Laboratory-Based Ex Vivo Assessment of Stratum Corneum Function

2002 ◽  
pp. 553-570
Keyword(s):  
Stratum Corneum ◽  
Ex Vivo

scholarly journals New model of biological membrane (shed snakeskin) for studies of antioxidant activity in photoprotective formulation/ Novo modelo de membrana biológica (ecdise de pele de cobra) para estudos de atividade antioxidante em formulação fotoprotetora

2021 ◽  
Vol 7 (4) ◽  
pp. 43214-43236
Author(s):  
Thamires Batello Freire ◽  
Orlando Rodrigues Júnior ◽  
Claudinéia Aparecida Sales de Oliveira Pinto ◽  
Cassiano Carlos Escudeiro ◽  
André Rolim Baby ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Stratum Corneum ◽  
Ex Vivo ◽  
Electron Paramagnetic Resonance Spectroscopy ◽  
Biological Membrane ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Oxidative Capacity ◽  
Resonance Spectroscopy

Antioxidants of natural origin are used in medicines and cosmetics with several benefits, such as: photoprotective action, anti-aging, moisturizing and anti-pollutant. The human epidermis has an important barrier effect and limited anti-oxidative capacity, so studies with the epidermis is essential. Shed snakeskin (SS) is composed of the stratum corneum and provide a barrier like human stratum corneum. This alternative does not show a tendency to microbiological degradation and can be considered ecologically correct. This study intends to present, in an innovative way, the Electron Paramagnetic Resonance spectroscopy (EPR) and The Forster Resonance Energy Transfer (FRET) were employed to evaluate the natural antioxidant substances (Resveratrol/ RES 3.0 w/w and Ferulic acid/ FA 1.0 w/w) associated with organic sunscreens ingredients (Ethylhexyl Methoxycinnamate/ EHMC 10.0%w/w and Butyl Methoxydibenzoylmethano/ BMBM 5.0%w/w in a photoprotective emulsion (PB). Furthermore, the use of SS seedlings as a possible alternative to the use of human or animal skin ex-vivo. RES and FA can absorb the energy emitted by the EHMC in FRET, preventing the passage through the triplet state, favoring the photostability of this sunscreen, the same not ocorred with the BMBM. Antioxidant activity of the photoprotective formulations was evaluated in vitro by the percentual inhibition of the radical 2,2-diphenyl-1-picrihydrazyl (DPPH•). The antioxidant activity with RES, 97.0% inhibition of DPPH• in the PB, was higher than PB + FA (91.0%), however the concentration of RES in PB was higher than FA. The sample SS + PB + FA was the one with the lowest number of free radicals after irradiation, which corroborated the high percentage of radical inhibition in vitro and it was the better association with the photoprotective formulation. 

scholarly journals Mangiferin and Hesperidin Transdermal Distribution and Permeability through the Skin from Solutions and Honeybush Extracts (Cyclopia sp.)—A Comparison Ex Vivo Study

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6547
Author(s):  
Anna Hering ◽  
Jadwiga Renata Ochocka ◽  
Helena Baranska ◽  
Krzysztof Cal ◽  
Justyna Stefanowicz-Hajduk
Keyword(s):  
Stratum Corneum ◽  
Ex Vivo ◽  
Water Solution ◽  
Ethanol Extract ◽  
Ethanol Solution ◽  
Dorsal Skin ◽  
Photodiode Array ◽  
Array Detection ◽  
Electrochemical Array ◽  
Ex Vivo Study

Polyphenolic compounds—mangiferin and hesperidin—are, among others, the most important secondary metabolites of African shrub Cyclopia sp. (honeybush). The aim of this study was to compare the percutaneous absorption of mangiferin and hesperidin from solutions (water, ethanol 50%, (v/v)) and extracts obtained from green and fermented honeybush (water, ethanol 50%, (v/v)). Research was performed with the Bronaugh cells, on human dorsal skin. The mangiferin and hesperidin distributions in skin layers (stratum corneum, epidermis, and dermis) and in acceptor fluid (in every 2, 4, 6, and 24 h) were evaluated by HPLC–Photodiode Array Coulometric and Coulometric Electrochemical Array Detection. The transdermal distribution of hesperidin was also demonstrated by fluorescence microscopy. Results indicated that mangiferin and hesperidin were able to cross the stratum corneum and penetrate into the epidermis and dermis. An advantage of hesperidin penetration into the skin from the water over ethanol solution was observed (451.02 ± 14.50 vs. 357.39 ± 4.51 ng/cm2), as well as in the mangiferin study (127.56 ± 9.49 vs. 97.23 ± 2.92 ng/cm2). Furthermore, mangiferin penetration was more evident from nonfermented honeybush ethanol extract (189.85 ± 4.11 ng/cm2) than from solutions. The permeation of mangiferin and hesperidin through the skin to the acceptor fluid was observed regardless of whether the solution or the honeybush extract was applied. The highest ability to permeate the skin was demonstrated for the water solution of hesperidin (250.92 ± 16.01 ng/cm2), while the hesperidin occurring in the extracts permeated in a very low capacity. Mangiferin from nonfermented honeybush ethanol extract had the highest ability to permeate to the acceptor fluid within 24 h (152.36 ± 8.57 ng/cm2).

scholarly journals Multiphoton polarization imaging of the stratum corneum and the dermis in ex-vivo human skin

2003 ◽  
Vol 11 (25) ◽  
pp. 3377 ◽  
Author(s):  
Yen Sun ◽  
Jiunn-Wen Su ◽  
Wen Lo ◽  
Sun-Jan Lin ◽  
Shiou-Hwa Jee ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Human Skin ◽  
Ex Vivo ◽  
Polarization Imaging

scholarly journals Topically Applied Ceramides Interact with the Stratum Corneum Lipid Matrix in Compromised Ex Vivo Skin

2018 ◽  
Vol 35 (3) ◽  
Author(s):  
Tineke Berkers ◽  
Dani Visscher ◽  
Gert S. Gooris ◽  
Joke A. Bouwstra
Keyword(s):  
Stratum Corneum ◽  
Ex Vivo ◽  
Lipid Matrix ◽  
Stratum Corneum Lipid

An ex vivo methodology to assess the lipid peroxidation in stratum corneum

2009 ◽  
Vol 97 (2) ◽  
pp. 71-76 ◽  
Author(s):  
Cristina Alonso ◽  
Clara Barba ◽  
Laia Rubio ◽  
Sonya Scott ◽  
Anna Kilimnik ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Stratum Corneum ◽  
Ex Vivo

scholarly journals Alteration of the Structure of Human Stratum Corneum Facilitates Transdermal Delivery

2014 ◽  
Vol 8 (1) ◽  
pp. 72-79
Author(s):  
Anne Mundstock ◽  
Rawad Abdayem ◽  
Fabrice Pirot ◽  
Marek Haftek
Keyword(s):  
Stratum Corneum ◽  
Ex Vivo ◽  
Morphological Changes ◽  
Skin Permeation ◽  
Ultrastructural Changes ◽  
Active Components ◽  
Transmission Electron ◽  
Liquid Systems ◽  
Hydrophilic Compounds ◽  
Pharmacologically Active

Transdermal transport of pharmacologically active components into the skin depends on the ability of galenic formulations to overcome the stratum corneum (SC) barrier. Microemulsions (ME) are thermodynamically stable liquid systems composed of water, oil and surfactants which may be used for skin permeation and enhance penetration of hydrophobic as well as hydrophilic compounds. We investigated using transmission electron microscopy the effect of ME on human epidermis ex vivo, in order to establish relationship between the type of ME, i.e.: oil-in-water, water-in-oil, gellike, thickened or not with colloidal silica, and the ultrastructural changes in SC barrier resulting from their topical application. ME induced various degrees of dissociation of the SC. The intercellular lipid matrix in the SC became disorganized, which contributed to the separation of corneocytes. This effect was intensified with the increasing oil content in the ME and also when ME were applied under occlusion. The observed morphological changes were in agreement with the increased permeability of ME-treated skin to both lipophilic and hydrophilic compounds reported in the literature. Severe deterioration of the SC barrier induced with the selected ME makes them suitable for selected indications only.

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