NMRI Mouse | ScienceGate

(±)-Aeroplysinin-1, an optically active 1.2-dihydroarene-1.2-diol. was isolated from the marine sponges Verongia aerophoba (+-isomer) and lanthella ardis (--isomer). For the experiments presented we used the +-isomer from Verongia aerophoba. Here we describe the hitherto unknown biological and pharmacological property of this compound to display pronounced anticancer activity against L5178y mouse lymphoma cells (ED50: 0.5 μm). Friend erythroleukemia cells (ED50: 0.7μm) , human mamma carcinoma cells (ED50: 0.3μm) and human colon carcinoma cells (ED50: 3.0 μm) in vitro. Furthermore, aeroplysinin caused a preferential inhibition of [3H]thymidine (dThd) incorporation rates in L5178y mouse lymphoma cells if compared with murine spleen lymphocytes in vitro. At concentrations between 1.1 and 28.5 μm, the [3H]dThd incorporation rates in L5178y cells were suppressed to 28% -0% but only to 78% -18% in murine spleen lymphocytes. The same differential effect in vitro was found with the following epithelial cells: 14.70 μm of the compound were required to inhibit normal human fibroblasts to 50% , but only 2.9 μm in the assays with human malign keratinocytes or malignant melanoma cells to observe the same inhibitory effect. Moreover, aeroplysinin-1 displayed antileukemic activity in vivo using the L5178y cell/NMRI mouse system; administered at a dose of 50 mg/kg for five consecutive days, the T/C (% ) value was determined to be 338. Preliminary toxicology studies revealed an acute LD50 of 202 mg/kg and a subacute LD50 of 150 mg/kg. Aeroplysinin-1 is neither a direct mutagen nor a premutagen in the umu/Salmonella typhimurium test system.

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Experimental Aspects of the Pathogenesis of Robin Sequence

The Cleft Palate-Craniofacial Journal ◽

10.1597/03-166.1 ◽

2005 ◽

Vol 42 (4) ◽

pp. 372-376 ◽

Cited By ~ 14

Author(s):

Johannes Schubert ◽

Heiko Jahn ◽

Markus Berginski

Keyword(s):

Nmri Mouse ◽

Primary Role ◽

Pierre Robin Sequence ◽

Mandibular Growth ◽

Robin Sequence ◽

Secondary Palate ◽

Tongue Position ◽

Mouse Fetuses ◽

Mandibular Retrognathia ◽

Pierre Robin

Objective The Pierre Robin Sequence (PRS) is a good example of disturbed embryonic development of the secondary palate involving insufficient mandibular growth, failed forward tongue movement, and, in the case of a cleft, impeded fusion of the secondary palate. Discussion continues regarding which of the involved pathogenetic factors is the primary cause of the induced cascade of signs: insufficient mandibular growth or failed descent of the tongue. Design Forty-five randomly selected, 18-day-old formalin-fixed A/WySn mouse fetuses were investigated. The strain is known to have a basic genetic defect and as much as 44% clefts in the offspring. Twenty-four fetuses in the group had a cleft palate. Mandible position was measured relative to the head and to the presence or absence of a cleft. Cleft width and tongue position were also determined. Thirty-eight NMRI mouse fetuses of the same age served as controls. Results All A/WySn fetuses showed marked mandibular retrognathia, which was more severe in the cleft group (p < .05), but there was no correlation between the degree of retrognathia and cleft width. The median cleft width was 3.4 mm (1.6 through 6.3 mm). The tongue was in the cleft in all 12 fetuses with wide clefts (>3.4 mm wide), and free in the oral cavity in the other 12. Tongue position did not influence the degree of retrognathia (p < .05). Moreover, the tongue was free in all fetuses with severe retrognathia. Conclusion The results support the primary role of retroposition of the mandible in the development of cardinal symptoms of Pierre Robin Sequence.

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Stereoselective distribution of the teratogenic thalidomide analogue EM12 in the early embryo of marmoset monkey, Wistar rat and NMRI mouse

Archives of Toxicology ◽

10.1007/s002040050336 ◽

1996 ◽

Vol 70 (11) ◽

pp. 749-756 ◽

Cited By ~ 15

Author(s):

H.-J. Schmahl ◽

Lennart Dencker ◽

Claudia Plum ◽

Ibrahim Chahoud ◽

Heinz Nau

Keyword(s):

Wistar Rat ◽

Early Embryo ◽

Nmri Mouse ◽

Marmoset Monkey ◽

Thalidomide Analogue

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Antioxidant and Anti-Inflammatory Activities of Caralluma Acutangula (Decne.) N.E.Br Extracts, a Medicinal Plant from Burkina Faso

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v9i6.3720 ◽

2019 ◽

Vol 9 (6) ◽

pp. 155-161

Author(s):

Pare Dramane ◽

N’do Jotham Yhi-pênê ◽

Hilou Adama

Keyword(s):

Oxidative Stress ◽

Hydrogen Peroxide ◽

Lipid Peroxidation ◽

Secondary Metabolites ◽

Acute Toxicity ◽

Xanthine Oxidase ◽

Antioxidant Systems ◽

Nmri Mouse ◽

Good Activity ◽

Anti Inflammatory

Plants have always played an important role in health care in Africa. The stress, a situation of imbalance between oxidizing and antioxidant systems in favor of the prooxidants is responsible for the installation of several pathologies such as cancers, cardiovascular diseases, diabetes ... The objective of this study was to highlight the presence Secondary metabolites in C. acutangula extract and determine its antioxidant and anti-inflammatory potential. For the determination of the acute toxicity of the extract, a dose of 2000 mg / kg was administered to the NMRI Mouse. The methods of screening were used to detect secondary metabolites like tannins, steroids and terpen, flavonoids, coumarins. The antioxidant capacity was evaluated in vitro by determining the ability of the extract to inhibit lipid peroxidation, hydrogen peroxide, degradation of deoxyribose. The anti-inflammatory potential was evaluated on lipoxygenase and xanthine oxidase. Acute toxicity evaluated in NMRI mice showed that the ethanolic extract of C. acutangula show no toxicity. Tannins, steroids and terpen, flavonoids, coumarins have been detected in the extracts. C. acutangula showed good activity with an inhibition of 50.71 ± 2.51% at 100 μg / ml on lipid peroxidation, of 66.105 ± 1.26% on deoxyribose degradation and 8.625 ± 1.09% on hydrogen peroxide. It showed good activity on xanthine oxidase with an 81.5 ± 5.5% inhibition. For the effect on lipoxygenase it gave an inhibition of the enzyme at 43.11 ± 3.4%. This potential could be used in the fight against inflammatory diseases and that due to oxidative stress. Keywords: antioxidant, anti-inflammatory, oxidative stress, lipid peroxidation

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Fresh and cultured mouse islets differ in their response to nutrient stimulation

Endocrine Connections ◽

10.1530/ec-20-0289 ◽

2020 ◽

Vol 9 (8) ◽

pp. 769-782

Author(s):

Mai Morsi ◽

Torben Schulze ◽

Eike Früh ◽

Dennis Brüning ◽

Uwe Panten ◽

...

Keyword(s):

Insulin Secretion ◽

Culture Conditions ◽

Nmri Mouse ◽

Fluorescence Ratio ◽

Mouse Islets ◽

Stimulus Secretion Coupling ◽

Apoptosis And Necrosis ◽

Endogenous Metabolites ◽

Kinetics Of ◽

Stimulation Of

Observing different kinetics of nutrient-induced insulin secretion in fresh and cultured islets under the same condition we compared parameters of stimulus secretion coupling in freshly isolated and 22-h-cultured NMRI mouse islets. Stimulation of fresh islets with 30 mM glucose after perifusion without nutrient gave a continuously ascending secretion rate. In 22-h-cultured islets the same protocol produced a brisk first phase followed by a moderately elevated plateau, a pattern regarded to be typical for mouse islets. This was also the response of cultured islets to the nutrient secretagogue alpha-ketoisocaproic acid, whereas the secretion of fresh islets increased similarly fast but remained strongly elevated. The responses of fresh and cultured islets to purely depolarizing stimuli (tolbutamide or KCl), however, were closely similar. Signs of apoptosis and necrosis were rare in both preparations. In cultured islets, the glucose-induced rise of the cytosolic Ca2+ concentration started from a lower value and was larger as was the increase of the ATP/ADP ratio. The prestimulatory level of mitochondrial reducing equivalents, expressed as the NAD(P)H/FAD fluorescence ratio, was lower in cultured islets, but increased more strongly than in fresh islets. When culture conditions were modified by replacing RPMI with Krebs–Ringer medium and FCS with BSA, the amount of released insulin varied widely, but the kinetics always showed a predominant first phase. In conclusion, the secretion kinetics of fresh mouse islets is more responsive to variations of nutrient stimulation than cultured islets. The more uniform kinetics of the latter may be caused by a different use of endogenous metabolites.

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