ASSESSMENT OF QUALITATIVE CHANGES IN PERIPHERAL BLOOD CELLS IN CHILDREN – RESIDENTS OF RADIOLOGICALLY CONTAMINATED TERRITORIES IN THE LATE PERIOD AFTER THE ChNPP ACCIDENT

Objective: to establish the relationship between quantitative and qualitative parameters of peripheral blood cells (lymphocytes, neutrophilic granulocytes, monocytes, platelets) depending on the type of somatic diseases and annual internal radiation doses from 137Cs in children – residents of radiologically contaminated territories in the late period after the Chornobyl Nuclear Power Plant (ChNPP) accident. Materials and methods. There were 175 children included in the study comprising residents of radiologically contaminated territories (n = 79) aged from 4 to 18 years. Annual internal radiation doses in children from 137Cs ranged from 0.004 to 0.067 mSv. Certain blood parameters were assessed in a comparative mode in children having got the radiation doses up to 0.01 mSv and higher. The comparison group (n = 96) included children living in settlements not attributed to the radiologically contaminated ones. Incidence and type of somatic diseases and its impact on quantitative and qualitative changes in blood parameters (i.e. lymphocyte, neutrophilic granulocyte, monocyte, and platelet count) were studied. The cell size, state of nucleus, membranes and cytoplasm, signs of proliferative and degenerative processes were taken into account. Results. Incidence and type of somatic diseases in children did not depend on the annual internal radiation dose. Number of cases of monocytosis was significantly higher among the children exposed to ionizing radiation than in the comparison group (16.6 % vs. 7.3 %). There were, however, no correlation between these changes and radiation doses. Number of activated blood monocytes with cytoplasmic basophilia and residues of nucleoli in nuclei was higher in individuals with internal radiation doses > 0.01 mSv. A direct correlation between the qualitative parameters of monocytes and internal radiation doses was established (rs = 0.60; р < 0.001), as well as a direct correlation of different strength between qualitative parameters of blood cells, indicating their unidirectional pattern depending on the somatic morbid conditions. Regardless of annual internal radiation dose, there was an increase in the number of degenerative and aberrant cells vs. the comparison group (р < 0.05), which could be due to the role of non-radiation factors. Conclusions. Results of the assessment of quantitative and qualitative parameters of peripheral blood cells reflected the state of morbid conditions in children and are of a diagnostic value. The identified dose-dependent changes in monocyte lineage of hematopoiesis may be the markers of impact of long-term radionuclide incorporation with food in children living in environmentally unfavorable conditions after the ChNPP accident. Key words: annual internal radiation dose from 137Cs, children, peripheral blood, lymphocytes, neutrophilic granulocytes, monocytes, platelets, qualitative signs.

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In vitro effects of platelet-derived factors of brain glioma patients on C6 glioma cells

Regulatory Mechanisms in Biosystems ◽

10.15421/021928 ◽

2019 ◽

Vol 10 (2) ◽

pp. 187-196

Author(s):

L. D. Liubich ◽

N. I. Lisyanyi ◽

T. A. Malysheva ◽

L. P. Staino ◽

D. M. Egorova ◽

...

Keyword(s):

Mitotic Activity ◽

Peripheral Blood ◽

Blood Cells ◽

Comparison Group ◽

Tumour Cells ◽

Peripheral Blood Cells ◽

Brain Glioma ◽

Cell Clusters ◽

Glioma C6

Platelets play an important part in the progression and pathological angiogenesis of brain glioma because of the different granules content and release of microvesicles that are the source of numerous mediators and bioactive substances, which probably provides a "strategy" for the tumour survival. The objective of study was exploring the effect of platelet-released secretion products of patients with brain glioma on the experimental model of tumour growth in vitro. For this purpose, the cells of glioma C6 were cultured for 72 hours under the addition of modified media containing platelet-released secretion products or conditioned media of peripheral blood cells of patients with glioma as well as persons of the comparison group without rough somatic pathology. In control glioma C6 cultures in standard conditions cell clusters were formed by the type of "spheroids", from which radial cell migration occurred, a tense cellular or reticular growth zone was formed, and tumour cells preserved their ability to mitotic division. Under the influence of platelet-released secretion products of patients with glioma, differently directed effects on cell mitotic activity and the number of cell clusters in glioma C6 cultures were detected depending on the degree of tumour malignancy: stimulating effect under the influence of platelet factors of patients with high-malignancy glioma (G4) and inhibitory effect – due to the influence of platelet factors of patients with differentiated glioma (G2). In contrast to the thrombocyte-released factors, the conditioned media of a common pool of peripheral blood cells of patients with G4 glioma suppressed the mitotic activity of tumour cells and did not affect the number of cell clusters. No changes in glioma C6 cultures were revealed after the influence of platelet-released secretion products of persons of the comparison group. The obtained data confirm the important role of platelets in the pathogenesis of brain glioma, pointing to the fundamental difference in the spectrum of biologically active molecules that are released by platelets of patients depending on the degree of tumour malignancy and are able to regulate the cell cycle and proliferative activity of the glioma tumour cells, which may have application as a diagnostic marker as well as predictive marker of response to antitumour therapy.

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Receptor Mediated Binding of the Fibrinolytic Components, Plasminogen and Urokinase, to Peripheral Blood Cells

Thrombosis and Haemostasis ◽

10.1055/s-0038-1646019 ◽

1987 ◽

Vol 58 (03) ◽

pp. 936-942 ◽

Cited By ~ 96

Author(s):

Lindsey A Miles ◽

Edward F Plow

Keyword(s):

T Cells ◽

B Cell ◽

Peripheral Blood ◽

Binding Sites ◽

Blood Cells ◽

High Capacity ◽

Red Cells ◽

Peripheral Blood Cells ◽

Cellular Functions ◽

Fibrinolytic Proteins

SummaryGlu-plasminogen binds to platelets; the monocytoid line, U937, and the human fetal fibroblast line, GM1380 bind both plasminogen and its activator, urokinase. This study assesses the interaction of these fibrinolytic proteins with circulating human blood cells. Plasminogen bound minimally to red cells but bound saturably and reversibly to monocytes, granulocytes and lymphocytes with apparent Kd values of 0.9-1.4 μM. The interactions were of high capacity with 1.6 to 49 × 105 sites/cell and involved the lysine binding sites of plasminogen. Both T cells and non-rosetting lymphocytes and two B cell lines saturably bound plasminogen. Urokinase bound saturably to gianulocytes, monocytes, non-rosetting lymphocytes and a B cell line, but minimally to T cells, platelets and red cells. Therefore, plasminogen binding sites of high capacity, of similar affinities, and with common recognition specificities are expressed by many peripheral blood cells. Urokinase receptors are also widely distributed, but less so than plasminogen binding sites. The binding ol plasminogen and/ or urokinase to these cells may lead to generation of cell- associated proteolytic activity which contributes to a variety of cellular functions.

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Expression profiling of peripheral blood cells in patients with different subtypes of spinocerebellar ataxias

Aktuelle Neurologie ◽

10.1055/s-2005-919553 ◽

2005 ◽

Vol 32 (S 4) ◽

Author(s):

M Walter ◽

H Stappert ◽

L Schöls ◽

O Riess ◽

M Bonin

Keyword(s):

Peripheral Blood ◽

Expression Profiling ◽

Blood Cells ◽

Peripheral Blood Cells ◽

Spinocerebellar Ataxias

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GW26-e0264 Microarray analysis of differential gene expression profile in the peripheral blood cells of the patients with myocardial infarction

Journal of the American College of Cardiology ◽

10.1016/j.jacc.2015.06.596 ◽

2015 ◽

Vol 66 (16) ◽

pp. C155-C156

Author(s):

Xudong Guo ◽

Lin Fan ◽

Xiangdong Li ◽

Fanbo Meng

Keyword(s):

Gene Expression ◽

Myocardial Infarction ◽

Gene Expression Profile ◽

Differential Gene Expression ◽

Microarray Analysis ◽

Expression Profile ◽

Peripheral Blood ◽

Blood Cells ◽

Peripheral Blood Cells ◽

Differential Gene

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RNA Sequencing of Human Peripheral Blood Cells Indicates Upregulation of Immune-Related Genes in Huntington's Disease

Frontiers in Neurology ◽

10.3389/fneur.2020.573560 ◽

2020 ◽

Vol 11 ◽

Author(s):

Miguel A. Andrade-Navarro ◽

Katja Mühlenberg ◽

Eike J. Spruth ◽

Nancy Mah ◽

Adrián González-López ◽

...

Keyword(s):

Gene Expression ◽

Huntington's Disease ◽

Huntington’S Disease ◽

Peripheral Blood ◽

Blood Cells ◽

Trinucleotide Repeat ◽

Neurodegenerative Disorder ◽

Gene Expression Signature ◽

Interferon Response ◽

Peripheral Blood Cells

Huntington's disease (HD) is an autosomal dominantly inherited neurodegenerative disorder caused by a trinucleotide repeat expansion in the Huntingtin gene. As disease-modifying therapies for HD are being developed, peripheral blood cells may be used to indicate disease progression and to monitor treatment response. In order to investigate whether gene expression changes can be found in the blood of individuals with HD that distinguish them from healthy controls, we performed transcriptome analysis by next-generation sequencing (RNA-seq). We detected a gene expression signature consistent with dysregulation of immune-related functions and inflammatory response in peripheral blood from HD cases vs. controls, including induction of the interferon response genes, IFITM3, IFI6 and IRF7. Our results suggest that it is possible to detect gene expression changes in blood samples from individuals with HD, which may reflect the immune pathology associated with the disease.

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