Study on Antioxidant Activity of Dihydromyricetin-Zinc(II) Complex

2011 ◽  
Vol 183-185 ◽  
pp. 863-867 ◽  
Author(s):  
Chun Wu ◽  
Xin Ping Zheng ◽  
Lin Lin Chen
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Hydroxyl Radical ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Free Radical Scavenger ◽  
Superoxide Anion Radical ◽  
Radical Scavenger ◽  
Scavenging Activity ◽  
Dpph Radical

Dihydromyricetin-zinc(II) complex was synthesized. The scavenging activity of DPPH radical, hydroxyl radical, superoxide anion radical and alkane radical by dihydromyricetin-zinc(II) complex were determine, moreover compared with Vc. The results showed that dihydromyricetin-zinc(II) complex possessed strong scavenging activities on DPPH•, hydroxyl radical, superoxide anion radical and alkane radical with correspounding maximum scavenging rate 74%, 70% , 65% and 50% at the concentration from 0.1 mmol/L to 0.5 mmol/L, respectively, which were higher than Vc. These results suggested that dihydromyricetin-zinc(II) complex would be an effective free radical scavenger.

scholarly journals Physicochemical and Antioxidant Properties of Acid- and Pepsin-Soluble Collagens from the Scales of Miiuy Croaker (Miichthys Miiuy)

Marine Drugs ◽  
2018 ◽  
Vol 16 (10) ◽  
pp. 394 ◽  
Author(s):  
Long-Yan Li ◽  
Yu-Qin Zhao ◽  
Yu He ◽  
Chang-Feng Chi ◽  
Bin Wang
Keyword(s):  
Amino Acid ◽  
Hydroxyl Radical ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Antioxidant Properties ◽  
Superoxide Anion Radical ◽  
Type I ◽  
Dpph Radical ◽  
Soluble Collagen ◽  
Miichthys Miiuy

In this report, acid-soluble collagen (ASC-MC) and pepsin-soluble collagen (PSC-MC) were extracted from the scales of miiuy croaker (Miichthys miiuy) with yields of 0.64 ± 0.07% and 3.87 ± 0.15% of dry weight basis, respectively. ASC-MC and PSC-MC had glycine as the major amino acid with the contents of 341.8 ± 4.2 and 344.5 ± 3.2 residues/1000 residues, respectively. ASC-MC and PSC-MC had lower denaturation temperatures (32.2 °C and 29.0 °C for ASC-MC and PSC-MC, respectively) compared to mammalian collagen due to their low imino acid content (197.6 and 195.2 residues/1000 residues for ASC-MC and PSC-MC, respectively). ASC-MC and PSC-MC were mainly composed of type I collagen on the literatures and results of amino acid composition, SDS-PAGE pattern, ultraviolet (UV) and Fourier-transform infrared spectroscopy (FTIR) spectra. The maximum solubility of ASC-MC and PSC-MC was appeared at pH 1–3 and a sharp decrease in solubility was observed when the NaCl concentration was above 2%. Zeta potential studies indicated that ASC-MC and PSC-MC exhibited a net zero charge at pH 6.66 and 6.81, respectively. Furthermore, the scavenging capabilities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical, superoxide anion radical and 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical of ASC-MC and PSC-MC were positively correlated with their tested concentration ranged from 0 to 5 mg/mL and PSC-MC showed significantly higher activity than that of ASC-MC at most tested concentrations (p < 0.05). In addition, the scavenging capability of PSC-MC on hydroxyl radical and superoxide anion radical was higher than those of DPPH radical and ABTS radical, which suggested that ASC-SC and PSC-SC might be served as hydroxyl radical and superoxide anion radical scavenger in cosmeceutical products for protecting skins from photoaging and ultraviolet damage.

A Study of Antioxidant Activity about Seleno-Polysaccharide of Cordyceps militaris from Different Areas of China

2013 ◽  
Vol 807-809 ◽  
pp. 1984-1987 ◽  
Author(s):  
Qin Li ◽  
Chi Zhang
Keyword(s):  
Antioxidant Activity ◽  
Hydroxyl Radical ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Reducing Power ◽  
Cordyceps Militaris ◽  
Superoxide Anion Radical ◽  
Scavenging Capacity ◽  
Research Objects ◽  
Total Selenium

In this article,Cordyceps militaris,that from Hubei and Yunnan,were used as the research objects, to extract seleno-polysaccharide ,then the antioxidant activity of seleno-polysaccharide were determined by reducing power,the scavenging capacity on superoxide anion radical and hydroxyl radical.The results indicated that,the content of selenium in Hubei and Yunnan Cordyceps militaris samples were 210.0μg/g and 57.5μg/g,respectively;the share of Se-polysaccharide in total selenium were 11.086% and 26.713%,respectively,lower degree to turn the selenium organic in Hubei Cordyceps militaris.The reducing power of seleno-polysaccharide from Hubei and Yunnan Cordyceps militaris were 0.0253,0.0297,respectively;two kinds of Cordyceps militaris have certain ability to remove superoxide anion radical and different between them,the seleno-polysaccharide of Yunnan Cordyceps militaris has a better effect to remove superoxide anion radical under different sample amount.About hydroxyl radical,there were no ability to remove it.

Activated oxygen species do not mediate hypercapnia-induced cerebral vasodilation in newborn pigs

1991 ◽  
Vol 261 (2) ◽  
pp. H335-H342 ◽  
Author(s):  
C. W. Leffler ◽  
R. Mirro ◽  
C. Thompson ◽  
M. Shibata ◽  
W. M. Armstead ◽  
...  
Keyword(s):  
Hydroxyl Radical ◽  
Xanthine Oxidase ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Superoxide Anion Radical ◽  
Radical Scavenger ◽  
Oxygen Species ◽  
Pial Arteries ◽  
Activated Oxygen ◽  
Newborn Pigs

In newborn pigs, vasodilation in response to hypercapnia is dependent on prostaglandin (PG) H synthase. We investigated the contribution of activated oxygen by-products to hypercapnia-induced PGH synthase-dependent dilation of pial arteries and arterioles in anesthetized newborn pigs. Activated oxygen species were generated on the cerebral surface using xanthine oxidase and hypoxanthine. Catalase, H2O2, and iron or N-(2-mercaptopropionyl)-glycine (MPG) were used to separate effects of superoxide anion and hydroxyl radical. All the activated oxygen species tested caused vasodilation of both arteries and arterioles. Vasodilation to all activated oxygen species was largely reversible with only the hydroxyl radical encouraging combination of xanthine oxidase, hypoxanthine, H2O2, and FeCl3, causing significant dilation 20 min after removal of treatment. Cotreatment with MPG blocked this residual dilation. Neither pretreatment with the extracellular superoxide anion radical scavenger, superoxide dismutase (SOD), the intracellular superoxide anion radical scavenger, Tiron, the H2O2 scavenger, catalase, nor hydroxyl radical scavengers, dimethyl sulfoxide (DMSO) and MPG, altered vasodilation of pial arteries or arterioles in response to hypercapnia. Furthermore, the increase in cerebral prostanoid synthesis in response to hypercapnia was not affected by pretreatment with SOD, Tiron, catalase, DMSO, or MPG. We conclude that the progressively reduced forms of oxygen that would be produced during PGH synthase metabolism of arachidonic acid can dilate pial arteries and arterioles of newborn pigs. However, these activated oxygen species are not responsible for the vasodilation to hypercapnia in the newborn pig, suggesting that eicosanoids cause the dilation.

Note: Antioxidant Properties of Lees Produced during Sake Making Using Fugu Muscle and Fin

2003 ◽  
Vol 9 (2) ◽  
pp. 123-126 ◽  
Author(s):  
T. Nagai
Keyword(s):  
Hydroxyl Radical ◽  
Food Processing ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Superoxide Anion Radical ◽  
Industrial Wastes ◽  
Dpph Radical ◽  
Enzymatic Hydrolysates

Enzymatic hydrolysates were prepared from lees produced during sake making using fugu muscle and fin. The antioxidant properties of these hydrolysates were measured using four different methods: autoxidation test, superoxide anion radical, DPPH radical and hydroxyl radical scavenging test. The yields of enzymatic hydrolysates were around 8.0% and their protein average content 0.66 mg/mg. The lees had remarkable antioxidant properties and high superoxide anion radical and hydroxyl radical scavenging abilities in comparison with those of ascorbic acid and α-tocopherol. According to the former results, lees produced during sake making could be used as a protein source with antioxidant properties for different applications in food processing, and its use could contribute to reduce industrial wastes.

Researches on the Structure and Antioxidant Activity of Apigenin-La (III) Complex

2013 ◽  
Vol 781-784 ◽  
pp. 1138-1143
Author(s):  
Feng Qiu Wen ◽  
Lang Sheng Pan ◽  
Lei Lei
Keyword(s):  
Thermal Analysis ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Superoxide Anion Radical ◽  
Scavenging Activity ◽  
Scavenging Effect ◽  
Pyrogallic Acid ◽  
Better Than

La (III) complex with apigenin was synthesized and characterized by UV, IR, 1HNMR and thermal analysis. The scavenging activity of the complex on superoxide anion radical (O2-·) and hdroxyl radical (·OH) was also investigated by improved pyrogallic acid way and ortho-oxybenzoic acid way. The results show that the complex had remarkable scavenging effects on O2-· and ·OH, and its scavenging effect on free radicals was better than apigenin.

Anti-Oxidant Activity of Anthocyanins of Black Soybean In Vitro

2013 ◽  
Vol 634-638 ◽  
pp. 1423-1428 ◽  
Author(s):  
Yan Song ◽  
Guo Ping Yu ◽  
Chao Ran Dou ◽  
Hao Wu ◽  
Zhu Gong
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Superoxide Anion Radical ◽  
Scavenging Activity ◽  
Antioxidant Power ◽  
Black Soybean

The aim of the work was to research antioxidant activity of the anthoyanins extracts of black soybean. The whole soybean through defatted, were to determine the phenolic and anthocyanin contents in black soybean extracts and to examine antioxidant activity of extract against DPPH and ABTS assays, and Ferric Reducing Antioxidant Power (FRAP), and superoxide anion radical scavenging activity. The defatted sample was extracted using ethanol by ultrasonic. The optimum DPPH and ABTS radical scavenging capacity (%) were 68.34% and 70.12%, VC was 59.76% and 95.23%, respectively, and contains high levels of reducing power and superoxide anion radical scavenging activity. The present results indicate that the anthoyanins extract from black soybean has greater anti-oxidative and is useful in functional food and other applications.

scholarly journals BIOMEDICAL POTENTIAL OF SELECTED MUSHROOM SPECIES

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Tatjana Stanojković ◽  
Marijana Kosanić
Keyword(s):  
Free Radical ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Superoxide Anion Radical ◽  
Total Phenol Content ◽  
Scavenging Activity

The aim of this study was to determine neuroprotective, antioxidant, antimicrobial and cytotoxic potential of acetone extracts of Coprinus comatus and Coprinopsis picacea mushrooms. The neuroprotetive activity was tested against acetylcholinesterase enzyme using the Ellman method. Antioxidant activity was evaluated by free radical scavenging ability, superoxide anion radical scavenging activity and reducing power. The total phenol content was examined using Folin-Ciocalteu reagent. The antimicrobial potential was determined by a microdilution method against 12 microorganisms. The cytotoxic activity was tested using MTT method on the Hela, A549 and LS174 cells. Our results indicate that C. comatus expressed a stronger neuroprotective effect (the percentage of inhibition of acetylcholinesterase was within the range 19.66-51.73%) than C. picacea. In antioxidant effect C. comatus had more potent free radical scavenging activity (IC50 = 276.69 μg/mL) and superoxide anion radical scavenging activity (IC50 = 39.40 μg/mL), while reducing power was relatively similar for both species. The total amount of phenols for C. comatus and C. picacea was 50.57 and 50.20 μg PE/mg of dry extracts, respectively. In antimicrobial activity, C. picacea showed a better effect with MIC values from 0.1 to 7.5 mg/mL. Finally, C. picacea expressed stronger cytotoxicity toward A549 and LS174 cells, while C. comatus was more active against Hela cell.

scholarly journals PEAT HUMIC ACIDS – PROSPECTIVE BIOLOGICALLY ACTIVE SUBSTANCES WITH ANTIOXIDANT ACTIVITY FOR THE DEVELOPMENT OF PROTECTIVE AGENTS

2021 ◽  
pp. 287-298
Author(s):  
Kristina Aleksandrovna Bratishko ◽  
Mariya Vladimirovna Zykova ◽  
Vladimir Vladimirovich Ivanov ◽  
Evgeniy Evgen'yevich Buyko ◽  
Larisa Aleksandrovna Drygunova ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Hydroxyl Radical ◽  
Sodium Hydroxide ◽  
Radical Cation ◽  
Humic Acids ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Water Soluble ◽  
Superoxide Anion Radical ◽  
Sodium Pyrophosphate

A present study was carried out to investigate the antioxidant activity (AOA) of humic acids (HA) isolated by sodium hydroxide and sodium pyrophosphate from nine peat species of the Tomsk region different by botanical composition, degree of decomposition and ash content. All HA samples had shown the AOA in the study. As the results of four research methods, it was found that HAs are highly effective in inhibiting the free ABTS•+ radical cation, superoxide anion radical O2-•, hydroxyl radical HO•, and are able to bind Fe2+ in a wide range of concentrations. The activity of HAs in different tests was not equal between samples; HAs isolated by sodium hydroxide have had a higher AOA in inhibition of the superoxide anion–radical O2-• in the model reaction compared to HAs isolated by sodium pyrophosphate within the same peat species. In the study of iron binding activity, an inverse relationship was observed. In the model reactions of the free ABTS•+ radical cation and the hydroxyl radical HO• inhibition, a comparable level of activity was registered between the HA samples isolated by different solutions. Such an uneven distribution of the activity between various HA samples can be explained by the unequal chemical parameters of their structure, which depend on their origin and method of isolation. Using of such classical antioxidants with an established mechanism of action as comparison drugs, as a water-soluble analogue of tocopherol - "Trolox", a water-soluble antioxidant – ascorbic acid, a classic chelator – ethylenediaminetetraacetic acid (EDTA), a classic trap of a hydroxyl radical – mannitol, it is possible to conclude that the investigated HAs are effective antioxidants belonging to the groups of proton donors and complexing agents.

Effect of Ultrasonic Degradation on In Vitro Antioxidant Activity of Polysaccharides from Porphyra yezoensis (Rhodophyta)

2008 ◽  
Vol 14 (6) ◽  
pp. 479-486 ◽  
Author(s):  
C. Zhou ◽  
Y. Wang ◽  
H. Ma ◽  
R. He
Keyword(s):  
Molecular Weight ◽  
Antioxidant Activity ◽  
Hydroxyl Radical ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Porphyra Yezoensis ◽  
Reducing Power ◽  
Superoxide Anion Radical ◽  
Sulfate Group ◽  
Ultrasonic Degradation

This article aims to study the effect of ultrasonic degradation on antioxidant activity of polysaccharides from Porphyra yezoensis (Rhodophyta) (PSPY), and to look for the relationship between molecular structure of fractions (F1, F2, and F3) from ultrasonic-degraded PSPY (UDPSPY) and their activities of scavenging hydroxyl and superoxide anion radicals, chelating ability of iron ion and reducing power. Results of antioxidant assays indicated that the UDPSPY had higher antioxidant activity of scavenging superoxide anion radical and hydroxyl radical than that of the PSPY. As antioxidant activities of the fractions are concerned, this result might suggest that decreasing molecular weight and increasing sulfate group content in the molecule were vital and necessary factors for improving antioxidant activity of PSPY. Decrease of molecular weight was the dominant reason for increasing activities of scavenging non-site and site specific hydroxyl radical and reducing power of F1, F2, and F3, and increase of sulfate group content in the molecule was the dominant reason for increasing activities of scavenging superoxide anion radical and chelating ferrous ions of F1, F2, and F3. It was possible that ultrasonic treatment could be a new and effective method for enhancing PSPY's antioxidant activity.

Antioxidant and ROS inhibitory activities of heterocyclic 2-Aryl-4(3H)-quinazolinone derivatives

2021 ◽  
Vol 18 ◽  
Author(s):  
Shahida Perveen ◽  
Syed Muhammad Saad ◽  
Khalid Mohammed Khan ◽  
Muhammad Iqbal Choudhary
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Cell Line ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Radical Scavenging ◽  
Superoxide Anion Radical ◽  
Inhibitory Activities ◽  
Quinazolinone Derivatives

Background: Antioxidants are small molecules that prevent or delay the process of oxidations caused by highly reactive free radicals. These molecules are known for their ability to protect various cellular architecture and other biomolecules from oxidative stress and free radicals. Thus, antioxidants play a key role in the prevention of oxidative damages caused by highly reactive free radicals. Method: In the present study, a series of previously synthesized heterocyclic 2-aryl-4(3H)-quinazolinone derivatives 1-25 was screened for antioxidant activity by employing in vitro DPPH and superoxide anion radical scavenging activities. ROS inhibitory activities were also evaluated by serum-opsonized zymosan activated whole blood phagocytes and isolated neutrophils. Cytotoxicity studies were carried out by employing an MTT assay against the 3T3 cell line. Results: Most of the 2-aryl-4(3H)-quinazolinone derivatives showed potent antioxidant activities in superoxide anion radical scavenging assay with IC50 value ranging between 0.57 µM – 48.93 µM, as compared to positive control quercetin dihydrate (IC50 = 94.1± 1.1 µM ). Compounds 5, 6, and 14 showed excellent activity in DPPH assay. Compounds 5-8, 12-15, 17, and 20 showed promising activities in the ROS inhibition assay. All compounds were found to be non-cytotoxic against the 3T3 cell line. Structure antioxidant activity has been established. Conclusion: It can be concluded that most of the heterocyclic 2-aryl-4(3H)-quinazolinone derivatives 1-25 are identified as promising antioxidant agents that are capable of fighting against free radicals and oxidative stress. Thus, they can serve as a lead towards treating oxidative stress and related pathologies.

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