scholarly journals IN VITRO SCREENING FOR ANTIMICROBIAL POTENTIAL OF ETHANOLIC LEAF EXTRACTS OF SOME BEGONIA SPECIES AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) STRAIN

2020 ◽  
pp. 30-38
Author(s):  
Halyna Tkachenko ◽  
Natalia Kurhaluk ◽  
Lyudmyla Buyun ◽  
Vladimir Tomin
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Inhibition Zone ◽  
Leaf Extracts ◽  
Test Protocol ◽  
Methicillin Resistant ◽  
Highly Active ◽  
Ethanolic Extracts

The antimicrobial activities of ethanolic extracts obtained from the leaves of Begonia solimutata L.B. Sm. & Wassh., Begonia goegoensis N.E.Br., Begonia foliosa Kunth, Begonia × erythrophylla Hérincq, Begonia thiemei C.DC., Begonia peltata Otto & Dietr., Begonia heracleifolia Cham. & Schltdl., Begonia dregei Otto & Dietr., and Begonia mexicana G. Karst. ex Fotsch were assessed against methicillin-resistant Staphylococcus aureus (MRSA) strain. The leaves were brought into the laboratory for antimicrobial studies. Freshly crushed leaves were washed, weighed, and homogenized in 96% ethanol (in proportion 1:19) at room temperature. The extracts were then filtered and investigated for their antimicrobial activity. The testing of the antibacterial activity of the plant extracts was carried out in vitro by the Kirby-Bauer disc diffusion susceptibility test protocol. The most effective plants among species screened against S. aureus NCTC 12493 growth were B. peltata, B. thiemei, B. foliosa, Begonia × erythrophylla, and B. solimutata being highly active with the ethanolic extracts (diameters of inhibition zone ranged from 12.5 to 21 mm). The ethanolic extracts from nine Begonia plant species were evaluated for their antimicrobial activity against methicillin-resistant S. aureus (MRSA) strain. Extracts from all test Begonia plants caused a remarkable reduction in bacterial growth, measured as an inhibition zone diameters. The diameter of the inhibition zone for B. solimutata was (16.4±1.1) mm, for B. goegoensis – (14.2±1.0) mm, for B. foliosa – (14.9±1.2) mm, for Begonia × erythrophylla – (16.9±0.9) mm, for B. thiemei – (16.8±1.3) mm, for B. peltata – (18.1±0.9) mm, for B. heracleifolia – (15.3±0.9) mm, for B. dregei – (14.7±1.1) mm, and for B. mexicana – (13.8±0.9) mm/ The highly active antimicrobial effects of extracts obtained from B. peltata and B. thiemei noted against S. aureus NCTC 12493 growth is worthy of highlighting. Our studies indicated that Begonia plants are worthy of further investigation as a potential phytotherapeutic agent for treating infections caused by S. aureus., as well as for the development of innovative feed and preventative products in animal husbandry.

scholarly journals In Vitro Antimicrobial Activity of Bawang Batak Bulbs (Allium Chinense G. Don) Against Methicillin-Resistant Staphylococcus Aureus

2019 ◽  
Vol 2 (2) ◽  
pp. 67-71
Author(s):  
Tiasarah Aretha ◽  
Nelva Karmila Jusuf
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Alternative Therapy ◽  
Inhibition Zone ◽  
Negative Control ◽  
Diffusion Method ◽  
Methicillin Resistant ◽  
Allium Chinense ◽  
Concentration Levels

Introduction: Staphylococcus aureus infection can cause pyoderma. These days there is a shift in Staphylococcus aureus sensitivity to antibiotics that is marked by the existence of Methicillin-Resistant Staphyloccus aureus, so alternative therapy that is herbal medicine might be needed. There are a lot of medicinal plants in Indonesia, but until today the usage is still minimum. One of the plant genus that is already being used is allium that had been known to have antimicrobial activity. The genus allium that is consumed a lot by Indonesians especially people of North Sumatra is bawang batak (Allium chinense G.Don.). This study is conducted to assess the antimicrobial activity of bawang batak bulbs in several concentrations to MRSA. Methods: This research is an experimental research with agar diffusion method. The extracts were divided into six groups: extracts in concentration level of 6.25%, 12.5%, 25%, 50%, 100% and a negative control (DMSO). The sample size is four per group with total sample of 24. Results: The results showed that the inhibition of bacterial growth occured at the concentration levels of 50% and 100% with inhibition zone diameters of 8.695 mm and 10.545 mm respectively. Extracts with concentration levels of 25%, 12.5% and 6.25% did not show any inhibition. Conclusion: Bawang batak bulbs extract can be used to inhibit the growth of MRSA

scholarly journals Susceptibility Assessment of Methicillin-Resistant Staphylococcus aureus Strains to Lepidium sativum Extract

Dose-Response ◽  
2019 ◽  
Vol 17 (2) ◽  
pp. 155932581985042
Author(s):  
Amna A. Saddiq ◽  
Azza M. Mohamed
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Exposure Period ◽  
Inhibition Zone ◽  
Lepidium Sativum ◽  
Susceptibility Assessment ◽  
Methicillin Resistant ◽  
Bacteria Growth ◽  
Microbial Infections

Many plant-derived compounds have been used to treat microbial infections. Staphylococcus aureus a common cause of many organ infections, has generated increasing concern due to its resistance to antibacterial drugs. This work was carried out to explore the susceptibility of 6 strains (LN872136, LN872137, LN871238, LN871239, LN872140, and LN871241) of methicillin-resistant Staphylococcus aureus to aqueous extract of Lepidium sativum seeds in vitro. Various concentrations (5-20 mg/mL) were used to evaluate the effect of the extract on bacteria growth via the assessment of the microbial biomass and the inhibition zone (IZ). The results showed that the plant extract at 15 or 20 mg/mL, significantly decreased the the biomass of S aureus strains after 24 or 48 hours exposure period. Staphylococcus aureus (LN871241) showed the largest IZ at 20 mg/mL and documented by scanning electron microscope. The current work may suggest that L sativum seed extract can be candidate as a promising antimicrobial agent to treat infection with methicillin-resistant S aureus.

scholarly journals Chloride anion transporters inhibit growth of methicillin-resistant Staphylococcus aureus (MRSA) in vitro

2016 ◽  
Vol 52 (48) ◽  
pp. 7560-7563 ◽  
Author(s):  
Andrew I. Share ◽  
Khushali Patel ◽  
Cristina Nativi ◽  
Eun J. Cho ◽  
Oscar Francesconi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Chloride Anion ◽  
Methicillin Resistant ◽  
Anion Transporters

Anion transporters were tested for antimicrobial activity and shown to inhibit the growth of MRSA in vitro.

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