scholarly journals Genetic diversity analysis of lactic acid bacteria isolated from regional yogurt samples

2021 ◽  
pp. 55-63
Author(s):  
KM Hossain ◽  
B Mazumder ◽  
SMM Rahman ◽  
MA Hamid
Keyword(s):  
Genetic Diversity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Genetic Distance ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Probiotic Bacteria ◽  
Diversity Analysis ◽  
Biochemical Tests ◽  
Genetic Diversity Analysis

Probiotics are live, non-pathogenic microorganisms which have enormous health benefits. Yogurt is a potential source of probiotic bacteria. Lactic acid bacteria are the most common and major group of probiotic bacteria that can be found easily in different dairy products. The aim of study was the genetic diversity analysis using random amplified polymorphic DNA (RAPD) marker from regional yogurt samples of Bangladesh. Ten probiotic isolates from yogurt samples of different sweet meat shops of Sylhet and Mymensingh Divisions of Bangladesh were used. Morphological and biochemical tests were performed to ensure the presumptive probiotic characteristics of isolated bacteria. All isolates were cultured in De Man, Rogosa, and Sharpe (MRS) medium to exhort the growth of lactic acid bacteria. Genomic DNA was extracted by Ampicillin Lysozyme Tandem method. Four random RAPD primers were used in this study for detecting genetic diversity of these isolated bacteria. Among them, OPA 18 showed the maximum number of reproducible bands. Nei’s genetic distance was performed for determining Pair-wise Genetic Distance. UPGMA and NJ dendrogram were performed based on molecular data showing that all the isolates could be divided into two major clusters. Data analysis revealed that isolates from the same location were closely related and showed less genetic variation whereas, isolates from geographically different regions exhibited more genetic diversity. Bang. J. Livs. Res. Vol. 27 (1&2), 2020: P. 55-63

scholarly journals Pemilihan Tetua Persilangan pada Kubis (Brassica oleracea var. capitata) melalui Analisis Keragaman Genetik [Parental Line Selection in Cabbage (Brassica oleracea var. capitata) through Genetic Diversity Analysis]

2019 ◽  
Vol 28 (1) ◽  
pp. 33
Author(s):  
Nur Kholilatul Izzah ◽  
NFN Reflinur
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Brassica Oleracea ◽  
Diversity Analysis ◽  
Heterotic Groups ◽  
Genetic Diversity Analysis ◽  
National University ◽  
Parental Lines ◽  
Seoul National University ◽  
Simple Sequence

<p>Kubis (<em>Brassica oleracea</em> var. <em>capitata</em>) merupakan salah satu jenis sayuran yang mempunyai nilai ekonomis tinggi. Untuk meningkatkan hasil panen kubis tiap tahunnya perlu didukung oleh tersedianya varietas unggul yang tahan penyakit, terutama penyakit busuk hitam dan akar gada yang dapat menggagalkan panen. Metode yang dapat diaplikasikan untuk merakit varietas unggul adalah melalui persilangan. Penelitian ini bertujuan untuk memilih kombinasi tetua persilangan yang ideal pada tanaman kubis melalui analisis keragaman genetik menggunakan marka SSR (<em>Simple Sequence Repeats</em>). Penelitian dilakukan pada bulan Februari sampai Mei 2013 di laboratorium Functional Crop Genomics and Biotechnology, Seoul National University, Korea Selatan menggunakan 16 genotipe kubis yang diperoleh dari perusahaan benih Joeun, Korea Selatan. Keragaman genetik 16 genotipe kubis dianalisis menggunakan 35 marka SSR polimorfik, dan selanjutnya digunakan untuk menentukan keragaman genetik berdasarkan metode UPGMA. Nilai jarak genetik antar genotipe diperoleh berdasarkan rumus 1-nilai kesamaan genetik. Hasil analisis keragaman genetik membagi 16 genotipe kubis menjadi dua kelompok heterotik utama pada nilai kesamaan genetik 65,2%. Berdasarkan hasil analisis keragaman genetik dan nilai jarak genetik diperoleh empat kombinasi tetua persilangan ideal, yaitu genotipe IMO-03 vs IMO-08 (nilai jarak genetik 43%) dan IMO-03 vs IMO-10 (nilai jarak genetik 39%) untuk karakter ketahanan terhadap penyakit busuk hitam, serta genotipe IMO-18 vs IMO-10 dan IMO-17 vs IMO-10 dengan nilai jarak genetik masing-masing 45% dan 44% untuk karakter ketahanan terhadap penyakit akar gada. Keempat kombinasi tetua tersebut dipilih karena terletak pada kelompok heterotik berbeda serta mempunyai nilai jarak genetik yang jauh sehingga diharapkan dapat meningkatkan peluang heterosis pada progeni yang dihasilkan.</p><p><strong>Keywords</strong></p><p><em>Brassica oleracea</em> var. <em>capitata</em>; Genotipe; Keragaman genetik; Kubis; Pemilihan tetua</p><p><strong>Abstract</strong></p><p>Cabbage (<em>Brassica oleracea</em> var. <em>capitata</em>) is one of vegetable that has high economic value. The availability of high-yielding varieties that are resistant to some diseases, particularly black rot and clubroot disease is needed in order to increase cabbage yield per year. The method which can be applied to assemble new varieties is through crossbreeding program. The objective of this research was to select the ideal combination of parental lines in cabbage through analysis of genetic diversity by using SSR markers (Simple Sequence Repeats). The research was conducted from February to May 2013 in the laboratory of Crop Functional Genomics and Biotechnology, Seoul National University, South Korea using 16 cabbage genotypes obtained from Joeun seed company, South Korea. The genetic diversity of 16 cabbage genotypes were analyzed using 35 polymorphic SSR markers, and then used to determine the genetic diversity based on UPGMA method. Meanwhile, genetic distance value among cabbage genotypes obtained by the formula of 1-value genetic similarity. The results of genetic diversity analysis divided 16 cabbage genotypes into two main heterotic groups at genetic similarity value of 65.2%. Based on the results of genetic diversity analysis and genetic distance value, we selected four combinations of ideal parental lines, namely genotype IMO-03 vs IMO-08 (genetic distance value of 43%) and IMO-03 vs IMO-10 (genetic distance value of 39%) for black rot disease resistance character, as well as genotype IMO-18 vs IMO-10 and IMO-17 vs IMO-10 with genetic distance value of 45% and 44%, respectively for club root disease resistance character. These four parental lines combination were selected as ideal parental combinations due to they were located on a different heterotic groups and has high genetic distance value, which is expected to increase the chances of heterosis in their progeny.</p>

scholarly journals Genetic Diversity Analysis of Couroupita guianensis Aubl.based on RAPD Marker

2017 ◽  
Vol 8 ◽  
Author(s):  
Santosh P ◽  
Suresh B. Arakera
Keyword(s):  
Genetic Diversity ◽  
Rapd Marker ◽  
Habitat Destruction ◽  
Diversity Analysis ◽  
Native Range ◽  
Human Settlement ◽  
Genetic Diversity Analysis ◽  
Couroupita Guianensis ◽  
Rapd Pcr ◽  
Native Populations

<p> <strong><em>Couroupita guianensis</em> Aubl. Commonly known as cannon ball tree, belongs to family Lecythidaceae and widely used in Chinese medicine. C. guianensis is being threatened due to habitat destruction in its native range for human settlement and other development related activities. Native populations of C. guianensis  were collected from different locations of  the India states and characterized through. To study the genetic diversity of C. guianensis  populations we performed RAPD – PCR with several primers. The results are revealed in this study.</strong></p>

scholarly journals Genetic diversity analysis of thirteen mungbean (Vigna radiata (L.) Wilczek) cultivars using RAPD markers

2013 ◽  
Vol 41 (2) ◽  
pp. 169-175 ◽  
Author(s):  
Sonia Khan Sony ◽  
Md Ahashan Habib ◽  
Mohammad Nurul Islam
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Genetic Distances ◽  
Polymorphic Band ◽  
Group Method ◽  
Diversity Analysis ◽  
Arithmetic Means ◽  
Genetic Diversity Analysis ◽  
Pair Group ◽  
Band Size

Genetic diversity analysis among 13 mungbean cultivars from Bangladesh was performed through polymerase chain reaction (PCR) based random amplification of polymorphic DNA (RAPD). Out of 20 arbitrary decamer oligonucleotide primers used, 10 produced a total of 379 different bands with an average of 37.9 bands per primer. Based on the observed banding pattern all the primers were found to be 100% polymorphic. Band size of the amplicons ranged from 250 - 5000 bp. A total of 10 unique DNA fragments was amplified from the 13 mungbean cultivars genome. The values of pair-wise genetic distances ranged from 0.0700 - 1.0852, indicating the presence of wide genetic diversity. The highest genetic distance (1.0852) was found between cultivar BARI Mung-2 and 6 while the lowest (0.0700) between cultivar BINA Mung-2 and 7. Dendogram based on Nei’s genetic distance using Unweighted Pair Group Method of Arithmetic Means (UPGMA) has segregated the 13 mungbean cultivars into two major clusters. BARI Mung-1, 2, 3, 4 and 5 formed cluster 1 and BARI Mung-6, BINA Mung-1, 2, 7, 6, 4, 5 and 8 have made cluster 2. DOI: http://dx.doi.org/10.3329/bjb.v41i2.13444 Bangladesh J. Bot. 41(2): 169-175, 2012 (December)

Sign in / Sign up

Export Citation Format

Share Document