scholarly journals In vitro antioxidant activity, phytochemical screening and total phenolic and flavonoid content from the leaves extracts of <em>Stachys germanica subs cordigera briq</em>

2020 ◽  
Author(s):  
Abdelmoughite Oaukil ◽  
Mohammed Lachkar ◽  
Soumaya El ismaili ◽  
Hanane El Hajaji ◽  
Amal Maurady ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Phenolic And Flavonoid ◽  
In Vitro Antioxidant Activity

scholarly journals Ekstrak Etanol Daun Belimbing Wuluh (Averrhoa bilimbi) sebagai Antioksidan dan Antiinflamasi

2019 ◽  
Vol 8 (3) ◽  
pp. 86
Author(s):  
Hasim Hasim ◽  
Yupi Yulianita Arifin ◽  
Dimas Andrianto ◽  
Didah Nur Faridah
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Phytochemical Compounds ◽  
Anti Inflammatory ◽  
Ethanol Extracts ◽  
Phenolic And Flavonoid

Belimbing wuluh merupakan tanaman jenis buah dan obat tradisional. Tanaman belimbing wuluh sudah sering dimanfaatkan masyarakat salah satunya untuk mengobati penyakit seperti batuk dan radang rektum. Tujuan penelitian ini adalah menguji senyawa fitokimia, menganalisis kandungan total fenolik dan flavonoid, serta aktivitas antioksidan dan antiinflamasi secara in vitro pada ekstrak etanol daun belimbing wuluh. Metode yang dilakukan pada penelitian ini adalah ekstraksi daun belimbing wuluh, skrining fitokimia, perhitungan total fenol dan flavonoid, uji aktivitas antioksidan, dan uji aktivitas antiinflamasi. Senyawa fitokimia yang terkandung pada ekstrak etanol daun belimbing wuluh adalah saponin, tanin, steroid, flavonoid, dan alkaloid. Kandungan total fenolik dan flavonoid ekstrak etanol daun belimbing wuluh secara berturut-turut sebesar 39,03 dan 97,28 µg QE/mg. Ekstrak etanol daun belimbing wuluh memiliki aktivitas antioksidan yang tergolong sangat kuat, sementara aktivitas antiinflamasinya terutama ditunjukkan pada konsentrasi ekstrak 200 µg/ ml, memiliki nilai persen inhibisi hemolisis yang paling tinggi. Kesimpulannya, ekstrak etanol daun belimbing wuluh dapat menjadi salah satu sumber antioksidan dan antiinflamasi alami.Ethanol Extracts of Averrhoa Bilimbi Leaf Demonstrated Antioxidative and Anti-inflammatory ActivityAbstractAverrhoa bilimbi, a fruit-bearing plant, has been traditionally used for medicinal purposes such as treatment of cough and rectal inflammation. In this current work, ethanol extract of Averrhoa bilimbi leaf was analyzed, with regard to phytochemical composition, i.e. total phenolic, and flavonoid, antioxidant activity, and in vitro antiinflammation activity. Extraction of carambola leaves, phytochemical screening, total phenolic and flavonoid contens, assay of antioxidant activity, and assay of anti-inflammatory activity were done in this research. As a result, the leaf extract positively contained some phytochemical compounds, i.e. saponin, tannin, steroid, flavonoid, and alkaloid. Furthermore, total phenolic and flavonoid of the leaf extract was found at 39.03 and 97.28 µg QE/mg extract, respectively. Additionally, antioxidant activity of the leaf extract was classified as very strong, while its anti-inflammatory feature at extract concentration of 200 µg/ml exhibited the highest inhibition of hemolysis. In summary, the ability of Averrhoa bilimbi leaf ethanol extracts to act as antioxidative and anti-inflammatory agents was determined and this may open the use for natural antioxidant and anti-inflammatory agents.

Extraction of Phenolic Compounds of six Algerian date (Phoenix dactylifera L.) cultivars from Ain-Saleh region, using Reflux method and Screening of Antioxidant Activity in vitro

2021 ◽  
pp. 161-167
Author(s):  
Habchi A. ◽  
Dekmouche M. ◽  
Hamia C. ◽  
Saidi M. ◽  
Yousfi M. ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Phenolic Compounds ◽  
Methanol Extract ◽  
Phoenix Dactylifera ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Phoenix Dactylifera L ◽  
Phenolic And Flavonoid

This study was designed to evaluate the antioxidant activity of phenolic compounds for six cultivars of date from In Saleh region (Phoenix dactylifera L.), which were extracted by reflux with solvents, 80% methanol and 70% aceton after the phenolic and flavonoid content were estimated. The total phenolic content (TPC) and total flavonoid content (TFC) of the extracts were measured using Folin–Ciocalteau and aluminum chloride colorimetric methods, respectively. Antioxidant activity acetone/H2O and methanol/H2O extracts using ABTS, DPPH, FRAP, TAC and CUPRAC assays. The results showed that methanolic solvent reflux gives better yields. The highest level of phenolic compounds for all dates varieties was found in the 80% methanol extract. This level ranged from 8.04 ± 0.14 to 46.81 ± 1.22 mg GAE/100 g fresh weight and flavonoid content was observed relatively low for both two solvents. However, extraction into 70% acetone gave the highest antioxidant activity with all assay. These results showed that dates had strongly scavenging activity on DPPH reaching 3.12 µg/mL in methanol/H2O from Cul1 (6.9 µg/mL with ascorbic acid), also on ABTS reaching 6.51 µg/mL in acetone/H2O from Cul2 (104.98 µg/mL with ascorbic acid).

scholarly journals Korelasi Antara Kadar Total Flavonoid dan Fenolik dari Ekstrak dan Fraksi Daun Jati Putih (Gmelina Arborea Roxb.) Terhadap Aktivitas Antioksidan

2019 ◽  
Vol 5 (1) ◽  
pp. 33-42
Author(s):  
Syamsu Nur ◽  
Fitriyanti Jumaetri Sami ◽  
Akbar Awaluddin ◽  
Mutiara Indah Ayu Afsari
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Colorimetric Method ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Gmelina Arborea ◽  
Flavonoid Content ◽  
Coefficient Corrélation ◽  
Phenolic And Flavonoid

This experiment aims to determine the correlation of total phenolic and flavonoid content of jati putih leaves fraction (Gmelina arborea Roxb.) towards Antioxidant activity .  Sample was extracted by maceration method using ethanol 70% to obtain the ethanol extract (EE), followed by liquid-liquid extraction method to obtain fraction of ethyl acetate (EA) and n-Hexane (EH). The phytochemical screening  and determination of total phenolic and flavonoid content were done by colorimetric method. Antioxidant activity were done by DPPH, FRAP and ABTS methods. Phytochemical screening showed positive results for flavonoids, phenolic and saponins.  The largest total phenolic content was found on EA (11,59 µg/ml ± 0,3 %b/b EAG) and the largest total flavonoid content was on EA (3,88 µg/ml ± 0,02 %b/b EK). The total phenolic and flavonoid content of Jati putih leaves has a correlation with antioxidant activity. The coefficient correlation of activity on reducingDPPH radical was 56,7% (total of phenolic content) and 57,8% (total of flavonoid content) and on iron reduction power in FRAP method  was 99,9% (total of phenolics and flavonoids content). The relationship with the activity in reducing radical ABTS obtained coefficient correlation of 57,0% and 58,1% for total phenolic and flavonoids contents, respectively.

scholarly journals A STUDY ON PHYTOCHEMICAL SCREENING, ANTIOXIDANT, ANTIMICROBIAL AND α-ΑMYLASE INHIBITORY ACTIVITIES OF CRUDE EXTRACTS OF THE STEM BARK OF PISONIA GRANDIS, R.BR

2017 ◽  
Vol 10 (11) ◽  
pp. 129
Author(s):  
Vishu Mohan ◽  
Mohan Kumar Ramasamy ◽  
Srikalyani Vemuri ◽  
Ilango Kaliappan
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
Antidiabetic Activity ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Phenolic And Flavonoid

  Objective: The aim of the present study is to determine the phytochemical screening, antioxidant activity and α-amylase inhibitory activity of the crude hexane, ethyl acetate and ethanolic stem bark extract of Pisonia grandis.Methods: The evaluation of antioxidant and antimicrobial activity, total phenolic, and flavonoid content were assessed using 2,2-diphenyl-1- picrylhydrazyl, Folin–Ciocalteu’s reagent, and aluminum chloride assay, respectively. The antidiabetic activity was assessed for porcine pancreatic α-amylase for the stem bark of P. grandis. Results: Phytochemical screening confirmed the presence of phenolic, flavonoids, tannins, saponins, terpenoids, and steroids in all the three extracts. The antioxidant activity showed 148.2 μg/ml, total phenolic content (gallic acid equivalent), 0.0665±0.0002 mg/g, flavonoid content (quercetin equivalent), 0.6061±0.1817 mg/g, and inhibitory concentration 50% values were found to be 40.42 μg/ml and showed better in ethyl acetate extract. The antidiabetic activity exhibited mimic action with insulin due to the presence of pinnatol in the stem bark and leaves of P. grandis. Conclusion: P. grandis stem bark crude ethyl acetate extract showed strong antioxidant activity, high phenolic, and flavonoid content. The antimicrobial activity was studied in both Gram-positive and Gram-negative strains against ampicillin and rifampicin as reference drugs. Antidiabetic activity shows effective result by α-amylase inhibitory activity.

scholarly journals PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT ACTIVITY OF WHOLE PLANT EXTRACTS OF SESUVIUM PORTULACASTRUM L

2018 ◽  
Vol 11 (1) ◽  
pp. 322
Author(s):  
Sathvika Chintalapani ◽  
Swathi M. S. ◽  
Mangamoori Lakshmi Narasu
Keyword(s):  
Antioxidant Activity ◽  
Diethyl Ether ◽  
Dry Weight ◽  
Total Phenolic ◽  
Ether Extract ◽  
Sesuvium Portulacastrum ◽  
Flavonoid Content ◽  
Diethyl Ether Extract ◽  
Phenolic And Flavonoid

  Objectives: The objectives of this study are to screen the phytochemicals, estimate the content of phenolic and flavonoid compounds, and determine the antioxidant capacity of the Sesuvium portulacastrum L. (S. portulacastrum).Methods: The crude bioactives were extracted from the dried powder of S. portulacastrum in an orbital shaker using ethanol, methanol, acetone, hexane, and diethyl ether solvents. Rotaevaporator was used to concentrate the extracts. Total phenolic and flavonoid content was estimated spectrophotometrically using Folin-Ciocalteu and aluminum chloride reagents, respectively. 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), hydrogen peroxide (H2O2), and ferric reducing antioxidant power (FRAP) assays were used to determine the in vitro antioxidant capacity.Results: Phytochemical screening of the extracts showed the presence of major classes of phytochemicals. Total phenolic content (TPC) expressed as gallic acid equivalents (GAE) ranged from 14.42 (ethanol extract) to 54.05 (diethyl ether extract) mg GAE/g dry weight. Total flavonoid content expressed as quercetin equivalents (QE) ranged from 22.03 (hexane extract) to 56.70 (methanol extract) mg QE/g dry weight. Antioxidant activity determined by different assays is highest in diethyl ether extract. A positive correlation (0.7241≤ r ≥0.8419) was found between the TPC and antioxidant activity determined by DPPH, ABTS, and H2O2 scavenging assays. Furthermore, there is a strong correlation (0.722≤ r ≥0.999) between all the pairs of antioxidant assays.Conclusion: Diethyl ether extract showed the highest TPC and antioxidant potential among all the extracts of S. portulacastrum. Further research has to be done to isolate the pure bioactive compound that has high antioxidant potential.

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