scholarly journals Ectopic expression of GmZAT4, a putative C2H2-type zinc finger protein, enhances PEG and NaCl stress tolerances in Arabidopsis thaliana

3 Biotech ◽  
2019 ◽  
Vol 9 (5) ◽  
Author(s):  
Zhaoxia Sun ◽  
Ronghua Liu ◽  
Bin Guo ◽  
Kesheng Huang ◽  
Li Wang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Ectopic Expression ◽  
Nacl Stress ◽  
Finger Protein

The CCCH zinc finger protein gene AtZFP1 improves salt resistance in Arabidopsis thaliana

2014 ◽  
Vol 86 (3) ◽  
pp. 237-253 ◽  
Author(s):  
Guoliang Han ◽  
Mingjie Wang ◽  
Fang Yuan ◽  
Na Sui ◽  
Jie Song ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Zinc Finger ◽  
Protein Gene ◽  
Zinc Finger Protein ◽  
Salt Resistance ◽  
Zinc Finger Protein Gene ◽  
Ccch Zinc Finger ◽  
Finger Protein

scholarly journals RABBIT EARS , encoding a SUPERMAN-like zinc finger protein, regulates petal development in Arabidopsis thaliana

Development ◽  
2011 ◽  
Vol 138 (16) ◽  
pp. 3591-3591 ◽  
Author(s):  
Seiji Takeda ◽  
Noritaka Matsumoto ◽  
Kiyotaka Okada
Keyword(s):  
Arabidopsis Thaliana ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein ◽  
Petal Development ◽  
Rabbit Ears

scholarly journals Expression of Zinc Finger Protein Zat12 from Arabidopsis thaliana in Escherichia coli

2020 ◽  
Vol 3 (1) ◽  
pp. 504-511
Author(s):  
Le Thi Tuyet Cham ◽  
Vu Ngoc Thang ◽  
Tran Anh Tuan ◽  
Vu Thi Thuy Hang
Keyword(s):  
Arabidopsis Thaliana ◽  
Zinc Finger ◽  
Stress Responses ◽  
Zinc Finger Protein ◽  
Expression System ◽  
Direct Interaction ◽  
Early Response ◽  
In Planta ◽  
Abiotic Stress Response ◽  
Finger Protein

The C2H2 zinc finger protein ZAT12 has been classified as a plant core abiotic stress response gene in the early  response to multiple stresses. ZAT12 links the iron deficiency and oxidative stress responses through the direct interaction with/and negative regulation of a central regulator - FIT. For further research on the regulation of the ZAT12 protein in planta, a huge quantity of ZAT12 proteins is required to inject into mice for the generation of ZAT12 antiserum. In this study, the gene encoding the ZAT12 protein from Arabidopsis thaliana was cloned into the expression vector - pETBlue-2 and then overexpressed in E. coli T7. A high expression level was indicated by SDS-PAGE. Immunoblot demonstrated successful expression using a bacterial expression system.

The zinc finger protein ZAT11 modulates paraquat-induced programmed cell death in Arabidopsis thaliana

2013 ◽  
Vol 35 (6) ◽  
pp. 1863-1871 ◽  
Author(s):  
Muhammad Kamran Qureshi ◽  
Neerakkal Sujeeth ◽  
Tsanko S. Gechev ◽  
Jacques Hille
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Death ◽  
Programmed Cell Death ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein

scholarly journals Redox-Dependent Regulation of the Stress-Induced Zinc-Finger Protein SAP12 in Arabidopsis thaliana

2009 ◽  
Vol 2 (2) ◽  
pp. 357-367 ◽  
Author(s):  
Elke Ströher ◽  
Xin-Jia Wang ◽  
Nils Roloff ◽  
Peter Klein ◽  
Arne Husemann ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein

scholarly journals The tumor suppressor Zinc finger protein 471 suppresses breast cancer growth and metastasis through inhibiting AKT and Wnt/β-catenin signaling

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Chunfang Tao ◽  
Juan Luo ◽  
Jun Tang ◽  
Danfeng Zhou ◽  
Shujun Feng ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Tumor Suppressor ◽  
Tumor Cell ◽  
Zinc Finger ◽  
Molecular Mechanisms ◽  
Zinc Finger Protein ◽  
Ectopic Expression ◽  
Surgical Margin ◽  
Mesenchymal Transition ◽  
Finger Protein

Abstract Background Zinc-finger protein 471 (ZNF471) is a member of the Krüppel-associated box domain zinc finger protein (KRAB-ZFP) family. ZNF471 is methylated in squamous cell carcinomas of tongue, stomach and esophageal. However, its role in breast carcinogenesis remains elusive. Here, we studied its expression, functions, and molecular mechanisms in breast cancer. Methods We examined ZNF471 expression by RT-PCR and qPCR. Methylation-specific PCR determined its promoter methylation. Its biological functions and related molecular mechanisms were assessed by CCK-8, clonogenicity, wound healing, Transwell, nude mice tumorigenicity, flow cytometry, BrdU-ELISA, immunohistochemistry and Western blot assays. Results ZNF471 was significantly downregulated in breast cell lines and tissues due to its promoter CpG methylation, compared with normal mammary epithelial cells and paired surgical-margin tissues. Ectopic expression of ZNF471 substantially inhibited breast tumor cell growth in vitro and in vivo, arrested cell cycle at S phase, and promoted cell apoptosis, as well as suppressed metastasis. Further knockdown of ZNF471 verified its tumor-suppressive effects. We also found that ZNF471 exerted its tumor-suppressive functions through suppressing epithelial-mesenchymal transition, tumor cell stemness and AKT and Wnt/β-catenin signaling. Conclusions ZNF471 functions as a tumor suppressor that was epigenetically inactivated in breast cancer. Its inhibition of AKT and Wnt/β-catenin signaling pathways is one of the mechanisms underlying its anti-cancer effects.

Drumstick is a zinc finger protein that antagonizes Lines to control patterning and morphogenesis of theDrosophilahindgut

Development ◽  
2002 ◽  
Vol 129 (15) ◽  
pp. 3645-3656 ◽  
Author(s):  
Ryan B. Green ◽  
Victor Hatini ◽  
Katherine A. Johansen ◽  
Xue-Jun Liu ◽  
Judith A. Lengyel
Keyword(s):  
Gene Expression ◽  
Small Intestine ◽  
Amino Acid ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Ectopic Expression ◽  
Genetic Data ◽  
Specific Pattern ◽  
Cell Rearrangement ◽  
Finger Protein

Elongation of the Drosophila embryonic hindgut epithelium occurs by a process of oriented cell rearrangement requiring the genes drumstick (drm) and lines (lin). The elongating hindgut becomes subdivided into domains – small intestine, large intestine and rectum – each characterized by a specific pattern of gene expression dependent upon normal drm and lin function. We show that drm encodes an 81 amino acid (10 kDa) zinc finger protein that is a member of the Odd-skipped family. drm expression is localized to the developing midgut-hindgut junction and is required to establish the small intestine, while lin is broadly expressed throughout the gut primordium and represses small intestine fate. lin is epistatic to drm, suggesting a model in which localized expression of drm blocks lin activity, thereby allowing small intestine fate to be established. Further supporting this model, ectopic expression of Drm throughout the hindgut produces a lin phenotype. Biochemical and genetic data indicate that the first conserved zinc finger of Drm is essential for its function. We have thus defined a pathway in which a spatially localized zinc finger protein antagonizes a globally expressed protein, thereby leading to specification of a domain (the small intestine) necessary for oriented cell rearrangement.

Hedgehog activity, independent of decapentaplegic, participates in wing disc patterning

Development ◽  
1997 ◽  
Vol 124 (6) ◽  
pp. 1227-1237 ◽  
Author(s):  
J.L. Mullor ◽  
M. Calleja ◽  
J. Capdevila ◽  
I. Guerrero
Keyword(s):  
Zinc Finger ◽  
Imaginal Disc ◽  
Zinc Finger Protein ◽  
Ectopic Expression ◽  
Wing Disc ◽  
Signal Molecule ◽  
Cubitus Interruptus ◽  
Drosophila Wing ◽  
Finger Protein

In the Drosophila wing imaginal disc, the Hedgehog (Hh) signal molecule induces the expression of decapentaplegic (dpp) in a band of cells abutting the anteroposterior (A/P) compartment border. It has been proposed that Dpp organizes the patterning of the entire wing disc. We have tested this proposal by studying the response to distinct levels of ectopic expression of Hh and Dpp, using the sensory organ precursors (SOPs) of the wing and notum and the presumptive wing veins as positional markers. Here, we show that Dpp specifies the position of most SOPs in the notum and of some of them in the wing. Close to the A/P compartment border, however, SOPs are specified by Hh rather than by Dpp alone. We also show that late signaling by Hh, after setting up dpp expression, is responsible for the formation of vein 3 and the scutellar region, and also for the determination of the distance between veins 3 and 4. One of the genes that mediates the Hh signal is the zinc-finger protein Cubitus interruptus (Ci). These results indicate that Hh has a Dpp-independent morphogenetic effect in the region of the wing disc near the A/P border.

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