The plant Justicia simplex D.Don is traditional herb, people used it in different diseases, they treat fever and burning of the body, strengthen the lungs, the teeth and stop vomiting. The plant exhibited more promising bacterial activity against various microorganisms. Hence, in the present study this plant is also tested against the dental pathogens. The plant was extracted from four different solvents was tested for the presence of phytochemicals in it. After analyzing, its antimicrobial activity is tested against fungi and bacteria. Then a dental carrier Streptococcus aureus, Klebsiella pneumonia, Escherichia coli and Pseudomonas aeruginosa which was collected from the K.S.R. Institute for Dental Science and Research. The isolates were used to check the activity against Justicia simplex D.Don. Based on the positive result obtained against various analyses, it is formulated successfully into a tooth paste. The present study on Phytochemical screening of Justicia simplex D.Don a valuable medicinal plant extract against Dental pathogens enables the use of this plant for the formulation of tooth powder against dental pathogens. The result is more effective against dental pathogens by herbal tooth powder
DNA Barcoding, phytochemical screening and antimicrobial activity of Rhododendron arboreum, a high altitudinal medicinal plant from Nepal
