Application of a multispectral assay for evaluation of the morphofunctional status of immunocompetent cells in cattle with retroviral diseases

Abstract. Microspectral analysis is a highly informative way to study the morphofunctional status of cells, including immunocompetent ones. Purpose of the study was an application it for studding of bovine lymphocytes in cattle with enzootic leukemia and viral immunodeficiency, which is important for diagnostics and differential diagnostics of diseases, as well as for analyzing of animal homeostasis and making a prognosis of the disease. Leukemia and immunodeficiency are classified as hematopathological processes. Therefore, we used the hematological method as a comparative method. Spectral analysis of stained with a Leukodif 200 kit agranulocytes was carried out using the universal color analyzer microscope-spectrophotometer LOMO MSFU-K. The value of the light absorption intensity was measured in the visible-light spectrum from 300 to 700 nm. The results of microspectral analysis showed that for cows’ lymphocytes with combined pathology (BLV/BIV), the absorption values in the spectrum of eosin U and azure II were 351.2 ± 17.6 and 751.4 ± 37.6 counts, respectively. For animals with BLV and BIV mono-infection, these indicators were 253.3 ± 12.7; 383.3 ± 19.2 and 371.5 ± 18.5; 500.2 ± 24.9 counts. While in intact cows, these indicators were registered at the level of 210.3 ± 10.5 and 173.6 ± 8.6 counts. In intact animals, the acidic and basic components ratio in the cell was uniformly proportional, the ratio coefficient was 0.83 ± 0.04. For BIV-, BLV- and BLV/BIV-infected animals, this coefficient was 1.34 ± 0.06, 1.51 ± 0.08 and 2.13 ± 0.11, that was in 1.6, 1.8 and 2.6 times higher than in intact animals, which might be an indicator of metabolic acidosis in the cell. The scientific novelty of the research is that the signs of hypoxia and inflammation detected by hematological analysis correlate with the results of microspectral analysis and indicate a change in the homeostasis of the infected animal’s organism, including at the cell level.

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Analysis of Business Cycles in the Breeding of Pigs, Cattle and Poultry and their Relationship to the Causality of Wheat and Rye Cultivation in Poland

Zeszyty Naukowe SGGW w Warszawie - Problemy Rolnictwa Światowego ◽

10.22630/prs.2018.18.2.49 ◽

2018 ◽

Vol 18(33) (2) ◽

pp. 218-227

Author(s):

Mateusz Mierzejewski ◽

Magdalena Lampart

Keyword(s):

Time Series ◽

Spectral Analysis ◽

Business Cycles ◽

Comparative Method ◽

Phase Spectrum ◽

Cyclical Relationships ◽

Polish Economy ◽

And Shifts ◽

The Relationship ◽

Poultry Farming

The article presents a study on the phenomenon of pig gaps in the perspective of pig, cattle, and poultry farming in Poland. The work attempts to define the phenomenon itself as well as to show the reasons for its occurrence and significance for the Polish economy. The study used a cross-spectral analysis, which indicated cyclical relationships and shifts between the studied time series. The methodology of the work was based on a simplified spectral analysis, i.e. the use of the square of coherence, spectral density and phase spectrum. In addition, the article uses a comparative method for selected production volumes. The results were analyzed in the context of occurrence of pig cycles for breeding and cultivation. The study showed the occurrence of the relationship between pig breeding and wheat and rye cultivation.

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Systematic zone-Axis orientation of NM-scale particles for microdiffraction

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100174448 ◽

1990 ◽

Vol 48 (4) ◽

pp. 262-263

Author(s):

E D Boyes ◽

L Hanna

Keyword(s):

Real Space ◽

Dark Field ◽

Zone Axis ◽

High Symmetry ◽

Lattice Imaging ◽

Cell Level ◽

Bright Field ◽

Axis Orientation ◽

Annular Dark Field ◽

Target Designation

A VG HB501 FEG STEM has been modified to provide track whilst tilt [TWIT] facilities for controllably tilting selected and initially randomly aligned nanometer-sized particles into the high symmetry zone-axis orientations required for microdiffraction, lattice imaging and chemical microanalysis at the unit cell level. New electronics display in alternate TV fields and effectively in parallel on split [+VTR] or adjacent externally synchronized screens, the micro-diffraction pattern from a selected area down to <1nm2 in size, together with the bright field and high angle annular dark field [HADF] STEM images of a much wider [˜1μm] area centered on the same spot. The new system makes it possible to tilt each selected and initially randomly aligned small particle into a zone axis orientation for microdiffraction, or away from it to minimize orientation effects in chemical microanalysis. Tracking of the inevitable specimen movement with tilt is controlled by the operator, with realtime [60Hz] update of the target designation in real space and the diffraction data in reciprocal space. The spot mode micro-DP and images of the surrounding area are displayed continuously. The regular motorized goniometer stage for the HB501STEM is a top entry design but the new control facilities are almost equivalent to having a stage which is eucentric with nanometric precision about both tilt axes.

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