scholarly journals Expression of Zinc Finger Protein Zat12 from Arabidopsis thaliana in Escherichia coli

2020 ◽  
Vol 3 (1) ◽  
pp. 504-511
Author(s):  
Le Thi Tuyet Cham ◽  
Vu Ngoc Thang ◽  
Tran Anh Tuan ◽  
Vu Thi Thuy Hang
Keyword(s):  
Arabidopsis Thaliana ◽  
Zinc Finger ◽  
Stress Responses ◽  
Zinc Finger Protein ◽  
Expression System ◽  
Direct Interaction ◽  
Early Response ◽  
In Planta ◽  
Abiotic Stress Response ◽  
Finger Protein

The C2H2 zinc finger protein ZAT12 has been classified as a plant core abiotic stress response gene in the early  response to multiple stresses. ZAT12 links the iron deficiency and oxidative stress responses through the direct interaction with/and negative regulation of a central regulator - FIT. For further research on the regulation of the ZAT12 protein in planta, a huge quantity of ZAT12 proteins is required to inject into mice for the generation of ZAT12 antiserum. In this study, the gene encoding the ZAT12 protein from Arabidopsis thaliana was cloned into the expression vector - pETBlue-2 and then overexpressed in E. coli T7. A high expression level was indicated by SDS-PAGE. Immunoblot demonstrated successful expression using a bacterial expression system.

The CCCH zinc finger protein gene AtZFP1 improves salt resistance in Arabidopsis thaliana

2014 ◽  
Vol 86 (3) ◽  
pp. 237-253 ◽  
Author(s):  
Guoliang Han ◽  
Mingjie Wang ◽  
Fang Yuan ◽  
Na Sui ◽  
Jie Song ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Zinc Finger ◽  
Protein Gene ◽  
Zinc Finger Protein ◽  
Salt Resistance ◽  
Zinc Finger Protein Gene ◽  
Ccch Zinc Finger ◽  
Finger Protein

scholarly journals Effect of His-Tag on Expression, Purification, and Structure of Zinc Finger Protein, ZNF191(243-368)

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Dongxin Zhao ◽  
Zhongxian Huang
Keyword(s):  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Expression System ◽  
Zinc Finger Proteins ◽  
Hereditary Diseases ◽  
Expression Systems ◽  
His Tag ◽  
Finger Protein ◽  
Inclusion Body Formation ◽  
Structure And Functions

Zinc finger proteins are associated with hereditary diseases and cancers. To obtain an adequate amount of zinc finger proteins for studying their properties, structure, and functions, many protein expression systems are used. ZNF191(243-368) is a zinc finger protein and can be fused with His-tag to generate fusion proteins such as His6-ZNF191(243-368) and ZNF191(243-368)-His8. The purification of His-tag protein using Ni-NTA resin can overcome the difficulty of ZNF191(243-368) separation caused by inclusion body formation. The influences of His-tag on ZNF191(243-368) properties and structure were investigated using spectrographic techniques and hydrolase experiment. Our findings suggest that insertion of a His-tag at the N-terminal or C-terminal end of ZNF191(243-368) has different effects on the protein. Therefore, an expression system should be considered based on the properties and structure of the protein. Furthermore, the hydrolase activity of ZNF191(243-368)-His8has provided new insights into the design of biological functional molecules.

A putative maize zinc-finger protein gene, ZmAN13, participates in abiotic stress response

2011 ◽  
Vol 107 (1) ◽  
pp. 101-112 ◽  
Author(s):  
Ning Xuan ◽  
Ying Jin ◽  
Hongwei Zhang ◽  
Yuanhong Xie ◽  
Yunjun Liu ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Response ◽  
Zinc Finger ◽  
Protein Gene ◽  
Zinc Finger Protein ◽  
Abiotic Stress Response ◽  
Zinc Finger Protein Gene ◽  
Finger Protein

The zinc finger protein ZAT11 modulates paraquat-induced programmed cell death in Arabidopsis thaliana

2013 ◽  
Vol 35 (6) ◽  
pp. 1863-1871 ◽  
Author(s):  
Muhammad Kamran Qureshi ◽  
Neerakkal Sujeeth ◽  
Tsanko S. Gechev ◽  
Jacques Hille
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Death ◽  
Programmed Cell Death ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein

scholarly journals The Barley Stripe Mosaic Virus expression system reveals the wheat C2H2 zinc finger protein TaZFP1B as a key regulator of drought tolerance

2019 ◽  
Author(s):  
Mario Houde ◽  
Arnaud Cheuk ◽  
François Ouellet
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Mosaic Virus ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Expression System ◽  
Functional Characterization ◽  
Barley Stripe Mosaic Virus ◽  
Finger Protein

Abstract Drought stress is one of the major factors limiting wheat production globally. Improving drought tolerance is important for agriculture sustainability. Although various morphological, physiological and biochemical responses associated with drought tolerance have been documented, the molecular mechanisms and regulatory genes that are needed to improve drought tolerance in crops require further investigation. We have used a novel 4-component version (for overexpression) and a 3-component version (for underexpression) of a barley stripe mosaic virus-based (BSMV) system for functional characterization of the C2H2-type zinc finger protein TaZFP1B in wheat. These expression systems avoid the need to produce transgenic plant lines and greatly speeds up functional gene characterization.Results We show that overexpression of TaZFP1B stimulates plant growth and up-regulates different oxidative stress-responsive genes under well-watered conditions. Plants that overexpress TaZFP1B are more drought tolerant at critical periods of the plant’s life cycle. Furthermore, RNA-Seq analysis revealed that plants overexpressing TaZFP1B reprogram their transcriptome, resulting in physiological and physical modifications that help wheat to grow and survive under drought stress. In contrast, plants transformed to underexpress TaZFP1B are significantly less tolerant to drought and growth is negatively affected.Conclusions This study clearly shows that the two versions of the BSMV system can be used for fast and efficient functional characterization of genes in crops. The extent of transcriptome reprogramming in plants that overexpress TaZFP1B indicates that the encoded transcription factor is a key regulator of drought tolerance in wheat.

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