scholarly journals Morphological and molecular characterization of Haplorchoides mehrai Pande and Shukla 1976 (Digenea: Heterophyidae) from Chiang Mai province

2018 ◽  
Vol 55 (4) ◽  
pp. 334-342
Author(s):  
K. Apiwong ◽  
Ch. Wongsawad ◽  
P. Butboonchoo
Keyword(s):  
Phylogenetic Analysis ◽  
New Record ◽  
Adult Stage ◽  
Morphological Characteristics ◽  
Molecular Characteristic ◽  
Coi Gene ◽  
Chiang Mai ◽  
Chiang Mai Province ◽  
Morphological And Molecular Characterization

Abstract Cyprinoid fish in Chiang Mai province has been reported the presence of a large number of metacercariae, particularly the metacercariae of Haplorchoides and those not identified to species. This study aims to investigate morphological and molecular characteristic of the minute intestinal fluke H. mehrai metacercariae in two cyprinoid fish species from Chom Thong district, Chiang Mai province, Thailand: the Tinfoil barb (Barbonymus schwanenfeldii) and the White eye barb (Cyclocheilichthys repasson). A total of 180 fish (90 from B. schwanenfeldii and 90 from C. repasson) were collected over three seasons: cool, hot and the rainy season (December 2015 to August 2016). Fish were examined for H. mehrai metacercariae infection, including areas such as muscle and the inner side of body scales, by using a light microscope. The prevalence of H. mehrai metacercariae in B. schwanenfeldii and C. repasson was 73.33 % and 100 % respectively. Haplorchoides metacercariae were identified as H. mehrai based on the morphological characteristics; the position of the acetabulum and the number and arrangement of the acetabular spines. Phylogenetic analysis based on Cytochrome c Oxidase subunit I (COI) gene showed that H. mehrai metacercariae from B. schwanenfeldii and C. repasson were the same species as the adult stage of H. mehrai from Hemibagrus nemurus and Mystus multiradiatus. Both morphological and molecular characteristic could indicate that Haplorchoides metacercariae originated from this study were H. mehrai. Furthermore, it is a new record of the minute intestinal fluke Haplorchoides mehrai in Chiang Mai Province

scholarly journals Morphological and Molecular Characterization of Several Neogastropod Species (Mollusca: Gastropoda) From Coastal Waters of Bangladesh With One New Record

2021 ◽  
pp. 79-91
Author(s):  
KS Sultana ◽  
PS Brishti ◽  
S Ahmed ◽  
MB Billah ◽  
KA Habib
Keyword(s):  
Molecular Characterization ◽  
New Record ◽  
Gc Content ◽  
Coi Gene ◽  
The South ◽  
First Time ◽  
Mitochondrial Cytochrome Oxidase ◽  
Two Parameter ◽  
Morphological And Molecular Characterization

The present study reports the morphological and molecular characterization of marine neogastropods collected from the South-East to the South-West coasts of Bangladesh. A total of 21 species under 13 families were identified morphologically, of which 7 species were barcoded successfully using partial sequence of mitochondrial cytochrome oxidase subunit-I (COI) gene. The average nucleotide frequencies of these species were G (guanine) = 20.57%, C (cytosine) = 18.44%, A (adenine) = 23.65%, T (thymine) = 37.35% and the GC content was 39.01%. The average Kimura two-parameter (K2P) distances of the COI barcode sequences within species, genera, and families were 20.7%, 22.0%, and 22.0%, respectively. The average inter-species genetic divergence was calculated as 21.0%. In addition, the COI barcode sequence of Nassarius stolatus was identified and submitted to the GenBank for the first time. The study also reports the new record of a species, Indothais rufotincta Tan & Sigurdsson, 1996 from Bangladesh. This finding greatly extends the distributional range of this species from the West coast to the East coast of the Bay of Bengal. J. Bio-Sci. 29(1): 79-91, 2021 (June)

scholarly journals First record of Borofutus dhakanus (Boletaceae, Leccinoideae) in Thailand

2017 ◽  
Vol 69 (3) ◽  
pp. 545-552
Author(s):  
Sutheera Thongkantha ◽  
Sulichet Thongklam ◽  
Wasu Pathom-Aree ◽  
Saisamorn Lumyong ◽  
Boonsom Bussaban
Keyword(s):  
Internal Transcribed Spacer ◽  
New Record ◽  
Type Species ◽  
Morphological Characteristics ◽  
Taxonomic Revision ◽  
First Record ◽  
Morphological Characters ◽  
Chiang Mai ◽  
Its Gene ◽  
Chiang Mai Province

A new record of Borofutus dhakanus Hosen & Zhu L. Yang is reported from Chiang Mai Province, northern Thailand. The phylogenetic relationships of the Thai specimen were compared with those of the type species and allied genera from seven major subfamilies of Boletaceae. The trees were constructed using sequences of partial 28S rDNA combined with internal transcribed spacer (ITS) gene regions. The morphological characteristics of the fungus and its edibility are also discussed. Although many boletes have been documented from Thailand, most previous records are based only on morphological characters and there is an urgent need for taxonomic revision.

scholarly journals Characterization of Alternaria isolates causing leaf spots in radish in Brazil

2020 ◽  
Vol 46 (4) ◽  
pp. 340-341
Author(s):  
Cléia Santos Cabral ◽  
Elenice Alves Barboza ◽  
Luiz Henrique Rocha Lopes ◽  
Maurício Rossato ◽  
Rafaela Cristina Ferreira Borges ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Morphological Characteristics ◽  
Phytopathogenic Fungus ◽  
Morphological Markers ◽  
Phylogenetic Species ◽  
Koch’S Postulates ◽  
First Report ◽  
Leaf Spots ◽  
Necrotic Spots

ABSTRACT Alternaria japonica Yoshii, an important cruciferous phytopathogenic fungus, has been identified in radish plants showing symptoms of necrotic spots with chlorotic halos. The samples were collected from the cities of Brasília-DF and Guaraciaba do Norte-CE, Brazil. The isolates are deposited in the collection of fungi and oomycetes of “Embrapa Hortaliças”. Using the concept of morphological and phylogenetic species, two isolates were selected (EH-945 and EH-1379) for identification. Through the evaluation of morphological markers, the isolates were concluded to be similar to A. japonica. Based on the phylogenetic analysis, the isolates grouped with A. japonica reference isolates ATCC 13618 and CBS 118390. To complete Koch’s postulates, radish, arugula, mustard and turnip plants were inoculated. All species showed symptoms similar to those originally reported in the field (except for non-inoculated controls) seven to 12 days after inoculation. The isolates obtained from symptomatic plants showed morphological characteristics identical to those of the pathogen. This is the first report of radish as a host of A. japonica in Brazil.

Hydnaceous fungi of China 7. Morphological and molecular characterization of Phellodon subconfluens sp. nov. from temperate, deciduous forests

Phytotaxa ◽  
2019 ◽  
Vol 414 (6) ◽  
pp. 280-288 ◽  
Author(s):  
YAN-HONG MU ◽  
FANG WU ◽  
HAI-SHENG YUAN
Keyword(s):  
New Species ◽  
Morphological Characteristics ◽  
Phylogenetic Position ◽  
Closely Related Species ◽  
Rdna Its ◽  
Rdna Its Sequences ◽  
Temperate Deciduous ◽  
Temperate Deciduous Forests ◽  
Morphological And Molecular Characterization

A new hydnaceous fungus, Phellodon subconfluens, from northeast China, is described and illustrated based on morphological characteristics and rDNA ITS sequences. The new species is characterized by circular to flabelliform basidiocarps, a greyish buff, brownish orange to reddish brown and obscurely zonate pileal surface with white, incurved margins, a monomitic hyphal system with simple-septate, generative hyphae, and broadly ellipsoid to subglobose, thin-walled basidiospores with echinulate ornamentation. Molecular analysis confirms the phylogenetic position of the new species in Phellodon. The discriminating characters of the new species and closely related species are discussed.

A new record of Ornithodoros (Pavlovskyella) verrucosus (Acari: Argasidae) from a porcupine burrow in western Iran

2019 ◽  
Vol 24 (5) ◽  
pp. 771
Author(s):  
Asadollah Hosseini Chegeni ◽  
Majid Tavakoli ◽  
Habibollah Koshki ◽  
Javad Khedri ◽  
Mohammad Hassan Kayedi
Keyword(s):  
Cytochrome Oxidase ◽  
18S Rdna ◽  
Genomic Dna ◽  
New Record ◽  
Morphological Characteristics ◽  
Species Group ◽  
Coi Gene ◽  
Cytochrome Oxidase Subunit ◽  
Western Iran ◽  
Blast Analysis

We found an Ornithodoros species with distinct morphological characteristics than Alveonasus genus and other O. (Pavlovskyella) species group. Tick specimens were collected from a porcupine burrow, located in Lorestan province, western Iran. Genomic DNA of tick was extracted using CTAB and then three gene fragments of cytochrome oxidase subunit I (COI) and 16S mtDNA & 18S rDNA were amplified by PCR. Tick specimens were identified as near to O. (P.) verrucosus and O. (P.) tholozani. So the identification was confirmed by BLAST analysis of 16S mtDNA & 18S rDNA and COI gene fragments. A taxonomic key to the native adult subfamily Ornithodorinae species of Iran are provided to facilitate their identification.

Morphological and molecular characterization of Alternaria spp. isolated from European pears

Plant Disease ◽  
2021 ◽  
Author(s):  
Joseph DeShields ◽  
Achala KC
Keyword(s):  
Phylogenetic Analysis ◽  
Differential Susceptibility ◽  
Morphological Diversity ◽  
Plasma Membrane Atpase ◽  
Membrane Atpase ◽  
Morphological Differences ◽  
Postharvest Rot ◽  
Ncbi Blast ◽  
Morphological And Molecular Characterization

In a recent survey of postharvest rot pathogens in European pear in Southern Oregon, Alternaria spp. were frequently isolated from orchard samples of pear flowers and fruits. Morphological differences were observed within the isolated cultures. A preliminary NCBI BLAST search analysis using sequences of the ATPase locus across 94 isolates of Alternaria spp. obtained from pear fruit rots, revealed three major Alternaria sections, sect. Alternata, sect. Infectoriae, and sect. Ulocladioides. Thirteen isolates were selected based on their genetic and morphological diversity across three Alternaria sections and were subjected to multilocus phylogenetic analysis using sequences from plasma membrane ATPase, calmodulin, and Alt a1 loci. Within section Alternata, four A. arborescens isolates and one A. destruens isolate were identified; within sections Infectoriae and Ulocladioides, one A. rosae isolate and two A. botrytis isolates were identified, respectively. The remaining five isolates could not be identified based on the available sequences for the three loci used in this study. In addition to the phylogenetic analysis, pathogenicity assays revealed differential responses to these isolates on four pear cultivars Anjou, Bartlett, Comice, and Bosc. Inoculation of isolates within Alternaria sect. Alternata resulted in fruit lesions across all cultivars with Bosc being significantly susceptible (p<0.0001). Isolates within Alternaria sect. Ulocladioides caused rots on Anjou and Bosc, while isolates within Alternaria sect. Infectoriae developed rots on Bosc only. This study suggests that there is differential susceptibility of pear cultivars to Alternaria rots and the severity of postharvest rot depends on the type of Alternaria spp. and cultivar predominant in a region.

scholarly journals DNA barcoding of razor clam Solen spp. (Solinidae, Bivalva) in Indonesian beaches

2020 ◽  
Vol 21 (2) ◽  
Author(s):  
Ninis trisyani Margono ◽  
DWI ANGGOROWATI RAHAYU
Keyword(s):  
Phylogenetic Analysis ◽  
Genetic Distance ◽  
Dna Barcoding ◽  
Dna Barcode ◽  
Morphological Characteristics ◽  
Wide Distribution ◽  
Coi Gene ◽  
Razor Clam ◽  
Molecular Phylogenetic ◽  
Solen Grandis

Abstract. Trisyani N, Rahayu DA. 2020. DNA barcoding of razor clam Solen spp. (Solinidae, Bivalva) in Indonesian beaches. Biodiversitas 21: 478-484. Solen spp. are shells with various morphological characteristics with a wide distribution of tropical and subtropical beaches, including Indonesia. The identification of Solen spp. is generally based on its morphological characteristics. This method is very problematic due to specimens share similarity in morphology and color. This study was using DNA barcode as a molecular identification tool. The bivalve COI sequence was amplified using PCR and molecular phylogenetic analysis using the Neighbor-Joining method. The amplified COI gene has a length of about 665 bp. The purpose of this study was to evaluate genetic variation and compare the phylogenetic Solen spp. in Indonesian waters. The composition of the nucleotide bases of Solen spp. the comparative species are A = 26.79%, C = 23.16%, G = 19.17% and T = 30.93%. The total nucleotide base A + T was 57.72%, while G + C was 42.33%. The results of phylogenetic analysis showed that Solen spp. Cirebon and Jambi are in one clade with Solen regularis with genetic distance 0.000 - 0.002. Solen spp. Surabaya, Bangkalan, Pamekasan, and Sumenep are in separate clades and are related to Solen grandis, Solen stricus and Solen lamarckii with genetic distance from 0.146 - 0.156. The diversity of nucleotide was 0.9780 and was divided into 12 haplotypes.

scholarly journals Golovinomyces asperifolii: first record in China and Bothriospermum chinense as a new host

Mycotaxon ◽  
2020 ◽  
Vol 135 (2) ◽  
pp. 309-313
Author(s):  
Luchao Bai ◽  
Huiyan Xiong ◽  
Baoguo Shi
Keyword(s):  
Phylogenetic Analysis ◽  
New Record ◽  
Morphological Characteristics ◽  
First Record ◽  
Qilian Mountains ◽  
New Host ◽  
Qinghai Province ◽  
Rdna Its ◽  
The Qilian Mountains

Golovinomyces asperifolii is described from the Qilian mountains in Qinghai province as a new record in China. The identification is supported by morphological characteristics and phylogenetic analysis of rDNA ITS. The fungus was collected on Bothriospermum chinense, which represents a new host for G. asperifolii.

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