Crude Necator americanus worm extract diminishes pancreatic islet destruction in diabetic non-obese mice (NOD)

ABSTRACTHygiene hypothesis dictates that the lack of microbial interaction during the first childhood increases the chance of developing autoimmune diseases due to not proper immune system maturation. Helminthes are known by their Th2 and modulatory immune response induction. Here, it was evaluated the influence of Necator americanus antigens during type 1 experimental mouse model (non-obese diabetic – NOD). Intraperitoneal injections for 18 weeks did not impair an inflammatory response, but induced a mixed Th1/Th2 response with presence of IL4 and IL10 from different sources. However, the induced immune response was not sufficient to decrease glucose levels but showed a change in the inflammatory infiltrate in the pancreas. It is necessary more refined studies to clarify the mechanisms of how Necator americanus could impair diabetes progression in mice.

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Taurine Supplementation Regulates Pancreatic Islet Function in Response to Potentiating Agents in Leptin-Deficient Obese Mice

Taurine 9 - Advances in Experimental Medicine and Biology ◽

10.1007/978-3-319-15126-7_28 ◽

2015 ◽

pp. 371-385

Author(s):

Junia Carolina Santos-Silva ◽

Rosane Aparecida Ribeiro ◽

Jean Fraciesco Vettorazzi ◽

Patrícia Cristine Borck ◽

Antonio Carlos Boschero ◽

...

Keyword(s):

Pancreatic Islet ◽

Islet Function ◽

Obese Mice ◽

Taurine Supplementation

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Intermittent fasting benefits on alpha- and beta-cell arrangement in diet-induced obese mice pancreatic islet

Journal of Diabetes and its Complications ◽

10.1016/j.jdiacomp.2019.107497 ◽

2020 ◽

Vol 34 (3) ◽

pp. 107497 ◽

Cited By ~ 1

Author(s):

Thatiany de Souza Marinho ◽

Celina Carvalho Borges ◽

Marcia Barbosa Aguila ◽

Carlos Alberto Mandarim-de-Lacerda

Keyword(s):

Beta Cell ◽

Pancreatic Islet ◽

Intermittent Fasting ◽

Obese Mice ◽

Cell Arrangement

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Pancreatic islet cells disarray, apoptosis, and proliferation in obese mice. The role of Semaglutide treatment

Biochimie ◽

10.1016/j.biochi.2021.10.017 ◽

2021 ◽

Author(s):

Thatiany de Souza Marinho ◽

Fabiane Ferreira Martins ◽

Luiz Eduardo de Macedo Cardoso ◽

Marcia Barbosa Aguila ◽

Carlos Alberto Mandarim-de-Lacerda

Keyword(s):

Pancreatic Islet ◽

Islet Cells ◽

Pancreatic Islet Cells ◽

Obese Mice

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Adverse effects of vitamin D deficiency on the Pi3k/Akt pathway and pancreatic islet morphology in diet-induced obese mice

Molecular Nutrition & Food Research ◽

10.1002/mnfr.201500398 ◽

2015 ◽

Vol 60 (2) ◽

pp. 346-357 ◽

Cited By ~ 12

Author(s):

Celina Carvalho Borges ◽

Andreza Fernandes Salles ◽

Isabele Bringhenti ◽

Vanessa Souza-Mello ◽

Carlos Alberto Mandarim-de-Lacerda ◽

...

Keyword(s):

Vitamin D ◽

Adverse Effects ◽

Vitamin D Deficiency ◽

Pancreatic Islet ◽

Akt Pathway ◽

Obese Mice ◽

Islet Morphology

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Telmisartan, when compared to losartan, shows additional beneficial effects to pancreatic islet structure and function in diet-induced obese mice

Diabetology & Metabolic Syndrome ◽

10.1186/1758-5996-7-s1-a120 ◽

2015 ◽

Vol 7 (Suppl 1) ◽

pp. A120

Author(s):

Francielle Graus-Nunes ◽

Felipe de Olivera Santos ◽

Thatiany de Souza Marinho ◽

D'Angelo Magliano ◽

Fernanda Ornellas ◽

...

Keyword(s):

Pancreatic Islet ◽

Structure And Function ◽

Obese Mice ◽

Beneficial Effects ◽

And Function

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Silver Staining of Pancreatic Islets as Revealed by Electron Microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100060234 ◽

1967 ◽

Vol 25 ◽

pp. 44-45

Author(s):

Kazuaki Misugi ◽

Nobuko Misugi ◽

Hiroshi Yamada

Keyword(s):

Pancreatic Islet ◽

Silver Staining ◽

Islet Cells ◽

Severe Hypoglycemia ◽

Granular Cell ◽

Electron Microscopic Level ◽

Staining Reaction ◽

Electron Microscopic ◽

Pancreatic Islet Cell ◽

D Cell

The authors had described the fine structure of a type of pancreatic islet cell, which appeared different from typical alpha and beta cells, and tentatively considered that this third type of granular cell probably represents the D cell (Figure 1).Since silver staining has been widely used to differentiate different types of pancreatic islet cells by light microscopy, an attempt to examine this staining reaction at the electron microscopic level was made.Material and Method: Surgically removed specimens from three infants who suffered from severe hypoglycemia were used. The specimens were fixed and preserved in 20% neutral formalin. Frozen sections, 30 to 40 micron thick, were prepared and they were stained by Bielschowsky's method as modified by Suzuki (2). The stained sections were examined under a microscope and islet tissues were isolated. They were fixed in 1% osmium tetroxide in phosphate buffer for one hour and embedded in Epon 812 following dehydration through a series of alcohols and propylene oxide.

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