scholarly journals Role of amylase and protease in germinatingSterculia urens Roxb

2020 ◽  
Vol 55 (2) ◽  
pp. 107-112
Author(s):  
S Botcha ◽  
SD Prattipati
Keyword(s):  
Amino Acid ◽  
Seed Germination ◽  
Growth And Development ◽  
Free Amino ◽  
Amylase Activity ◽  
Amino Acid Content ◽  
Free Amino Acid Content ◽  
Initial Stage ◽  
Sterculia Urens

The present study explains the levels of proteins and enzymes like proteases and amylases associated with the breakdown of proteins and carbohydrates during various stages (0 day to 15th day) of seed germination of Sterculia urens Roxb.. Maximum protease activity (1.12 units/mg of protein) and amylase activity was observed on 12thday of seed germination (34 units/mg of protein) and decreased thereafter. Highest protein content was observed at initial stage of seed germination and decreased thereafter. Increased proteolytic activity and amylase activity proportionately increases free amino acid content and sugars that promotes the seedlings growth and development. Bangladesh J. Sci. Ind. Res.55(2), 107-112, 2020

Evaluation of seed amino acid content and its correlation network analysis in wild soybean (Glycine soja) germplasm in Japan

2021 ◽  
Vol 19 (1) ◽  
pp. 35-43
Author(s):  
Awatsaya Chotekajorn ◽  
Takuyu Hashiguchi ◽  
Masatsugu Hashiguchi ◽  
Hidenori Tanaka ◽  
Ryo Akashi
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Acid Content ◽  
Free Amino ◽  
Glycine Soja ◽  
Wild Soybean ◽  
Amino Acid Content ◽  
Soybean Seeds ◽  
Free Amino Acid Content

AbstractWild soybean (Glycine soja) is a valuable genetic resource for soybean improvement. Seed composition profiles provide beneficial information for the effective conservation and utilization of wild soybeans. Therefore, this study aimed to assess the variation in free amino acid abundance in the seeds of wild soybean germplasm collected in Japan. The free amino acid content in the seeds from 316 accessions of wild soybean ranged from 0.965 to 5.987 mg/g seed dry weight (DW), representing a 6.2-fold difference. Three amino acids had the highest coefficient of variation (CV): asparagine (1.15), histidine (0.95) and glutamine (0.94). Arginine (0.775 mg/g DW) was the predominant amino acid in wild soybean seeds, whereas the least abundant seed amino acid was glutamine (0.008 mg/g DW). A correlation network revealed significant positive relationships among most amino acids. Wild soybean seeds from different regions of origin had significantly different levels of several amino acids. In addition, a significant correlation between latitude and longitude of the collection sites and the total free amino acid content of seeds was observed. Our study reports diverse phenotypic data on the free amino acid content in seeds of wild soybean resources collected from throughout Japan. This information will be useful in conservation programmes for Japanese wild soybean and for the selection of accessions with favourable characteristics in future legume crop improvement efforts.

scholarly journals Free amino acid content in muscle tissue of bighead carp and wels catfish

2016 ◽  
Vol 72 (10) ◽  
pp. 632-636 ◽  
Author(s):  
Renata Pyz-Łukasik ◽  
Maria Szpetnar ◽  
Waldemar Paszkiewicz ◽  
Marcin R. Tatara ◽  
Adam Brodzki
Keyword(s):  
Amino Acid ◽  
Free Amino Acid ◽  
Muscle Tissue ◽  
Acid Content ◽  
Free Amino ◽  
Amino Acid Content ◽  
Bighead Carp ◽  
Predatory Fish ◽  
Free Amino Acid Content ◽  
Natural Diet

The present research was aimed at a comparative analysis of free amino acid (FAA) levels in the muscle tissue of herbivorous and predatory fish. The FAA concentration in the muscle tissue samples from bighead carp and wels catfish was determined by ion-exchange chromatography. The bighead carp muscle tissue, as compared to that of wels catfish, showed significantly higher concentrations of arginine, histidine, methionine, phenyloalanine, alanine, asparagine, serine, glycine, and taurine, but significantly lower levels of isoleucine, leucine, threonine, valine, glutamine, cystationine, β-alanine, ethanoloamine, as well as aspartic, glutamic, cysteic, α- and γ-aminobutyric acids. The muscle tissues of bighead carp and wels catfish did not differ significantly in the levels of cystine, lysine, tryptophan, tyrosine, α-aminoadipic acid, cytrulline, ornithine and 1-methyl-histidine. Proline was detected only in the wels catfish muscle. The results obtained have shown differences in free amino acid concentration in muscle tissue of examined fish. A differentiated natural diet induces changes in free amino acid content in fish tissues. Knowledge of levels of free amino acids which are precursors for biogenic amines facilitates setting the safety criteria for fish and fishery products from species other than those mentioned in the Commission Regulation No 2073/2005

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