Deinbollia pinnata: Isolation and Characterization of Some Phytochemicals from Aerial Parts and their Antioxidant Potential

2020 ◽  
Vol 10 ◽  
Author(s):  
Yakubu Rufai ◽  
Norazah Basar ◽  
Aliyu Sani
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Root Bark ◽  
Ferric Ion ◽  
Scavenging Activity ◽  
Methanol Extracts ◽  
Isolation And Characterization ◽  
Ic50 Value

Background: Deinbollia pinnata belonging to Sapindaceae family are known as medicinal plants for the treatment of human diseases. Because of human over-exploitation, the choice of phytochemicals re-search and screening was basically made to add to the available compounds and ethno-medicinal records. Objective: To investigate phytochemicals and their antioxidant activities. Method: Antioxidant assays (DPPH, ABTS, FRAP) activities; phytochemical contents (Folin Ciocalteaus’s (FC) and aluminum chloride) methods; and phytochemical analysis were employed. Successful characterization of isolates using spectroscopic methods (1H NMR, 13C NMR, FTIR and GC-MS analysis). Results: Alkaloid, simple sugar, phenolics, tannins and steriods were present in all the parts. Saponin, terpenes and flavonoids were found in both leaves and root bark, while root bark and heartwood possessed quinones. The methanol extracts demonstrated total phenolic content at 75. 9 ± 2.05 GAE (mg GAE/g dw) and total flavonoid content at 88.41 ± 0.56 QUE (mg QUE/g dw). The plant extracts showed good DPPH scavenging activity with percentage inhibition more than 70% at 125 ppm. The methanol extracts showed excellent antioxidant activity towards ABTS assay with the IC50 value of 13.68 µg/mL. The extracts also exhibited the most potent ferric ion reducer at 3.45 ± 1.30 mM FRAP equivalent. Methyl gallate (2) displayed the highest antioxidant radical scavenging activity towards DPPH assay with the IC50 value of 0.19 µg/mL and pyrogallol (4) showed the highest ABTS activity with the SC50 value of 7.44 µg/mL. While, 3,4-dihydroxybenzoic acid (1) also showed the potential as ferric ion reducer ranging from 0.67± 0.01 to 3.63±0.12 mM FRAP equivalent respectively. Conclusion: Based on the antioxidant activity, methanolic extracts and its isolated constituents can be used as antioxidant sources for the management of oxidative causative diseases. Being the first report of these phytochemicals from genius Deinbollia and specie pinnata.

scholarly journals ANTIOXIDANT ACTIVITY, PHYTOCHEMICAL ANALYSIS AND TOTAL POLYPHENOLICS CONTENT OF ESSENTIAL OIL, METHANOL EXTRACT AND METHANOL FRACTIONS FROM COMMELINA NUDIFLORA

2018 ◽  
Vol 10 (8) ◽  
pp. 36 ◽  
Author(s):  
Muhammad Dawood Shah ◽  
Mohammad Iqbal
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Ethyl Acetate ◽  
Palmitic Acid ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Hexadecanoic Acid ◽  
Methanol Extracts

Objective: In the present study, the essential oil, methanol extract, and methanol fractions (n-hexane, chloroform, ethyl acetate, and n-butanol) obtained from Commelina nudiflora were investigated for the free radical scavenging effects and phytochemical analysis.Methods: The antioxidative effect of the essential oil, methanol extracts and methanol fractions were evaluated using 2, 2 diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Total phenolic and flavonoid contents were determined using Folin-Ciocalteau and aluminium chloride reagents respectively. The phytochemical analyses of the essential oil, methanol extracts and methanol fractions were performed by gas chromatography and mass spectrometry (GCMS). Results: The antioxidant, total phenolic and total flavonoid contents of butanol, ethyl acetate and chloroform fractions were higher followed by methanol extract, hexane fraction and essential oil. Phytochemical analysis indicated the presence of alkaloid, saponin, steroid, phytosterols, triterpenoids and tannins etc. The identified bioactive constituents of essential oil, methanol extract and methanol fractions of C. nudiflora were indole, 2-methoxy-4-vinylphenol, 2-pentadecanone, 6,10,14-trimethyl, phenol, benzyl alcohol, eugenol, phenol, 2, 4-bis (1,1-dimethylethyl), hexadecanoic acid, ethyl ester (palmitic acid ester), n-hexadecanoic acid (palmitic acid), 9, 12-octadecadienoic acid, (linoleic acid) and phytol. All identified bioactive compounds and their derivatives were generally reported with antimicrobial, antioxidant, anti-inflammatory and antitumor properties.Conclusion: The obtained data suggest that the essential oil, methanol extract and methanol fractions of C. nudiflora possess remarkable antioxidant activities and vital phytochemicals. Thus the plant can be a utilized as a potential source of nutraceutical with antioxidant activity.

scholarly journals In vitro assessment of the protection from oxidative stress by various fractions of Artemisia incisa Pamp.

2011 ◽  
Vol 76 (10) ◽  
pp. 1379-1386
Author(s):  
A Aziz-Ur-Rehman ◽  
Sehrish Gulzar ◽  
Muhammad Abbasi ◽  
Tayyaba Shahzadi ◽  
Tauheeda Riaz ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Butylated Hydroxytoluene ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Aqueous Fraction ◽  
Antioxidant Power ◽  
Ic50 Value

The methanolic extract of Artemisia incisa Pamp. was dissolved in distilled water and successively partitioned with n-hexane, chloroform, ethyl acetate and n-butanol. The antioxidant potential of all these fractions and remaining aqueous fraction was evaluated by four methods, i.e., the scavenging activity of the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), the total antioxidant activity, the ferric reducing antioxidant power (FRAP) assay and the ferric thiocyanate assay. In addition, the total phenolics was determined. The obtained results revealed that among the studied fractions the ethyl acetate soluble fraction showed the most potent DPPH-radical scavenging activity with an IC50 value of 5.3 ? 0.71 ?g mL-1, which is even more effective than the standard antioxidant butylated hydroxytoluene (BHT) (IC50 value 0f 12.1? 0.92 ?g mL-1). The ethyl acetate fraction also showed the highest FRAP value (3677.13 ? 27.1 ?g TE mL-1), inhibition of lipid peroxidation (60.93 ? 0.84 % at 500 ?g mL-1) and total phenolic content (95.5 ? 0.05 ?g GAE g-1) as compared to other fractions. However, the remaining aqueous fraction was found to posses the highest antioxidant activity of all the fractions.

Antioxidant profiling of Hippophae salicifolia growing in sacred forests of Sikkim, India

2011 ◽  
Vol 38 (9) ◽  
pp. 697 ◽  
Author(s):  
Arvind K. Goyal ◽  
Bharat C. Basistha ◽  
Arnab Sen ◽  
Sushil K. Middha
Keyword(s):  
Antioxidant Activity ◽  
Sea Buckthorn ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Methanol Extracts ◽  
Hippophae Salicifolia ◽  
Total Antioxidant ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Phenolic And Flavonoid

Hippophae commonly known as sea-buckthorn, is a versatile plant with multipurpose uses including the control of soil erosion, biological nitrogen fixation and medicinal properties. In India, two species of sea-buckthorn; namely, Hippophae salicifolia D. Don and Hippophae rhamnoides L., are very common. H. salicifolia has a shrub-to-tree habit and is restricted to the Himalayan region, whereas H. rhamnoides is bushy, growing at higher altitude in India, and widely distributed in Europe and Asia. In this study, aqueous, methanol and acetone extracts of various parts of female and male plants of H. salicifolia were evaluated for total antioxidant activity, phenolic and flavonoid contents, and correlations were made. The total phenolic and flavonoid content in all the samples ranged from 99 ± 32 to 1459 ± 53 mg gallic acid equivalent g–1, 135 ± 12 to 707 ± 62 mg quercetin equivalent g–1 of dry extract, respectively. The highest free radical (DPPH) scavenging activity was found in methanol extracts of female bark and male leaf. Methanol extracts of male bark and male leaf showed maximum H2O2 activity at a concentration of 1 mg mL–1. Antioxidant activity (FRAP method) ranged from 0.021 to 1.041 at concentrations ranging from 20 to 200 µg mL–1 with a reducing capacity of extracts as water > methanol > acetone. Strong correlation (P < 0.05) was observed between DPPH scavenging activity and total phenolic content from all the extracts under study.

scholarly journals In vitro antioxidant activity and qualitative phytochemical analysis of two Vismia (Hypericaceae) species collected in Los Andes, Venezuela

2016 ◽  
Vol 64 (4) ◽  
Author(s):  
Alexis Buitrago ◽  
Janne Del Carmen Rojas ◽  
Yonel Peñalosa
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Methanol Extracts ◽  
Phenol Derivatives ◽  
Methanolic Extracts ◽  
High Concentrations

Vismia genus is distributed mainly in tropical and subtropical regions of Central, South America and some areas of Africa. According to previous investigations, antioxidant potential of Vismia species might be related to anthrones, anthraquinones, flavonoids and phenol derivatives biosynthesized by these plants. The aim of present study is to evaluate the free radical scavenging capacity, total phenolic and flavonoids content as well as the qualitative phytochemical screening of methanol extracts obtained from Vismia macrophylla (VM) and Vismia baccifera (VB) collected in Táchira and Mérida state, Venezuela. Phytochemical screening of VB and VM methanolic extracts carried out using various chemical assays revealed an abundant presence of anthraquinones in both species analyzed. Glycosides were also present while flavones and dehydroflavones were observed abundantly in VB but moderated in VM. Triterpenes were also detected and steroids showed to be abundant in VM but moderate in VB. On the other hand, antioxidant capacity measured by the DPPH radical scavenging assay showed that VM possesses a stronger antioxidant activity than VB with IC50 5.50 µg/mL. Phenol and flavonoid assays also revealed that methanol extracts of both VM and VB contain high concentrations of these metabolites. A relationship between the antioxidant activity, total phenol and flavonoids content of the extracts analyzed was demonstrated in present investigation since those samples with higher phenolic concentrations showed likewise higher antioxidant activity.

scholarly journals PHYTOCHEMICALS AND ANTIOXIDANT ACTIVITY OF CLERODENDRUM PANICULATUM (L.) LEAF AND FLOWER EXTRACTS

2021 ◽  
pp. 31-34
Author(s):  
DEEPA R HEBBAR ◽  
DEVIKA M ◽  
RASHMI GN ◽  
NALINI MS
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Leaf Extracts ◽  
Hexane Extracts ◽  
Reducing Power Assay

Objective: This study was designed to evaluate the phytochemicals present in the flower and leaf extracts of Clerodendrum paniculatum L., collected from Nelji village of Kodagu district . Methods: The healthy leaves and flowers of C. paniculatum were collected and the plant extracts were prepared using ethanol, hexane and distilled water separately. Phytochemical analysis was conducted using standard procedurs for the flower and leaf extracts of C. paniculatum. The antioxidant activity in leaf and flower extracts was determined by three assays, estimation of total phenolic content, reducing power assay and radical scavenging activity (DPPH) using standard procedures. Results: Phytochemical screening conducted for the flower and leaf extracts of C. paniculatum showed the presence of three phytochemicals, namely saponins, alkaloids and terpenoids. Terpenoids were commonly present in all the extracts of flower and leaf that is in both polar (aqueous and ethanol) solvent and in non-polar (hexane) solvents. The extracts tested for the antioxidant activity showed the presence of total phenolics in ethanol, aqueous and hexane extracts. The aqueous extract showed high redox potential followed by ethanol and hexane extracts. The aqueous leaf extract showed high radical scavenging activity when compared to the flower extracts of C. paniculatum. Conclusion:  The present study showes C. paniculatum to be an important medicinal plant, since the flower and leaves showed good antioxidant activity. Thus it may used in the treatment of diseases and may also used in the preparation of natural or herbal drugs due to the presence of antioxidants.

scholarly journals Evaluation of antioxidant activity of unpurified and purified datura seed

2021 ◽  
Vol 7 (1) ◽  
pp. 030-035
Author(s):  
Umang Haribhai Gajjar ◽  
Niralee Kalpeshkumar Velhal ◽  
Hetvi Girdharbhai Parikh ◽  
Jayrajsinh Bhartbhai Parmar ◽  
Yash Bhut ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Reducing Power ◽  
Datura Stramonium ◽  
Cow Milk ◽  
Ferric Ion ◽  
Scavenging Activity ◽  
Ic50 Value ◽  
Cow Urine ◽  
Assay Result

Aim: Evaluation of effect of shodhana process of datura seed (Datura stramonium) antioxidant activity. Methods: The datura was purified with two different processes viz. cow’s urine, and cow’s milk by Dolayantra method. Antioxidant activity was evaluated by in-vitro 1,1-Diphenyl-2-picryl hydrazyl (DPPH) radicals scavenging activity and Ferric reducing power ability (FRPA) assay. Result: cow urine purified datura (CUD) showed a significant effect in inhibiting DPPH, reaching up to 85.96% at concentration 1000 mcg/ml and its IC50 was 314.3 mcg/ml while Unpurified Datura Seed (UD) reaching up to 86.06% at concentration 1000 mcg/ml and its IC50 was 171.73 mcg/ml. The Cow Milk Purified Datura Seed (CMD) extract reach 69.72% at concentration 1000 mcg/ml and its IC50 value was 640. The IC50 value of Ascorbic acid was 23.35 mcg/ml. CUD showed a significant effect in reduction of ferric ion, reaching up to 85.59% reduction at concentration 1000 mcg/ml and its EC50 was 304.46 mcg/ml while UD reaching up to 86.11% at concentration 1000 mcg/ml and its EC50 was 280.28 mcg/ml. CMD extract reach 52.28% at concentration 1000 mcg/ml and its EC50 value was 1091.3mcg/ml.

scholarly journals Isolation and Characterization of Chitin Nanofibers from Calocybe indica and its Applications

2021 ◽  
Vol 11 (4) ◽  
pp. 4105-4113
Keyword(s):  
Antioxidant Activity ◽  
Hydroxyl Radical ◽  
Reduced Glutathione ◽  
Analytical Techniques ◽  
Scavenging Activity ◽  
Isolation And Characterization ◽  
Chemically Treated ◽  
Calocybe Indica ◽  
Chitin Nanofibers

In this study, chitin nanofibers are isolated from Calocybe indica (Milky White Mushroom), and they are further characterized using various analytical techniques such as UV-VIS, FTIR, TGA, Fe - SEM, and XRD. Antioxidant activity of the chitin nanofibers was studied by performing various assays such as DPPH, ABTS, H2O5 Scavenging Activity, Hydroxyl Radical Activity, Superoxide Assay, and Reduced Glutathione Activity. Isolated Chitin nanofibers were then chemically treated to form hydrogels. The hydrogels formed were further characterized.

scholarly journals Radical Scavenging Activity and Total Phenolic Content of Extracts of the Root Bark of Osyris Lanceolata

2009 ◽  
Vol 4 (1) ◽  
pp. 1934578X0900400
Author(s):  
Elizabeth M. O. Yeboah ◽  
Runner R. T. Majinda
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Root Bark ◽  
Fast Kinetics ◽  
Methanol Extracts ◽  
Slow Kinetics

The root bark of Osyris lanceolata was screened for its antioxidant potential using three variations of the DPPH radical scavenging method viz a TLC-autographic assay, a semi-quantitative TLC assay, and a spectrophotometric assay. The total phenolic content was evaluated as gallic acid equivalents (GAE) using the Folin-Ciocalteu method. The powdered root bark was extracted sequentially with n-hexane, chloroform, methanol and 90% methanol/water. A separate supercritical fluid extraction (SFE) of the root bark was also carried out. The radical scavenging ability, as measured by IC50 values, was found to be 48.4 ± 0.4 and 49.5 ± 1.9 μg/mL for the 90% methanol/water and methanol extracts respectively, while those of the chloroform, n-hexane, and SFE extracts were found to be < 250 μg/mL. Under the same conditions, the values for the standards, ascorbic acid and gallic acid, were found to be 38.70 ± 0.08 and 2.86 ± 0.01 μg/mL respectively. The radical scavenging power of the five extracts correlated positively with the total phenolic content and was ranked in the following decreasing order: 90% methanol/water > methanol > chloroform > SFE > n-hexane with total phenolic contents of 290.2 ± 3.3, 271.0 ± 3.7, 74.4 ± 0.8, 62.5 ± 0.4 and 49.5 ± 1.9 mg GAE/g of dry extract, respectively. The 90% methanol/water and methanol extracts showed several components with high antioxidant activity displaying fast kinetics in both TLC assays, while the chloroform, SFE, and n-hexane extracts exhibited slow kinetics antioxidant activity.

scholarly journals Pentacyclic Triterpenoids from Maytenus cuzcoina

2017 ◽  
Vol 12 (5) ◽  
pp. 1934578X1701200
Author(s):  
Carolina P. Reyes ◽  
Ignacio A. Jiménez ◽  
Isabel L. Bazzocchi
Keyword(s):  
2D Nmr ◽  
Bark Extract ◽  
Phytochemical Analysis ◽  
Spectroscopic Methods ◽  
Root Bark ◽  
Pentacyclic Triterpenoids ◽  
Isolation And Characterization ◽  
Nmr Techniques ◽  
First Time

Phytochemical analysis of the root bark extract of Maytenus cuzcoina (Celastraceae) led to the isolation and characterization of sixteen triterpenoids with a 6/6/6/6/6 pentacyclic system, including the new 22α-hydroxy-29-methoxy-3β-tetradecanoate-olean-12-ene, and 3β,24β-epoxy-29-methoxy-2α,3α,6α-trihydroxy- D:A-friedelane that is reported for the first time as a natural product. This is the first instance of the isolation of a tetradecanoate triterpene ester from a Celastraceae species. The structures were elucidated on the basis of spectrometric and spectroscopic methods, including 1D and 2D NMR techniques.

scholarly journals In vitro antioxidant activity of Bougainvillea glabra and Mucuna pruriens

2020 ◽  
Vol 11 (1) ◽  
pp. 806-812
Author(s):  
GopiKrishna Rakam ◽  
Raja Sundararajan
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Anion ◽  
Mucuna Pruriens ◽  
Scavenging Activity ◽  
Methanol Extracts ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Bougainvillea Glabra ◽  
In Vitro Antioxidant Activity

The purpose of the contemporary in vitro work implemented was to examine the antioxidant effect of Bougainvillea glabra and Mucuna pruriens methanol extracts. Methanol extracts of Bougainvillea glabra and Mucuna pruriens were screened individually for different in vitro antioxidant activity. Commercial antioxidants such as vitamin E, Ascorbic acid were used as reference standard. For ABTS+ assay IC50 value of Bougainvillea glabra & Mucuna pruriens metha­nol extract were found to be 17.1μg/ml and 23.2μg/ml correspondingly. Likewise, the IC50 values for xanthine oxidase inhibitory activity for Bougainvillea glabra and Mucuna pruriens was found to be 46.7μg/ml and 95.1μg/ml, respectively. For scavenging of various assay models like nitric oxide, hydroxyl and superoxide anion scavenging activity, the IC50 values for Bougainvillea glabra were found to be 45.3μg/ml, 70.1μg/ml and 38.1μg/ml respectively. The IC50 values for Mucuna pruriens for nitric oxide, hydroxyl and superoxide anion scavenging activity, were found to be 92.1μg/ml, 43.4μg/ml and 50.3μg/ml respectively. Bougainvillea glabra and Mucuna pruriens methanol extracts showed 50 % inhibition effect at concentrations 118 μg/ml and 121 μg/ml respectively for lipid peroxidation assay. The IC50 values for DPPH scavenging assay for Bougainvillea glabra and Mucuna pruriens was found to be 13.24μg/ml and 14.4μg/ml correspondingly. For DMPD assay Bougainvillea glabra and Mucuna pruriens possess the IC50 value 16.34 μg/ml and 17.4 μg/ml correspondingly. Methanol extracts of Bougainvillea glabra and Mucuna pruriens exhibited potent in vitro antioxidant activity.

Sign in / Sign up

Export Citation Format

Share Document