scholarly journals Morphological and Molecular Characterization of Several Neogastropod Species (Mollusca: Gastropoda) From Coastal Waters of Bangladesh With One New Record

2021 ◽  
pp. 79-91
Author(s):  
KS Sultana ◽  
PS Brishti ◽  
S Ahmed ◽  
MB Billah ◽  
KA Habib
Keyword(s):  
Molecular Characterization ◽  
New Record ◽  
Gc Content ◽  
Coi Gene ◽  
The South ◽  
First Time ◽  
Mitochondrial Cytochrome Oxidase ◽  
Two Parameter ◽  
Morphological And Molecular Characterization

The present study reports the morphological and molecular characterization of marine neogastropods collected from the South-East to the South-West coasts of Bangladesh. A total of 21 species under 13 families were identified morphologically, of which 7 species were barcoded successfully using partial sequence of mitochondrial cytochrome oxidase subunit-I (COI) gene. The average nucleotide frequencies of these species were G (guanine) = 20.57%, C (cytosine) = 18.44%, A (adenine) = 23.65%, T (thymine) = 37.35% and the GC content was 39.01%. The average Kimura two-parameter (K2P) distances of the COI barcode sequences within species, genera, and families were 20.7%, 22.0%, and 22.0%, respectively. The average inter-species genetic divergence was calculated as 21.0%. In addition, the COI barcode sequence of Nassarius stolatus was identified and submitted to the GenBank for the first time. The study also reports the new record of a species, Indothais rufotincta Tan & Sigurdsson, 1996 from Bangladesh. This finding greatly extends the distributional range of this species from the West coast to the East coast of the Bay of Bengal. J. Bio-Sci. 29(1): 79-91, 2021 (June)

scholarly journals Morphological and molecular characterization of Haplorchoides mehrai Pande and Shukla 1976 (Digenea: Heterophyidae) from Chiang Mai province

2018 ◽  
Vol 55 (4) ◽  
pp. 334-342
Author(s):  
K. Apiwong ◽  
Ch. Wongsawad ◽  
P. Butboonchoo
Keyword(s):  
Phylogenetic Analysis ◽  
New Record ◽  
Adult Stage ◽  
Morphological Characteristics ◽  
Molecular Characteristic ◽  
Coi Gene ◽  
Chiang Mai ◽  
Chiang Mai Province ◽  
Morphological And Molecular Characterization

Abstract Cyprinoid fish in Chiang Mai province has been reported the presence of a large number of metacercariae, particularly the metacercariae of Haplorchoides and those not identified to species. This study aims to investigate morphological and molecular characteristic of the minute intestinal fluke H. mehrai metacercariae in two cyprinoid fish species from Chom Thong district, Chiang Mai province, Thailand: the Tinfoil barb (Barbonymus schwanenfeldii) and the White eye barb (Cyclocheilichthys repasson). A total of 180 fish (90 from B. schwanenfeldii and 90 from C. repasson) were collected over three seasons: cool, hot and the rainy season (December 2015 to August 2016). Fish were examined for H. mehrai metacercariae infection, including areas such as muscle and the inner side of body scales, by using a light microscope. The prevalence of H. mehrai metacercariae in B. schwanenfeldii and C. repasson was 73.33 % and 100 % respectively. Haplorchoides metacercariae were identified as H. mehrai based on the morphological characteristics; the position of the acetabulum and the number and arrangement of the acetabular spines. Phylogenetic analysis based on Cytochrome c Oxidase subunit I (COI) gene showed that H. mehrai metacercariae from B. schwanenfeldii and C. repasson were the same species as the adult stage of H. mehrai from Hemibagrus nemurus and Mystus multiradiatus. Both morphological and molecular characteristic could indicate that Haplorchoides metacercariae originated from this study were H. mehrai. Furthermore, it is a new record of the minute intestinal fluke Haplorchoides mehrai in Chiang Mai Province

scholarly journals Short Communication New record of Indian oil sardine Sardinella longiceps from the coastal region of Bangladesh

Ocean Life ◽  
2017 ◽  
Vol 1 (1) ◽  
pp. 11-13
Author(s):  
SHAMSUNNAHAR SHAMSUNNAHAR ◽  
MOHAMMAD ABDUL BAKI ◽  
ANIRBAN SARKER ◽  
MOST. HASINA BEGUM ◽  
A. B.M. ZAFARIA ◽  
...  
Keyword(s):  
New Record ◽  
Coastal Region ◽  
First Record ◽  
Coi Gene ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Tissue Samples ◽  
Sardinella Longiceps ◽  
Mitochondrial Cytochrome Oxidase ◽  
Meristic Data

Shamsunnahar, Baki MA, Sarker A, Hasina Begum M, Zafaria M, Islam NN, Ahmed MdS. 2017. New record of Indian oil sardine Sardinella longiceps from the coastal region of Bangladesh. Ocean Life 1: 11-13. We report the first record of Indian oil sardine Sardinella longiceps from the southern coast of the Bay of Bengal, Bangladesh. The sample specimens were collected from Pathorghata, Barguna, Bangladesh on 25th October, 2015. Morphometric and meristic studies were performed for taxonomic identification. Genomic DNA was extracted from tissue samples and mitochondrial Cytochrome Oxidase Subunit I (COI) gene was amplified for molecular characterization of this species. The morphometric and meristic data and DNA barcoding confirm the presence of S. longiceps in Bangladesh. This report updates the geographical distribution for this species confirming its presence in the coastal region of Bangladesh, and extends the number of marine fish known from the area.

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