In vitro killing of human glioblastoma by interleukin-2-activated autologous lymphocytes

1986 ◽  
Vol 64 (1) ◽  
pp. 114-117 ◽  
Author(s):  
Steven K. Jacobs ◽  
Debra J. Wilson ◽  
Paul L. Kornblith ◽  
Elizabeth A. Grimm
Keyword(s):  
Tissue Culture ◽  
Brain Tumor ◽  
Tumor Cells ◽  
Peripheral Blood ◽  
Interleukin 2 ◽  
Peripheral Blood Lymphocytes ◽  
Tumor Patients ◽  
Content Type ◽  
Brain Tumor Cells ◽  
Fine Print

✓ Culture of peripheral blood lymphocytes (PBL) from brain-tumor patients with recombinant interleukin-2 (IL-2) results in the activation of lymphokine-activated killer cells (LAK) with the capacity to lyse autologous and allogeneic glioblastoma. In this study, PBL obtained from brain-tumor patients were cultured with or without IL-2 for 3 to 7 days and then tested for their ability to lyse target cells in a 4-hour chromium release cytotoxicity assay. The PBL were drawn 1 to 2 weeks following operative tumor debulking. Cells used as targets included fresh brain-tumor cells obtained at the time of craniotomy, fresh brain-tumor cells grown from 1 to 3 weeks in tissue culture, fresh autologous PBL, and allogeneic glioblastoma cells grown in tissue culture. Peripheral blood lymphocytes from brain-tumor patients that were cultured without IL-2 did not significantly lyse autologous or allogeneic glioblastoma. However, when these PBL were cultured with IL-2, LAK were generated which produced marked lysis of autologous as well as allogeneic tissue-culture glioblastoma in all of eight cases. Significant lysis of autologous fresh tumor by patient LAK was observed in four of five experiments. By contrast, patient LAK did not kill autologous normal PBL. The ability to generate LAK was not influenced by the patient's age, previous therapy, or the administration of steroids.

Does the CO2 laser spread viable brain-tumor cells outside the surgical field?

1984 ◽  
Vol 60 (4) ◽  
pp. 819-820 ◽  
Author(s):  
Rand M. Voorhies ◽  
Michael H. Lavyne ◽  
Timothy A. Strait ◽  
William R. Shapiro
Keyword(s):  
Tissue Culture ◽  
Tumor Cells ◽  
Content Type ◽  
Culture Techniques ◽  
Brain Tumor Cells ◽  
Surgical Field ◽  
Laser Smoke ◽  
Rat Tumor ◽  
Tissue Culture Techniques ◽  
Fine Print

✓ The viability of debris containing C6 rat tumor cells generated by the CO2 laser was investigated using standard tissue culture techniques. No evidence of cell viability was found in the plume of laser smoke.

The use of tissue culture in differentiation between angioblastic meningioma and hemangiopericytoma

1971 ◽  
Vol 34 (3) ◽  
pp. 341-348 ◽  
Author(s):  
Jans Muller ◽  
John Mealey
Keyword(s):  
Tissue Culture ◽  
Tumor Cells ◽  
Cerebellopontine Angle ◽  
Content Type ◽  
Angioblastic Meningioma ◽  
Fine Print

✓ A solid, extrinsic hemangiopericytoma of the cerebellopontine angle was studied histologically and by means of tissue culture. The explanted tumor cells formed classic meningiomatous whorls indicative of the meningeal derivation of this neoplasm. Whorls were entirely absent in the histological preparations, however. The cases reported under the diagnosis of intracranial hemangiopericytoma and angioblastic meningioma have been reviewed; no valid histological distinction between these two types could be made.

Lysis of Autologous Tumor Cells by Peripheral Blood Lymphocytes Treated with Interleukin 2 in Patients with Renal Cell Carcinoma

1987 ◽  
Vol 137 (4) ◽  
pp. 641-647 ◽  
Author(s):  
Etsuji Nakano ◽  
Yasuharu Tada ◽  
Yasuji Ichikawa ◽  
Hideki Fujioka ◽  
Minoru Matsuda ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Tumor Cells ◽  
Peripheral Blood ◽  
Renal Cell ◽  
Interleukin 2 ◽  
Peripheral Blood Lymphocytes ◽  
Autologous Tumor ◽  
Blood Lymphocytes

Assessment of brain tumor cell motility in vivo and in vitro

1995 ◽  
Vol 82 (4) ◽  
pp. 615-622 ◽  
Author(s):  
Michael R. Chicoine ◽  
Daniel L. Silbergeld
Keyword(s):  
Brain Tumor ◽  
Cell Motility ◽  
Human Brain Tumor ◽  
Content Type ◽  
Brain Tumor Cells ◽  
Tumor Cell Motility ◽  
Brain Tumor Cell ◽  
Fine Print

✓ Brain tumor dispersal far from bulk tumor contributes to and, in some instances, dominates disease progression. Three methods were used to characterize brain tumor cell motility in vivo and in vitro: 1) 2 weeks after implantation in rat cerebral cortex, single C6 cells labeled with a fluorescent tag had migrated to brain sites greater than 16 mm distant from bulk tumor; 2) time-lapse videomicroscopy of human brain tumor cells revealed motility of 12.5 µm/hr. Ruffling leading edges and pseudopod formation were most elaborate in more malignant cells; 3) an in vitro assay was devised to quantitatively evaluate motility from a region of high cell density to one of lower cell density. Human brain tumor cells were plated in the center of a petri dish, washed, and refed, establishing a 2-cm circular zone of cells in the dish center. Motility was determined by counting cells daily at predetermined distances from the central zone perimeter. Cells were found 1 cm from the perimeter by 24 hours and 3 cm from the perimeter by 4 days. Increasing serum concentration increased motility; however, neither fibronectin nor arrest of cells in the G0 phase by hydroxyurea altered motility. The addition of cytochalasin B to block cytoskeletal assembly prevented cell motility. Motility increased with increased malignancy. Subpopulations of cells were created by clonal amplification of cells that had migrated most rapidly to the dish periphery. Although morphologically indistinguishable when compared to the original cell line from which they were derived, these subpopulations demonstrated significantly increased motility.

Study of the motility and contractility of cultured brain-tumor cells

1985 ◽  
Vol 62 (6) ◽  
pp. 906-911 ◽  
Author(s):  
Hiroaki Koga
Keyword(s):  
Brain Tumor ◽  
Tumor Cells ◽  
Cultured Cells ◽  
Morphological Changes ◽  
Malignant Cells ◽  
Content Type ◽  
Astrocytoma Cells ◽  
Perfusion Chamber ◽  
Brain Tumor Cells ◽  
Microfilament System

✓ The motility of cultured cells and contractility of their cytoplasmic microfilament system were studied in benign compared with malignant brain-tumor cells. Motility of cultured cells was continuously monitored in a perfusion chamber by a computerized microscope system equipped with an autotracking device. The contractility of the microfilament system was defined by the increase in cell motility when the cell was perfused with an antimicrofilamentous agent, cytochalasin B. The motility and contractility of malignant cells were greater than those of benign cells. The increased contractility of malignant astrocytoma cells was associated with conspicuous morphological changes on electron microscopy. No significant change was observed in the motility, contractility, or morphology in various cells during perfusion with an antimicrotubular agent, colchicine. The significant differences in the motility and contractility of benign compared with malignant cells are believed to originate from qualitative differences of the microfilament system.

Adoptive immunotherapy with peripheral blood lymphocytes cocultured in vitro with autologous tumor cells and interleukin-2

1993 ◽  
Vol 37 (3) ◽  
pp. 175-180 ◽  
Author(s):  
Jonathan R. Sporn ◽  
Michael T. Ergin ◽  
Gerald R. Robbins ◽  
Ritchard G. Cable ◽  
Herbert Silver ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Adoptive Immunotherapy ◽  
Peripheral Blood ◽  
Interleukin 2 ◽  
Peripheral Blood Lymphocytes ◽  
Autologous Tumor ◽  
Blood Lymphocytes

scholarly journals Lysis of fresh autologous tumor cells by interleukin 2 activated peripheral blood lymphocytes in patients with oral cancer.

1989 ◽  
Vol 35 (2) ◽  
pp. 341-360 ◽  
Author(s):  
Yuzo TAKAHASHI ◽  
Yoshiyuki MORI ◽  
Miyuki AZUMA ◽  
Seiji TOMITUKA ◽  
Fujio WAKE ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Tumor Cells ◽  
Peripheral Blood ◽  
Interleukin 2 ◽  
Peripheral Blood Lymphocytes ◽  
Autologous Tumor ◽  
Blood Lymphocytes

Induction of human autologous cytotoxic T lymphocytes against minced tissues of glioblastoma multiforme

1996 ◽  
Vol 84 (2) ◽  
pp. 258-263 ◽  
Author(s):  
Hideo Tsurushima ◽  
Shu Qin Liu ◽  
Koji Tsuboi ◽  
Yoshihiko Yoshii ◽  
Tadao Nose ◽  
...  
Keyword(s):  
Brain Tumor ◽  
Glioblastoma Multiforme ◽  
T Lymphocytes ◽  
Cytotoxic T Lymphocytes ◽  
K562 Cells ◽  
Peripheral Blood Lymphocytes ◽  
Autologous Plasma ◽  
Content Type ◽  
Brain Tumor Cells ◽  
The Brain

✓ The authors induced autologous cytotoxic T lymphocytes (CTLs) directly from peripheral blood lymphocytes by preparing a coculture of minced tissue fragments of glioblastoma multiforme (GBM) with interleukins-1, -2, -4, and -6 and interferon-g in RHAMa medium containing 5% autologous plasma for 2 weeks. At the end of this period, the frequencies of CD3+, CD4+, CD8+, and CD16+ lymphocytes were 95% to 99%, 40% to 62%, 37% to 38%, and 0.2%, respectively. The lymphocytes killed 82% to 100% of the GBM cells within 48 hours at an effector-to-target cell ratio of 1.67, whereas in a separate coculture, autologous lymphokine-activated killer (LAK) cells killed only 33% of GBM cells under the same conditions. The lymphocytes showed no cytotoxicity against LAK-sensitive Daudi cells, natural killer—sensitive K562 cells or autologous fibroblasts grown from the brain tumor, although they did show slight cytotoxicities against allogeneic GBM cell lines. These results lead the authors to suggest that the lymphocyte population contains specific CTLs for autologous brain tumor cells and that these CTLs could be effective in adoptive immunotherapy to combat brain tumor.

scholarly journals Detection of Brain Tumor Cells in the Peripheral Blood by a Telomerase Promoter-Based Assay

2014 ◽  
Vol 74 (8) ◽  
pp. 2152-2159 ◽  
Author(s):  
Kelly M. MacArthur ◽  
Gary D. Kao ◽  
Sanjay Chandrasekaran ◽  
Michelle Alonso-Basanta ◽  
Christina Chapman ◽  
...  
Keyword(s):  
Brain Tumor ◽  
Tumor Cells ◽  
Peripheral Blood ◽  
Brain Tumor Cells
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