scholarly journals Molecular Detection of HSV type 2 Infection among Infertile Males in Khartoum State, Sudan

2021 ◽  
Vol 10 (5) ◽  
Author(s):  
Mai Elhadi ◽  
Abdel Rahim M El Hussein ◽  
Mohamed O Mustafa ◽  
Isam M Elkhidir ◽  
Khalid A Enan
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Sperm Count ◽  
Ancient Greece ◽  
Rt Pcr ◽  
Dna Viruses ◽  
Double Stranded Dna ◽  
Polymerase Chain ◽  
Simplex Virus

Background:  Herpes simplex virus (HSV) infections of humans were first documented in ancient Greece.  HSVs belong to family Herpesviridae, and are characterized by being enveloped, double-stranded DNA viruses with relatively large complex genomes.  Herpes simplex virus type 2 (HSV-2) DNA seems to play a significant role in male infertility, it is significantly related to low sperm count as well as poor motility. Aim: This study aimed to determine the incidence of HSV-2 DNA among infertile males in Khartoum State, Sudan by using molecular methods. Methods: The study was carried out in Khartoum State, Sudan, during May-August 2018. A total of 50 semen samples from infertile males were included. HSV-2 was detected using real-time polymerase chain reaction (RT-PCR). Result: Among 50 semen samples from infertile males, 6(12%) samples were found positive for HSV-2 DNA. Conclusion: Our findings showed no association between HSV-2 DNA and low sperm count in infertile Sudanese men. Our study also revealed the need for further investigations in different parts of the country to highlight the extent of the viral related men infertility problem in Sudan. Keywords: HSV-2, RT-PCR, infertility, men, Sudan

scholarly journals PREVALENCE OF HERPES SIMPLEX VIRUS TYPE 2 AND RISK FACTORS ASSOCIATED WITH THIS INFECTION IN WOMEN IN SOUTHERN BRAZIL

2013 ◽  
Vol 55 (5) ◽  
pp. 315-321 ◽  
Author(s):  
Thais Duquia Moraes Caldeira ◽  
Carla Vitola Goncalves ◽  
Gisele Rodrigues de Oliveira ◽  
Tania Vieira da Fonseca ◽  
Regina Goncalves ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Pap Smear ◽  
Rio Grande ◽  
Immunodeficiency Virus ◽  
Polymerase Chain ◽  
Simplex Virus

SUMMARY The herpes simplex virus type 2 (HVS-2) is the most prevalent infection worldwide. It is a cofactor in the acquisition of human immunodeficiency virus (HIV) and the persistence of human papillomavirus (HPV). This study evaluated the prevalence of HSV-2, using the polymerase chain reaction (PCR), and associated factors in patients treated at the Federal University of Rio Grande (FURG) and Basic Health Units (BHU) in Rio Grande, Brazil. The observed prevalence of HSV-2 was 15.6%. Among the 302 women studied, 158 had received assistance in BHU and 144 were treated at FURG. The prevalence of HSV-2 in these groups was 10.8% and 20.8%, respectively, RR 1.9 and p = 0.012. Knowledge about the Pap smear, and the presence of lesions showed no association with HSV-2 infection. Multivariate analysis showed that the variable that most influenced the risk of HSV-2 infection was the presence of HIV infection, with a relative risk of 1.9 and p = 0.04. Discussion: Genital ulcers are an important entry point for HIV, and condom use is an important strategy to reduce transmission of HIV and HSV-2.

Detection of herpes simplex virus type 2 Bgl II N fragment in paraffin-embedded cervical tissue sections using nested polymerase chain reaction

1994 ◽  
Vol 8 (6) ◽  
pp. 441-447 ◽  
Author(s):  
Viraphong Lulitanond ◽  
Wasun Chantratita ◽  
Kamthorn Thammprasert ◽  
Kanchana Nimmanahaeminda ◽  
Pornchai Matangkasombut ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Cervical Tissue ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Tissue Sections ◽  
Polymerase Chain ◽  
Simplex Virus

Involvement of Herpes Simplex Virus Type 2 in Modulation of Gene Expression of Human Papillomavirus Type 18

2002 ◽  
Vol 15 (1) ◽  
pp. 59-63 ◽  
Author(s):  
S. Pisani ◽  
D. Fioriti ◽  
M. P. Conte ◽  
F. Chiarini ◽  
L. Seganti ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Human Papillomaviruses ◽  
Dna Viruses ◽  
Persistent Infections ◽  
Modulation Of Gene Expression ◽  
Simplex Virus

Human papillomaviruses (HPVs) and herpes simplex virus type 2 (HSV-2) can establish latent or persistent infections in the host, and are involved in the aetiology of benign and/or malignant lesions of the urogenital tract. To investigate the putative interaction between these DNA viruses when a double infection occurs, we have studied the effect of HSV-2 infection in HeLa 229 cells containing 10–50 copies of HPV type 18 genomic DNA. Twenty hours post HSV-2 infection, the analysis of mRNA transcripts from El, E2, E6 early and L1 late HPV18 genes was performed in HeLa cells by a semi-quantitative RT-PCR assay. A modulation of HPV18 E1 and E6 early genes was observed, resulting in a 9-fold and 3-fold increased transcription respectively.

scholarly journals Promising results from trials of vaccines against human papillomavirus (HPV) and herpes simplex virus type 2 (HSV-2) genital infections

2002 ◽  
Vol 6 (51) ◽  
Author(s):  
C M Lowndes
Keyword(s):  
England Journal ◽  
Herpes Simplex ◽  
New England ◽  
Recent Issue ◽  
Dna Viruses ◽  
Sexually Transmitted ◽  
Double Stranded Dna ◽  
Multicentre Trials ◽  
Simplex Virus

In a recent issue of the New England Journal of Medicine (1,2), two American groups published the results of multicentre trials of vaccines against genital HPV and HSV-2 infection. Both these double-stranded DNA viruses are sexually transmitted and infection can result in very serious sequelae.

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