scholarly journals Micropropagation of Marsdenia brunoniana Wight & Arn. - A Rare Antidiabetic Plant

2012 ◽  
Vol 21 (1) ◽  
pp. 89-93
Author(s):  
A. Ugraiah ◽  
S. Karuppusamy ◽  
T. Pullaiah
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Growth Regulators ◽  
Plant Tissue ◽  
Short Communication ◽  
Shoot Multiplication ◽  
Nodal Explants ◽  
Plant Micropropagation ◽  
Number Of Shoots

Shoot multiplication of Marsdenia brunoniana was obtained from the nodal explants of mature plants using MS with different concentrations and combinations of growth regulators. Maximum explant response and highest number of shoots per explant was obtained on MS fortified with 1.0 mg/l BAP. The highest degree of shoot profilieration was found to be 90%. The combination of BAP and Kn was also found to be effective for regeneration. The regenerated shoots were successfully rooted on MS supplemented with 0.5 mg/l NAA, after sequential hardening, survival rate was 90%.   Key words: Marsdenia brunoniana, Antidiabetic plant, Micropropagation, Conservation   D. O. I. 10.3329/ptcb.v21i1.9613   Plant Tissue Cult. & Biotech. 21(1): 89-93, 2011 (June) - Short communication

scholarly journals Micropropagation of Marsdenia brunoniana Wight & Arn. - A rare antidiabetic plant

2010 ◽  
Vol 20 (1) ◽  
pp. 7-12 ◽  
Author(s):  
A. Ugraiah ◽  
S. Karuppusamy ◽  
T. Pullaiah
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Growth Regulators ◽  
Plant Tissue ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Nodal Explants ◽  
Ms Medium ◽  
Plant Micropropagation ◽  
Number Of Shoots

Shoot multiplication of M. brunoniana Wight & Arn. was achieved from the nodal explants of mature plants using MS with different concentrations and combina-tions of growth regulators. Maximum explant response and highest number of shoots per explant was obtained on MS medium fortified with 1.0 mg/l BAP. The highest degree of shoot proliferation was found to be 90%. The combination of BAP and Kn was also found to be effective for regeneration. The regenerated shoots were successfully rooted on MS supplemented with 0.5 mg/l NAA, after sequential hardening; survival rate was 90%.  Key words: Marsdenia brunoniana, Medinal plant, Micropropagation, Conservation D. O.I. 10.3329/ptcb.v20i1.5958 Plant Tissue Cult. & Biotech. 20(1): 7-12, 2010 (June)

scholarly journals Effects of Growth Regulators on In vitro Propagation and Tuberization of Four Dioscorea Species

1970 ◽  
Vol 18 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Md. Tariqul Islam ◽  
E. R. Joachim Keller ◽  
D. Philibert Dembele
Keyword(s):  
Key Words ◽  
Growth Regulators ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
In Vitro Production ◽  
Dioscorea Species ◽  
Vitro Production ◽  
Species Being ◽  
Nodal Culture

Nodal explants of 12 accessions from four species of yam (Dioscorea spp.) were cultured for six weeks on MS to evaluate the influence of IAA, Kn, NAA and BAP on the production of leaves and microtubers. Four Dioscorea polystachya Turcz., three each of D. bulbifera L. and D. sansibarensis Pax. and two D. japonica Thunb. accessions were used. Five and 10 mg/l of Kn along with IAA and sucrose, and 0.2 and 0.5 mg/l of NAA, sucrose and with or without BAP were used in four treatments. The accessions Yam 23 and Yam 25 of D. sansibarensis failed to initiate any leaf under four treatments. The remaining accessions produced 0.11 to 1.76 leaves per explant. The medium containing IAA with higher concentration of Kn (10 mg/l) and 3% sucrose was found to be best for in vitro production of leaf (0.71/explant) and the most productive species was D. japonica (1.36), followed by D. polystachya (1.19/explant). At the same culture period, Yam 16 of D. bulbifera failed to initiate any microtuber at IAA with Kn, and NAA with or without BAP. The remaining accessions produced 0.09 to 1.15 microtubers per explant. Lower concentration of Kn (5 mg/l) with IAA and sucrose was favourable for producing microtubers (0.61/explant on an average), the best species being D. sansibarensis (1.27) followed by D. japonica (0.59/ explant). Finally, the presence of BAP adversely affected the production of microtuber among Dioscorea species. Key words: Dioscorea spp., Microtuber, Nodal culture, Propagation D.O.I. 10.3329/ptcb.v18i1.3260 Plant Tissue Cult. & Biotech. 18(1): 25-35, 2008 (June)

scholarly journals In vitro Propagation of Solanum capsicoides All. – An Important Therapeutic Agent 'Kantakari'

1970 ◽  
Vol 20 (2) ◽  
pp. 179-184
Author(s):  
N.P. Anish ◽  
M.G. Rajesh ◽  
Jiby Elias ◽  
N. Jayan
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Therapeutic Agent ◽  
Shoot Tip ◽  
Shoot Induction ◽  
Half Strength ◽  
Shoot Tip Explants

Shoot tip explants from in vitro germinated seedlings of Solanum capsicoides All. inoculated on MS containing 2 mg/l BA produced maximum shoot induction response (26 shoots per explant). Rooting of the microshoots (19.4 roots per explant) was obtained better in half strength of MS supplemented with NAA (0.5 mg/l). Well rooted plantlets were successfully hardened with 80 per cent survival rate.   Key words: Solanum capsicoides, Propagation, Therapeutic agent   D.O.I. 10.3329/ptcb.v20i2.6912   Plant Tissue Cult. & Biotech. 20(2): 179-184, 2010 (December)

scholarly journals Asexual Propagation of Eggplant (Solanum melongena L.) through Encapsulated Axillary Buds

1970 ◽  
Vol 19 (2) ◽  
pp. 263-288 ◽  
Author(s):  
A.K.M. N. Huda ◽  
, M.A. Bari ◽  
M. Rahman
Keyword(s):  
Key Words ◽  
Plant Tissue ◽  
Short Communication ◽  
Solanum Melongena ◽  
Axillary Buds ◽  
Synthetic Seed ◽  
Asexual Propagation

 Key words: Auxillary buds, Eggplant, Encapsulation, Synthetic seed D.O.I. 10.3329/ptcb.v19i2.5445 Plant Tissue Cult. & Biotech. 19(2): 263-288, 2009 (December)       - Short communication

scholarly journals Assessment of In-vitro culture through Nodal explants of Dendrocalamus hamiltonii

2021 ◽  
Vol 2 (1) ◽  
pp. 130-133
Author(s):  
Abha Jha ◽  
◽  
Sunila Das ◽  
Keyword(s):  
Experimental Study ◽  
Growth Regulators ◽  
In Vitro Regeneration ◽  
Shoot Multiplication ◽  
Growth Hormones ◽  
Nodal Explants ◽  
Bud Break ◽  
Multiplication Rate ◽  
Dendrocalamus Hamiltonii

The present experimental study was aimed to overcome the traditional methods of propagation that limit the number of propagules by in-vitro regeneration through nodal explants of Dendrocalamus hamiltonii with a comparative study of growth regulators during the shooting and rooting process. Dendrocalamus hamiltonii is distributed from the Himalayas (Nepal) to the northern part of Burma. Collection of explants was done from different selected sites of CPTs. There was the use of HgCl2 and Ca (OCl)2 as sterilizing agents in different concentrations and its effect was visualized during the sprouting stage. Culm explants were cultured in a bottle containing White media (Wm) supplemented with BA and Kinetin for sprouting and IAA, IBA, NAA for rooting. There is also the use of IAA+IBA+NAA in combined form as a supplementary solution 0.1% HgCl2 treatment for 20-minute results into77.80% aseptic buds and 72% bud -break. Among the used growth-hormones, BA with concentration 0.25mg/l and 0.50mg/l respectively were appropriate for shoot-multiplication rate, 4.01±0.3 and 4.3±0.4 were ideal observation incorporation with BA (1.00mg/l) and BA (1.50mg/l) respectively. Maximum sprouting rate14.77±3.37with application of BA (2.00mg/l) and maximum shoot length4.3±0.4 is observed at BA (1.50mg/l). The applications of rooting hormone IAA+IBA+NAA in the concentration of 1.0 mg/l results in 72.5±0.3(rooting) and 11.1±0.3 (av. No. of the root).

scholarly journals Vacuolar Na+/H+ Antiporter Expression and Salt Tolerance Conferred by Actin1D and CaMV35S are Similar in Transgenic Binnatoa Rice

1970 ◽  
Vol 19 (2) ◽  
pp. 257-262 ◽  
Author(s):  
S.M. Touhidul Islam ◽  
Zeba I. Seraj
Keyword(s):  
Salt Tolerance ◽  
Transgenic Plant ◽  
Key Words ◽  
Plant Tissue ◽  
Short Communication ◽  
Rt Pcr ◽  
Ion Imbalance

Key words:  RT-PCR, ion imbalance, Binnatoa, transgenic plant  D.O.I. 10.3329/ptcb.v19i2.5444 Plant Tissue Cult. & Biotech. 19(2): 257-262, 2009 (December)       - Short communication

scholarly journals THE ROLL OF SOME PLANT GROWTH REGULATORS ON SHOOTS MULTIPLICATION OF STEVIA PLANTS IN VITRO

2017 ◽  
Vol 48 (5) ◽  
Author(s):  
Al-Obaidy & Khierallah
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Culture Media ◽  
Stevia Rebaudiana ◽  
Shoot Multiplication ◽  
Dry Weight ◽  
Fresh Weight ◽  
Number Of Shoots

This research was conducted to study the effect of some plant growth regulators on in vitro shoots multiplication of stevia (Stevia rebaudiana Bertoni). The experiments included tests of various combinations of KIN with IBA or IAA in the shoot multiplication. Results indicated that KIN at 1.0 mg. L-1 plus 0.3 mg. L-1 of IBA produced the highest number of shoots (3.5 shoots) while KIN at 1.5 mg. L-1 plus IBA at 1.0 mg. L-1 produced the lowest shoot length (1.14 cm).  Hormone free medium produced the highest rate of the leaves number reached 28.56 leaves. KIN and IBA interaction increased fresh and dry weight significantly.   Treatment contained 2.0 mg -1 KIN plus 0.3 mg. L-1 IBA produced the highest fresh weight (1.739 g) while 0.5 mg. L-1 KIN and 0.3 mg. L-1 IBA produced the highest dry weight (0.822 g). As for the effect of interaction between the IAA and KIN it was significant in the number of shoots formed. Interaction between 1.0 mg. L-1 KIN with 0.1 mg. L-1IAA produced the highest number of shoots (3.8 shoots). Shoots length reached 8.10 cm in the media with 0.3 mg. L-1 IAA only. The highest fresh weight (1.267 g) was achieved with the interaction between 1.0 mg. L-1 KIN and 0.3 mg. L-1 IAA while 0.5 mg. L-1IAA without KIN produced the highest dry weight reached 0.138 g.  Shoots multiplication was improved by incorporation of the cytokinin TDZ in culture media. Shoots number, fresh and dry weights were increased significantly by adding 0.05 mg. L-1 of TDZ at present of 0.3 mg. L-1 of IBA giving 6.6 shoots, 0.974 g and 0.144 g respectively while shoots length decreased significantly as media without TDZ produced the highest shoots length reached 9.32 cm. The above results can adopt for the successful in vitro shoot multiplication of Stevia plants. 

scholarly journals In Vitro Study of Callus Induction in Wedelia trilobata Using Different Growth Regulators

2012 ◽  
Vol 21 (1) ◽  
pp. 95-99 ◽  
Author(s):  
Tripti Thakur ◽  
Rakesh Dadsena ◽  
S. B. Nandeshwar ◽  
Anil Kumar
Keyword(s):  
Central America ◽  
Growth Regulators ◽  
Callus Induction ◽  
Plant Tissue ◽  
Short Communication ◽  
In Vitro Study ◽  
Narrow Sense ◽  
Wedelia Trilobata ◽  
Hot Climate

Wedelia trilobata (L.) Hitch is a member of Asteraceae (formerly Compositae), the sunflower family. Its common name includes “Wedelia” and trailing daisy, although some people mistakenly call it Singapore daisy. In a wider sense the genus Wedelia, named in honor of Georg Wolfgang Wedel (1645 –1721), Professor of Botany at Jena, Germany, has about 70 species of tropical strand plant, Wollastonia biflora (formerly known as Wedelia biflora), to which it is closely related. In a more narrow sense, and now more widely accepted, Wedelia is considered to be a genus of relatively a few species of strictly American origin, one of which is Wedelia parviceps Blake, a herbaceous or sub-shrubby weed common in croplands, harvested fields, pasteures and roadside bush in hot climate in central America (Garcia et al. 1975). Interestingly, the Hawaiian endemic genus Lipochaeta is scarcely distinct from it genetically, and two sections of Lipochaeta appears to have been independently derived from Wedelia like ancestors (Wagner et al. 1990).   Key words: Wedelia trilobata, Callus induction, In vitro   D. O. I. 10.3329/ptcb.v21i1.9614   Plant Tissue Cult. & Biotech. 21(1): 95-99, 2011 (June) - Short communication

scholarly journals In vitro Mass Propagation from Shoot Tip Explants of Vernonia cinerea (L.) Less. An Antioxidant, Anti-inflammatory Medicinal Plant

1970 ◽  
Vol 20 (2) ◽  
pp. 127-131 ◽  
Author(s):  
M. Maharajan ◽  
Abdul Bakrudeen Ali Ahmed ◽  
Rosna Mat Taha ◽  
S. Jawahar ◽  
R. Ravi Paul ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Key Words ◽  
Plant Tissue ◽  
Shoot Tip ◽  
Mass Propagation ◽  
Anti Inflammatory ◽  
Shoot Tip Explants ◽  
Vernonia Cinerea ◽  
Number Of Shoots

Plantlets were regenerated from the shoot tip explants of Vernonia cinerea (L.) Less. in MS supplimented with BA and Kn. Maximum number of shoots in BA (13.32 mg/l) and roots in IBA (7.38 mg/l) developed. The rooted plantlets were successfully established in the field.   Key words: Vernonia cinerea, Mass propagation, Shoot tip explants, Antioxidant, Medicinal plant   D.O.I. 10.3329/ptcb.v20i2.6892   Plant Tissue Cult. & Biotech. 20(2): 127-131, 2010 (December)

scholarly journals Rapid Shoot Regeneration from Thin Cell Layer Explants of an Endangered Medicinal Asclepiad Ceropegia spiralis L.

2012 ◽  
Vol 21 (1) ◽  
pp. 63-73 ◽  
Author(s):  
K. Sri Rama Murthy ◽  
R. Kondamudi
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Plant Tissue ◽  
Cell Layer ◽  
Thin Cell Layer ◽  
Thin Cell Layers ◽  
In Vitro Plantlets ◽  
Cell Layers ◽  
Extensive Growth

The thin cell layers of nodes and internodes of Ceropegia spiralis L. were cultured on  MS  supplemented with BAP 13.32 µM/l + NAA 0.537 μM/l induced 17.34 ± 0.55 shoots showing extensive growth. Later on the organogenesis was also induced on MS containing BAP 13.32 µM/l + 2, 4-D 1.130 µM/l, whereas the medium with BAP 13.32 µM/l + 2, 4-D 4.52 µM/l has the highest callus producing ability in recalcitrants as well as in normal explants. Shoots developed were rooted best on 0.5 MS with NAA 10.74 μM/l. Optimum shoot and root multiplication was obtained within eight weeks.  In vitro plantlets were successfully weaned and transferred to soil with about 90 per cent survival rate. So far, more than 650 weanlings have been produced successfully and reintroduced into nature for their recovery.     Key words: Ceropegia spiralis, Thin cell layer explants, Recalcitrants, Regeneration   D. O. I. 10.3329/ptcb.v21i1.9564   Plant Tissue Cult. & Biotech. 21(1): 63-73, 2011 (June)

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