Biodegradation Of Iron Industry And Gold Industrial Wastewater By White Rot Fungi – Calocybe Indica & Agaricus Bisporus Comparative Study

Increasing discharge and improper management of liquid and solid industrial wastes have created a great concern among industrialists and the scientific community over their economic treatment and safe disposal. Hence, there is a growing need for the development of novel, efficient, eco-friendly, and cost-effective approach for the remediation for these industries released into the environment and to safeguard the ecosystem. In this regard, recent advances in wastewater of heavy metal have propelled bioremediation as a prospective alternative to conventional techniques. Heavy metals are toxic and dangerous to the ecosystem. White rot fungi (WRF) are versatile and robust organisms having enormous potential for oxidative bioremediation of a variety of toxic chemical pollutants due to high tolerance to toxic substances in the environment. The decolorization and detoxification potential of WRF can be harnessed thanks to emerging knowledge of the physiology of these organisms as well as of the bio catalysis and stability characteristics of their enzymes. This knowledge will need to be transformed into reliable and robust waste treatment processes.

Effect of Milky Mushroom (Calocybe indica) in Different Agricultural Residues with Different Casing Materials

Present study entitled was carried out in field irrigation lab, Department of Agricultural Engineering, Aditya Engineering College, Surampalem. In the present experiment locally available agricultural substrate paddy straw, sugarcane bagasse and cocopeat were tested with black soil and farm yard manner as casing materials. For parameters such as days required for spawn run, pinhead formation, harvest days, total yield and biological efficiency were determined. Significant results were obtained for all the experimental parameters and also for the stem length and cap diameter. Best substrate found was the paddy straw in the recent experiment with days required for spawn run (14 days), pin head formation (11.2 days), days for first harvest(50 days), stem length (8.07 cm), capdiameter (8.67 cm), first harvest (489.45 gm), second harvest (453.68 gm), total yield (925.24 gm), biological efficiency (94.31%). The next best in the order was coco peat followed by sugarcane bagasse. Hence this study confirms the suitability of paddy straw for cultivation to mushroom growers and farmyard manure with 15 cm casing thickness as substrate for its cultivation for achieving higher yield. The benefit cost ratio was estimated as 1.57.

Evaluation of Different Substrates for Spawn Preparation of Calocybe indica and its Impact on Yield and Biological Efficiency

Background: Popularities of mushrooms is because of attractive texture and delicious taste, among the cultivated mushrooms milky mushroom has ability to grow in higher temperature and is also considered to be alternative of non-veg foods for vegetarians. In India some part of the country holds good promise towards the cultivation of milky mushroom, for fast growth of mycelium or easy cultivation. This experiment is mainly focused on evaluation of yield and yield contributing characteristic of milky mushroom (Calocybe indica). Methods: The spawn production process was carried out at the Experiential Learning Unit, Mushroom Laboratory, Department of Plant Pathology, Institute of Agriculture, Palli Sikha Bhavana, Visva Bharati in the year 2018-2019. Pure culture of milky mushroom procured from Tropical Mushroom Research Centre, OUAT, Bhubaneswar and was maintained on PDA medium. Different food grains for preparation of spawn viz., wheat grain, paddy grain, bajra grain and sorghum grain were used. Result: In the present work, we have observed that the four easily available food grains can efficiently be used as substrate for preparation of spawn. This study enables us to take decision about selection of food grains for quality spawn production.

Isolation and Characterization of Chitin Nanofibers from Calocybe indica and its Applications

In this study, chitin nanofibers are isolated from Calocybe indica (Milky White Mushroom), and they are further characterized using various analytical techniques such as UV-VIS, FTIR, TGA, Fe - SEM, and XRD. Antioxidant activity of the chitin nanofibers was studied by performing various assays such as DPPH, ABTS, H2O5 Scavenging Activity, Hydroxyl Radical Activity, Superoxide Assay, and Reduced Glutathione Activity. Isolated Chitin nanofibers were then chemically treated to form hydrogels. The hydrogels formed were further characterized.

Calocybe indicia extract modifies GABA and Serotonin levels to Alleviate anxiety in experimental adult Zebrafish models

Zebrafish (Danio rerio) was used as a model to study anxiety due to its physiological homology to humans. The pathophysiology of anxiety, even though still unclear, has been extensively studied in Zebrafish. Anxiety was induced by withdrawal after exposure to 0.5% ethanol, which proved to be anxiogenic, validated through the novel tank test. The light/dark test revealed that exposure to 0.5% ethanol had anxiolytic effects. The milky mushroom, Calocybe indica was used to treat anxiety since its anti-hypertensive effects have already been reported. Biochemical parameters such as GABA and MAO (A&B) were measured before and after treatment with different concentrations of C. indica and standard anxiolytic drug, Fluoxetine to compare and confirm the anxiolytic effect. The GABA content was found to be 119.9±1.99 mmoles/g tissue weight after treatment with 50 µg C. indica which was comparable to the normal group values (100±4.12). MAO (A&B) activity decreased which in turn increased serotonin levels with 25µg of C. indica. 25µg and 100µg concentration of the extract of C. indica was found to be optimum in reducing the level of anxiety.

Chemical Composition and Nutritional Value of Paddy Straw Milky Mushroom (Calocybe indica)

The edible milky mushroom is the most widely cultivated mushroom in tropical and sub-tropical regions. Studies were conducted on the cultivation and nutritional analysis of this mushroom. The pure culture of Calocybe indica was carried out in the laboratory. The culture was maintained on potato dextrose agar slant and sub-cultured at a regular monthly interval to sustain their fruiting vigor. The above stock culture was used in studies. Wheat grain spawn of C. indica was prepared in glass bottles. The filled paddy grain spawn of C. indica was used at 6% level to the wet weight of the substrate and the beds were spawned. After harvest, the mushrooms were dried at 40°C under the hot air oven to make it powered for further analysis. The collected fresh mushrooms were dried in shade and coarse powder was analyzed for nutrients namely moisture, crude protein, lipid, ash, crude fiber, and minerals. It shows the dominant compounds of protein, fiber, and carbohydrate and the contents are 14.11%, 8.30% and 5.62% respectively. Lipid, ash, ether extract and pH contents are 4.06%, 7.04%, 3.15% and 5.4% respectively. The carbon and nitrogen contents are 33.60% and 3.56% respectively. In the estimation of saturated fatty acids and unsaturated fatty acids present in C. indica, totally, 17 fatty acids were recorded. In the estimation of essential and non-essential amino acids present in C. indica, totally, 15 amino acids were recorded. In the estimation of minerals composition present in C. indica, it was recorded that the species had a good resource of mineral composition. Totally, 6 macro minerals and 8 micro minerals were recorded. This investigation may provide that the paddy straw substrates can be recommended for the commercial cultivation of C. indica to obtain highly nutritious mushrooms.

GC-MS Identification of Anti-inflammatory and Anticancer Metabolites in Edible Milky White Mushroom (Calocybe indica) against Human Breast Cancer (MCF-7) Cells

Milky White Mushroom is a species of edible mushroom native to India. The Purpose of this study was to determine the anti-inflammatory and anticancer compounds from Calocybe indica. The ethanolic extract from Calocybe indica were prepared. GC-MS was performed and the major bioactive compounds such as polysaccharides, amino acids, sterols, phytol, and squalene were determined. Calocybe indica was analyzed for their antioxidant activity through In vitro assays such as Free radical activity on DPPH, Hydroxyl radical scavenging assay, Nitric Oxide scavenging assay, Superoxide scavenging assay. Anti-inflammatory effect was estimated by protein denaturation inhibition assay, Proteinase inhibition assay, Cyclooxygenase inhibition assay and Lipoxygenase inhibition assay. Anticancer activity of Calocybe indica extract showed maximum inhibition of 69.11% of growth of human breast cancer cell (MCF 7) at 100μg/ml exposure for about 72 hours. At the end of this study, it was indicated that ethanolic extract of Calocybe indica can be used as an anti- inflammatory and anticancer agent.

Biosynthesis and Characterization of Zinc Nano particles Using Calocybe Indica and Analysis of their antimicrobial Activity

Recently, the use of nanotechnology has been expanding very rapidly in diverse areas of research, such as consumer products, energy, materials, and medicine. This is especially true in nanomedicine, due to physicochemical properties, such as mechanical, chemical, magnetic, optical, and electrical properties, compared with bulk materials. The first goal of this study was to produce Zinc oxide nanoparticles (ZnONPs) using two different biological resources as reducing agents, Calocybe indica fruiting bodies. The second goal was to investigate the antimicrobial potential of the as-prepared ZnONPs in lung cancer cells. The final goal was to investigate the role of p53 in the cellular response elicited by ZnONPs. The synthesis and characterization of ZnONPs were assessed by various analytical techniques, including ultraviolet-visible (UV-vis) spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and transmission electron microscopy (TEM). The present findings could provide insight into the future development of green synthesis in ZnO nanoparticles, along with their antimicrobial activity.

Anti-Obesity Properties of Calocybe Indica in Zebra fishes with Short-Term High-Fat Diet Induction

Obesity, a disease involved with complex health problems, is indicated by increased BMI, triglyceride and cholesterol levels. Obese individuals are found to be highly susceptible to develop non-alcoholic fatty liver disease,cardiovascular diseases, and also type 2 diabetes mellitus. Synthetic drugs used for treating obesity have been found to be associated with side effects such as anxiety,sleeplessness,hypertension, and drug addiction. Research on natural productspossessing therapeutic biological activitieshasdiscoveredtheir potential to minimize or even completely eliminate such side effects. Medicinal properties ofCalocybe indica include antidiabetic, hypertensive, anticancer, anti-inflammatory, antibacterial, and hepatoprotective effects; however, its anti-obesity activity is obscure.In this study, the anti-obesity effects of Calocybe indicawere investigated using a diet-induced obese Zebrafish modeland compared with standard drug Atorvastatin.Results show that 200µg of C. indica was able to effectively bring down triglyceride levels (12.5± 0 mg/ml; normal control 12.7 ± 0.7 mg/ml), cholesterol (210± 15.9 mg; normal control =70.4± 0)and HMG COA Reductase levels (0.9± 0.03; normal = 1.2 ± 0.01). Excessive fat accumulation in the liver (steatohepatitis) reduced after treatment with C. indica to a greater extent than by treatment with standard drug Atorvastatin. 100 µg of C. indica was found to be optimum in decreasing the levels of the liver enzymes, AST (177.1±5.7 IU/L; normal control =177.7±43.02 IU/l), ALT (365.5±2.9 IU/L; normal control= 355.5±34.4 IU/l), and ALP (2.3±1.1μmoles of phenol liberated/mg of protein/min; normal control = 0.7±1.2 μmoles of phenol liberated/mg of protein/min).Whole-body Oil Red O staining of the zebrafishes showed that with increasing concentration of C. indica, the accumulation of triglycerides and lipids decreased.