scholarly journals Chemical Control of Excessive Vegetative Growth of Fruit Trees with Emphasis on Stone Fruit Species

Author(s):  
Amnon Erez ◽  
M.W. Williams ◽  
Yosef Ben-Tal ◽  
B. Avidan ◽  
E.A. Curry ◽  
...  
2020 ◽  
Vol 13 ◽  
pp. 76-79
Author(s):  
Alin Gheorghe ◽  
Ion Leveanu ◽  
Angela Amuza

" The stone fruits occupy an important place in the human diet due to their complex chemical composition such as sugars, free organic acids, pectic substances and vitamins. The main damage of economic importance to the stone fruit species is caused by the monilia disease caused by the fungus Monilia laxa (Aderhold et Ruhland). The disease can affect several host tissues these include blossom blight, shoot blight, fruit blight and brown fruit rot. The purpose of this research was to evaluate the attack produced by the pathogen on peach, apricot and plum from the first decade of May until the second decade of August in Experimental Field of Horticulture Faculty, USAMV Bucharest in 2019. Results showed that plums had the highest attack rate (14%) and the lowest was in apricots (0.8%). It should be noted that monilia disease is a major threat for stone fruit trees because of its aggressive manifestation on the fruit, especially in plums and peaches."


Plant Disease ◽  
2011 ◽  
Vol 95 (11) ◽  
pp. 1483-1483 ◽  
Author(s):  
H. G. Cui ◽  
N. Hong ◽  
W. X. Xu ◽  
J. F. Zhou ◽  
G. P. Wang

Plum bark necrosis and stem pitting disease was first observed on a ‘Black Beaut’ plum (Prunus salicina Lindl.) in the United States in 1986 and later is several other countries. Plum bark necrosis stem pitting-associated virus (PBNSPaV; genus Ampelovirus, family Closteroviridae), the putative causal agent of the disease, infects many stone fruit species and causes decline, gummosis, flattening of scaffold branches, and stem necrotic pits in some diseased trees (1,3). An investigation of the incidence of PBNSPaV on stone fruit trees in China was conducted during 2009 and 2010. Leaf samples were collected from 47 trees, including peach (P. persica L. Batsch), nectarine (P. persica L. var. nucipersica Schneider), plum (P. domestica L.), ornamental plum (P. cerasifera Ehrb), sweet cherry (P. avium L.), and flowering cherry (P. serrulata L.), grown in Hubei, Henan, and Shandong provinces in central and northern China. Most of sampled trees showed trunk gummosis or stem pitting. The presence of PBNSPaV was tested by reverse transcription (RT)-PCR using primer set PBN195F/PBN195R (5′-CTGGTCTTCCTGCTACTCCTT-3′/5′-AAGCCCACAATCTCAGAGCG-3′) designed for the detection of the coat protein (CP) gene of the virus. Total RNA was extracted from leaves using a CTAB protocol reported by Li et al. (2). Products of the expected size of 190 bp were amplified from 20 samples, including seven cultivated peach, four ornamental peach, one nectarine, two plum, one ornamental plum, three sweet cherry, and two flowering cherry samples. All trees positive for PBNSPaV showed stem pitting symptoms on the base of the trunk. To further confirm these results, a 590-base region of the heat shock protein 70 homolog (HSP70h) gene was amplified by RT-PCR using primers HSP-P1/HSP-P2 (5′-GGAATTGACTTCGGTACAAC-3′/5′-TCGAAAGTACCACCACCGAA-3′). Amplicons of the expected size were cloned into the vector pMD18-T (TaKaRa, Dalian, China) and sequenced by Genscript Corp. (Nanjing, China). Sequences of 18 PBNSPaV isolates were deposited in GenBank with Accession Nos. JF810177–JF810194. Sequence comparisons showed that the partial HSP70h gene from the Chinese PBNSPaV isolates shared 82.2 to 100% nucleotide (nt) and 94.0 to 100% amino acid (aa) similarities between them and 83.6 to 99.1% nt and 94 to 100% aa similarities with the corresponding region of the other PBNSPaV isolates deposited in GenBank. In July 2010, peach GF305 seedlings were inoculated by side grafting with budwoods from two PBNSPaV-positive ornamental peach plants. In June 2011, grooving symptom was observed on the stems of the seedlings and the virus was detected by RT-PCR. The results further confirmed PBNSPaV infection in China. These results show that PBNSPaV and the associated disease occur in main cultivated and ornamental Prunus species in China. Given the importance and the devastating symptoms of the disease, it is important to prevent virus spread by using virus-tested propagation materials. References: (1) M. Al Rwahnih et al. Arch. Virol. 152:2197, 2007. (2) R. Li et al. J. Virol. Methods 154:48, 2008. (3) D. B. Marini et al. Plant Dis. 86:415, 2002.


Hilgardia ◽  
1933 ◽  
Vol 8 (3) ◽  
pp. 83-123 ◽  
Author(s):  
Edward E. Wilson

Author(s):  
V. P. Hayova

Abstract A description is provided for Leucostoma cinctum. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. DISEASE: Leucostoma cinctum, especially in its conidial state, is a well-known pathogen of stone-fruit trees causing necrosis of twigs, perennial Cytospora-canker. The fungus penetrates mainly through the scars, and may result in dieback of branches or even whole trees. Tree susceptibility to L. cinctum is influenced by lesions (Stanova, 1990). Comparative anatomy and host response of peach cultivars inoculated with L. cinctum was studied by Biggs (1986). Resistance of different cultivars of stone-fruit trees to L cinctum has been investigated by many authors (Cociu et al., 1991; Miles et al., 1989; Pedryc & Rozsnyai, 1991). HOSTS: On dead or dying, attached or fallen twigs of the Rosaceae, mainly Prunoideae (Amygdalus, Armeniaca, Cerasus, Persica, Prunus) and rarely other subfamilies of the Rosaceae, including genera such as Cotoneaster, Crataegus, Malus and Pyrus. GEOGRAPHICAL DISTRIBUTION: Asia: Armenia, Republic of Georgia, Iran, Kazakhstan, Russia, Turkmenistan, Uzbekistan. Australasia: Australia. Europe: Czech Republic, France, Germany, Hungary, Italy, Moldova, Rumania, Russia, Slovakia, Spain, Switzerland, Sweden, Turkey, UK, Ukraine, former Yugoslavia. North America: Canada, USA (Idaho, Michigan, New-Jersey, Oregon). TRANSMISSION: Both conidia and ascospores are air-borne, especially under humid conditions. Orange or reddish droplets or tendrils of conidia extruded from conidiomata can be often seen after rain. It is also known that arthropods can carry propagules in stone-fruit orchards (Helton et al., 1988).


Plant Disease ◽  
2020 ◽  
Author(s):  
Rachid Tahzima ◽  
Radouane Qessaoui ◽  
Yoika Foucart ◽  
Sebastian Massart ◽  
Kris De Jonghe

Plum (Prunus domestica L., Rosaceae) trees, like many stone fruit trees, are known to be infected by numerous plant viruses, predominantly as consequence of their clonal mode of propagation and perennial cultivation (Jelkmann and Eastwell, 2011). Apricot vein clearing-associated virus (AVCaV) is a member of the genus Prunevirus in the family Betaflexiviridae. AVCaV was first reported in Italy infecting apricot (P. armeniaca L.) associated with foliar vein clearing symptoms (Elbeaino et al. 2014). It has also been detected in various Prunus species, like plum, Japanese plum (P. salicina L.), sour cherry (P. cerasus L.), and Japanese apricot (P. mume L.), apricot and peach (P. persica L.) sourced from Asian and European countries (Marais et al. 2015), as well as in the ornamental Myrobolan plum (P. cerasifera L.) in Australia (Kinoti et al. 2017). In 2018, during the vegetative season, a survey was carried out in two different apricot and plum orchards in the southern region of Agdez (Agadir, Morocco) where stone fruit trees are grown. Five branches with leaves were sampled from three apricot and three plum trees of unknown cultivars, all asymptomatic. Total RNA was extracted from 100 mg plant tissue (leaves and cambial scrapping) using RNeasy Plant Mini Kit (QIAGEN, Hilden, Germany) and separate samples (one per species) were used for library preparation (NEBNext Ultra RNA library kit; New England BioLabs, MA, USA), and sequencing (Illumina NextSeq v2, totRNA sequencing) at Admera Health (New Jersey, USA). All generated reads (6,756,881) from the plum sample were quality filtered and submitted to the VirusDetect pipeline (Zheng et al., 2017). The plum cDNA library, a total of 20 viral contigs (68-1928 bp) mapped to several AVCaV accessions in GenBank. A reference mapping (CLC Genomics Workbench 12, Qiagen, Denmark) was conducted against all four available AVCaV full genomes (KM507062-63, KY132099 and HG008921), revealing 100% coverage of the full sequence (8358 nt) with 97-98 % nucleotide (nt) identities (BLASTn). Analysis of the derived sequences allowed to identify the location of the four predicted ORFs i.e. (ORF1: 6066 nt/2,021 aa), (ORF2: 1383 nt/460 aa), (ORF3: 666 nt/221 aa) and (ORF4: 420 nt/139 aa), previously described for the AVCaV genome (Elbeaino et al. 2014). The amino acid sequences of the encoded proteins of AVCaV isolate from Morocco also shared 97-98% identities with the corresponding sequences of complete genome AVCaV isolates in GenBank. To confirm the detection of AVCaV in the three plum samples, specific RT-PCR primers (VC37657s: 5’-CCATAGCCACCCTTTTTCAA-3’ / VC28239a: 5’-GTCGTCAAGGGTCCAGTGAT-3’) (Elbeaino et al. 2014) were used and the expected 330 bp fragment from the replicase gene was amplified in all three samples and subsequently sequenced (MT980794-96). Sanger sequences were 100% identical to corresponding HTS derived sequence. This is the first report of AVCaV infecting plum in Africa. The incidence of AVCaV in Moroccan Prunus species is unknown. Plum trees from the surveyed orchards were also confirmed to be co-infected with little cherry virus 1 (LChV-1) using HTS. Further investigation is required to determine the impact of AVCaV on these asymptomatic plum trees and other stone fruits species.


2010 ◽  
pp. 34-41
Author(s):  
Gábor Tarcali ◽  
Emese Kiss ◽  
György J. Kövics ◽  
Sándor Süle ◽  
László Irinyi ◽  
...  

Plant diseases caused by phytoplasmas have increasing importance in all over the world for fruit growers. Lately, phytoplasma diseases occur on many fruit varieties and responsible for serious losses both in quality and quantity of fruit production. In the long-run these diseases cause destruction of fruit trees. The apricot phytoplasma disease (Ca. Phytoplasma prunorum) was first reported in Europe in 1924 from France. In 1992 the disease has also been identified in Hungary. On the base of growers' signals serious damages of "Candidatus Phytoplasma prunorum" Seemüller and Schneider, 2004 (formerly: European stone fruit yellows phytoplasma) could be observed in different stone fruit plantations in the famous apricot-growing area nearby Gönc town, Northern-Hungary. Field examinations have been begun in 2009 in several stone fruit plantations in Borsod-Abaúj-Zemplén County mainly in Gönc region which is one of the most important apricot growing regions in Hungary, named “Gönc Apricot Growing Area”. Our goals were to diagnose the occurrence of Ca. Phytoplasma prunorum on stone fruits (especially on apricot) in the North-Hungarian growing areas by visual diagnostics and confirm data by laboratory PCR-based examinations. All the 28 collected samples were tested in laboratory trials and at 13 samples from apricot, peach, sour cherry and wild plum were confirmed the presence of phytoplasma (ESFY). On the base of observations it seems evident that the notable losses caused by "Ca. Phytoplasma prunorum" is a new plant health problem to manage for fruit growers, especially apricot producers in Hungary. 


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