Grimelius' Silver Stain for Endocrine Cell Granules, as Shown by Electron Microscopy

1971 ◽  
Vol 46 (1) ◽  
pp. 7-13 ◽  
Author(s):  
Gabriele Vassallo ◽  
Carlo Capella ◽  
Enrico Solcia
1980 ◽  
Vol 28 (3) ◽  
pp. 1009-1013
Author(s):  
T Butler ◽  
M Aikawa ◽  
A Habte-Michael ◽  
C Wallace

The removal of Borrelia spirochetes from the blood in relapsing fever was studied by examining patients' blood phagocytic cells with the Dieterle silver stain. Polymorphonuclear leukocytes ingested Borrelia at increased rates for several hours after antibiotic treatment, during which time the total numbers of circulating plasma spirochetes were decreasing. Incubation of infected blood at 37 degrees C for 2 h resulted in a progressive increase in phagocytosis. Addition of penicillin G and tetracycline to infected blood caused a further enhancement of phagocytosis. Electron microscopy of polymorphonuclear leukocytes revealed spirochetes in phagosomes. These results indicated that blood polymorphonuclear leukocytes have a prominent role in removing Borrelia from the plasma and suggested that antibiotics act by altering the surface of spirochetes to render them more susceptible to phagocytosis.


Author(s):  
Robert L. Corbett

The ammoniacal silver stain for light microscopy of plastic embedded sections has been adapted for use in electron microscopy. Since the silver will stain even very thin sections, i.e., silver and gold for light microscopy, and silver deposits are sufficiently electron dense to be seen in the electron microscope, the results are very useful for correlating light and electron microscopy. Compared to the conventional stains for electron microscopy which usually take over one-half hour, the silver procedure can be done in five minutes or less and thus provides a quick look at sections This stain has more contrast, so it is especially good for low power electron microscopy. The ability of the silver to stain very thin sections enables a correlation between light and electron microscopy in three ways. First, thin sections can be stained with silver on a glass slide and compared with immediately adjacent thin sections on grids stained the usual way for electron microscopy.


Author(s):  
B.L Giammara ◽  
R.L. Hopfer ◽  
P.E. Yates ◽  
J.S. Hanker

Although a number of cytochemical methods have been introduced for nuclei at the ultrastructural level, many of these are topographic, i.e. identify nuclei rather than nuclear chromatin and DNA. An improved silver method for the Feulgen reaction for the light and electron microscopy of DNA was introduced in 1984 (FETS reaction). It required application of the silver methenamine for 15 min. under UV-irradiation and subsequent development of the stain overnight in glycerine. This type of methodology has been considerably accelerated by the application of silver solutions, especially silver methenamine solutions prepared from the solid, under microwave(MW) irradiation. The current procedure employs 1 min. formalin fixation of a smear of the sample on a slide or coverslip. The fixed smear is then rinsed 1 hr. in buffer. Aldehyde or pseudoaldehyde groups of apurinic acids are generated by the action of 2.5N HCl for 10 min. on the DNA. These groups are then condensed with thiocarbohydrazide and the resulting product reacted with silver methenamine solution under MW irradiation for 4 min.


Author(s):  
S. K. Aggarwal ◽  
P. McAllister ◽  
R. W. Wagner ◽  
B. Rosenberg

Uranyl acetate has been used as an electron stain for en bloc staining as well as for staining ultrathin sections in conjunction with various lead stains (Fig. 1). Present studies reveal that various platinum compounds also show promise as electron stains. Certain platinum compounds have been shown to be effective anti-tumor agents. Of particular interest are the compounds with either uracil or thymine as one of the ligands (cis-Pt(II)-uracil; cis-Pt(II)-thymine). These compounds are amorphous, highly soluble in water and often exhibit an intense blue coloration. These compounds show enough electron density to be used as stains for electron microscopy. Most of the studies are based on various cell lines (human AV, cells, human lymphoma cells, KB cells, Sarcoma-180 ascites cells, chick fibroblasts and HeLa cells) while studies on tissue blocks are in progress.


Author(s):  
Gunter F. Thomas ◽  
M. David Hoggan

In 1968, Sugimura and Yanagawa described a small 25 nm virus like particle in association with the Matsuda strain of infectious canine hepatitis virus (ICHV). Domoto and Yanagawa showed that this particle was dependent on ICHV for its replication in primary dog kidney cell cultures (PDK) and was resistant to heating at 70°C for 10 min, and concluded that it was a canine adeno-associated virus (CAAV). Later studies by Onuma and Yanagawa compared CAAV with the known human serotypes (AAV 1, 2, 3) and AAV-4, known to be associated with African Green Monkeys. Using the complement fixation (CF) test, they found that CAAV was serologically related to AAV-3 and had wide distribution in the dog population of Japan.


Author(s):  
L. D. Ackerman ◽  
S. H. Y. Wei

Mature human dental enamel has presented investigators with several difficulties in ultramicrotomy of specimens for electron microscopy due to its high degree of mineralization. This study explores the possibility of combining ion-milling and high voltage electron microscopy as a means of circumventing the problems of ultramicrotomy.A longitudinal section of an extracted human third molar was ground to a thickness of about 30 um and polarized light micrographs were taken. The specimen was attached to a single hole grid and thinned by argon-ion bombardment at 15° incidence while rotating at 15 rpm. The beam current in each of two guns was 50 μA with an accelerating voltage of 4 kV. A 20 nm carbon coating was evaporated onto the specimen to prevent an electron charge from building up during electron microscopy.


Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.


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