scholarly journals Analysis of Total Terpenoids from Maniltoa Grandiflora (A. Gray) Scheff Leaves Using TLC and HPLC Methods

2020 ◽  
Vol 2 (2) ◽  
pp. 5-9
Author(s):  
Jhon Patar Sinurat ◽  
Visensius Krisdianilo ◽  
Reh Malem br Karo ◽  
Rinaldo Berutu
Keyword(s):  
Phosphoric Acid ◽  
Ethyl Acetate ◽  
Flow Rate ◽  
Liquid Extraction ◽  
Liquid Liquid Extraction ◽  
Tlc Analysis ◽  
Partition Method

Terpenoids screening were carried out using Liebermann Burchard and Salkowski reagent on the extract of Saputangan leaves. It showed that the leaves contained terpenoid compounds with appeared of a reddish brown ring in the extract and a reddish brown stain appeared on the TLC plate tested with 1% CeSO4 reagent in 10% H2SO4. The macerate of saputangan leaves processed separation using the partition method (Liquid-liquid Extraction). Extracts dissolved with methanol were partitioned with n-Hexane and then partitioned between aquadest and ethyl acetate in a ratio of 1: 1 to obtain 50 g of total terpenoids. Furthermore, TLC analysis was performed on total terpenoids using n-hexane: ethyl acetate (80:20 v/v) solvent to obtain 11 separate stains on the TLC plate with different Rf each. Analysis was enhanced in HPLC using 100% acetonitrile and 0.1% phosphoric acid at a wavelength of 210 nm, a flow rate of 0.500 mL/min and eluted for 30 minutes. Based on the HPLC results, there were 25 peaks which indicated the presence of total terpenoid compounds with the highest peak being peak no. 8 (ret.time's 6.234, area's 8503532 and height's 276032), peak no. 9 (ret.time's 6.674, area's 3322572 and height's 141859) and peak no. 10 (ret.time's 7.288, area's 2758231 and height's 103927)

scholarly journals Identification of Phytochemicals from the Water Extract of Eurycoma longifolia Roots using Solid-Liquid and Liquid-Liquid Extraction Based Fractionation Techniques

2021 ◽  
Vol 68 (4) ◽  
pp. 765-772
Author(s):  
Lee Suan Chua ◽  
Abirame Segaran ◽  
Hoi Jin Wong
Keyword(s):  
Ethyl Acetate ◽  
Solid Phase ◽  
Water Extract ◽  
Reversed Phase ◽  
Liquid Extraction ◽  
Acetone Precipitation ◽  
Eurycoma Longifolia ◽  
Liquid Liquid Extraction ◽  
Saponin Content ◽  
Solid Liquid

Phytochemicals in the water extract of Eurycoma longofolia roots were identified using both solid-liquid and liquid-liquid extraction based fractionation techniques. A reversed phase C18 solid phase extraction (SPE) was used as solid-liquid extraction, whereas solvent partition was applied as liquid-liquid extraction. Total saponin was increased after fractionation. A few known quassinoids; eurycomanone, 13α(21)-epoxyeurycomanone, pasakbumin D, 13β,18-dihydroeurycomanol and 13β,21-dihydroxyeurycomanol were identified from the 40% and 60% methanol fractions of SPE. Solvent partition extract using ethyl acetate was found to have the highest saponin content compared to butanol and chloroform fractions. Subsequent acetone precipitation of the organic fractions recovered a formylated hexose trimer and other saccharide-containing compounds. Ethyl acetate effectively recovered saponins from E. longofolia water extract using liquid-liquid extraction followed by acetone precipitation.

scholarly journals Comparison of two sample preparation procedures for HPLC determination of ochratoxin A

2009 ◽  
Vol 61 (4) ◽  
pp. 639-644 ◽  
Author(s):  
Gorica Vukovic ◽  
Snezana Pavlovic ◽  
M.S. Ristic
Keyword(s):  
Sample Preparation ◽  
Phosphoric Acid ◽  
Ochratoxin A ◽  
Chromatographic Determination ◽  
Liquid Extraction ◽  
Immunoaffinity Column ◽  
Maize Flour ◽  
Liquid Liquid Extraction ◽  
Immunoaffinity Columns

In preparation of samples for chromatographic determination of ochratoxin A, two types of columns were used for sample cleanup (SPE and immunoaffinity columns). The first method consisted of liquid-liquid extraction with a mixture of chloroform and phosphoric acid, followed by ion-exchange cleanup on Waters Oasis MAX columns. The sec?ond method consisted of extraction with a mixture of water and methanol, followed by LCTech OtaCLEAN immunoaf?finity column cleanup. Recoveries of the methods were determined at three levels in three repetitions for maize flour, and they were 84% (%RSD = 19.2) for the first method of sample preparation and 101% (%RSD = 2.2) for the second method. Values of LOQ for OTA were 0.25 and 1.00 ?g/kg for the IAC and SPE clean-up procedures, respectively. Both methods comply with present regulations, but the MAX sample clean-up procedure should be used as an alternative, since the immunoaffinity column clean-up procedure is characterized by better reproducibility, accuracy, and efficiency.

scholarly journals Determination of the Trans-resveratrol content of Champagne wines by reversed-phase HPLC

OENO One ◽  
2006 ◽  
Vol 40 (2) ◽  
pp. 117 ◽  
Author(s):  
Philippe Jeandet ◽  
David Chaudruc ◽  
Bertrand Robillard ◽  
F. Peters ◽  
Dominique Tusseau ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Reversed Phase ◽  
Liquid Extraction ◽  
Fluorometric Detection ◽  
Reversed Phase Hplc ◽  
Liquid Liquid Extraction

<p style="text-align: justify;">Levels of trans-resveratrol in Champagne wines were determined by the use of reversed-phase HPLC with UV and fluorometric detection after liquid-liquid extraction with ethyl acetate. Resveratrol concentrations in Champagne wines range from 20 to 77 μg/L except for the Champagne rosé in which resveratrol reaches several hundred micrograms per litre. The resveratrol content of Champagne wines was also shown to decrease with aging on lees.</p>

Use of combined solvent extraction-electrodeposition techniques to uranium and thorium isotopes analysis in industrial phosphoric acid, phosphate and phosphogypsum by alpha-particle spectrometry

2003 ◽  
Vol 91 (1) ◽  
Author(s):  
R. Zarki ◽  
A. Elyahyaoui ◽  
A. Chiadli
Keyword(s):  
Solvent Extraction ◽  
Phosphoric Acid ◽  
Isotopic Composition ◽  
Alpha Particle ◽  
Phosphate Rock ◽  
Liquid Extraction ◽  
Acid Phosphate ◽  
Liquid Liquid Extraction ◽  
Thorium Isotopes

SummaryA method combining liquid-liquid extraction and electrodeposition procedures is carried out to determine isotopic composition of uranium and thorium in technical wet phosphoric acid, phosphate rock and phosphogypsum leachates, by

scholarly journals Liquid-liquid extraction technique for purification of Egyptian WET process phosphoric acid

2010 ◽  
Vol 54 (2) ◽  
pp. 57 ◽  
Author(s):  
Ali Akl Magda ◽  
Mohammed Youssef Abdul Fatah ◽  
Mohammed Ali Mohsen ◽  
Ibraheim Amin Mostafa
Keyword(s):  
Phosphoric Acid ◽  
Liquid Extraction ◽  
Extraction Technique ◽  
Wet Process ◽  
Liquid Liquid Extraction

scholarly journals Identification of Transient Receptor Potential Vanilloid 3 Antagonists from Achillea alpina L. and Separation by Liquid-Liquid-Refining Extraction and High-Speed Counter-Current Chromatography

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2025 ◽  
Author(s):  
Shi-Wei Sun ◽  
Rong-Rong Wang ◽  
Xiao-Ying Sun ◽  
Jia-He Fan ◽  
Hang Qi ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
High Speed ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Ethanol Extract ◽  
Liquid Extraction ◽  
Transient Receptor Potential Vanilloid ◽  
Liquid Liquid Extraction ◽  
Transient Receptor ◽  
Counter Current

Bioassay-guided fractionation of the ethanol extract of whole herbs of Achillea alpina led to the isolation of isochlorogenic acids A and B as transient receptor potential vanilloid 3 (TRPV3) channel antagonists by using a calcium fluorescent assay. The structures were identified by spectroscopic analysis and the inhibitory activities of isochlorogenic acids A and B were confirmed by whole-cell patch clamp recordings of human embryonic kidney 293 (HEK293) cells expressing human TRPV3. Molecular docking results revealed that these two compounds reside in the same active pocket of human TRPV3 channel protein with lower binding energy than the agonist 2-aminoethoxydiphenyl borate (2-APB). High-speed counter-current chromatography (HSCCC) coupled with a liquid-liquid extraction approach was successfully established for the separation of isochlorogenic acids A and B from the whole herbs of A. alpina. Ethyl acetate and n-hexane-ethyl acetate-water (3:3:4 and 1:5:4, v/v/v) were selected as liquid-liquid extraction solvent systems to remove high- and low-polarity impurities in the mixture. Sixty g of ethanol extract was refined by solvent partition to yield 1.7 g of the enriched fraction, of which 480 mg in turn obtained 52.5 mg of isochlorogenic acid B (purity 98.3%) and 37.6 mg isochlorogenic acid A (purity 96.2%) after HSCCC with n-hexane-ethyl acetate-water containing 1% acetic acid (1:4:8, v/v/v).

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