Molecular Identification of Fusarium Brachygibbosum And Some Isolates of Trichoderma Spp.

Abstract. Lutfia A, Munir E, Yurnaliza. 2020. Molecular identification of endophytic fungi from torch ginger (Etlingera elatior) antagonist to phytopathogenic fungi. Biodiversitas 21: 2681-2689. Medicinal plants are valuable sources in exploration of endophytic microorganisms. The utilization of culturable endophytes, particularly endophytic fungi in pharmacological aspects has been reported in many literature. Zingiberaceae, being known as one of medicinal plant families, is commonly used in tropical countries, e.g Indonesia to treat health disorders and ailments. However, little information has been reported upon their associative endophytic fungi that may be used as biocontrol agents against phytopathogenic fungi. Efforts to elaborate on the symbiotic relationship between endophytic fungi and host to explain the antifungal ability from endophytes are presented in this study. Six fungal morphotypes were recovered from the rhizome fragment of torch ginger (Etlingera elatior (Jack) RM Smith) sampled from Sibayak forest, North Sumatra, Indonesia. All isolates displayed potential antifungal activities against Fusarium oxysporum, Ganoderma boninense, and Rigidoporus lignosus as representative phytopathogens in a dual culture plate assay. All fungal morphotypes were identified based on their 18S rDNA-ITS regions. Molecular identification revealed that most isolates were related to Trichoderma spp. while one isolate was identified as Pestaliotopsis sp. The endophytic assemblage of mostly Trichoderma strains in our study may reveal a mutual relationship of these soil fungi as root colonizer displaying biocontrol properties against phytopathogenic fungi to host.

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Molecular Identification of Rhizosphere Trichoderma spp. and Their Antagonistic Impact Against Some Plant Pathogenic Fungi

Baghdad Science Journal ◽

10.21123/bsj.2016.13.1.0053 ◽

2016 ◽

Vol 13 (1) ◽

pp. 53-65

Author(s):

Safaa Al-qaysi ◽

◽

Adel Alwan ◽

Keyword(s):

Molecular Identification ◽

Pathogenic Fungi ◽

Plant Pathogenic Fungi ◽

Trichoderma Spp

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Molecular Identification, Enzyme Assay, and Metabolic Profiling of Trichoderma spp.

Journal of Microbiology and Biotechnology ◽

10.4014/jmb.1702.02063 ◽

2017 ◽

Vol 27 (6) ◽

pp. 1157-1162

Author(s):

Soo-Jung Bae ◽

Young-Hwan Park ◽

Hyeun-Jong Bae ◽

Junhyun Jeon ◽

Hanhong Bae

Keyword(s):

Molecular Identification ◽

Metabolic Profiling ◽

Enzyme Assay ◽

Trichoderma Spp

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Molecular Identification of Trichoderma spp. in Garlic and Onion Fields and In Vitro Antagonism Trials on Sclerotium cepivorum

Revista Brasileira de Ciência do Solo ◽

10.1590/18069657rbcs20150454 ◽

2016 ◽

Vol 40 (0) ◽

Author(s):

Luis Alvarado-Marchena and ◽

William Rivera-Méndez

Keyword(s):

Molecular Identification ◽

Trichoderma Spp ◽

Sclerotium Cepivorum

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Molecular Identification of Rhizosphere Trichoderma spp. and Their Antagonistic Impact Against Some Plant Pathogenic Fungi

Baghdad Science Journal ◽

10.21123/bsj.13.1.53-65 ◽

2016 ◽

Vol 13 (1) ◽

pp. 53-65

Author(s):

Baghdad Science Journal

Keyword(s):

Molecular Identification ◽

Dna Sequences ◽

Magnaporthe Grisea ◽

Genetic Relationships ◽

Its Region ◽

Inhibitory Effect ◽

Phytopathogenic Fungi ◽

Trichoderma Spp ◽

Region Sequence ◽

Trichoderma Isolates

The main aim of this study was to molecular identification and determine the antagonistic impact of rhizosphere Trichoderma spp. against some phytopathogenic fungi, including (Magnaporthe grisea) pyricularia oryzae, Rhizoctonia solani and Macrophomina phasolina. Four Trichoderma isolates were isolated from rhizosphere soils of the different host plants in different locations of Egyptian governorates. The morphological characterization of isolated Trichoderma as well as using of (ITS1-5.8S-ITS2) ribosomal gene sequence acquisition and data analyses. By comparing the results of DNA sequences of ITS region, the fungi represented one isolate were positively identified as T. asperellum (1 isolate T1) and one as T. longibrachiatum (1 isolate T2) and two as Trichoderma harzianum (2 isolates T3 and T4). The results showed similarity value of (5.8S-ITS) region sequence of the two isolates, T1 (T. asperellum) and T2 (T. longibrachiatum) of (99%, 99%), respectively. The similarity value of (5.8S-ITS) region sequence with isolates of T3, T4 (T. harzianum) of (99%). On the other side, the results of molecular identification of phytopathogenic fungi represented high similarity value of (5.8S-ITS) region sequence and were identified as P.oryzae, R. solani and M. phasolina (99, 96 and 99%) respectively. Variations and genetic relationships among 4 Trichoderma isolates were investigated by using the Rapid Amplification of Polymorphic DNA (RAPD) profiles using ten random primers. All Trichoderma isolates were assessed for their antagonistic impact on phytopathogens P. oryzae, R. solani and M. phasolina. Though T. harzianum isolates were more affects than T. longibrachiatum and T. asperellum isolates, the percent inhibitory effect among T. harzianum isolates were vary much (44.8 to 91.6%). The inhibitory effect of T. asperellum isolates ranged from 42.2 to (86.0%), while T. longibrachiatum exhibiting affect ranged between (47.5%) to (83.8%).

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Electron microscopy in diagnostic virology

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100110702 ◽

1984 ◽

Vol 42 ◽

pp. 70-73

Author(s):

S. E. Miller

Keyword(s):

Electron Microscopy ◽

Tissue Culture ◽

Molecular Identification ◽

Point Of View ◽

Negative Stain ◽

Viral Diagnosis ◽

Electron Microscopist ◽

Diagnostic Virology

The techniques for detecting viruses are many and varied including FAT, ELISA, SPIRA, RPHA, SRH, TIA, ID, IEOP, GC (1); CF, CIE (2); Tzanck (3); EM, IEM (4); and molecular identification (5). This paper will deal with viral diagnosis by electron microscopy and will be organized from the point of view of the electron microscopist who is asked to look for an unknown agent--a consideration of the specimen and possible agents rather than from a virologist's view of comparing all the different viruses. The first step is to ascertain the specimen source and select the method of preparation, e. g. negative stain or embedment, and whether the sample should be precleared by centrifugation, concentrated, or inoculated into tissue culture. Also, knowing the type of specimen and patient symptoms will lend suggestions of possible agents and eliminate some viruses, e. g. Rotavirus will not be seen in brain, nor Rabies in stool, but preconceived notions should not prejudice the observer into missing an unlikely pathogen.

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