scholarly journals GENETIC DIVERSITY OF BALAM AND JESSO-BALAM RICE (Oryza sativa L.) GERMPLASM OF BANGLADESH REVEALED BY SSR MARKERS

2016 ◽  
Vol 27 (2) ◽  
pp. 17-24
Author(s):  
M. S. Ahmed ◽  
M. Khalequzzaman ◽  
M. K. Bashar ◽  
A. K. M. Shamsuddin
Keyword(s):  
Genetic Diversity ◽  
Oryza Sativa ◽  
Genetic Distance ◽  
Ssr Markers ◽  
Oryza Sativa L ◽  
Gene Diversity ◽  
Clustering Method ◽  
Land Races ◽  
Rice Genotypes

A total of 72 rice genotypes including Balam (40), Jesso-Balam (27) rice land races and popular varieties (5) from Bangladesh were characterized using 45 SSR markers for studying genetic diversity and identification of duplicate germplasm. Altogether 430 alleles were detected which varied from 4 to 18 per locus with an average of 9.6. The highest number of alleles (18) was found in the locus RM302. The highest gene diversity (0.91) was found in RM337 and RM224, while the lowest (0.52) was found in RM237. The PIC values ranged from 0.90 to 0.46 with an average of 0.78. The highest PIC value was observed in loci RM337 and RM224 followed by 0.89 in RM55, RM258, RM21 and RM206, respectively. The gene diversity and PIC values revealed that RM337, RM224, RM55, RM258, RM21 and RM206 were the best markers to identify and distinguish the genotypes. Besides, the UPGMA clustering method generated seven clusters, where no duplicate genotype was found. It also showed that Balam and Jesso-Balam groups of germplasm were constellated into separate clusters. The Nei’s genetic distance ranged from 0.3556 to 1.0. Conserved, characterization and utilization of the unique and distinct variability of all the similar or duplicate named land races of Balam and Jesso-Balam rice is suggested.

scholarly journals Genetic Diversity in Aus rice (Oryza sativa L.) Genotypes of Bangladesh

2018 ◽  
Vol 43 (2) ◽  
pp. 253-266
Author(s):  
N Akter ◽  
H Begum ◽  
MZ Islam ◽  
MA Siddique ◽  
M Khalequzzaman
Keyword(s):  
Genetic Diversity ◽  
Oryza Sativa ◽  
Genetic Distance ◽  
Oryza Sativa L ◽  
Mean Value ◽  
Tiller Number ◽  
Cluster Distance ◽  
Transgressive Segregants ◽  
Rice Genotypes ◽  
High Genetic Distance

Genetic diversity in 31 traditional Bangladeshi Aus rice genotypes were studied under transplanted condition through Mahalanobis D2 statistic for grain yield and yield contributing characters. The genotypes were grouped into five clusters. The inter-cluster distances were higher than intra-cluster distances indicating wider genetic diversity among the genotypes of different clusters. The intra-cluster distances were lower in all the cases reflecting homogeneity of the genotypes within the clusters. The cluster II contained the highest number of genotypes (08) and the cluster I and III contained the lowest (05). The highest intra-cluster distance was noticed for the cluster II and the lowest for cluster IV. The highest inter-cluster distance was observed between cluster II and V followed by cluster III and V, cluster I and V and the lowest between cluster I and III. Regarding inter-cluster distance, the genotypes of cluster V showed high genetic distance from all other clusters. The genotypes from cluster V could be hybridized with the genotypes of other characters of other clusters for producing transgressive segregants. The highest cluster means for yield, effective tiller number and grain length, were obtained from cluster I; whereas the lowest mean value for yield, culm length, plant height and grain breadth were found in cluster II. Therefore, genotypes under cluster I, cluster II and cluster V might be selected for future breeding program as parents for crossing to produce new recombinants with desired traits.Bangladesh J. Agril. Res. 43(2): 253-266, June 2018

scholarly journals ANALISIS KERAGAMAN GENETIK PLASMA NUTFAH KAKAO (Theobroma cacaoL.) BERDASARKANMARKA SSR / Analysis of Genetic Variability Germplasm of Cacao (Theobroma cacaoL.)Basedon SSR Marker

2020 ◽  
Vol 17 (4) ◽  
pp. 156
Author(s):  
Surti Kurniasih ◽  
Rubiyo Rubiyo ◽  
Asep Setiawan ◽  
Agus Purwantara ◽  
Sudarsono Sudarsono
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Ssr Markers ◽  
Theobroma Cacao ◽  
Simple Sequence Repeat ◽  
Ssr Marker ◽  
Gene Diversity ◽  
Sequence Repeat ◽  
Theobroma Cacao L ◽  
Simple Sequence

<p>Microsatellite or simple sequence repeat (SSR) markers have proven to be an excellent tool for cultivar identification, pedigree analysis, and genetic distance evaluations among organisms. The objectives of this research were to characterize cacao collection of Indonesian Coffee and Cacao Research Institute (ICCRI) and to analyze their genetic diversity using SSR markers. In this research, 39 SSR primer pairs were used to amplify genomic DNA of 29 cacao clones. Amplified SSR fragments for each primer pair were scored as individual band and used to determine genetic distance among evaluated cacao clones. Results of the experiment indicated that all SSR primer pairs evaluated were able to produce SSR markers for 29 cacao clones. The results also indicated that 34 out of 39 microsatellite loci evaluated were polymorphic, while 5 others were monomorphic. The total number of observed alleles among 29 clones was 132. Number of alleles per locus ranged from 4-8, with an average of 5.5 alelles per locus. Results of data analysis indicated that the PIC value was 0.665, the observed heterozigosity (Ho) was 0.651, and the gene diversity (He) was 0.720. The PIC, Ho, and He values were considered high. Genetic distances were evaluated using NTSys version 2.1 and dendrogram was constructed. Results of analysis indicated that 12 cacao clones evaluated were clustered in the first group with diversity coefficient of &lt; 3.75. Nine cacao clones were in the second group but with the same value of diversity coefficient (&lt;7.50). The rest of the cacao clones were in the third group with diversity coefficient of&gt;7.50. Based on those finding, all SSR primer pairs evaluated could be used to analyze cacao genome and be useful for genetic diversity analysis of cacao germplasm. The SSR marker analysis in ICCRI cacao collections resulted in high PIC, high observed heterozygosity, and high genetic diversity.</p><p>Key words: Theobroma cacao L, microsatelite, molecular marker, genetic diversity, heterozygosity</p><p> </p><p><strong>Abstrak</strong></p><p>Marka mikrosatelit atau sekuens sederhana berulang (simple sequence repeat = SSR) terbukti merupakan alat yang bagus untuk identifikasi kultivar, analisis pedigree, dan evaluasi jarak genetik berbagai organisme. Penelitian ini bertujuan untuk:1) karakterisasi kakao koleksi Pusat penelitian Kopi dan Kakao Indonesia menggunakan marka SSR dan 2) analisis keragaman genetik klon-klon kakao koleksi dengan menggunakan marka SSR. Dalam penelitian ini, 39 pasangan primer SSR telah digunakan untuk amplifikasi DNA genomik dari 29 klon kakao. Skoring pita SSR hasil amplifikasi menggunakan masing-masing pasangan primer dilakukan secara terpisah dan digunakan untuk menentukan jarak genetik di antara klon kakao yang dievaluasi. Hasil percobaan menunjukkan bahwa semua pasangan primer SSR yang digunakan mampu menghasilkan pita DNA hasil amplifikasi (marka SSR) untuk 29 klon kakao yang diuji. Hasil penelitian juga menunjukkan bahwa 34 dari 39 lokus SSR yang dianalisis bersifat polimorfik sedangkan lima primer yang lain bersifat monomorfik. Dari 29 klon kakao yang dievaluasi, telah berhasil diamplifikasi sebanyak 132 alel, dengan kisaran antara 4-8 alel/lokus. Rataan jumlah alel per lokus sebanyak 5,50. Hasil analisis data yang dilakukan juga menunjukkan nilai PIC untuk marka SSR yang digunakan sebesar 0,665. Untuk populasi klon kakao yang dievaluasi, diperoleh nilai rataan heterosigositas pengamatan (Ho) sebesar 0,651 dan rataan diversitas gen (He) sebesar 0,720. Nilai PIC Ho dan He yang didapat tergolong tinggi. Berdasarkan analisis keragaman dengan menggunakan program NTSys, diperoleh hasil 12 klon kakao berada dalam grup pertama (koefisien keragaman&lt;3,75) dan9 klon berada dalam grup kedua, dengan koefisien keragaman &lt; 7,50. Sedangkan klon-klon lainnya mempunyai koefisien keragaman &gt; 7,50. Berdasarkan hasil penelitian dan analisis data disimpulkan bahwa marka SSR dapat digunakan untuk menganalisis keragaman genetik plasma nutfah kakao. Tingkat polimorfisme yang dihasilkan marka SSR relatif tinggi. Tingkat heterosigositas plasma nutfah kakao koleksi Puslit Kopi dan Kakao Indonesiarelatif tinggi, dan keragaman genetiknyacukup tinggi.</p><p>Kata kunci : Theobroma cacao L, mikrosatelit, marka molekuler, keragaman genetik, heterosigositas</p>

Genetic diversity for submergence tolerance in rice (Oryza sativa L.) Utilizing SSR Markers

2019 ◽  
Vol 21 (2) ◽  
pp. 151
Author(s):  
Nilmani Prakash ◽  
Yaswant Kumar Pankaj ◽  
Rajesh Kumar ◽  
Pankaj Kumar ◽  
Raj Kumar Jat
Keyword(s):  
Genetic Diversity ◽  
Oryza Sativa ◽  
Ssr Markers ◽  
Oryza Sativa L ◽  
Submergence Tolerance

scholarly journals Genetic diversity assessment of Rice (Oryza sativa L.) germplasm using ssr markers

2015 ◽  
Vol 1 (1) ◽  
pp. 37-46 ◽  
Author(s):  
Ahasanul Hoque ◽  
Shamsun Nahar Begum ◽  
Lutful Hassan
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Oryza Sativa L ◽  
Growth Duration ◽  
Breeding Programs ◽  
Diversity Assessment ◽  
Days To Heading ◽  
Early Maturing ◽  
Rice Genotypes ◽  
Selection Of

Diversity at molecular level among thirty rice genotypes, selected based on earliness and morphometric diversity was evaluated through five SSR markers associated with days to heading. Three primers viz., RM147, RM167 and RM215 showed polymorphism for growth duration related traits. A total of 17 alleles were detected among the 30 rice genotypes with an average of 5.66 alleles per locus. Polymorphism Information Content (PIC) ranged from 0.356 to 0.798 with an average of 0.543. A dendrogram based on total microsatellite polymorphism grouped 30 genotypes into four major clusters at 0.39 similarity coefficient differentiating early maturing genotypes from others. This information about the genetic diversity will be very useful for proper identification and selection of appropriate parents for future breeding programs, including gene mapping. The results also showed that microsatellite markers associated to genes or QTLs controlling growth duration properties are suitable tools for marker assisted selection (MAS) to select rice lines with short growth duration. DOI: http://dx.doi.org/10.3329/ralf.v1i1.22354 Res. Agric., Livest. Fish.1(1): 37-46, Dec 2014

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