scholarly journals Isolation and Characterization of Chitin Nanofibers from Calocybe indica and its Applications

2021 ◽  
Vol 11 (4) ◽  
pp. 4105-4113
Keyword(s):  
Antioxidant Activity ◽  
Hydroxyl Radical ◽  
Reduced Glutathione ◽  
Analytical Techniques ◽  
Scavenging Activity ◽  
Isolation And Characterization ◽  
Chemically Treated ◽  
Calocybe Indica ◽  
Chitin Nanofibers

In this study, chitin nanofibers are isolated from Calocybe indica (Milky White Mushroom), and they are further characterized using various analytical techniques such as UV-VIS, FTIR, TGA, Fe - SEM, and XRD. Antioxidant activity of the chitin nanofibers was studied by performing various assays such as DPPH, ABTS, H2O5 Scavenging Activity, Hydroxyl Radical Activity, Superoxide Assay, and Reduced Glutathione Activity. Isolated Chitin nanofibers were then chemically treated to form hydrogels. The hydrogels formed were further characterized.

Deinbollia pinnata: Isolation and Characterization of Some Phytochemicals from Aerial Parts and their Antioxidant Potential

2020 ◽  
Vol 10 ◽  
Author(s):  
Yakubu Rufai ◽  
Norazah Basar ◽  
Aliyu Sani
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Root Bark ◽  
Ferric Ion ◽  
Scavenging Activity ◽  
Methanol Extracts ◽  
Isolation And Characterization ◽  
Ic50 Value

Background: Deinbollia pinnata belonging to Sapindaceae family are known as medicinal plants for the treatment of human diseases. Because of human over-exploitation, the choice of phytochemicals re-search and screening was basically made to add to the available compounds and ethno-medicinal records. Objective: To investigate phytochemicals and their antioxidant activities. Method: Antioxidant assays (DPPH, ABTS, FRAP) activities; phytochemical contents (Folin Ciocalteaus’s (FC) and aluminum chloride) methods; and phytochemical analysis were employed. Successful characterization of isolates using spectroscopic methods (1H NMR, 13C NMR, FTIR and GC-MS analysis). Results: Alkaloid, simple sugar, phenolics, tannins and steriods were present in all the parts. Saponin, terpenes and flavonoids were found in both leaves and root bark, while root bark and heartwood possessed quinones. The methanol extracts demonstrated total phenolic content at 75. 9 ± 2.05 GAE (mg GAE/g dw) and total flavonoid content at 88.41 ± 0.56 QUE (mg QUE/g dw). The plant extracts showed good DPPH scavenging activity with percentage inhibition more than 70% at 125 ppm. The methanol extracts showed excellent antioxidant activity towards ABTS assay with the IC50 value of 13.68 µg/mL. The extracts also exhibited the most potent ferric ion reducer at 3.45 ± 1.30 mM FRAP equivalent. Methyl gallate (2) displayed the highest antioxidant radical scavenging activity towards DPPH assay with the IC50 value of 0.19 µg/mL and pyrogallol (4) showed the highest ABTS activity with the SC50 value of 7.44 µg/mL. While, 3,4-dihydroxybenzoic acid (1) also showed the potential as ferric ion reducer ranging from 0.67± 0.01 to 3.63±0.12 mM FRAP equivalent respectively. Conclusion: Based on the antioxidant activity, methanolic extracts and its isolated constituents can be used as antioxidant sources for the management of oxidative causative diseases. Being the first report of these phytochemicals from genius Deinbollia and specie pinnata.

scholarly journals Biflavonoids from the Unripe Fruits of Clusia Paralicola and their Antioxidant Activity

2012 ◽  
Vol 7 (12) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Rafaela Ferreira Oliveira ◽  
Celso Amorim Camara ◽  
Maria de Fátima Agra ◽  
Tania Maria Sarmento Silva
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Spectral Data ◽  
2D Nmr ◽  
Cd Spectra ◽  
Total Extract ◽  
Isolation And Characterization ◽  
Β Carotene ◽  
Unripe Fruits

Investigation of the green fruits of Clusia paralicola (Clusiaceae) led to the isolation and characterization of two 3,8″-biflavonoids, 2R, 3S, 2″R, 3″R-GB1-7″- O-β-glucoside (1) and 2R, 3S, 2″R, 3,8″-binaringenin-7″-O-β-glucoside (2), together with four known compounds: β-sitosterol, stigmasterol, β-amyrin, and epicatechin. The structures were established from the IR, LC-ESI-MS and NMR spectral data, including 2D-NMR experiments. The absolute configurations of 1 and 2 were determined by CD spectra. The total extract and the biflavonoids demonstrated significant antioxidant activity in DPPH, ABTS, and β-carotene/linoleic acid tests.

scholarly journals Antioxidant Activity of Hypericum androsaemum Infusion: Scavenging Activity against Superoxide Radical, Hydroxyl Radical and Hypochlorous Acid

2002 ◽  
Vol 25 (10) ◽  
pp. 1320-1323 ◽  
Author(s):  
Patrícia Valentão ◽  
Eduarda Fernandes ◽  
Félix Carvalho ◽  
Paula Branquinho Andrade ◽  
Rosa Maria Seabra ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Hydroxyl Radical ◽  
Superoxide Radical ◽  
Hypochlorous Acid ◽  
Scavenging Activity

Preparation and characterization of phloretin by complexation with cyclodextrins

2020 ◽  
Vol 44 (14) ◽  
pp. 5218-5223
Author(s):  
Xiaona Hu ◽  
Ziruo Zhou ◽  
Lijun Han ◽  
Shujing Li ◽  
Wei Zhou
Keyword(s):  
Antioxidant Activity ◽  
Inclusion Complexes ◽  
Scavenging Activity ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

RP and DPPH scavenging activity tests revealed that the antioxidant activity of phloretin improved after the formation of inclusion complexes.

Study on Antioxidant Activity of Dihydromyricetin-Zinc(II) Complex

2011 ◽  
Vol 183-185 ◽  
pp. 863-867 ◽  
Author(s):  
Chun Wu ◽  
Xin Ping Zheng ◽  
Lin Lin Chen
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Hydroxyl Radical ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Free Radical Scavenger ◽  
Superoxide Anion Radical ◽  
Radical Scavenger ◽  
Scavenging Activity ◽  
Dpph Radical

Dihydromyricetin-zinc(II) complex was synthesized. The scavenging activity of DPPH radical, hydroxyl radical, superoxide anion radical and alkane radical by dihydromyricetin-zinc(II) complex were determine, moreover compared with Vc. The results showed that dihydromyricetin-zinc(II) complex possessed strong scavenging activities on DPPH•, hydroxyl radical, superoxide anion radical and alkane radical with correspounding maximum scavenging rate 74%, 70% , 65% and 50% at the concentration from 0.1 mmol/L to 0.5 mmol/L, respectively, which were higher than Vc. These results suggested that dihydromyricetin-zinc(II) complex would be an effective free radical scavenger.

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