Teflon Membrane, an Easily Removable Cover Slip for Squash Preparations

1972 ◽  
Vol 47 (4) ◽  
pp. 209-210 ◽  
Author(s):  
Peter Lorber ◽  
Walter H. Muller
Keyword(s):  
Cover Slip ◽  
Teflon Membrane

Biological and biomedical applications of collagenous biomaterials

1990 ◽  
Vol 48 (3) ◽  
pp. 856-857
Author(s):  
Yasushi P. Kato ◽  
Michael G. Dunn ◽  
Frederick H. Silver ◽  
Arthur J. Wasserman
Keyword(s):  
Biomedical Applications ◽  
Soft Tissues ◽  
Collagen Fibers ◽  
Bone Collagen ◽  
Cover Slip ◽  
Fibroblast Migration ◽  
Cellular Expression ◽  
Defect Repair

Collagenous biomaterials have been used for growing cells in vitro as well as for augmentation and replacement of hard and soft tissues. The substratum used for culturing cells is implicated in the modulation of phenotypic cellular expression, cellular orientation and adhesion. Collagen may have a strong influence on these cellular parameters when used as a substrate in vitro. Clinically, collagen has many applications to wound healing including, skin and bone substitution, tendon, ligament, and nerve replacement. In this report we demonstrate two uses of collagen. First as a fiber to support fibroblast growth in vitro, and second as a demineralized bone/collagen sponge for radial bone defect repair in vivo.For the in vitro study, collagen fibers were prepared as described previously. Primary rat tendon fibroblasts (1° RTF) were isolated and cultured for 5 days on 1 X 15 mm sterile cover slips. Six to seven collagen fibers, were glued parallel to each other onto a circular cover slip (D=18mm) and the 1 X 15mm cover slip populated with 1° RTF was placed at the center perpendicular to the collagen fibers. Fibroblast migration from the 1 x 15mm cover slip onto and along the collagen fibers was measured daily using a phase contrast microscope (Olympus CK-2) with a calibrated eyepiece. Migratory rates for fibroblasts were determined from 36 fibers over 4 days.

scholarly journals Mechanical properties of very thin cover slip glass disk

2001 ◽  
Vol 24 (2) ◽  
pp. 151-155 ◽  
Author(s):  
A. Seal ◽  
A. K. Dalui ◽  
M. Banerjee ◽  
A. K. Mukhopadhyay ◽  
K. K. Phani
Keyword(s):  
Mechanical Properties ◽  
Cover Slip ◽  
Glass Disk

scholarly journals DEVELOPMENT OF FLOW INJECTION METHOD FOR ONLINE DETERMINATION OF THIOCYANATE BASED ON OXIDATION BY PERMANGANATE

2010 ◽  
Vol 10 (2) ◽  
pp. 167-171 ◽  
Author(s):  
Hermin Sulistyarti ◽  
Spas D. Kolev ◽  
Stephanie Lim
Keyword(s):  
Detection Limit ◽  
Thyroid Gland ◽  
Flow Injection ◽  
Hydrogen Cyanide ◽  
Figures Of Merit ◽  
Sample Throughput ◽  
Teflon Membrane ◽  
Developing Method ◽  
Analytical Figures Of Merit

The importance of developing method for thiocyanate becomes obvious, because thiocyanate can inhibit iodine uptake of thyroid gland leading to mumps disease. In this work, thiocyanate is oxidized by permanganate in the acid donor stream to cyanide, which is directly converted to hydrogen cyanide. Then, hydrogen cyanide diffuses through a Teflon membrane into acceptor stream containing nickel(II) in ammoniacal buffer to form tetracyanonickelate(II) which is detected spectrophotometrically at 267 nm. Analytical figures of merit were linear up to 50 mg L-1 for thiocyanate, with RSD of 1.34%, and detection limit of 0.07 mg L-1, respectively. Interfering anions were eliminated under stoichiometric amount of permanganate and sample throughput was 20 h-1. The method was validated for determining thiocyanate samples from synthetic and gold process waters with satisfactory results.   Keywords: Thiocyanate, flow injection, permanganate, spectrophotometry

Evaluation of flow injection analysis for determination of urea in sheep's and cow's milk

2002 ◽  
Vol 50 (3) ◽  
pp. 263-271 ◽  
Author(s):  
Martina Baumgartner ◽  
Martina Flöck ◽  
Petra Winter ◽  
Keyword(s):  
Bovine Milk ◽  
Reference Method ◽  
Measurement Procedure ◽  
Ammonium Ions ◽  
Routine Diagnostics ◽  
Urea Content ◽  
Teflon Membrane ◽  
Accuracy And Precision ◽  
Spectral Photometry

Difficulties in measuring the urea content in sheep's milk often occur with spectral photometry due to the high protein and fat concentrations of the milk. In this study an enzymatic flow procedure (QuickChem 8000 Ion Analyser, Lachat Instruments, Milwaukee, USA) to determine the urea content in ovine and bovine milk was evaluated. Urea content is determined by the Berthelot reaction after splitting it enzymatically with urease. The free ammonia diffuses through a teflon membrane into a stream of reagent solutions. Detection takes place by means of a reaction between the ammonium ions with hypochlorite and salicylate producing a green colour, which is measured spectrometrically in a flow meter at 660 nm. By using a diffusion cell chemical deproteinisation of milk is not necessary and capacity is high. The assessed procedure exhibited high accuracy and precision and reached a sample capacity of 55 samples an hour. Storage of the milk samples for several days as well as chemical preservation with bronopol had no effect on the measurement procedure. Due to the complexity of the apparatus and the costs associated therewith, the device proves less suitable for routine diagnostics but rather serves as a reference method for the measurement of urea concentration in milk.

scholarly journals THE CIRCUMFUSION SYSTEM FOR MULTIPURPOSE CULTURE CHAMBERS

1967 ◽  
Vol 32 (1) ◽  
pp. 89-112 ◽  
Author(s):  
George G. Rose
Keyword(s):  
Tissue Culture ◽  
Chick Embryo ◽  
Mechanical System ◽  
Environmental Conditions ◽  
The Other ◽  
Cover Slip

A self-contained mechanical system for circulating nutrient fluid through 12 tissue culture chambers is described in detail. This system utilizes nonperforated cellophane membranes in the chambers which separate the circulating nutrient from the tissue culture environments. The nutrient, therefore, is dialyzed through the cellophane of each chamber; some cell products are retained in the microenvironment between the closely apposed cellophane and cover slip, whereas the other cell products move from chamber to chamber in the circulating nutrient. The resultant environmental conditions directed by the circumfusion systems are highly favorable for maintaining the differentiation of chick embryo tissues over protracted periods; a number of micrographs are shown.

An easily made, inexpensive, cover slip carrier for critical point drying

1988 ◽  
Vol 8 (2) ◽  
pp. 231-232 ◽  
Author(s):  
Brian A. Hollander ◽  
Timothy K. Maugel ◽  
Dale B. Bonar
Keyword(s):  
Critical Point ◽  
Cover Slip ◽  
Critical Point Drying
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