Morphological and molecular characterization of teosinte derived maize population

2020 ◽  
Vol 79 (04) ◽  
Author(s):  
Amarjeet Kumar ◽  
N. K. Singh ◽  
Sneha Adhikari ◽  
Anjali Joshi
Keyword(s):  
Molecular Characterization ◽  
Quantitative Traits ◽  
Morphological Characterization ◽  
Linkage Groups ◽  
Germplasm Enhancement ◽  
Maize Germplasm ◽  
Maize Population ◽  
Quantitative Characters ◽  
Morphological And Molecular Characterization

Germplasm enhancement seems to be an essential part of a breeding programme to improve resiliency, adaptability and productivity of the crops. To develop and diversify the maize germplasm, teosinte a wild relative of maize was integrated in crossing programme and BC1F4 lines were developed. Five BC1F4 lines along with teosinte and maize inbred DI-103 were characterised using quantitative characters and molecular markers. Morphological characterization was done with the help of visual parameters and quantitative traits and for molecular characterization fifty six SSR markers were used. SSR data were analysed with the help of software Mapmaker and twelve linkage groups were generated. Maximum allelic contributions from parent teosinte were found in the introgressed line AM-5 (53.4%) followed by AM-12 (48.9 %), whereas, least contribution of 34.1 % was found in AM-7. The maximum genetic distance among the introgressed lines was observed between AM-2 and AM-9 (0.75) followed by AM-2 and AM-7 (0.70), AM-7 and AM-9 (0.70). The maximum number of cob was found in AM-5 (5.00) followed by AM-2 (4.00). Grain yield per plant was found highest for AM-2 (100.00 g) followed by AM-12 (80.00 g), while, least value was observed for AM-7 (42.00 g). The results indicated differential parental contributions which leads to diversification in the progenies derived from diverse crosses in maize and further opined that such crosses seems to be essential for creating adapted germplasm to whom breeders are looking for.

scholarly journals Bazı Yerli ve Yabancı Trabzon Hurması (Diospyros kaki L.) Çeşit ve Tiplerinin Morfolojik ve Moleküler Karakterizasyonu

2017 ◽  
Vol 5 (12) ◽  
pp. 1580
Author(s):  
Turgut Yeşiloğlu ◽  
Yıldız Aka Kacar ◽  
Bilge Yılmaz ◽  
Meral İncesu ◽  
Berken Çimen
Keyword(s):  
Molecular Characterization ◽  
Rapd Markers ◽  
Genetic Relationships ◽  
Similarity Index ◽  
Development Stage ◽  
Morphological Characterization ◽  
Diospyros Kaki ◽  
High Level ◽  
Morphological And Molecular Characterization

In this study, carried out in University of Cukurova, Faculty of Agriculture, Department of Horticulture, morphological and molecular characterization of 20 persimmon accessions were performed. To determine morphological characterization, plant development stage, growth, and brunch structure, characteristics of leaf, fruit and seed were investigated. Data obtained by morphological characterization were subjected to similarity coefficient, principal components and cluster analyses to demonstrate the overall phenotypic relationships among these genotypes. The first 3 components contained 55.11% of the total multivariate variation. According to a cluster analysis, the similarity index of the population consists of 20 persimmon genotypes ranged between 6.01% and 67.47%. According to molecular characterization by full name (RAPD) markers, high level of genetic polymorphism was determined in persimmons. Although the high polymorphism, close genetic relationships have been determined among some genotypes. According to the RAPD markers, 82% similarity level was identified between ‘Hana Fuyu’ (Fr) and ‘Hachiya’ (Fr). Also ‘Fuji’ variety has shown a similarity of 80% between these genotypes. In addition, ‘Kaki Tipo’ (Fr), 07 TH 05, TH 01 07 TH 06 and 33 genotypes were clustered in the same group, and similarity level has been determined to be above 65%.

scholarly journals Collection and agro morphological characterization of Algerian accessions of lentil (Lens culinaris)

2018 ◽  
Vol 19 (1) ◽  
pp. 183-193 ◽  
Author(s):  
DJOUHER GAAD ◽  
MERIEM LAOUAR ◽  
AISSA ABDELGUERFI ◽  
FATIMA GABOUN
Keyword(s):  
Lens Culinaris ◽  
Quantitative Traits ◽  
Hierarchical Cluster ◽  
Morphological Characterization ◽  
Ecological Zones ◽  
Morphological Evaluation ◽  
Quantitative Characters ◽  
Arid Conditions ◽  
Agro Ecological Zones

Gaad D, Laouar M, Gaboun F, Abdelguerfi A. 2018. Collection and agro morphological characterization of Algerian accessions of lentil (Lens culinaris). Biodiversitas 19: 183-193. Lentil is one of the important pulse crops in Algeria. The narrow genetic base of local cultivars and the disappearance of many local accessions contribute to the loss of lentil biodiversity. Therefore, collection, characterization and preservation of existing local accessions of lentils are important. Lentil accessions were collected across different agro-ecological zones of Algeria. From 10 regions (Departments), 15 villages were surveyed and 30 accessions were collected. Eighteen local accessions and 26 references collection have been used for agro morphological evaluation. The assessment was performed in two locations, sub-humid and semi-arid conditions, based on 12 quantitative characters. The result of variance analysis shows a significant effect of the interaction genotype x location for six quantitative traits. Three macrosperma Algerian accessions characterized by a high number of pod/plant, number of seed/plant and seed yield/plant were positioned on PCA1. The remaining Algerian accessions were positioned on PCA2 and were characterized by later flowering and maturity with high plant stature and height of the lowest pods. Hierarchical cluster analysis grouped the accessions into five distinct clusters independently of the accessions origins and revealed the distribution of Algerian accessions in all of the groups. This study will help breeders to better select accessions to be used in lentil breeding program.

scholarly journals Reliability of morphological and molecular characterization of lightsprouts for differentiation of potato accessions

Genetika ◽  
2016 ◽  
Vol 48 (2) ◽  
pp. 525-532
Author(s):  
Peter Dolnicar ◽  
Drago Milosevic ◽  
Zoran Jovovic ◽  
Vladimir Meglic ◽  
Marko Maras ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Molecular Characterization ◽  
Morphological Characterization ◽  
Gene Bank ◽  
Simple Method ◽  
Rapid Procedure ◽  
Molecular Assessment ◽  
Biotechnical Faculty ◽  
Morphological And Molecular Characterization

The study of reliability of morphological characterization of lightsprouts for differentiation of potato varieties was performed at the Agricultural Institute of Slovenia in cooperation with Biotechnical Faculty Podgorica in order to introduce simple method for further characterization of potato accessions in Montenegrin gene bank. Seven selected, potentially different, potato accessions preserved in the Montenegrin gene bank were used for morphological characterization of lightsprouts. Using UPOV guidelines 11 lightsprout traits were estimated. Molecular assessment was carried out in parallel with morphological characterization by six microsatellite (SSR) markers. The latter successfully distinguished all accessions but two, while four different lightsprout phenotypes were identified in morphological characterization. Though molecular markers showed more strength in resolving relationships between genotypes, characterization of lightsprouts still demonstrated its usefulness due to cheap, simple and rapid procedure.

Characterization of Antibacterial-Producing Endophytic Fungi of Syzygiumpolyanthum Leaves

2020 ◽  
Vol 20 (4) ◽  
pp. 448-454
Author(s):  
Rahmita Burhamzah ◽  
Gemini Alam ◽  
Herlina Rante
Keyword(s):  
Molecular Characterization ◽  
Endophytic Fungi ◽  
Morphological Characterization ◽  
Test Method ◽  
Analysis Method ◽  
Nitrogen Base ◽  
Rdna Genes ◽  
Antibacterial Screening ◽  
Planting Method

Background: Endophytic fungi live in plants’ tissue and can produce the same bioactive compounds as its host plant produces. Syzygiumpolyanthum leaves have known to be one of the antibacterial compound producers. Aim and Objective: This study aimed to characterize morphologically, microscopically, and molecularly the antibacterial-producing endophytic fungi of Syzygiumpolyanthum leaves. Methods: The isolation of endophytic fungi was done by fragment planting method on PDA medium. The antibacterial screening was performed using the antagonistic test as the first screening followed by the disc diffusion test method. The morphological characterization was based on isolate’s mycelia color, growth pattern, margin, and surface texture of the colony, while the microscopic characterization was based on its hyphae characteristics. The molecular characterization of the isolate was done by nitrogen base sequence analysis method on nucleotide constituent of ITS rDNA genes of the isolate. Results: The results found that isolate DF1 has antibacterial activity against E.coli, S.aureus, P.acne, and P.aeruginosa, with the greatest inhibition at 10% concentration of broth fermentation extract on S.aureus with a diameter of inhibition of 13.77 mm. Conclusion: Based on macroscopic, microscopic, and molecular characterization, DF1 isolate is similar to Ceriporialacerate.

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