Histological Procedure

Although the occurrence of muscle spindles (MS) is quite high in most skeletal muscles of humans, few MS, or even absence, have been reported in digastric and mylohyoideus muscles. Even if this condition is generally accepted and quoted in many papers and books, observational studies are scarce and based on histological sections of a low number of specimens. The aim of the present study is to confirm previous data, assessing MS number in a sample of digastric and mylohyoideus muscles. We investigated 11 digastric and 6 mylohyoideus muscles from 13 donors. Muscle samples were embedded in paraffin wax, cross-sectioned in a rostrocaudal direction, and stained using haematoxylin-eosin. A mean of 5.1 ± 1.1 (range 3–7) MS was found in digastric muscles and mean of 0.5 ± 0.8 (range 0–2) in mylohyoideus muscles. A significant difference (P<0.001) was found with the control sample, confirming the correctness of the histological procedure. Our results support general belief that the absolute number of spindles is sparse in digastric and mylohyoideus muscles. External forces, such as food resistance during chewing or gravity, do not counteract jaw-opening muscles. It is conceivable that this condition gives them a limited proprioceptive importance and a reduced need for having specific receptors as MS.

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A histological procedure to study fungal infection in the wax moth Galleria mellonella

European Journal of Histochemistry ◽

10.4081/ejh.2014.2428 ◽

2014 ◽

Vol 58 (3) ◽

Cited By ~ 31

Author(s):

F. Perdoni ◽

M. Falleni ◽

D. Tosi ◽

D, Cirasola ◽

S. Romagnoli ◽

...

Keyword(s):

Fungal Infection ◽

Galleria Mellonella ◽

Histological Procedure ◽

Wax Moth

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Adaptation of the Relationship in the "Parasite–Host" System with Parasitism Paramphistomum сervi in the Small Intestine of Sheeps

Russian Journal of Parasitology ◽

10.31016/1998-8435-2020-14-1-46-52 ◽

2020 ◽

Vol 14 (1) ◽

pp. 46-52

Author(s):

Oksana I. Bibik ◽

Ivan A. Arkhipov ◽

Lyubov V. Nacheva ◽

Miсhail S. Boborykin

Keyword(s):

Small Intestine ◽

Mucous Membrane ◽

Microstructural Analysis ◽

Epithelial Tissue ◽

Host System ◽

Mutual Relations ◽

The Relationship ◽

Histological Procedure ◽

Histological Staining

The purpose of the research is studying microstructural changes in the process of adaptation of components during the formation of the “parasite–host” system by the example of Paramphistomum cervi (Zeder, 1790) parasitizing in the small intestine of spontaneously infected sheep. Materials and methods. Peculiarities of changes in the microstructure of the small intestine in the “parasite–host” system were studied using parasitization of P. cervi in the intestines of sheep using well-known histological methods. Pieces of tissue of the small intestine of sheep with P. cervi, after being preserved in 70% alcohol, were processed according to the generally accepted histological procedure and embedded in paraffin. Sections 5–7 μm in thickness were stained with histological stains and examined under a light microscope. Results and discussion. Microstructural analysis of the characteristics of the relationship in the "parasite–host" system showed that the mucous membrane of the small intestine of sheep in the presence of P. cervi looks sharply thickened. Swelling of the epithelium of villi and crypts, its vacuolization and albuminoid degeneration were found. In some places, proliferation of the epithelial layer of the mucous membrane was detected, due to which epithelial hyperplasia is observed, in some cases turning into metaplasia. Proliferation and hyperplasia (metaplasia) in the endostation of the host in the presence of the parasite contribute to: 1. stability of the parasite-host system and the participation of trematodes in trophism; 2. determination of the clinical and morphological picture of trematodose; 3. predicting the effects of this pathology on the host. Adhesion in the microstructural complex “trematode tegument – epithelial tissue of the villi of the small intestine” in ovine paramphistomosis at the contact level of two glycocalyx layers – the tegument of P. cervi and the surface of the epithelial cells of the villi of the host’s intestine, as well as single histological staining of the sites of contact between the parasite and the host as a result of mixing the components of the trematode tegument and the tissue of the host’s small intestine with deep adhesion indicate the established mutual relations between the components of a single system "parasite–host".

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Aggregation of hepatic melanomacrophage centers in S. herzbergii (Pisces, Ariidae) as indicators of environmental change and well-being

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/1678-4162-12327 ◽

2021 ◽

Vol 73 (4) ◽

pp. 868-876

Author(s):

H.C. Viana ◽

W.B. Jesus ◽

S.K.L. Silva ◽

M.B. Jorge ◽

D.M.S. Santos ◽

...

Keyword(s):

Young People ◽

Environmental Conditions ◽

Rainy Season ◽

Well Being ◽

Biomarker Of Exposure ◽

Xenobiotic Biotransformation ◽

Sciades Herzbergii ◽

Histological Procedure ◽

Hematoxylin Eosin ◽

Different Levels

ABSTRACT The melanomacrophage centers (MMCs) in the liver of fish are indicators of environmental conditions, as they are involved in xenobiotic biotransformation. The objective of this study was to evaluate the number of MMC in the liver of juveniles and adults of Sciades herzbergii from areas with different levels of contamination. The fish were caught at three points (reference - A1, potentially impacted - A2 and contaminated - A3), in São José bay (Maranhão, Brazil), in four samples. The livers were subjected to the standard histological procedure and 5μm sections were stained with hematoxylin-eosin. In livers of A2 adult individuals (260.50±161.50 MMCs / mm²) they presented a greater number of MMCs when compared to A3 adults (60.00 ± 30.10 MMCs / mm²). Juveniles showed considerable values in A1 (100.00 ± 0.00 MMCs/mm²) and A2 (95.33 ± 33.00 MMCs / mm²) compared to juveniles in A3 (49.00±0.00 MMCs/mm²). These high values are unexpected for young people. The average number of MMC correlated with the rainy season in the region. The use of hepatic MMCs as a biomarker of exposure to pollutants, in particular substances from fisheries systems, such as ammonia and nitrite, proved to be adequate to differentiate areas with different levels of impacts.

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Evaluation of different haematoxylin stain subtypes for the optimal microscopic interpretation of cutaneous malignancy in Mohs frozen section histological procedure

British Journal of Biomedical Science ◽

10.1080/09674845.2020.1838075 ◽

2020 ◽

pp. 1-9

Author(s):

JA Gabriel ◽

M Shams ◽

GE Orchard

Keyword(s):

Frozen Section ◽

Cutaneous Malignancy ◽

Microscopic Interpretation ◽

Histological Procedure

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Adaptation of certain Histological Techniques for in situ Demonstration of the Neuro-endocrine system of insects and other animals

Journal of Cell Science ◽

10.1242/jcs.s3-105.72.455 ◽

1964 ◽

Vol s3-105 (72) ◽

pp. 455-466

Author(s):

G. S. DOGRA ◽

B. K. TANDAN

Keyword(s):

Endocrine System ◽

Staining Technique ◽

Blue Staining ◽

Neurosecretory Material ◽

Whole Brain ◽

Histological Techniques ◽

Aldehyde Fuchsin ◽

Staining Techniques ◽

Histological Procedure

Three techniques for staining the secretory neurones in sections were applied directly to the whole brain and/or intact organs of the neuro-endocrine system of certain insects, and the whole brain of various invertebrates and vertebrates. After minor changes in the original procedures, in situ staining was achieved in those components of the neuro-endocrine system that are known to contain the neuro-secretory material. With the Victoria blue staining technique, the secretory neurones, the neurosecretory pathway, and the storage-and-release organ were stained satisfactorily in all the experimental animals, in such a way that observations could be made in whole mounts or suitably dissected portions of the bulk-stained preparations. With the aldehyde-fuchsin and aldehyde-thionin staining techniques, the somata and the proximal portion of the axon of the neurones and the storage-and-release organ were usually stained satisfactorily enough for purposes of observation in the invertebrate material only. On sectioning the bulk-stained components of the neuroendocrine system and mounting the sections, the sites known to contain the neurosecretory material were revealed promptly. On comparing the information derived from mounts of the bulk-stained preparations with that derived from sections of similar preparations, and also with that derived from routine histological procedure, no difference was detected.

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