scholarly journals Estudio piloto de la prueba de percepción musical camp en implantados y normoyentes

2012 ◽  
Vol 21 (2) ◽  
pp. 87-97
Author(s):  
Adelaida Plaza Ruiz ◽  
Juan Manuel García ◽  
Juan Carlos Izquierdo
Keyword(s):  
Camp Test

En la búsqueda de una prueba de percepción musical estandarizada y aplicable en español, se evaluó el CAMP test creado en inglés por la Universidad de Washington para implantados y normoyentes. Objetivo: determinar las dificultades de su aplicación cuando el idioma nativo es español. Método: estudio de corte transversal con tres pruebas de percepción musical: umbral de tonos, percepción de melodías y timbre musical. Para correr el programa se tradujeron al español los comandos. Resultados: se reclutaron cinco pacientes, tres de ellos hombres (60%). La edad mediana fue 42 años (RI de 40 a 43). El tiempo mediano de deprivación fue de 60 meses (RI 36 a 60). La mediana del tiempo de uso del implante fue de doce meses (RI 6 a 108). El resultado de detección del tono por frecuencias para el de 262 Htz la mediana fue de 3.61 (RI 2.17 a 4.28), para 330 Htz 3.72 (RI 2.94 a 4.28) y para 391 Htz 5.5 (RI 4.17 a 8.56). El porcentaje de reconocimiento con las melodías presentó una mediana de 8,33% (RI 5,56% a 13,89%) y el del timbre musical, medido por el reconocimiento de los instrumentos, 33,3% (RI 20,83 a 45,83%). Los controles fueron siete individuos sanos de los cuales cuatro eran mujeres (57,14%), con edad mediana de 31 años (RI 18 a 44). En la percepción del tono el resultado de detección del umbral por frecuencias fue: para el de 262 Htz de 1 (RI 0.67 a 3.39), para 330 Htz 0.94 (RI 0.5 a 4.39) y para 391 Htz 0.72 (RI 0.61 a 3.22). El porcentaje de reconocimiento con las melodías presentó una mediana de 69% (RI 33,3 a 83,3%) y el del timbre musical 62,5% (RI 45,8 a 75%). La mayoría de los sujetos evaluados (10 de 12) realizaron la prueba sin ayuda de los examinadores, solo dos la requirieron para el manejo del programa, pero entendieron y pudieron completarla. De las tres características musicales evaluadas, la identificación de las melodías fue la más difícil. Conclusión: la prueba CAMP de percepción musical resulta útil fácil de aplicar en normoyentes y usuarios de implante coclear cuyo idioma nativo sea español, cuando se dispone de cartillas con traducción de los comandos.

Evaluation of spot CAMP test for identification of group B streptococci.

1986 ◽  
Vol 24 (2) ◽  
pp. 296-297 ◽  
Author(s):  
H B Ratner ◽  
L S Weeks ◽  
C W Stratton
Keyword(s):  
Group B Streptococci ◽  
Camp Test ◽  
Group B

Comparison of MICRO-ID Listeria Method with Conventional Biochemical Methods for Identification of Listeria Isolated From Food and Environmental Samples: Collaborative Study

1993 ◽  
Vol 76 (4) ◽  
pp. 831-838 ◽  
Author(s):  
Don L Higgins ◽  
Barbara J Robison ◽  
◽  
S Bailey ◽  
C Hagen ◽  
...  
Keyword(s):  
Environmental Samples ◽  
Collaborative Study ◽  
Physiological Saline ◽  
Test Strip ◽  
Gram Staining ◽  
Laboratory Cultures ◽  
Biochemical Identification ◽  
Inaccurate Reading ◽  
Biochemical Analyses ◽  
Camp Test

Abstract Fourteen laboratories participated in a collaborative study to evaluate the ability of the MICRO-ID Listeria identification method to correctly identify Listeria isolated from food and environmental sources. Each collaborator received 60 isolates consisting of 51 Listeria and 9 non-Listeria cultures. All isolates were identified by conventional biochemical analyses in the principal laboratory. Cultures were checked for purity by Gram staining and examined for oxidase and catalase activities. Only Gram positive, oxidase negative, catalase positive cultures were tested with the method. Colonies from trypticase soy agar with 0.6% yeast extract were suspended in 4.6 ml_ physiological saline to a MacFarland No. 1 turbidity standard and used to inoculate the test strip. In addition, the hemolytic reaction of each isolate was determined by using the Christie-Atkins-Munch-Peterson (CAMP) test and by stabbing sheep blood agar. Identification of Listeria is based on the octal code obtained from the strip and the hemolytic reaction of the isolate. The MICRO-ID Listeria method agreed with conventional biochemical identification for 98.0% of L. monocytogenes, 77.1% of L. seeligeri, 98.0% of L ivanovii, 96.4% of L. grayi/L. murrayi, 73.9% of L. welshimeri, and 100% of L innocua isolates. A large percentage of errors in identification of the L. seeligeri and L ivanovii cultures was caused by inaccurate reading of the CAMP and hemolysis tests rather than errors in the test strip. The method was adopted first action by AOAC International.

Identification and Enumeration of Beta-Hemolytic Listeria monocytogenes in Naturally Contaminated Dairy Products

1988 ◽  
Vol 71 (3) ◽  
pp. 673-675
Author(s):  
Atin R Datta ◽  
Barry A Wentz ◽  
Walter E Hill
Keyword(s):  
Listeria Monocytogenes ◽  
Dairy Products ◽  
Dna Probe ◽  
Milk Samples ◽  
Unpasteurized Milk ◽  
Pure Cultures ◽  
Camp Test

Abstract A DNA probe was used to identify hemolytic Listeria monocytogenes in naturally contaminated dairy products: unpasteurized milk, ricotta cheese, and imported semisoft cheeses. Of 34 milk samples, 12 were suspected to contain hemolytic L. monocytogenes; 1 contained >6000 viable organisms/g. The ricotta cheese, although temperature-abused, had a titer of 3.6 x 10-6 beta-hemolytic L. monocytogenes cells/g, whereas the semisoft cheeses reached a maximum of 5.6 x 10-6 cells/g. Pure cultures of L. monocytogenes isolated from both types of cheese were found positive by the CAMP test and the DNA probe

PREPARATION AND TITRATION OF CRUDE STAPHYLOCOCCAL BETA HEMOLYSIN FOR USE IN T.K.T. MEDIUM1,2

1968 ◽  
Vol 31 (6) ◽  
pp. 171-173 ◽  
Author(s):  
G. E. Ward ◽  
D. S. Postle
Keyword(s):  
Method Of Production ◽  
Camp Test

A simple, effective method for preparation and titration of crude staphylococcal β hemolysin is presented. Factors which affect the results are discussed. This method of production and titration is useful for laboratories which us T.K.T. medium for the identification of CAMP test-positive streptococci in bulk, bucket or quarter milk.

Virulence of Listeria monocytogenes Serovars and Listeria spp. in Experimental Infection of Mice

1991 ◽  
Vol 54 (12) ◽  
pp. 917-921 ◽  
Author(s):  
ALAIN MENUDIER ◽  
CLAUDINE BOSIRAUD ◽  
JEAN-ALBERT NICOLAS
Keyword(s):  
Listeria Monocytogenes ◽  
Peritoneal Lavage ◽  
Lavage Fluid ◽  
Fixed Number ◽  
Peritoneal Lavage Fluid ◽  
Listeria Ivanovii ◽  
Wild Strains ◽  
Camp Test ◽  
Listeria Seeligeri ◽  
Listeria Welshimeri

Wild strains of Listeria monocytogenes, Listeria ivanovii, Listeria seeligeri, Listeria innocua, and Listeria welshimeri were isolated from infected animals and foodstuffs. Their virulence was tested in Swiss mice after intraperitoneal injection of a fixed number of organisms. The presence of hemolysin was determined using the CAMP test. Bacteria were enumerated in peritoneal lavage fluid, liver, and spleen. Spleen weights were measured, and the presence of L. monocytogenes in the brain was also investigated. L. innocua, L. seeligeri, and L. welshimeri were not found to be pathogenic for mice. L. ivanovii was detected in liver, spleen, and peritoneal lavage fluid but at lower levels than L. monocytogenes (p<0.001). The pathogenic capabilities of four different serovars of L. monocytogenes (4b, 1/2a, 1/2b, 1/2c) were compared. Serovars l/2b and l/2c, which are frequently isolated from foodstuffs, were found to colonize the liver and spleen to a lesser extent than serovar 4b (p<0.01 and <0.001 respectively). The behavior of serovar l/2a, the most commonly isolated from foodstuffs, was strain dependent. Two out of the four strains tested were strongly hemolytic and were as virulent as strains of serovar 4b, while the other two were weakly hemolytic, and avirulent like L. innocua. These results could account for the relatively small number of human Listeria infections due to L. monocytogenes serogroup 1/2, despite the very frequent occurrence of this serovar in foodstuffs.

scholarly journals Synergistic hemolysis phenomenon shown by an alpha-toxin-producing Clostridium perfingens and streptococcal CAMP factor in presumptive streptococcal grouping

1978 ◽  
Vol 8 (5) ◽  
pp. 480-488
Author(s):  
S M Gubash
Keyword(s):  
Human Blood ◽  
Test Group ◽  
Blood Agar ◽  
Group A Streptococci ◽  
Alpha Toxin ◽  
Group B Streptococci ◽  
Camp Factor ◽  
Group A ◽  
Camp Test ◽  
Group B

A new phenomenon of synergistic hemolysis by Clostridium perfringens alpha-toxin and the streptococcal CAMP factor on human and guinea pig erythrocytes is described. A possible mode of action of the CAMP factors is suggested. On human blood agar all of the tested isolates of group B streptococci gave an arrowhead-shaped zone of hemolysis; 74% of group A gave a crescent-shaped lytic zone, whereas all isolates of groups C and G and the remaining 26% of group A streptococci gave a bullet-shaped lytic zone. By comparison, in the CAMP test incubated aerobically and anaerobically, 70 and 91%, respectively, of streptococci other than group B gave positive, arrowhead-shaped lytic zones. If all intermediate positive reactions in the CAMP tests were read as negative after aerobic incubation, only 89% of group B streptococci would be properly identified. The synergistic hemolysis phenomenon, using an alpha-toxin-producing C. perfringens and human blood agar, provided a reliable test for presumptive identification of group B streptococci, with promising potential to differentiate in the same test group A streptococci from other groups.

scholarly journals Pig and Goat Blood as Substitutes for Sheep Blood in Blood-Supplemented Agar Media

2000 ◽  
Vol 38 (2) ◽  
pp. 591-594 ◽  
Author(s):  
Chandar Anand ◽  
Rhonda Gordon ◽  
Helene Shaw ◽  
Kevin Fonseca ◽  
Merle Olsen
Keyword(s):  
Anaerobic Bacteria ◽  
Blood Agar ◽  
Anaerobic Conditions ◽  
Sheep Blood ◽  
Wide Range ◽  
Horse Blood ◽  
Agar Media ◽  
Higher Temperature ◽  
Camp Test ◽  
Aerobic And Anaerobic

In many developing countries sheep and horse blood, the recommended blood supplements in bacteriological media, are not readily available, whereas pig and goat blood are. Therefore, this study examined the use of pig and goat blood as potential substitutes for sheep blood in blood-supplemented bacteriologic media commonly used in clinical microbiology laboratories. In general, the growth characteristics and colony morphologies of a wide range of aerobic and anaerobic bacteria and Candida albicans were similar on media containing pig, goat, and sheep blood, although differences were found.Enterococcus sp. uniformly produced alpha-hemolysis when incubated in CO2, but in anaerobic conditions the hemolysis varied. In contrast, beta-hemolytic streptococci produced identical hemolytic reactions on all three media. Synergistic hemolysis was not observed on pig blood agar in the CAMP test nor on goat blood agar in the reverse CAMP test. The preparation of chocolate agar (heated) with pig blood required heating to a higher temperature than with sheep or goat blood to yield suitable growth of Haemophilus species. In general, we conclude that pig and goat blood are suitable alternatives to sheep blood for use in bacteriological media in settings where sheep and horse blood are not readily available.

CAMP test v1

2021 ◽  
Author(s):  
lydiariver not provided
Keyword(s):  
Staphylococcus Aureus ◽  
Streptococcus Agalactiae ◽  
Group B Streptococcus ◽  
Blood Agar ◽  
Camp Factor ◽  
Camp Test ◽  
Group B

Group B Streptococcus agalactiae has CAMP factor which allow it to hemolized zones when it is grown on blood agar plates near to Staphylococcus aureus ATCC 25293 colonies, this effect is brought about by Staphylococcus aureus sphingomyelinase.

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