scholarly journals Efisiensi Mikropropagasi Pisang Kepok Amorang melalui Modifikasi Formula Media dan Temperatur

2016 ◽  
Vol 6 (2) ◽  
pp. 91
Author(s):  
Yati Supriati
Keyword(s):  
In Vitro Culture ◽  
Incubation Temperature ◽  
Culture Media ◽  
Shoot Multiplication ◽  
Root Induction ◽  
Incubation Condition ◽  
Growth Environment ◽  
Media Formulation ◽  
The Media

<p>Micropropagation Efficiency of Banana cv Kepok<br />Amorang through Modifications of Culture Media and<br />Incubation Temperature. Yati Supriati. The budless<br />banana cv Kepok Amorang is potentially commercialized<br />due to its sweet taste and does not have flower bud, hence<br />reduced the potential of being infected by the blood disease<br />pathogen. Enhancement of banana industry needs continuous<br />supplies of large number banana seedlings. In vitro<br />culture enable the production of seedlings in a large scale,<br />uniform, quick. The research aims: (1) to formulate an<br />efficient medium for in vitro multiplication of cv Kepok<br />Amorang shoot, (2) to identify efficient growth environment<br />for in vitro culture of cv Kepok Amorang, and (3) to formulate<br />an efficient culture medium for roots inductions of cv<br />Kepok Amorang. The plant material used was in vitro culture<br />of Kepok cv Amorang, 2 cm in height without leaf and root.<br />The media formulation for shoot multiplication were full<br />strength, half strength, one fourth strength MS media,<br />supplemented with either 1, 3, or 5 ppm IBA. On optimization<br />step, the media tested were MS, Knop, Knop and<br />Heller, Hyponex N, Growmore N, and Rosasol N containing<br />of 1 ppm BA. The explants were incubated in culture room<br />with 8, 12, and 16 hours photoperiod with temperatures 30oC<br />(non air conditioned) and 25oC (air conditioned). The root<br />induction trial was done using MS, Knop, Knop and Heller,<br />Hyponex N, Growmore N, and Rosasol N media containing<br />of 1 ppm and 3 ppm IBA. The results showed that the best<br />medium formula for shoot multiplication was ¼ MS + 1 ppm<br />IBA. The best incubation condition was 16 hours photoperiods<br />at 30oC. The best media for root induction was<br />Hyponex 2 g/l + 1 ppm IBA. This culture method reduced<br />cost by Rp 261.7 per plantlet through efficiency of media<br />formulation and electricity use.</p>

In vitro culture of pointed gourd (Trichosanthes dioica Roxb.)

1970 ◽  
Vol 35 (1) ◽  
pp. 135-142 ◽  
Author(s):  
MA Malek ◽  
D Khanam ◽  
M Khatun ◽  
MH Molla ◽  
MA Mannan
Keyword(s):  
Plant Regeneration ◽  
In Vitro Culture ◽  
Culture Media ◽  
Culture Conditions ◽  
Root Induction ◽  
Ms Medium ◽  
Trichosanthes Dioica ◽  
Pointed Gourd ◽  
Regenerated Plantlets

An experiment was conducted to study the in vitro culture of pointed gourd. Cotyledon rescued from physiologically matured seeds (PMS) and immatured seeds (IMS) of pointed gourd were used as explants. Cotyledon excised from PMS responded very well in all culture conditions. Plant regenerated from cotyledon of PMS ranged from 38 to 96% in different hormonal formulations of culture media. Highest percentage of shoot regeneration was observed in MS + 1.0 mg/l BAP and lowest in MS + 2.5 mg/l BAP. No plant regeneration was observed in cotyledon from immatured seeds. The highest percentage of root induction (99%) was achieved in half MS medium supplemented with 0.5 mg/l NAA. The regenerated plantlets were successfully established in earthen pot. Keywords: Cotyledon; in vitro; pointed gourd. DOI: 10.3329/bjar.v35i1.5874Bangladesh J. Agril. Res. 35(1) : 135-142, March 2010

scholarly journals Histology of Microspores of Chile Apple Capsicum pubescens R and P. and in vitro Culture

2021 ◽  
Vol 2 (7) ◽  
pp. 01-06
Author(s):  
J. L. Rodríguez-de la O ◽  
F. Pérez-Pérez ◽  
M. Pérez-Grajales
Keyword(s):  
In Vitro Culture ◽  
Culture Media ◽  
Plant Biotechnology ◽  
Pollen Grains ◽  
Dichlorophenoxyacetic Acid ◽  
The Media ◽  
Pure Lines ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Short Time

In plant biotechnology, in vitro culture of gametic or sexual cells, microspores or pollen grains, has been described as a successful tool to accelerate genetic improvement, obtaining haploid, homozygotic plants or pure lines in a short time. In chile apple, Capsicum pubescens R and P. Anthers were sown in vitro, and their cytological analysis, locating the meiotic division stage of microspores or pollen grains. Flower buds with diameters from 2.5 to 4.4 mm were pre-incubated at 4°C, in ascorbic and citric acid at 100 and 150 mg-L-1 for 24 h. Five semisolid culture media (A1, A2, A3, A4 and A5) were used, with Murashige and Skoog (1962) salts (MS), modifying iron and vitamin chelates, sucrose, and L-cysteine, 2,4-dichlorophenoxyacetic acid (2,4-D) and Kinetin (Kin). Anthers, in vitro, were plated, in light and dark, for 70 days. Two differentiation media (R1 and R2) were evaluated with 100% MS salts, glycine, kinetin and myo-inositol. The anthers seeded, coincided with the first mitosis of the microspore, the anthers, formed callus in the media (A1) 100 % EDTA-Fe, 0.40 mg-L-1 thiamine, 3 % sucrose) and (A3) 100 % EDTA-Fe, 0.40 mg-L-1 thiamine, 3 % sucrose, 0. 3 mg-L-1 of 2,4-D, and differentiated pro-embryonic structures in (A3) and (A5) 200 % EDTA-Fe, 0.4 mg-L-1 thiamine, 50 mg-L-1 pyridoxine, folic acid, riboflavin and niacin, 0.3 mg-L-1 2,4-D plus 0.3 mg-L-1 Kinetin, as well as roots in (A1). Light influenced the formation of pro-embryos and roots, in the dark callus. The media (R1) and (R2) favored the formation of pro-embryos.

scholarly journals APLIKASI SITOKININ TIPE PURIN DAN UREA PADA MULTIPLIKASI TUNAS ANIS (Pimpinellla anisum L.) IN VITRO

2020 ◽  
Vol 13 (1) ◽  
pp. 1
Author(s):  
OTIH ROSTIANA
Keyword(s):  
Essential Oils ◽  
Culture Media ◽  
Shoot Multiplication ◽  
Tropical Region ◽  
Herbaceous Plant ◽  
Solid Culture ◽  
Pimpinella Anisum ◽  
The Media ◽  
Initiation Stage

ABSTRAK<br />Anis (Pimpinella anisum L.) merupakan tanaman herba tahunan<br />yang termasuk ke dalam famili Umbelliferae. Buahnya diketahui<br />mengandung minyak atsiri yang didominasi senyawa trans-anethol (90%)<br />dan berkhasiat sebagai antiseptik, antispasmodik, antikanker, karminatif,<br />pelega tenggorokan, obat bronkitis, serta digunakan dalam pembuatan<br />sabun, parfum, pasta gigi, juga krim kulit. Sebagai tanaman bernilai<br />ekonomi, upaya perbanyakan anis perlu dilakukan. Perbanyakan secara in<br />vitro dengan teknik kultur jaringan merupakan salah satu metode alternatif<br />yang dapat digunakan untuk menghasilkan bibit dalam jumlah banyak,<br />seragam dan dalam waktu yang relatif singkat. Dengan penambahan<br />sitokinin sintetik tipe urea seperti thidiazuron (TDZ) dan tipe purin seperti<br />benzil amino purin (BAP) akan memacu inisiasi dan proliferasi tunas.<br />Penelitian ini bertujuan mendapatkan media yang tepat untuk menginduksi<br />tunas anis yang optimal dengan penambahan BAP atau TDZ, mengetahui<br />respon pertumbuhan dan penampakan kultur akibat penambahan berbagai<br />konsentrasi BAP atau TDZ, serta mempelajari sinergisme yang terjadi<br />antara keduanya. Pada tahap inisiasi, eksplan berupa tunas pucuk diinduksi<br />di dalam media MS padat dengan penambahan BAP (0,1 mg/l; 0,2 mg/l;<br />0,3 mg/l; 1 mg/l; 2 mg/l; 3 mg/l), atau TDZ dengan kisaran konsentrasi<br />yang sama. Tunas terbanyak yang dihasilkan dari dua jenis sitokinin pada<br />tahap ini disubkultur ke dalam media yang ditambahkan jenis sitokinin<br />yang berbeda (TDZ ke BAP atau BAP ke TDZ) pada konsentrasi 0,3 mg/l<br />atau 3 mg/l. Pada media yang ditambahkan TDZ dihasilkan tunas anis<br />lebih banyak (3,62-6,28) dibandingkan pada media yang ditambahkan<br />BAP (1,86-2,78), tetapi tunas yang dihasilkan pendek (roset). Sedangkan<br />tunas yang dihasilkan dalam media yang ditambahkan BAP beruas lebih<br />tinggi tetapi jumlah tunasnya sedikit. Subkultur tunas anis ke dalam media<br />yang diperkaya dengan sitokinin yang berbeda meningkatkan jumlah tunas<br />yang berproliferasi dan memperbaiki visual tunas.<br />Kata kunci: Anis, Pimpinellla anisum L. ,  minyak atsiri, multiplikasi tunas,<br />in vitro, TDZ, BAP, Jawa Barat<br />ABSTRACT<br />Application of purine and urea types of cytokinins in<br />shoot multiplication of Anise (Pimpinella anisum L.) in<br />vitro<br />Pimpinella anisum L. or sweet anise is an annual–herbaceous plant<br />belongs to the Umbelliferae family. The fruit of anise contains of essential<br />oil, which is mainly consisted of trans-anethol (90%). Essential oils of<br />anise is mainly used as an antiseptic, antispasmodic, anticancer,<br />carminative, expectorant and has also been used as component in soap,<br />perfumery, tooth paste, and skin cream productions. Since this crop is<br />mainly cultivated in sub tropical region, anise cultivation in Indonesia has<br />not been performed. To obtain sufficient numbers of anise planting<br />materials in vitro propagation was conducted by applying benzyl amino<br />purine (BAP) and thidiazuron (TDZ). In this research TDZ or BAP were<br />applied at various concentrations (0,1 mg/l: 0.2 mg/l; 0.3 mg/l; 1 mg/l; 2<br />mg/l; 3 mg/l), to induce shoots in MS-solid culture media. The highest<br />number of shoots obtained in those two type of cytokinins containing<br />media from the initiation stage were subcultured into the media<br />supplemented with different cytokinins (TDZ to BAP or BAP to TDZ) at<br />0.3 mg/l or 3 mg/l levels. The results showed that medium with the<br />addition of TDZ resulted in higher numbers of shoot (3,26-6,28) than that<br />of medium with an addition of BAP (1,86-2,78). However, rosette shoots<br />were dominant in TDZ containing medium. On the other hand, medium<br />with an addition of BAP resulted in less numbers of shoots with taller<br />nodes. Subculture of anise into different kinds of cytokinins increased the<br />numbers of proliferated-shoots and recovered the abnormal shoots.<br />Key words : Anise, Pimpinellla anisum L, essential oils, shoots<br />multiplication, in vitro, TDZ, BAP, West Java

scholarly journals Perbanyakan Tanaman Jambu Mete (Anacardium occidentale L.) melalui Jalur Organogenesis

2016 ◽  
Vol 8 (3) ◽  
pp. 113
Author(s):  
Rossa Yunita ◽  
Ika Mariska ◽  
Christiani Tumilisar
Keyword(s):  
In Vitro Culture ◽  
Adventitious Shoots ◽  
Shoot Multiplication ◽  
Medium Composition ◽  
Anacardium Occidentale ◽  
Root Induction ◽  
Shoot Induction ◽  
Lateral Shoots

<p>Vegetative<br />propagation through in vitro culture has been carried out as<br />a technology that has the potential for obtaining seedling in<br />significant amounts and relatively faster. This activity can be<br />done through the multiplication of adventitious shoots and<br />lateral shoots (organogenesis). The goal of this research was<br />to find the method of cashew micropropagation through<br />organogenesis. This study consisted of 4 main activities.<br />They were shoot induction, shoot multiplication, shoot<br />elongation, and root induction. The results showed the best<br />medium composition for shoot induction was MS + BA 0.7<br />mg/l. The suitable media for shoots multliplication was MS +<br />thidiazuron 0.5 mg/l + zeatin 1 mg/l and for shoots<br />elongation was MS + GA 1 mg/l + zeatin + 3 mg/l. The best<br />methods for root induction was by submerging in vitro<br />shoots in a solution of IAA 100 mg/l.</p>

scholarly journals Rheological and Microstructural Features of Plant Culture Media Doped with Biopolymers: Influence on the Growth and Physiological Responses of In Vitro-Grown Shoots of Thymus lotocephalus

2021 ◽  
Vol 2 (2) ◽  
pp. 538-553
Author(s):  
Natacha Coelho ◽  
Alexandra Filipe ◽  
Bruno Medronho ◽  
Solange Magalhães ◽  
Carla Vitorino ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Culture Medium ◽  
Culture Media ◽  
Average Pore Size ◽  
Physiological Responses ◽  
Gum Arabic ◽  
Shoot Elongation ◽  
Hydroxyethyl Cellulose ◽  
Plant Culture

In vitro culture is an important biotechnological tool in plant research and an appropriate culture media is a key for a successful plant development under in vitro conditions. The use of natural compounds to improve culture media has been growing and biopolymers are interesting alternatives to synthetic compounds due to their low toxicity, biodegradability, renewability, and availability. In the present study, different culture media containing one biopolymer (chitosan, gum arabic) or a biopolymer derivative [hydroxyethyl cellulose (HEC), carboxymethyl cellulose (CMC)], at 100 or 1000 mg L−1, were tested regarding their influence on the growth and physiological responses of Thymus lotocephalus in vitro culture. Cellulose-based biopolymers (HEC and CMC) and gum arabic were used for the first time in plant culture media. The results showed that CMC at 100 mg L−1 significantly improved shoot elongation while chitosan, at the highest concentration, was detrimental to T. lotocephalus. Concerning only the evaluated physiological parameters, all tested biopolymers and biopolymer derivatives are safe to plants as there was no evidence of stress-induced changes on T. lotocephalus. The rheological and microstructural features of the culture media were assessed to understand how the biopolymers and biopolymer derivatives added to the culture medium could influence shoot growth. As expected, all media presented a gel-like behaviour with minor differences in the complex viscosity at the beginning of the culture period. Most media showed increased viscosity overtime. The surface area increased with the addition of biopolymers and biopolymer derivatives to the culture media and the average pore size was considerably lower for CMC at 100 mg L−1. The smaller pores of this medium might be related to a more efficient nutrients and water uptake by T. lotocephalus shoots, leading to a significant improvement in shoot elongation. In short, this study demonstrated that the different types of biopolymers and biopolymer derivatives added to culture medium can modify their microstructure and at the right concentrations, are harmless to T. lotocephalus shoots growing in vitro, and that CMC improves shoot length.

Biochemical markers for pregnancy in the spent culture medium of in vitro produced bovine embryos

2021 ◽  
Author(s):  
Gabriela de Oliveira Fernandes ◽  
Marcella Pecora Milazzotto ◽  
Andrei Antonioni Guedes Fidelis ◽  
Taynan Stonoga Kawamoto ◽  
Ligiane de Oliveira Leme ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Biochemical Markers ◽  
Culture Medium ◽  
Culture Media ◽  
Ionization Mass ◽  
Metabolic Profiles ◽  
Bovine Embryos ◽  
The Media

Abstract The present study aimed to identify biomarkers to assess the quality of in vitro produced (IVP) bovine embryos in the culture media. IVP embryos on Day (D) 5 of development were transferred to individual drops, where they were maintained for the last 48 h of culture. Thereafter, the medium was collected and the embryos were transferred to the recipients. After pregnancy diagnosis, the media were grouped into the pregnant and nonpregnant groups. The metabolic profiles of the media were analyzed via electrospray ionization mass spectrometry, and the concentrations of pyruvate, lactate, and glutamate were assessed using fluorimetry. The spectrometric profile revealed that the media from embryos from the pregnant group presented a higher signal intensity compared to that of the nonpregnant group; the ions 156.13 Da [M + H]+, 444.33 Da [M + H]+, and 305.97 Da [M + H]+ were identified as biomarkers. Spent culture medium from expanded blastocysts (Bx) that established pregnancy had a greater concentration of pyruvate (p = 0.0174) and lesser concentration of lactate (p = 0.042) than spent culture medium from Bx that did not establish pregnancy. Moreover, pyruvate in the culture media of Bx can predict pregnancy with 90.9% sensitivity and 75% specificity. In conclusion, we identified markers in the culture media that helped in assessing the most viable IVP embryos with a greater potential to establish pregnancy.

scholarly journals In vitro culture as an aid to conservation of indigenous ferns: Diplazium proliferum

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Zubeir M. Golamaully ◽  
Vishwakalyan Bhoyroo ◽  
Nadeem Nazurally ◽  
Vineshwar Gopal
Keyword(s):  
Growth Rate ◽  
In Vitro Culture ◽  
Mercuric Chloride ◽  
Rare Species ◽  
Vascular Plants ◽  
Culture Media ◽  
Fungal Contamination ◽  
Increase Growth Rate ◽  
Growing Population

With the ever growing population and economic needs of Mauritius, the flora of Mauritius has never been in more danger and one group of vascular plants is even more in peril; ferns.<em> Diplazium proliferum</em> is indigenous to the Mascarene region and is considered as a rare species in Mauritius. The need to develop a tested <em>in vitro</em> propagation protocol is a must to protect the biodiversity of Mauritius. This experiment was geared towards the establishment of a proper sterilization technique and the effect of 6-benzylaminopurine (BAP) and light on <em>in vitro</em> culture of this fern. Sterilization with 0.05% Mercuric chloride was effective to eliminate fungal contamination and allow germination of spores. Culture media supplemented with BAP did not significantly increase growth rate of both gametophytes and sporophytes of<em> D. proliferum</em>. Present results suggest efficient sterilization methods to be a crucial stage for successful<em> in vitro r</em>egeneration of ferns. The established protocol will be used as an optimized baseline protocol for the propagation of other indigenous ferns.

scholarly journals In Vitro Micropropagation of the Medicinal Plant Physalis angulata L.

2016 ◽  
Vol 8 (2) ◽  
pp. 161-163
Author(s):  
Owk ANIEL KUMAR ◽  
Songa RAMESH ◽  
Sape SUBBA TATA
Keyword(s):  
Large Scale ◽  
Leaf Disc ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Medicinal Herb ◽  
Root Induction ◽  
Survival Percentage ◽  
Physalis Angulata

Physalis angulata L. is an important medicinal herb. An efficient direct adventitious plant regeneration protocol was developed for large scale propagation using leaf disc as explants. The explants were cultured on MS basal medium supplemented with 0.25-3.0 mg/L 6-benzyl amino purine (BAP) for primary shoot proliferation. Inclusion of indole-3-acetic acid (IAA) and gibberellic acid (GA3) in the culture medium along with BAP promoted a higher rate of shoot multiplication. The maximum number of shoots was produced in MS + BAP (1.0 mg/L) + IAA (0.5 mg/L) + GA3 (0.20 mg/L) after the third subculture. An average of 152.8 ± 0.40 shoots were produced from each leaf disc. For root induction the shootlets were transferred to MS medium supplemented with different concentrations of indole-3-butyric acid (IBA). The highest percentage of root induction was observed in 1.0 mg/L (IBA). Rooted plants were successfully established in the soil after hardening. The survival percentage of rooted plants on soil was found to be 85%. This result will facilitate the conservation and propagation of the important medicinal herb Physalis angulata L.

scholarly journals Adjustment of Mineral Elements in the Culture Medium for the Micropropagation of Three Vriesea Bromeliads from the Brazilian Atlantic Forest: The Importance of Calcium

HortScience ◽  
2009 ◽  
Vol 44 (1) ◽  
pp. 106-112 ◽  
Author(s):  
Alice Noemí Aranda-Peres ◽  
Lázaro Eustáquio Pereira Peres ◽  
Edson Namita Higashi ◽  
Adriana Pinheiro Martinelli
Keyword(s):  
New Media ◽  
Mineral Composition ◽  
Culture Media ◽  
Dry Weight ◽  
Defined Medium ◽  
Ms Medium ◽  
Media Formulation ◽  
Half Strength

Many different species of Bromeliaceae are endangered and their conservation requires specific knowledge of their growth habits and propagation. In vitro culture of bromeliads is an important method for efficient clonal propagation and in vitro seed germination can be used to maintain genetic variability. The present work aims to evaluate the in vitro growth and nutrient concentration in leaves of the epiphyte bromeliads Vriesea friburguensis Mez, Vriesea hieroglyphica (Carrière) E. Morren, and Vriesea unilateralis Mez, which exhibit slow rates of growth in vivo and in vitro. Initially, we compared the endogenous mineral composition of bromeliad plantlets grown in half-strength Murashige and Skoog (MS) medium and the mineral composition considered adequate in the literature. This approach suggested that calcium (Ca) is a critical nutrient and this was considered for new media formulation. Three new culture media were defined in which the main changes to half-strength MS medium were an increase in Ca, magnesium, sulfur, copper, and chloride and a decrease in iron, maintaining the nitrate:ammonium rate at ≈2:1. The main difference among the three new media formulated was Ca concentration, which varied from 1.5 mm in half-strength MS to 3.0, 6.0, and 12 mm in M2, M3, and M4 media, respectively. Consistently, all three species exhibited significantly higher fresh and dry weight on M4, the newly defined medium with the highest level of Ca (12 mm). Leaf nitrogen, potassium, zinc, magnesium, and boron concentrations increased as Ca concentration in the medium increased from 1.5 to 12 mm.

scholarly journals Combining Medicinal Plant In Vitro Culture with Machine Learning Technologies for Maximizing the Production of Phenolic Compounds

Antioxidants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 210 ◽  
Author(s):  
Pascual García-Pérez ◽  
Eva Lozano-Milo ◽  
Mariana Landín ◽  
Pedro Pablo Gallego
Keyword(s):  
Machine Learning ◽  
Phenolic Compounds ◽  
In Vitro Culture ◽  
Culture Media ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Starting Point ◽  
Plant In Vitro Culture

We combined machine learning and plant in vitro culture methodologies as a novel approach for unraveling the phytochemical potential of unexploited medicinal plants. In order to induce phenolic compound biosynthesis, the in vitro culture of three different species of Bryophyllum under nutritional stress was established. To optimize phenolic extraction, four solvents with different MeOH proportions were used, and total phenolic content (TPC), flavonoid content (FC) and radical-scavenging activity (RSA) were determined. All results were subjected to data modeling with the application of artificial neural networks to provide insight into the significant factors that influence such multifactorial processes. Our findings suggest that aerial parts accumulate a higher proportion of phenolic compounds and flavonoids in comparison to roots. TPC was increased under ammonium concentrations below 15 mM, and their extraction was maximum when using solvents with intermediate methanol proportions (55–85%). The same behavior was reported for RSA, and, conversely, FC was independent of culture media composition, and their extraction was enhanced using solvents with high methanol proportions (>85%). These findings confer a wide perspective about the relationship between abiotic stress and secondary metabolism and could serve as the starting point for the optimization of bioactive compound production at a biotechnological scale.

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