In vitro tuberization in potato varieties of different ripe time

2021 ◽  
Vol 22 (Special) ◽  
Keyword(s):  
In Vitro Tuberization ◽  
Potato Varieties

scholarly journals Performance of different protocols on in vitro tuberization in potato (Solanum tuberosum)

2014 ◽  
Vol 39 (1) ◽  
pp. 59-66 ◽  
Author(s):  
M Zakaria ◽  
MM Hossain ◽  
MA Khaleque Mian ◽  
T Hossain
Keyword(s):  
Solanum Tuberosum ◽  
Growth Retardant ◽  
Average Weight ◽  
Dark Condition ◽  
In Vitro Tuberization ◽  
Potato Varieties ◽  
Large Size

Five protocols of micro tuberization were used to induce large size microtuber in three recommended potato varieties, namely Cardinal, Diamant, and Heera under complete dark condition. Tuberization was the earliest (11.8 days) in the protocol P2 (MS + 5 mg/l BAP + 500 mg/l CCC + 8% sucrose), which was closely followed by that in P1 (12.7 days) (MS + 5 mg/l BAP + 50 mg/l coumarin + 8% sucrose). Maximum number of microtubers/flask (12.8) was obtained from the protocol P1 followed by that of P2 (11.6) that contained growth retardant; but higher average weight of microtuber was obtained in the protocols P5 (30 days old plantlet + MS media containing 40 meq K + 10 mg/l BA + 9% sucrose), P4 (MS + 10 mg/l BA + 8% sucrose), and P3 (MS + 5.0 mg/l BAP + 6% sucrose) which contained BA in absence of growth retardant. The average weight of microtuber was the highest (329.0 mg) in protocol P5, followed by that in P4 (280.7 mg), while it was the lowest in protocol P1. The variety Diamant produced maximum average weight of microtuber (246.3 mg), while Heera produced minimum (226.1 mg), which was statistically similar to Cardinal (228.7 mg). The highest percentage (52.2) of >300 mg size and lowest percentage (19.3) of <150 mg size microtuber was produced in P5 protocol in the variety Diamant. On overall consideration, all the varieties performed best with the protocol P5. DOI: http://dx.doi.org/10.3329/bjar.v39i1.20143 Bangladesh J. Agril. Res. 39(1): 59-66, March 2014

Identification of Differentially Expressed Genes Using cDNA-AFLP During Six Days In Vitro Tuberization of Potato

Zuriat ◽  
2015 ◽  
Vol 14 (1) ◽  
Author(s):  
Nono Carsono ◽  
Christian Bachem
Keyword(s):  
Gene Expression ◽  
Developmental Process ◽  
Expression Patterns ◽  
Induction Medium ◽  
In Vitro Tuberization ◽  
Storage Organ ◽  
Developmentally Regulated ◽  
Study Gene Expression ◽  
Differential Pattern

Tuberization in potato is a complex developmental process resulting in the differentiation of stolon into the storage organ, tuber. During tuberization, change in gene expression has been known to occur. To study gene expression during tuberization over the time, in vitro tuberization system provides a suitable tool, due to its synchronous in tuber formation. An early six days axillary bud growing on tuber induction medium is a crucial development since a large number of genes change in their expression patterns during this period. In order to identify, isolate and sequencing the genes which displaying differential pattern between tuberizing and non-tuberizing potato explants during six days in vitro tuberization, cDNA-AFLP fingerprint, method for the visualization of gene expression using cDNA as template which is amplified to generate an RNA-fingerprinting, was used in this experiment. Seventeen primer combinations were chosen based on their expression profile from cDNA-AFLP fingerprint. Forty five TDFs (transcript derived fragment), which displayed differential expressions, were obtained. Tuberizing explants had much more TDFs, which developmentally regulated, than those from non tuberizing explants. Seven TDFs were isolated, cloned and then sequenced. One TDF did not find similarity in the current databases. The nucleotide sequence of TDF F showed best similarity to invertase ezymes from the databases. The homology of six TDFs with known sequences is discussed in this paper.

Interaction between jasmonic and gibberellic acids on in vitro tuberization of potato plantlets

2000 ◽  
Vol 43 (1) ◽  
pp. 83-88 ◽  
Author(s):  
Gabriela Castro ◽  
Guillermina Abdala ◽  
Cecilia Agüero ◽  
R. Tizio
Keyword(s):  
In Vitro Tuberization ◽  
Potato Plantlets

scholarly journals How to produce large sized microtubers of potato cv. Desiree

2002 ◽  
Vol 8 (3-4) ◽  
Author(s):  
K. Magyar-Tábori ◽  
J. Dobránszky
Keyword(s):  
Size Distribution ◽  
Multiplication Rate ◽  
In Vitro Tuberization ◽  
Fresh Weight ◽  
Microtuber Production ◽  
High Sucrose ◽  
Very High

In vitro tuberization was induced on explants with different number of nodes layered on a medium with high sucrose (8%) content: 30, 15, 10, 7 and 6 explants per jar were cultured containing 1, 2, 3, 4 or 5 nodes, respectively. Microtubers developed were graded by their smallest diameter, and the number of tubers per jar, their size distribution, their fresh weight and the multiplication rate were recorded. The highest multiplication rate (1.98) was obtained for explants with 5 nodes. The size distribution of tubers was markedly affected by treatments. The majority of microtubers (49.4%) were 6-8 mm in the case of the smallest explants (with I node). When explants with 2 to 5 nodes were used, the most microtubers were 8-10 mm but with an increase of explant size, more and more microtubers were produced with larger diameter up to 16 mm and average fresh weight of tubers also increased with the increase of explant size. For the microtuber production of Desiree the use of explants with two nodes can be suggested because in this treatment the average fresh weight of microtubers was high enough (250 mg) and the number of large sized microtubers was very high (79% was larger than 6 mm and 53% was larger than 8 mm).

scholarly journals IMPROVEMENT OF POTATO SEED MATERIAL AND ITS DIAGNOSTICS IN SYSTEM OF BIOTECHNOLOGY

2012 ◽  
Vol 14 ◽  
pp. 156-166
Author(s):  
T.M. Oleynik ◽  
K.A. Sloblodyan ◽  
S.A. Slobodyan ◽  
R.V. Gricay
Keyword(s):  
Molecular Diagnosis ◽  
Potato Virus ◽  
Antiviral Drugs ◽  
Field Conditions ◽  
Potato Seed ◽  
Potato Varieties ◽  
In Vitro Plants ◽  
Seed Material ◽  
Potato Virus M

The results of improvement studies of potato varieties by chemotherapy along with the use of antiviral drugs: RNA-ase, acyclovir, izatizon, and hydrochloride as well as data on the molecular diagnosis of X-and M-viruses in vitro plants, resulting from the recovery are presented. 3 lines free from virus X and 4 lines free from potato virus M were allocated. After the testing of variety changeability and its economically valuable characteristics in field conditions one of them will be selected and submitted to the Bank in vitro redeveloped varieties.

scholarly journals Variability in salinity stress tolerance of potato (Solanum tuberosum L.) varieties using in vitro screening

2020 ◽  
Vol 44 ◽  
Author(s):  
Hussein Abdullah Ahmed Ahmed ◽  
Nilüfer Koçak Şahin ◽  
Güray Akdoğan ◽  
Cennet Yaman ◽  
Deniz Köm ◽  
...  
Keyword(s):  
Salt Stress ◽  
Osmotic Stress ◽  
Stress Tolerance ◽  
Salinity Stress ◽  
Morphological Characteristics ◽  
In Vitro Screening ◽  
Salt Tolerant ◽  
Potato Varieties ◽  
Salinity Stress Tolerance

ABSTRACT Salinity is one of the abiotic stresses that lead to an imbalance in the physiological processes of the plants and also affects potato growth and productivity in maınly semi-arid and growing areas. The accumulation of Na+ and Cl- ions in the cells is very toxic can influence all mechanisms and the enzymatic actions of the plants. In vitro screening of plant genotypes for osmotic stress represents a valuable tool as an alternative to field trials and can be applied based on osmotic stress tolerance. The main goal of this study was to reveal variability in salinity stress tolerance of potato varieties using in vitro screening. Stem cuttings consisting of a single node of different varieties were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of sodium chloride (NaCl) (0, 50, 100 and 150 mM). The differences among the plantlet length, number of branches, number of nodes, number of the leaflet, leaflet width, leaflet length, root length, number of the root, fresh plantlet weight, dry plantlet weight of all varieties were negatively influenced by all NaCl concentrations tested. Microtuberization and stolon growth of the varieties were also completely inhibited at high concentrations (100-150 mM). The Principal components analysis (PCA) was applied to the data matrix (15 morphological characteristics x 12 potato varieties) of the potato varieties. Also, a hierarchical cluster analysis (HCA) was used to identify the possible nearest and similarity of all morphological characteristics analyzed of the potato varieties. In grouping potato varieties, HCA and PCA results were found to be similar. We can speculate about the responses of morphological similarities of the potato varieties against salt stress. We concluded that Innovator and Kennebec are respectively the most salt-tolerant varieties. Hermes was moderately salt-tolerant and microtuberization capacity of Slaney was also high under salt stress conditions.

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