scholarly journals Resistance inducers and organic fertilizer in Citrus sinensis [L.] Osbeck infected with Candidatus Liberibacter asiaticus bacteria

2021 ◽  
Author(s):  
Yazmin Zapata Contreras ◽  
Eduardo Osorio Hernandez ◽  
José Hugo Silva Espinosa ◽  
Criseida Alhelí Saénz Pérez ◽  
Ma. Teresa de Jesús Segura Martínez
Keyword(s):  
Citrus Sinensis ◽  
Organic Fertilizer ◽  
Fruit Weight ◽  
Skin Thickness ◽  
Candidatus Liberibacter Asiaticus ◽  
Equatorial Diameter ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus ◽  
Chlorophyll Index ◽  
Resistance Inducers

Objective: To evaluate the effect of three resistance inducers and an organic fertilizer on the titles of CandidatusLiberibacter asiaticus in Citrus sinensis (L.) Obseck cv. Valencia.Design/methodology/approach: The treatments consisted of Vacciplant Max and UPL-08, Fosetil aluminum. Likewise, BIO-FOM was applied on the periphery of the trees, with moisture for nutrients absorption. The evaluated variables were fruit weight, equatorial diameter, skin thickness, °BRIX, severity and chlorophyll, in each of the five treatments, which consisted of 20 repetitions.Results: The fruits of the trees treated with Vacciplant Max had lower skin thickness and a higher °BRIX. Also, the highest chlorophyll index was recorded with BIO-FOM fertilizer. However, none of the evaluated treatments significantly decreased the fruit harshness.Findings/conclusions: The best treatment against Huanglongbing was fosetyl aluminum which conferred greater fruit weight and diameter

scholarly journals Multi-omics Comparison Reveals Landscape of Citrus limon and Citrus sinensis Response to ‘Candidatus Liberibacter asiaticus’

2021 ◽  
pp. PHYTOFR-09-20-0
Author(s):  
Elizabeth L. Chin ◽  
John Ramsey ◽  
Surya Saha ◽  
Darya Mishchuk ◽  
Juan Chavez ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Citrus Sinensis ◽  
Mass Spectrometry Analysis ◽  
Citrus Limon ◽  
Symptom Development ◽  
Candidatus Liberibacter Asiaticus ◽  
Spectrometry Analysis ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus

Comparison of the metabolic changes prior to symptom development upon infection with Candidatus Liberibacter asiaticus (CLas), the bacterium associated with citrus greening disease, between citrus hosts with different tolerances is lacking. The objective of this study was to compare the early response of Lisbon lemon (Citrus limon) and Washington navel orange (Citrus sinensis [L.] Osbeck), two citrus species commercially important to California, to CLas through graft inoculation. Here, we compare the transcriptome, proteome, and metabolome response, using RNA sequencing, liquid chromatography tandem mass spectrometry, and 1H nuclear magnetic resonance, respectively, from our two recently published studies examining the response of the lemon and navel oranges separately, and introduce new micronutrient data from inductively coupled plasma mass spectrometry analysis, focusing on lemons at 10 and 14 weeks postgrafting (wpg), and navels at 8 and 18 wpg, prior to symptom development. Several micronutrients accumulated in presymptomatic infected lemons compared with controls, whereas little change was observed in the navels. Photosynthesis proteins were substantially altered by CLas infection in navels, with fewer changes observed in lemons. The metabolome differed between control and infected navels throughout infection, although differences between control and infected lemons were identified only after symptom expression. Taken together, these findings highlight differences in response to CLas between two varieties with differing tolerances. [Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

scholarly journals PHLOEM PROMOTERS IN TRANSGENIC SWEET ORANGE ARE DIFFERENTIALLY TRIGGERED BY Candidatus Liberibacter asiaticus

2017 ◽  
Vol 39 (3) ◽  
Author(s):  
LUZIA YURIKO MIYATA ◽  
RICARDO HARAKAVA ◽  
LÍSIA BORGES ATTÍLIO ◽  
BEATRIZ MADALENA JANUZZI MENDES ◽  
JOÃO ROBERTO SPOTTI LOPES ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Citrus Sinensis ◽  
Transgene Expression ◽  
Sweet Orange ◽  
Candidatus Liberibacter Asiaticus ◽  
Specific Expression ◽  
Gus Reporter ◽  
Uida Expression ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus

ABSTRACT The use of promoters preferentially expressed in specific plant tissues is a desirable strategy to search for resistance for pathogens that colonize these tissues. The bacterium Candidatus Liberibacter asiaticus (Las), associated with huanglongbing disease (HLB) of citrus, colonizes phloem vessels. Some promoters, besides conferring tissue-specific expression, can also respond to the presence of the pathogen. The objective of the present study was to verify if the presence of Las could modulate the activation of the phloem-specific promoters AtPP2 (Arabidopsis thaliana phloem protein 2), AtSUC2 (A. thaliana sucrose transporter 2) and CsPP2 ( pCitrus phloemrotein 2), known to be expressed in Citrus sinensis phloem. ‘Hamlin’ sweet orange plants (Citrus sinensis L. Osbeck) transformed with the uidA (GUS) reporter gene under the control of AtPP2, AtSUC2 and CsPP2 promoters were infected to evaluate the interdependence between transgene expression and the concentration of Las. Plants were inoculated with Las by Diaphorina citri and eighteen months later, bacterial concentration and uidA expression were determined by qPCR and RT-qPCR, respectively. Reporter gene expression driven by AtSUC2 promoter was strongly and positively correlated with Las concentration. Therefore, this promoter combines desirable features of both tissue-specificity and pathogen-inducibility for the production of transgenic plants tolerant to Las.

scholarly journals Metabolomic Response to Huanglongbing: Role of Carboxylic Compounds in Citrus sinensis Response to ‘Candidatus Liberibacter asiaticus’ and Its Vector, Diaphorina citri

2017 ◽  
Vol 30 (8) ◽  
pp. 666-678 ◽  
Author(s):  
Nabil Killiny ◽  
Yasser Nehela
Keyword(s):  
Gene Expression ◽  
Amino Acids ◽  
Fatty Acids ◽  
Expression Analysis ◽  
Citrus Sinensis ◽  
Gene Expression Analysis ◽  
Diaphorina Citri ◽  
Candidatus Liberibacter Asiaticus ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus

Huanglongbing, a destructive disease of citrus, is caused by the fastidious bacterium ‘Candidatus Liberibacter asiaticus’ and transmitted by Asian citrus psyllid, Diaphorina citri. The impact of ‘Ca. L. asiaticus’ infection or D. citri infestation on Valencia sweet orange (Citrus sinensis) leaf metabolites was investigated using gas chromatography mass spectrometry, followed by gene expression analysis for 37 genes involved in jasmonic acid (JA), salicylic acid (SA), and proline-glutamine pathways. The total amino acid abundance increased after ‘Ca. L. asiaticus’ infection, while the total fatty acids increased dramatically after infestation with D. citri, compared with control plants. Seven amino acids (glycine, l-isoleucine, l-phenylalanine, l-proline, l-serine, l-threonine, and l-tryptophan) and five organic acids (benzoic acid, citric acid, fumaric acid, SA, and succinic acid) increased in ‘Ca. L. asiaticus’-infected plants. On the other hand, the abundance of trans-JA and its precursor α-linolenic increased in D. citri-infested plants. Surprisingly, the double attack of both D. citri infestation and ‘Ca. L. asiaticus’ infection moderated the metabolic changes in all chemical classes studied. In addition, the gene expression analysis supported these results. Based on these findings, we suggest that, although amino acids such as phenylalanine are involved in citrus defense against ‘Ca. L. asiaticus’ infection through the activation of an SA-mediated pathway, fatty acids, especially α-linolenic acid, are involved in defense against D. citri infestation via the induction of a JA-mediated pathway.

scholarly journals Comparación de enzimas y compuesto fenólicos, en tres especies de cítricos infectadas por Candidatus Liberibacter asiaticus

2017 ◽  
Vol 35 (2) ◽  
Author(s):  
Lidia Monserrat Flores Torres ◽  
Alberto Flores Olivas ◽  
Yisa María Ochoa Fuentes ◽  
José Isabel López Arroyo ◽  
Víctor Olalde Portugal ◽  
...  
Keyword(s):  
Citrus Sinensis ◽  
Candidatus Liberibacter Asiaticus ◽  
Citrus Aurantifolia ◽  
Citrus Latifolia ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus

El objetivo fue determinar diferencias en la actividad enzimática antioxidante y la concentración de fenoles totales en plantas de limón mexicano (Citrus aurantifolia (Christm.) Swingle), limón persa (Citrus latifolia Tanaka); y naranja dulce Valencia (Citrus sinensis (L.) Osbeck), infectadas bajo condiciones naturales de campo con Candidatus Liberibacter asiaticus (CaLas), en Bustamante, Tamaulipas, México. Se determinó la presencia de CaLas por qPCR; la actividad enzimática de fenilalanina amonio liasa (PAL), que se activa ante el ataque de patógenos; peroxidasa que interviene en la lignificación de paredes como respuesta a la infección; α-amilasa que degrada el almidón y se acumula en el tejido vascular, y compuestos fenólicos que cumplen funciones de defensa. Se realizó un análisis de contrastes. La concentración de proteínas totales mostró diferencias significativas entre especies entre especies (P&lt; 0.0001). Con una media de 6.1 y 6.37 mg de proteínas por g de tejido fresco para árboles infectados de limón mexicano; y naranja dulce negativa a CaLas. La actividad peroxidasa presentó diferencia significativa para limón persa (P=0.0341), con una media de 1.96 U.mg de proteína-<sup>1</sup>. En naranja dulce se observó mayor actividad de α amilasa en los árboles infectados por CaLas (1.19 U.mg de proteína-<sup>1</sup>). La concentración de PAL y fenoles totales no presentaron diferencias significativas entre especies. En el presente estudio se observó que CaLas influye en la actividad enzimática de cítricos.

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