scholarly journals Performance of different protocols on in vitro tuberization in potato (Solanum tuberosum)

2014 ◽  
Vol 39 (1) ◽  
pp. 59-66 ◽  
Author(s):  
M Zakaria ◽  
MM Hossain ◽  
MA Khaleque Mian ◽  
T Hossain
Keyword(s):  
Solanum Tuberosum ◽  
Growth Retardant ◽  
Average Weight ◽  
Dark Condition ◽  
In Vitro Tuberization ◽  
Potato Varieties ◽  
Large Size

Five protocols of micro tuberization were used to induce large size microtuber in three recommended potato varieties, namely Cardinal, Diamant, and Heera under complete dark condition. Tuberization was the earliest (11.8 days) in the protocol P2 (MS + 5 mg/l BAP + 500 mg/l CCC + 8% sucrose), which was closely followed by that in P1 (12.7 days) (MS + 5 mg/l BAP + 50 mg/l coumarin + 8% sucrose). Maximum number of microtubers/flask (12.8) was obtained from the protocol P1 followed by that of P2 (11.6) that contained growth retardant; but higher average weight of microtuber was obtained in the protocols P5 (30 days old plantlet + MS media containing 40 meq K + 10 mg/l BA + 9% sucrose), P4 (MS + 10 mg/l BA + 8% sucrose), and P3 (MS + 5.0 mg/l BAP + 6% sucrose) which contained BA in absence of growth retardant. The average weight of microtuber was the highest (329.0 mg) in protocol P5, followed by that in P4 (280.7 mg), while it was the lowest in protocol P1. The variety Diamant produced maximum average weight of microtuber (246.3 mg), while Heera produced minimum (226.1 mg), which was statistically similar to Cardinal (228.7 mg). The highest percentage (52.2) of >300 mg size and lowest percentage (19.3) of <150 mg size microtuber was produced in P5 protocol in the variety Diamant. On overall consideration, all the varieties performed best with the protocol P5. DOI: http://dx.doi.org/10.3329/bjar.v39i1.20143 Bangladesh J. Agril. Res. 39(1): 59-66, March 2014

scholarly journals Effect of Nitrogen and Potassium on In vitro Tuberization of Potato

1970 ◽  
Vol 17 (1) ◽  
pp. 79-85 ◽  
Author(s):  
M Zakaria ◽  
MM Hossain ◽  
MA Khaleque Mian ◽  
T Hossain ◽  
N Sultana
Keyword(s):  
Total Nitrogen ◽  
Optimum Concentration ◽  
Nitrogen Level ◽  
Average Weight ◽  
Ms Medium ◽  
Standard Protocol ◽  
In Vitro Tuberization

Effects of nitrogen @ 20, 40, 60 and 80 meq in combination with potassium @ 10, 20, 30, 40 and 50 meq on in vitro tuberization of potato were evaluated to find out the optimum concentration of nitrogen and potassium for developing a standard protocol for in vitro tuberization of a popular variety Diamant. Microtuberization was delayed with increasing rate of nitrogen. Number of microtubers were reduced with an increase in total nitrogen but the average weight of microtubers increased with an increase in nitrogen level up to 60 meq. The number and size of microtubers increased with an increase in potassium up to 40 meq. Concentration of nitrogen at 60 meq and potassium at 40 meq in MS medium gave rise to microtubers of large sizeKey words: In vitro, Tuberization, PotatoDOI = 10.3329/ptcb.v17i1.1124Plant Tissue Cult. & Biotech. 17(1): 79-85, 2007 (June)

The Study of Rhizogenes of Potato Varieties (Solanum tuberosum L.) in vitro

2013 ◽  
pp. 69-72 ◽  
Author(s):  
Ksenia Guseva ◽  
Irina Borodulina ◽  
Elena Myakisheva ◽  
Otari Tavartkiladze
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Potato Varieties

Effect of photoperiod on in vitro tuberization of potato (Solanum tuberosum L.)

1993 ◽  
Vol 34 (1) ◽  
pp. 43-51 ◽  
Author(s):  
Janet E. A. Seabrook ◽  
Shirlyn Coleman ◽  
David Levy
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
In Vitro Tuberization

EFFECTS OF CULTURE DENSITY ON GROWTH AND IN VITRO TUBERIZATION CAPACITY OF POTATO PLANTLETS

2000 ◽  
Vol 48 (2) ◽  
pp. 185-189
Author(s):  
K. Tábori ◽  
A. Ferenczy ◽  
J. Dobránszki
Keyword(s):  
Solanum Tuberosum ◽  
Plant Density ◽  
Solanum Tuberosum L ◽  
Stem Length ◽  
Tuber Initiation ◽  
In Vitro Tuberization ◽  
In Vitro Plantlets ◽  
Plant Densities ◽  
Potato Plantlets

In vitro plantlets of four varieties of potato (Solanum tuberosum L.) were grown at different plant densities to study their effects on growth and microtuber yield. The responses of the cultivars to plant density, as expressed by changes in development characteristics, especially stem length and leaf area, were different. The time of tuber initiation was earlier at higher plantlet densities for all cultivars. The microtuber number per plantlet was not affected by the plantlet density. The highest number of well-sized (≯4 mm) microtubers per jar could be harvested from the higher plantlet densities (30–40 plantlets per jar). In addition to good yield, the microtubers were uniform in these treatments, so they appear to be economical.

Jasmonate effect on in vitro tuberization of potato (Solanum tuberosum L.) cultivars under light and dark conditions

2001 ◽  
Vol 44 (4) ◽  
pp. 315-325 ◽  
Author(s):  
K. Pruski ◽  
P. Duplessis ◽  
T. Lewis ◽  
T. Astatkie ◽  
J. Nowak ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
In Vitro Tuberization ◽  
Dark Conditions

In vitro tuberization of the common potato (Solanum tuberosum) is not a response to the osmotic concentration of the medium

1972 ◽  
Vol 50 (3) ◽  
pp. 603-605 ◽  
Author(s):  
Fung Mei Lo ◽  
B. R. Irvine ◽  
W. G. Barker
Keyword(s):  
Solanum Tuberosum ◽  
Energy Requirements ◽  
In Vitro Tuberization ◽  
Osmotic Concentration ◽  
Common Potato ◽  
The Common

It has been speculated that the rapid tuberization of explanted etiolated stolon nodes of the common potato relates to the osmotic concentration of the medium since this response is speeded by increased sucrose levels up to 12%. This paper demonstrates that the response probably pertains to the energy requirements of the tuberizing explanted stolon and that osmotic concentration is not crucial.

scholarly journals EFFECT OF SILVER THIOSULPHATE AND PHOTOPERIOD ON IN VITRO TUBERIZATION OF THREE POTATO (SOLANUM TUBEROSUM L.) CULTIVARS

2021 ◽  
Vol 21 (Suppliment-1) ◽  
pp. 308-317
Author(s):  
S.F. El-Sayed ◽  
Sahar S. Taha ◽  
Omaima S. Darwish ◽  
Saumu Z. Mwessongo
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
In Vitro Tuberization ◽  
Silver Thiosulphate

scholarly journals The Effects of Different Concentrations of Gibberellic Acid (GA3) and Kinetin on in Vitro Tuberization of Potato (Solanum tuberosum L.)

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 752C-752
Author(s):  
Servet Kefi ◽  
Paul E. Read ◽  
Alexander Pavlista ◽  
Stephen D. Kachman
Keyword(s):  
Solanum Tuberosum ◽  
Gibberellic Acid ◽  
Solanum Tuberosum L ◽  
Short Photoperiod ◽  
In Vitro Tuberization ◽  
High Concentration ◽  
In Vitro Plantlets ◽  
Murashige And Skoog Medium ◽  
Cultivar Atlantic

To determine the influence of gibberellic acid (GA3) and 6-furfuryl aminopurine (kinetin) concentrations alone and in combinations on in vitro tuberization of potato, nine treatments consisting of combinations of gibberellic acid and kinetin at three levels of concentration (0, 2, and 5 mg·liter–1) were included in Murashige and Skoog medium supplemented with 6% sucrose. Four single nodes of in vitro plantlets from Solanum tuberosum L. cultivar Atlantic were placed into each magenta box. All magenta boxes were arranged in a randomized complete box design with five replications and cultured under a short photoperiod condition (8 h light/16 h dark). Gibberellic acid strongly inhibited tuberization when used alone or with kinetin, whereas kinetin induced tuberization at both 2 and 5 mg·liter–1. Although tuberization was initiated in the absence of kinetin because of the high concentration of sucrose and short photoperiod, the presence of kinetin accelerated the in vitro tuberization process of potato.

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