scholarly journals Comparative Study of Serum Gamma Glutamyltransferase, 5’ Nucleotidase and Alkaline Phosphatase in Icteric and an-Icteric Biliary Disease Patients

2013 ◽  
Vol 12 (1) ◽  
Author(s):  
Dr. Maninder Pal Singh Gill
Keyword(s):  
Alkaline Phosphatase ◽  
Significant Rise ◽  
Control Group ◽  
P Value ◽  
Gamma Glutamyl Transferase ◽  
Biliary Disease ◽  
Gamma Glutamyl ◽  
Hepatobiliary Diseases ◽  
Glutamyl Transferase ◽  
Diagnostic Indicator

Introduction: Diagnostic enzymology plays a useful role in evaluation of various hepatobiliary diseases and numerous enzymes have been compared in different disorders. Among these, significance of Gamma Glutamyl Transferase and 5’ Nucleotidase over Alkaline Phosphatase has been stressed repeatedly, but mainly in the icteric obstructive biliary disease patients. In this study, these three enzymes were compared not only in the icteric but also the an-icteric biliary disease patients, particularly to look for elevation and significance of these enzymes in the latter group.Methods: The study was conducted on 50 biliary disease patients, who were further divided into an-icteric (32 patients) and icteric (18 patients) subgroups depending on their bilirubin levels. 50 subjects matched for age and sex with the study group were enrolled for the control group. Gamma Glutamyl Transferase, 5’Nucleotidase, Alkaline Phosphatase and bilirubin levels were evaluated in all the patients as well as the control subjects. Results: All three enzymes showed a significant rise in the icteric subgroup (p value < 0.001). However, in the an-icteric subgroup, only Gamma Glutamyl Transferase and 5’Nucleotidase showed a significant rise. The rise was more for Gamma Glutamyl Transferse (1.60 times normal, p < 0.001) as compared to 5’Nucleotidase (1.39 times normal, p < 0.01). Conclusion: Gamma Glutamyl Transferase and 5’Nucleotidase are useful for evaluation of not only obstructive biliary disease patients but also for the patients with biliary disease who are an-icteric, and out of these two, the former is a more valuable diagnostic indicator in such diseases

scholarly journals Gamma glutamyl transferase and lactate dehydrogenase as biochemical markers of severity of preeclampsia among Sudanese pregnant women

2018 ◽  
Vol 7 (8) ◽  
pp. 3020
Author(s):  
Salih Ezzaldein Salih Elias ◽  
Abdelgadir Eltom ◽  
Ahmed L. Osman ◽  
Asaad MA. Babker
Keyword(s):  
Lactate Dehydrogenase ◽  
Pregnant Women ◽  
Biochemical Markers ◽  
Control Group ◽  
P Value ◽  
Gamma Glutamyl Transferase ◽  
Mean Values ◽  
Gamma Glutamyl ◽  
The Mean ◽  
Glutamyl Transferase

Background: Preeclampsia is a disorder of widespread vascular endothelial malfunction and vasospasm that occurs after 20 weeks' gestation and can present as late as 4-6 weeks post-partum. Pre-eclampsia is a syndrome with multiple aetiologies which has made it difficult to develop adequate screening tests and treatments. Objective of this study to assess the level of gamma glutamyl transferase and lactate dehydrogenase as biochemical markers of severity of preeclampsia in Sudanese pregnant women.Methods: This is a case control hospitalize base study. The study was conducted in preeclamptic pregnant women in Omdurman maternity hospital and medical military hospital in Khartoum state. In this study glutamyl transferase and lactate de-hydrogenase was estimation in 100 Sudanese pregnant women by spectrophotometer method, 50 of them were diagnosed with preeclampsia 27 from this is severe preeclampsia and 23 is mild preeclampsia was matched in aged. Serum samples of all the cases were assayed for GGT and LDH. The data was recorded and analyzed using statistical package for social sciences (SPS –version 16) on programmed computer. The mean standard deviations of variable were calculated for both the test group and the control group and P value for comparison was obtained.Results: The mean values of plasma gamma glutamyl transferase in mild and severe preeclamptic pregnant women were insignificant difference when compared to control P value (0.346 - 0.089) respectively. The mean values of plasma lactate dehydrogenase in mild and severe preeclamptic pregnant women were significant increase when compared to control P value (0.008 - 0.001) respectively.Conclusions: The levels of LDH is raised in Sudanese pregnant women with hypertensive also can be useful biochemical marker that reflects the severity of and the occurrence of complications of pre-eclampsia. GGT levels were normal in Sudanese pregnant women with hypertensive preeclampsia.

scholarly journals Predictor factors for choledocholithiasis

2015 ◽  
Vol 28 (2) ◽  
pp. 109-112 ◽  
Author(s):  
Joana TOZATTI ◽  
André Luiz Parizi MELLO ◽  
Orli FRAZON
Keyword(s):  
Alkaline Phosphatase ◽  
Statistical Significance ◽  
Magnetic Resonance Cholangiopancreatography ◽  
High Specificity ◽  
Control Group ◽  
Interventional Treatment ◽  
Gamma Glutamyl Transferase ◽  
Imaging Studies ◽  
Laboratory Markers ◽  
Glutamyl Transferase

BACKGROUND: The choledocolithiasis has an incidence of 8-20% in patients with cholecystolithiasis. The preoperative diagnosis guides the interventional treatment on the bile duct AIM: To evaluate the sensitivity and specificity of the laboratory markers and imaging studies for choledocholithiasis preoperatively. METHODS: The study comprised 254 patients divided into two groups: the control group (207 patients), patients without choledocholithiasis intraoperatively and cases group (47 patients), that enrolled the patients with choledocholithiasis intra-operatively. Were evaluated the laboratory markers, image exams and intra-operative diagnostic aspects. RESULTS: The sample was homogeneous for age and gender. It was observed that 47% of the cases the patients did not show comorbidities. Hospitalization showes in cases group acute pancreatitis in12.8%, jaundice in 30%, fever in 30% and pain in the right hypochondrium in 95%. By comparing them, was observed that fever and jaundice were the signs and symptoms with statistical significance. Patients with choledocholithiasis had transaminases, alkaline phosphatase, gamma-glutamyl transferase and higher bilirubin with statistical significance (p<0.001). In regard to imaging studies, ultrasound was fairly accurate for cholelithiasis and choledocholithiasis (p<0.001). CONCLUSION: Changes in canalicular and transaminase enzymes are suggestive for preoperative choledocholithiasis; GGT showed better sensitivity and alkaline phosphatase greater specificity; ultrasonography and nuclear magnetic resonance cholangiopancreatography showed high specificity.

scholarly journals Studies on the subcellular localization of human neutrophil alkaline phosphatase

1979 ◽  
Vol 36 (1) ◽  
pp. 401-412
Author(s):  
G.J. Rustin ◽  
P.D. Wilson ◽  
T.J. Peters
Keyword(s):  
Alkaline Phosphatase ◽  
Endoplasmic Reticulum ◽  
Density Gradient ◽  
Human Neutrophil ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Human Neutrophils ◽  
Gamma Glutamyl Transferase ◽  
Gamma Glutamyl ◽  
Glutamyl Transferase

The intracellular localization of alkaline phosphatase has been determined in human neutrophils with analytical subcellular fractionation by density gradient centrifugation and EM cytochemistry. Centrifugation on sucrose gradients containing 1 mM DETA and 5 units/ml of heparin showed that alkaline phosphatase was associated with a membranous component distinct from plasma membrane, mitochondria, specific granules and azurophil granules. There was no resolution from the endoplasmic reticulum. Density gradient centrifugation on a sucrose-imidazole-heparin gradient showed a clear resolution of the alkaline phosphatase-containing membranes from the Golgi and endoplasmic reticulum. Density gradient centrifugation of neutrophils that had been disrupted in the presenceof 0.12 mmol/l. digitonin clearly separated alkaline phosphatase-containing membranes from the endoplasmic reticulum. Part of the gamma-glutamyl transferase has a similar localization to that of alkaline phosphatase. EM cytochemistry of neutrophils, neutrophil homogenates and of the density gradient fractions identified alkaline phosphatase-containing granules as irregular-shaped, often tubular, structures. It is suggested that alkaline phosphatase and part of the gamma-glutamyl transferase activity are localized to a unique organelle in the human neutrophil.

Longitudinal variation in the activities of mucosal enzymes in the small intestine of suckling rats

1996 ◽  
Vol 8 (3) ◽  
pp. 439 ◽  
Author(s):  
RW Lobley ◽  
PC Burrows ◽  
PW Pemberton ◽  
FM Bradbury ◽  
SW D'Souza ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Small Intestine ◽  
Total Activity ◽  
Gamma Glutamyl Transferase ◽  
Suckling Rats ◽  
Distal Intestine ◽  
Gamma Glutamyl ◽  
Rat Pups ◽  
Positional Variation ◽  
Glutamyl Transferase

The extent of positional variation in mucosal enzyme activity along the small intestine was investigated in 14-day-old suckling rats. Samples were taken from ten equally spaced sites along the intestine in 11 rat pups and the activities of the enzymes alkaline phosphatase, neutral aminopeptidase, gamma-glutamyl transferase, lactase and sucrase were measured. All the enzymes except sucrase were subject to considerable positional variation. Alkaline phosphatase and aminopeptidase activities were distributed throughout the intestine, with a broad maximum in the distal intestine. Lactase was also broadly distributed but with greatest activity in the mid intestine. gamma-glutamyl transferase exhibited a novel profile, with a very high proportion of the total activity (78%) present in the distal intestine. Sucrase was essentially absent throughout the intestine.

Sign in / Sign up

Export Citation Format

Share Document