scholarly journals Pengaruh Konsentrasi Asam Cuka terhadap Sporulasi Beauveria bassiana (Bals.) Vuill Strain-Wamena pada Medium Beras Pera Sebagai Agen Hayati

2018 ◽  
Vol 1 (2) ◽  
pp. 81-88
Author(s):  
Rini Patandungan ◽  
Rosye H.R. Tanjung ◽  
Mesak Kamarea

This study carried out at Laboratory of Biology, Division of Food and Cultivated Plants Protection, Jayapura from January-June 2008. The objective of this study was to examine the effect of citric acid (CH3COOH) concentration on rate of growth and spore production of Beauveria bassiana (Bals.) Vuill. strain Wamena which grow in short grain rice (beras pera) media. Six different concentration of citric acid (CH3COOH)  were 0; 0,025%; 0, 05%; 0,075%; 0,1% and 0,125% with 5 replicates for each concentration, complete random design were used. Result of this study showed that 0.05% of citric acid significantly affected spore production which mean potential  for biological  control  agent. Key words: Beauveria bassiana (Bals.) Vuill Strain Wamena, rice medium, biological control agent. 

Author(s):  
Ines Borgi ◽  
Jean-William Dupuy ◽  
Imen Blibech ◽  
Delphine Lapaillerie ◽  
Anne-Marie Lomenech ◽  
...  

1977 ◽  
Vol 67 (4) ◽  
pp. 627-633 ◽  
Author(s):  
A. J. Wapshere ◽  
A. A. Kirk

AbstractThe gracillariid leaf miner Dialectica scalariella (Zell.), which occurs in Mediterranean Europe, North Africa, the Middle East and the Canaries, is a potential biological control agent for the weed Echium plantagineum in Australia. The moth has a high fecundity and oviposits on the leaves of Echium spp. and other Boraginaceae. The larvae destroy the leaves by forming large bulbous blotch mines. There are 5-7 generations per year. Host restriction of D. scalariella to Boraginaceae was tested by exposing a wide range of cultivated plants considered to be most at risk from it because of their close relation to Boraginaceae, because they were of Australian or non-European origin, because they did not occur in the same ecoclimatic region as D. scalariella, because their entomological fauna was poorly known, and because they or related plants were known to be attacked by insects closely related to D. scalariella. The host restriction of the moth was confirmed as only boraginaceous plants were attacked. It was concluded that both in terms of effectiveness and specificity D. scalariella could serve as a biological control agent in Australia for E. plantagineum.


2020 ◽  
Vol 8 (6) ◽  
pp. 730-742
Author(s):  
Manish Dhawan ◽  
◽  
Neelam Joshi ◽  
Samandeep Kaur ◽  
Saroop Sandhu ◽  
...  

Intensive crop production and extensive use of harmful synthetic chemical pesticides create numerous socio-economic problems worldwide. Therefore, sustainable solutions are needed for insect pest control, such as biological control agents. The fungal insect pathogen Beauveria bassiana has shown considerable potential as a biological control agent against a broad range of insects. The insight into the virulence mechanism of B. bassiana is essential to show the robustness of its use. B. bassiana has several determinants of virulence, including the production of cuticle-degrading enzymes (CDEs), such as proteases, chitinases, and lipases. CDEs are essential in the infection process as they hydrolyze the significant components of the insect's cuticle. Moreover, B. bassiana has evolved effective antioxidant mechanisms that include enzyme families that act as reactive oxygen species (ROS) scavengers, e.g., superoxide dismutases, catalases, peroxidases, and thioredoxins. In B. bassiana, the number of CDEs and antioxidant enzymes are characterized in recent years. These enzymes are believed to be crucial player of evolutionary process in this organism and their role in various mechanism of biological control. Recent discoveries have significantly increased our potential understanding on several potentially wanted unknown mechanisms of B. bassiana infection. This review focuses on the progress detailed in the studies of these enzymes and provides an overview of enzymatic activities and their contributions to virulence.


2021 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Rina Novianti ◽  
Hafiz Fauzana ◽  
Rusli Rustam

The Oryctes rhinoceros pest is an important pest of palm oil plant.  Pest problems occur because  Oil Palm Empty Fruit Bunches (OPEFB) is added for soil fertility to create a breeding site for larvae O. rhinoceros. Generally, OPEFB will receive the plants more quickly when composted, and pest control is carried out in the compost. Biological control is more recommended because it is environmentally friendly, Therefore compost is added with biological control agent O. rhinoceros namely B. bassiana. This study is aimed to obtain the best conidia density of Beauveria bassiana in compost in controlling larvae O. rhinoceros. The research was carried out at the Plant Pest Laboratory and Experimental Garden, Faculty of Agriculture, Riau of University. The study was carried out from February to November 2020. The experiment on the conidia density of B. bassiana fungi in compost media against larvae O. rhinoceros, using a Completely Randomized Design (CRD), with 6 treatments 4 replications obtained 24 experimental units, while the treatments were 0 g.l-1, 15 g.l-1, 30 g.l-1, 45 g.l-1, 60 g.l-1 and 75 g.l-1. The results of the research revealed that  OPEFB compost + sawdust containing the fungus B. bassiana 75 g.l-1 (83,2 x108 kon/ml) had the best ability to control larvae O. rhinoceros with a total larvae mortality of 87% which caused early death of 54 hours after application, LT50 of 213 hours after application, and LC50 of 3,3% or the equivalent of 33 g.l-1 at 14 days after application.


2020 ◽  
Vol 11 (3) ◽  
pp. 94
Author(s):  
DWI ADI SUNARTO ◽  
NURINDAH NURINDAH ◽  
SUJAK SUJAK

<p>ABSTRAK<br />Pectinophora gossypiella merupakan salah satu hama utama<br />tanaman kapas yang menyerang dengan cara menggerek buah. Mulai<br />stadia larva kecil hingga pupa berada di dalam buah. Perilaku tersebut<br />menjadi salah satu sebab kurang efektifnya beberapa cara pengendalian P.<br />gossypiella dengan sasaran stadia larva. Untuk itu, peluang yang<br />diharapkan akan memberikan hasil pengendalian yang lebih baik adalah<br />sasaran pada stadia telur yaitu antara lain pemanfaatan parasitoid telur.<br />Dari hasil eksplorasi telah diperoleh parasitoid telur Trichogrammatidae<br />yang berasal dari pertanaman kapas di Lamongan dan Asembagus, Jawa<br />Timur. Penelitian ini bertujuan untuk mengidentifikasi spesies parasitoid<br />telur P. gossypiella dan kapasitas reproduksinya. Penelitian ini<br />dilaksanakan di Balai Penelitian Tanaman Tembakau dan Serat Malang<br />pada bulan Maret - Desember 2002. Hasil penelitian menunjukkan bahwa<br />parasitoid telur Trichogrammatidae yang muncul dari telur P. gossypiella<br />yang berasal dari kedua lokasi, berasal dari spesies yang sama dan berbeda<br />dengan spesies T. armigera yang memarasit telur H. armigera.<br />Berdasarkan kapasitas reproduksinya, Trichogrammatoidea bactrae N &amp; N<br />berpotensi sebagai agens hayati pengendali ulat penggerek buah kapas<br />merah jambu P. gossypiella.<br />Kata kunci : Kapas,  Gossypium  hirsutum,  hama,  Pectinophora<br />gossypiella,  parasitoid  telur,  Trichogrammatidae,  laju<br />pertumbuhan</p><p><br />ABSTRACT<br />Identification and reproduction capacity test of egg<br />parasitoid pink bollworm, Pectinophora gossypiella<br />Saunders on cotton<br />Pectinophora gossypiella is one of main pests of cotton. It attacks<br />the boll since small larvae until pupae and the insect is located in the boll.<br />This could be the reason why any control actions targeted to the larvae<br />were not effective. Therefore, the use of egg parasitoid to control the<br />bollworm population would be more promising. Exploration of the egg<br />parasitoid of the bollworm was done in Lamongan and Asembagus, East<br />Java. The parasitoids were considered as new locality report. This study<br />was to identify egg parasitoid of P. gossypiella and to study their<br />reproduction capacity. The study was conducted in Biological Control<br />Laboratory of IToFCRI Malang, March – December 2002. The study<br />included identification morphologically and biosystematically. The results<br />showed that Trichogrammatid emerged from P. gossypiella egg collected<br />from Asembagus (Trichogrammatoidea sp-A) and Lamongan (Trichogra-<br />mmatoidea sp-L) were the same species. Based on the reproduction<br />capacity of the parasitoids, Trichogrammatoidea bactrae N &amp; N were<br />potential as biological control agent for P. gossypiella.<br />Key words : Cotton,  Gossypium  hirsutum,  pest,  Pectinophora<br />gossypiella, egg parasitoid, Trichogrammatidae, intrinsic<br />rate</p>


2005 ◽  
Vol 40 (1) ◽  
pp. 67-73 ◽  
Author(s):  
L. A. Rodríguez-del-Bosque ◽  
F. Silvestre ◽  
V. M. Hernández ◽  
H. Quiroz ◽  
J. E. Throne

Five isolates of Beauveria bassiana (Balsamo) Vuillemin and 3 isolates of Metarhizium anisopliae (Metschnikoff) Sorokin were tested against third-instar larvae of Phyllophaga crinita (Burmeister) and Anomala flavipennis Burmeister under laboratory conditions using the “maximum challenge test” protocol. The M. anisopliae strains were more virulent than the B. bassiana strains, with the isolates MAGL3N and MAGL4N of M. anisopliae causing the highest mortality in both white grub species. Regardless of scarab species, mortality caused by MAGL3N was &gt;63% after 4 days and &gt;96% after 10 days. The other strain of M. anisopliae, MAGC2N, also caused high mortality in A. flavipennis, but at a slower rate than MAGL3N. Median lethal time (LT50) for MAGL3N was 2.9 days for P. crinita and 3.0 d for A. flavipennis. The LT50 for MAGL4N was 5.3 d for P. crinita and 7.6 d for A. flavipennis while the LT50 for MAGC2N was 4.4 d for A. flavipennis. Metarhizium anisopliae is a potential biological control agent for P. crinita and A. flavipennis and should be further investigated for possible development.


2009 ◽  
Vol 50 (3) ◽  
pp. 329-335 ◽  
Author(s):  
Sthenia Santos Albano Amóra ◽  
Claudia Maria Leal Bevilaqua ◽  
Francisco Marlon Carneiro Feijó ◽  
Mariana Araújo Silva ◽  
Romeika Hermínia Macedo Assunção Pereira ◽  
...  

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