Estimation of Total Phenolic, Total Flavonoid Content and Assessment of in vitro Antioxidant Activity of Extracts of plant Maytenus emarginata stembark

Author(s):  
Kalpana S ◽  
Ramakrushna B. ◽  
Anitha S.

Objective: The present study evaluates the antioxidant and α-amylase inhibitory activity of leaf and bark extracts of Phyllanthus indofischeri with methanol and water as solvents. In addition to this, the total phenolic content and total flavonoid content was determined.Methods: The total phenolic and total flavonoid content of the extracts was determined by folin ciocaletus reagent method and aluminium chloride colorimetric method respectively. The antioxidant and α-amylase inhibitory activity were measured by various assays, including α, α-diphenyl-ẞ-dipicryl-hydrazyl (DPPH) free radical scavenging, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonicacid) (ABTS) radical scavenging, superoxide radical scavenging, total antioxidant capacity by phosphomolybdate method and porcine pancreatic α-amylase inhibitory assay. The IC50 values were calculated and compared with standards such as gallic acid, ascorbic acid and α-acarbose.Results: The results illustrated that all the extracts of Phyllanthus indofischeri exhibit significant antioxidant and α-amylase inhibitory activity. Among the extracts, methanolic leaf extract showed high levels of activity followed by bark water extract.Conclusion: Phyllanthus indofischeri extracts had shown antioxidant and α-amylase inhibitory activity. On the basis of these results, Phyllanthus indofischeri can be used as a natural antioxidant and hypoglycemic agent against various disorders related to oxidative stress; and the isolation of bioactive compounds was warranted. 


2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Thandiwe Alide ◽  
Phanice Wangila ◽  
Ambrose Kiprop

Abstract Objective To investigate the effect of cooking temperature and time on the total phenolic content, total flavonoid content and antioxidant activity of aqueous and ethanolic extracts of garlic. Results The mean total phenolic content of fresh garlic were 303.07 ± 6.58 mg gallic acid equivalent per 100 g (GAE/100 g) and 638.96 ± 15.30 mg GAE/100 g of plant material for the aqueous and ethanolic extracts respectively. The mean total flavonoid content 109.78 ± 6.78 mg quercetin equivalent per 100 g (QE/100 g) and 258.47 ± 12.37 QE/100 g for aqueous and ethanolic extracts respectively. Fourier transform infrared spectral data showed absorptions in the range for carboxylic acids, hydroxyl group, esters, and alcohols, confirming the presence of phenols and flavonoids in the extracts. Cooking temperature had a significant effect on total phenolic content and total flavonoid content while cooking time did not have a significant effect on the phytochemicals and antioxidant activity.


2020 ◽  
Vol 19 (1) ◽  
pp. 61-70
Author(s):  
Erdal Ağlar ◽  
Ahmet Sümbül ◽  
Orhan Karakaya ◽  
Burhan Ozturk

The study was conducted in 2017 in the district of Sivas. In the study, 20 genotypes, which are considered to be different from each other, taking into account the fruit characteristics such as color and size and shape, were determined from the hawthorns that were naturally grown in the flora of Suşheri. At harvest time, the fruit, which would be adequate for pomological and biochemical measurements and analyzes, was harvested. According to the results of the measurements and analyzes in the study, fruit weight was found to vary between 0.68 g and 6.35 g, fruit width was between 10.52 and 29.48 mm and fruit length was between 11.40 and 20.67 mm. The highest firmness values were recorded with the genotype (G) 20 genotype, while the G4 had the lowest values in terms of the firmness values of the fruit flesh. While there are no significant differences between the pH values of the genotypes, the differences between the genotypes in terms of SSC, TA and vitamin C contents are quite significant. It has been found that there are significant differences between the genotypes in terms of total phenolic, total flavonoid and antioxidant activity. The total phenolic content ranged from 218.8 (G17) to 605.8 (G5 and G8) mg GAE kg–1 f.w., while the lowest total flavonoid content was 21.58 (G 17) and the highest total flavonoid content was 67.75 (G9) mg GAE kg–1 f.w. When the antioxidant activity was evaluated, the DPPH values were 1.08 (G17) – 15.43 (14) mmol TE kg–1 f.w., the FRAP values were 15.43 (G16) – 47.23 (G8) mmol TE kg–1 f.w. respectively.


LWT ◽  
2012 ◽  
Vol 46 (2) ◽  
pp. 548-555 ◽  
Author(s):  
Joanna Chlopicka ◽  
Pawel Pasko ◽  
Shela Gorinstein ◽  
Aneta Jedryas ◽  
Pawel Zagrodzki

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