli simply swell and serve as ultrafilters; the Krebs cycle is present, but produces ATP only for the maintenance of normal cell functions and thus does not undergo an increase in activity.

Freeze Fracture at the Turn of the Century: Techniques for Visualizing and Labeling Tissues, Cells, and Molecules

1997 ◽  
Vol 3 (S2) ◽  
pp. 345-346
Author(s):  
Thomas Yasumura ◽  
John E. Rash
Keyword(s):  
Freeze Fracture ◽  
Histochemical Staining ◽  
Three Dimensions ◽  
Fracture Plane ◽  
Membrane Composition ◽  
Ependymal Layer ◽  
Recent Developments ◽  
Staining Techniques ◽  
Areas Of Interest ◽  
Macromolecular Architecture

Freeze-fracture techniques have contributed much to our understanding of membrane composition and macromolecular architecture. However, further substantive contributions from freeze fracture have seemed unlikely because: a) replicas typically fragmented, destroying histological orientation within samples; b) histochemical staining techniques were thought to be incompatible with bleach- or acid-cleaned freeze-fracture replicas; c) the limit of resolution was presumed to be 3-5 nm; and d) there were no methods for directing the fracture plane to specific components within a tissue. Recent developments in “grid-mapped freeze fracture” and freeze-fracture immunocytochemistry have addressed each of these limitations.Using grid-mapped freeze fracture, samples are mapped in three dimensions using confocal microscopy before freeze-fracture (Fig. A), as well as after replication and stabilization in Lexan plastic on a gold “Finder” grid (Fig. B). After replica cleaning, areas of interest are correlated in confocal and TEM images —— for example, cilia of the ependymal layer of rat spinal cord (Fig. C).

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