scholarly journals In vitro micropropagation of banana

1970 ◽  
Vol 34 (4) ◽  
pp. 645-659 ◽  
Author(s):  
MD Al-Amin ◽  
MR Karim ◽  
MR Amin ◽  
S Rahman ◽  
ANM Mamun
Keyword(s):  
Room Temperature ◽  
Root Formation ◽  
Agricultural Research ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Root Numbers ◽  
Half Strength ◽  
Number Of Leaves ◽  
Different Levels

The present study was conducted at the Biotechnology Laboratory, Biotechnology Division, Bangladesh Agricultural Research Institute (BARI), Gazipur during the period from September 2004 to June 2005 to investigate the effect of different concentrations of BAP and NAA on virus free plant regeneration, shoot multiplication and different concentrations of IBA and IAA on in vitro root formation of banana cv. BARI Banana-I. The culture meristem first turned brown in colour in 4-5 days which grew into a green globular hard coat mass after 30-35 days. From this ball like structure, adventitious plantlets were developed. Among the different concentrations, 7.5 mg/l BAP + 0.5 mg/l NAA showed highest shoot proliferation of 0.75, 2.75 and 6.25 shoots per explant at 10, 20 and 30 DAI, respectively. The longest shoot (1.03, 2.45 and 3.38 cm) at 10, 20 and 30 DAI, respectively, was produced by the treatment combination of 7.5 mg/l BAP + 0.5 mg/l NAA. The maximum number of leaves (2.50, 3.25 and 7.00 leaves/explant at 10, 20 and 30 DAI) were produced on the medium supplemented with the same treatment and it also produced the longest leaves, 0.85, 2.70 and 4.23 cm at 10, 20 and 30 DAI, respectively. For root initiation half strength MS medium supplemented with different levels of IBA (0, 0.5, 1 .0 and 1.50 mg/l) and IAA (0, 0.5 and 1.0 mg/l) was used. Root numbers varied with different concentrations of IBA and IAA. The highest number of roots were produced by 0.5 mg/l IAA + 0.5 mg/l IBA. The highest length (2.93, 4.63 and 5.88 cm) was recorded at 10, 20 and 30 DAI in the same treatment which was statistically significant. Meristem derived plantlets were transferred to poly bags containing 1:1 (ground soil : cowdung) mixture after 7 days hardening in room temperature (28-30°C) and established plantlet was ready for planting. Key Words: Banana; regeneration; micropropagation; plantlet.  DOI: 10.3329/bjar.v34i4.5840Bangladesh J. Agril. Res. 34(4) : 645-659, December 2009

scholarly journals Micropropagation of Eclipta alba (Linn.) Hassk- a Valuable Medicinal Herb

1970 ◽  
Vol 43 (2) ◽  
pp. 215-222 ◽  
Author(s):  
AKM Sayeed Hassan ◽  
Farhana Afroz ◽  
Laila Shamroze Bari ◽  
John Liton Munshi ◽  
Miskat Ara Akhter Jahan ◽  
...  
Keyword(s):  
Room Temperature ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Medicinal Herb ◽  
Mass Propagation ◽  
Eclipta Alba ◽  
Half Strength ◽  
Regenerated Plantlets

A protocol was established for mass propagation of a valuable medicinal herb, Eclipta alba (Linn.) Hassk (Asteraceae) through in vitro culture. Apical and axillary buds of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mgl-1 BAP + 0.1 mgl-1 NAA, in which 94% of the explants produced 18 shoots per culture. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 26 shoots per culture. In vitro raised shoots rooted on half strength MS medium with 1.0 mgl-1 IBA +1.0 mgl-1 NAA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 80%. Key words: Eclipta alba, Medicinal plant, Shoot proliferation, Micropropagation, Acclimatization   DOI: 10.3329/bjsir.v43i2.965 Bangladesh J. Sci. Ind. Res. 43(2), 215-222, 2008 

scholarly journals In Vitro Mass Propagation of Heliotropium indicum L., using Apical and Axillary Bud Explants

1970 ◽  
Vol 45 (1) ◽  
pp. 69-74 ◽  
Author(s):  
AKM Sayeed Hassan ◽  
Nadira Begum ◽  
Miskat Ara Akhter Jahan ◽  
Rahima Khatun
Keyword(s):  
Room Temperature ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Axillary Buds ◽  
Mass Propagation ◽  
Half Strength ◽  
Heliotropium Indicum ◽  
Regenerated Plantlets

A consequency was obtained for mass propagation of a valuable ayurvedic medicinal herb, Heliotropium indicum Linn. (Boraginaceae) through in vitro culture. Apical and axillary buds of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP + 0.1 mg/l GA3, in which 92% of the axillary buds explants produced 12 shoots per culture. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 18 shoots per culture. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IBA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%. Key words: Heliotropium indicum; Medicinal plant; Shoot proliferation; Micropropagation; Acclimatization. DOI: 10.3329/bjsir.v45i1.5185 Bangladesh J. Sci. Ind. Res. 45(1), 69-74, 2010

scholarly journals In vitro Shoot Proliferation and Plant Regeneration of Phlogacanthus thyrsiflorus Nees. a Rare Medicinal Shrub of Bangladesh

2011 ◽  
Vol 21 (2) ◽  
pp. 135-141 ◽  
Author(s):  
A.K. M. Sayeed Hassan ◽  
Nadira Begum ◽  
Rebeka Sultana ◽  
Rahima Khatun
Keyword(s):  
Survival Rate ◽  
Plant Regeneration ◽  
Room Temperature ◽  
Shoot Proliferation ◽  
Nodal Explants ◽  
Shoot Induction ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
In Vitro Shoot Proliferation

An efficient protocol was developed for shoot proliferation and plant regeneration of Phlogacanthus thyrsiflorus Nees. (Acanthaceae) - a rare medicinal shrub of Bangladesh, through in vitro culture using shoot tip and nodal explants. Best shoot induction was observed on MS with 1.0 mg/l BAP + 0.5 mg/l NAA, in which 84.2% of nodal explants responded to produce maximum number (12.4 ± 0.66) of shoots per culture. In vitro raised shoots rooted on half-strength MS with 0.5 mg/l IBA + 0.5 mg/l NAA. For acclimation and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%. Key words: Phlogacanthus thyrsiflorus, Shoot proliferation, Plant regeneration   D. O. I. 10.3329/ptcb.v21i2.10236   Plant Tissue Cult. & Biotech. 21(2): 135-141, 2011 (December)

scholarly journals Effect of different levels of NAA on in vitro growth and development of shoots of Dendrobium orchid

1970 ◽  
Vol 34 (3) ◽  
pp. 411-416 ◽  
Author(s):  
MS Parvin ◽  
ME Haque ◽  
F Akhter ◽  
M Moniruzzaman ◽  
ABM Khaldun
Keyword(s):  
Growth And Development ◽  
Shoot Proliferation ◽  
Shoot Length ◽  
In Vitro Growth ◽  
Shoot Number ◽  
Number Of Leaves ◽  
Shoot Weight ◽  
Different Levels ◽  
In Vitro Shoot Proliferation

The present study was conducted to investigate the effect of growth regulator NAA on in vitro shoot proliferation, rooting, and plantlet establishment. Among the different concentrations of NAA, the best increase in shoot weight (0.25 g) and shoot number (8.83) were observed from 0.1 mg/I NAA. The highest shoot length (2.60 cm), number of leaves (4.83), number of roots (5.15), and root length (2.67 cm) were obtained with 0.2 mg/I NAA at 60 DAT. Key Words: Dendrobium orchid, NAA, MS media. DOI: 10.3329/bjar.v34i3.3966 Bangladesh J. Agril. Res. 34(3) : 411-416, September 2009

scholarly journals In vitro Regeneration through Apical and Axillary Shoot Proliferation of Ficus religiosa L. - A Multi-purpose Woody Medicinal Plant

2010 ◽  
Vol 19 (1) ◽  
pp. 71-78 ◽  
Author(s):  
A.K.M. Sayeed Hassan ◽  
Farhana Afroz ◽  
Miskat Ara Akhter Jahan ◽  
Rahima Khatun
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Axillary Shoot ◽  
Axillary Shoot Proliferation ◽  
Ficus Religiosa ◽  
Half Strength

A protocol was established for mass propagation of the valuable medicinal plant Ficus religiosa L. (Moraceae) through in vitro culture using apical and axillary buds of young sprouts from selected plants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP + 0.1 mg/l IAA, in which 78 per cent of the explants produced 16 shoots per culture. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 24 shoots per culture. In vitro raised shoots rooted on half strength MS supplemented with 2.0 mg/l IBA + 0.1 mg/l NAA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for seven days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85 per cent.  Key words: Ficus religiosa, Medicinal plant, Shoot proliferation, Regeneration,                   Acclimatization D.O.I. 10.3329/ptcb.v19i1.4987 Plant Tissue Cult. & Biotech. 19(1): 71-78, 2009 (June)

scholarly journals 027 MULTIPLICATION OF ROSE SPECIES IN VITRO

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 431d-431
Author(s):  
Yan Ma ◽  
David H. Byrne ◽  
Jing Chen ◽  
Amanda Byrne
Keyword(s):  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Naphthalene Acetic Acid ◽  
Good Growth ◽  
Lateral Buds ◽  
Half Strength ◽  
The Media

Several rose species (Rosa rugosa, R. wichuraiana, R. setigera, R. laevigata, R. banksiae, R. roxburghii, R. odorata and hybrids) were employed to establish the appropriate nutrient media for shoot multiplication and root initiation of cultured shoots and to describe a procedure for the successful transfer to soil of plants obtained in vitro. Cultured shoot tips and lateral buds from different genotypes proliferated multiple shoots on a basal medium (MS salt, vitamins, glycine, sucrose and agar) supplemented with 0mg/l to 6mg/l 6-benzylamino purine (BA) and 0mg/l to 0.5 mg/l naphthalene acetic acid (NAA). Most rose species cultured in a modified MS medium supplemented with 2mg/l BA showed good growth and shoot proliferation. The buds nearest the apex exhibited the slowest rate of bud development. Root development was enhanced and shoot development inhibited by lowering the concentration of MS salts to quarter- and half-strength. With difficult-to-root species, rooting was improved by supplementing the media with auxin or giving them 3-7days of dark treatment.

scholarly journals Pyroligneous Acid Improves In Vitro Rooting of Japanese Pear Cultivars

HortScience ◽  
2002 ◽  
Vol 37 (1) ◽  
pp. 194-195 ◽  
Author(s):  
Masanori Kadota ◽  
Takashi Hirano ◽  
Kiyotoshi Imizu ◽  
Yoshiji Niimi
Keyword(s):  
Root Formation ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
In Vitro Rooting ◽  
Japanese Pear ◽  
Pyrus Pyrifolia ◽  
Shoot Cultures ◽  
Pyroligneous Acid ◽  
In Vitro Shoot Proliferation

Effects of PA on in vitro shoot proliferation and root formation were investigated using shoot cultures of three Japanese pear (Pyrus pyrifolia Nakai) cultivars. PA inhibited shoot multiplication and promoted initiation and development of roots in the cultured shoots of three cultivars, resulting in increasing the proportion of rooted shoots. Chemical name used: pyroligneous acid (PA).

Nutrient responses differ between node and organogenic cultures of Corymbia torelliana×C. citriodora (Myrtaceae)

2010 ◽  
Vol 58 (5) ◽  
pp. 410 ◽  
Author(s):  
Cao Dinh Hung ◽  
Stephen J. Trueman
Keyword(s):  
Root Formation ◽  
Shoot Proliferation ◽  
Shoot Elongation ◽  
Plantation Establishment ◽  
Full Strength ◽  
Eastern Australia ◽  
Half Strength ◽  
Strength Medium ◽  
Node Culture

Hybrids between Corymbia torelliana (F.Muell.) K.D.Hill & L.A.S.Johnson and C. citriodora subsp. variegata (F.Muell.) A.R.Bean & M.W.McDonald are used extensively for plantation forestry in subtropical eastern Australia; however, plantation establishment has been hampered by inadequate seed supply and variable amenability to propagation as rooted cuttings. The present study investigated node-culture and organogenic-culture methods for in vitro propagation of two families of juvenile Corymbia hybrids. The effects of nutrient and auxin concentrations on root formation, shoot elongation and shoot proliferation, and subsequent conversion to plantlets in an in vitro soil-less system, were assessed. The response to the nutrient concentration differed between organogenic and node cultures. Half-strength, compared with full-strength, medium with auxin increased shoot proliferation by 58% for one family in the node culture, in which many shoots form roots and elongate rapidly in the absence of cytokinin. However, full-strength medium increased shoot proliferation by 213% over that in the half-strength medium for the other family in the organogenic culture, in which shoots do not form roots in the presence of cytokinin, and nutrient uptake occurs via callus and stem tissue rather than roots. The auxin, naphthalene acetic acid, sometimes stimulated root formation and shoot elongation in the node culture, although it had no effect on the final number of shoots. Most shoots (57–100%) subsequently formed roots in the in vitro soil-less medium. The high rates of proliferation and plantlet conversion in the present study provide the means, not only for simultaneous laboratory storage and field-testing of Corymbia clones before selection of desired genotypes, but also for en masse plantlet production of selected clones or families for plantation establishment.

scholarly journals In vitro Shoot Proliferation and Plant Regeneration of Physalis minima L. - a Perennial Medicinal Herb

2010 ◽  
Vol 44 (4) ◽  
pp. 453-456 ◽  
Author(s):  
Farhana Afroz ◽  
AKM Sayeed Hassan ◽  
Laila Shamroze Bari ◽  
Rebeka Sultana ◽  
Nadira Begum ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Room Temperature ◽  
Shoot Proliferation ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Ms Medium ◽  
Regenerated Plants ◽  
Half Strength ◽  
Shoot Tip Explants

The present study describes a protocol for high frequency plant regeneration of Physalis minima. Shoots were induced by culturing nodal segments and shoot tips from 15 day old seedlings. About 29 and 32 shoots were found to be induced from nodal segment and shoot tip explants, respectively, cultured on MS medium supplemented with 1.0 mg/l BAP. When shoots were subcultured on the fresh medium with same component as mentioned above, the shoots were elongated. Shoots rooted well when they were excised individually and implanted on half-strength MS medium with 0.3 mg/l NAA, where 98% shoots rooted within 12-15 days. In vitro grown plantlets with strong root system were successfully established in normal room temperature for seven days before transplanting in pots where they were reared for three weeks through successive acclimatization. The regenerated plants were successfully transferred to the soil with 90% survival rate. Key words: Physalis minima; Medicinal plant; Shoot proliferation; Micropropagation; Regeneration DOI: 10.3329/bjsir.v44i4.4597 Bangladesh J. Sci. Ind. Res. 44(4), 453-456, 2009

scholarly journals High Frequency In Vitro Regeneration of Dianthus caryophyllus L., a Herbaceous Perennial Ornamental Plant

1970 ◽  
Vol 46 (4) ◽  
pp. 495-498 ◽  
Author(s):  
AKM Sayeed Hassan ◽  
JL Munshi ◽  
R Sultana ◽  
MAA Jahan ◽  
R Khatun
Keyword(s):  
High Frequency ◽  
Room Temperature ◽  
Dianthus Caryophyllus ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Ornamental Plant ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
Herbaceous Perennial

High frequency plant regeneration was established from shoot tips and nodal explants of a perennial ornamental plant, Dianthus caryophyllus L. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP + 0.1 mg/l NAA, in which 82% of the explants responded to produce maximum number of shoots (38) per culture. In vitro raised healthy shoots were rooted on half strength MS medium with 0.5 mg/l IBA +0.5 mg/l NAA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 78%. Key words: Dianthus caryophyllus; Shoot proliferation; Micropropagation; Acclimatization DOI: http://dx.doi.org/10.3329/bjsir.v46i4.9597 BJSIR 2011; 46(4): 495-498

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