scholarly journals Effects of Growth Regulators on In vitro Propagation and Tuberization of Four Dioscorea Species

1970 ◽  
Vol 18 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Md. Tariqul Islam ◽  
E. R. Joachim Keller ◽  
D. Philibert Dembele
Keyword(s):  
Key Words ◽  
Growth Regulators ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
In Vitro Production ◽  
Dioscorea Species ◽  
Vitro Production ◽  
Species Being ◽  
Nodal Culture

Nodal explants of 12 accessions from four species of yam (Dioscorea spp.) were cultured for six weeks on MS to evaluate the influence of IAA, Kn, NAA and BAP on the production of leaves and microtubers. Four Dioscorea polystachya Turcz., three each of D. bulbifera L. and D. sansibarensis Pax. and two D. japonica Thunb. accessions were used. Five and 10 mg/l of Kn along with IAA and sucrose, and 0.2 and 0.5 mg/l of NAA, sucrose and with or without BAP were used in four treatments. The accessions Yam 23 and Yam 25 of D. sansibarensis failed to initiate any leaf under four treatments. The remaining accessions produced 0.11 to 1.76 leaves per explant. The medium containing IAA with higher concentration of Kn (10 mg/l) and 3% sucrose was found to be best for in vitro production of leaf (0.71/explant) and the most productive species was D. japonica (1.36), followed by D. polystachya (1.19/explant). At the same culture period, Yam 16 of D. bulbifera failed to initiate any microtuber at IAA with Kn, and NAA with or without BAP. The remaining accessions produced 0.09 to 1.15 microtubers per explant. Lower concentration of Kn (5 mg/l) with IAA and sucrose was favourable for producing microtubers (0.61/explant on an average), the best species being D. sansibarensis (1.27) followed by D. japonica (0.59/ explant). Finally, the presence of BAP adversely affected the production of microtuber among Dioscorea species. Key words: Dioscorea spp., Microtuber, Nodal culture, Propagation D.O.I. 10.3329/ptcb.v18i1.3260 Plant Tissue Cult. & Biotech. 18(1): 25-35, 2008 (June)

scholarly journals In vitro Propagation of Solanum capsicoides All. – An Important Therapeutic Agent 'Kantakari'

1970 ◽  
Vol 20 (2) ◽  
pp. 179-184
Author(s):  
N.P. Anish ◽  
M.G. Rajesh ◽  
Jiby Elias ◽  
N. Jayan
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Therapeutic Agent ◽  
Shoot Tip ◽  
Shoot Induction ◽  
Half Strength ◽  
Shoot Tip Explants

Shoot tip explants from in vitro germinated seedlings of Solanum capsicoides All. inoculated on MS containing 2 mg/l BA produced maximum shoot induction response (26 shoots per explant). Rooting of the microshoots (19.4 roots per explant) was obtained better in half strength of MS supplemented with NAA (0.5 mg/l). Well rooted plantlets were successfully hardened with 80 per cent survival rate.   Key words: Solanum capsicoides, Propagation, Therapeutic agent   D.O.I. 10.3329/ptcb.v20i2.6912   Plant Tissue Cult. & Biotech. 20(2): 179-184, 2010 (December)

scholarly journals An efficient in vitro propagation protocol for snowdrop anemone (Anemone sylvestris L.) 

2017 ◽  
Vol 44 (No. 4) ◽  
pp. 186-194 ◽  
Author(s):  
Jana Šedivá ◽  
Pavla Zahumenická ◽  
Eloy Fernández Cusimamani
Keyword(s):  
Plant Growth ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Growth Characteristics ◽  
Root Induction ◽  
Shoot Induction ◽  
Free Medium ◽  
In Vitro Production ◽  
Vitro Production

This study investigated in vitro production of diploid (AS2) and tetraploid (AS4) cytotypes of snowdrop anemone. The effect of plant growth regulators (PGRs) on in vitro shoot multiplication and rooting was investigated. The effect of activated charcoal (AC) on root induction was also studied. Ploidy level affected growth characteristics during multiplication and rooting. Shoot induction in AS4 was higher on medium supplemented with cytokinin (3.2–3.6), while the AS2 clone formed the most shoots on PGR-free medium (3.6). The highest rooting percentage was achieved on PGR-free medium in both genotypes (AS2 clone, 100% and AS4 clone, 93.3%). The addition of AC to the PGR media largely increased root induction and root length. Rooted plantlets were successfully acclimatised in the greenhouse with 100% survival. Thus, the described micropropagation protocol represents a rapid and effective in vitro propagation method for utilisation in horticulture and conservation programmes of snowdrop anemone.

scholarly journals In vitro production of capsaicin through plant tissue culture

2017 ◽  
pp. 24-33
Author(s):  
Swetnisha, Ajitabh Bora, H.K. Gogoi, P.S. Raju
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
In Vitro Production ◽  
Agricultural Produce ◽  
Medicinal Properties ◽  
Method Of Production ◽  
Culture Strategies ◽  
Vitro Production

Capsaicin, a secondary metabolite produced in capsicum, is in high demand in pharmaceutical industry because of its various medicinal properties. Currently, the supply of capsaicin depends upon its extraction from capsicum fruits. This limits the production of capsaicin as it depends upon agricultural produce. The current review has compiled information from various literature published on chemistry and importance of capsaicin along with its method of production. It also reviews the process of in vitro production of capsaicin through plant tissue culture, strategies of increasing capsaicin accumulation and its advantages over extraction from fruits and artificial synthesis.

scholarly journals In vitro Propagation and Ex vitro Rooting of Blueberry Plantlets

1970 ◽  
Vol 18 (2) ◽  
pp. 187-195 ◽  
Author(s):  
Zhao Guang-jie ◽  
Wang Zhan-bin ◽  
Wang Dan
Keyword(s):  
Tissue Culture ◽  
Key Words ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Root Formation ◽  
Early Stage ◽  
In Vitro Regeneration ◽  
Basal Medium ◽  
Ex Vitro

Effects of different concentrations of 2-ip and IBA in WPM basal medium for Blomidon blueberry in vitro propagation and four different rooting agents at the early stage after transplantation showed that 15 mg/l of 2-ip is the best concentration to induce shoots. For optimum in vitro root formation 10 µM IBA was found to be best and four rooting agents for seedling transplantation according to their effects were No.2>, No.4>, No.3 >, water > and No. 1. Key words: Blomidon, Tissue culture, In vitro regeneration, Rooting agent D.O.I. 10.3329/ptcb.v18i2.3650 Plant Tissue Cult. & Biotech. 18(1): 187-195, 2008 (December)

scholarly journals In vitro Propagation of Bitter Gourd (Momordica charantia L.) from Nodal and Root Segments

1970 ◽  
Vol 19 (1) ◽  
pp. 45-52 ◽  
Author(s):  
M.A.Z. Al Munsur ◽  
M.S. Haque ◽  
K.M. Nasiruddin ◽  
M.S. Hossain
Keyword(s):  
Key Words ◽  
Shoot Regeneration ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Momordica Charantia ◽  
Shoot Length ◽  
Bitter Gourd ◽  
Nodal Segments ◽  
Root Segments

Explants of nodal and root segments of bitter gourd were cultured on MS supplemented with various concentrations of BAP in combination with either 2,4-D or NAA. Nodal segments produced the highest percentage (93.75) of callus in MS supplemented with 1.0 mg/l 2,4-D and 1.0 mg/l BAP whereas, root segments produced the highest (85.00%) callus in 0.6 mg/l NAA and 2.5 mg/l BAP combination. A combination of 1.0 mg/l 2,4-D and 1.0 mg/l BAP exhibited 75.00% shoot regeneration from nodal segments. The highest shoot length (5.15 cm) was recorded with 2.5 mg/l BAP and 0.2 mg/l IAA from nodal segments. No sign of regeneration of shoot was found from root segments in any of the combinations. However, some combinations produced only roots.  Key words: In vitro propagation, bitter gourd, nodal segments, Root segments D.O.I. 10.3329/ptcb.v19i1.4916 Plant Tissue Cult. & Biotech. 19(1): 45-52, 2009 (June)

In vitro assessment of corpus luteum function in cattle following Ovsynch and CIDR ovulation synchronization protocols

2004 ◽  
Vol 84 (4) ◽  
pp. 721-724 ◽  
Author(s):  
M. Aali ◽  
J. A. Small ◽  
G. Giritharan ◽  
N. Ramakrishnappa ◽  
K. M. Cheng ◽  
...  
Keyword(s):  
Key Words ◽  
Corpus Luteum ◽  
Hormone Treatment ◽  
Beef Cows ◽  
In Vitro Production ◽  
Pooled Data ◽  
Progesterone Production ◽  
In Vitro Assessment ◽  
Vitro Production

Non-lactating beef cows (N = 40) were used to determine in vitro production of progesterone by CLs collected on days 6–8, 13–15 and 19–20, following Ovsynch or CIDR ovulation synchronization protocols. Progesterone released by the CL tissues into the medium was measured after 1 h of incubation (control) and after 6 h of hormone treatments (LH, PGF2α or LH + PGF2α). In vitro progesterone production did not differ (P > 0.05) between Ovsynch and CIDR ovulation synchronization protocols. Pooled data, irrespective of ovulation synchronization treatments, showed interaction (P < 0.05) between hormone treatment and stage of CL. Key words: Corpus luteum, progesterone, cows, ovulation synchronization

IN VITRO PRODUCTION OF GROWTH REGULATORS FOR HUMAN EPITHELIAL CELLS

1991 ◽  
pp. 732-734
Author(s):  
Martin Clynes ◽  
Margaret Dooley ◽  
Breda Carey ◽  
Anne Godfrev ◽  
Angela O'Toole ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Growth Regulators ◽  
In Vitro Production ◽  
Human Epithelial Cells ◽  
Vitro Production

scholarly journals In vitro Propagation of Albizia guachapele, Cedrela odorata, Platymiscium pinnatum and Guaiacum sanctum

1970 ◽  
Vol 18 (2) ◽  
pp. 151-156
Author(s):  
L. Valverde-Cerdas ◽  
A. Rojas-Vargas ◽  
A. Hine-Gómez
Keyword(s):  
Costa Rica ◽  
Key Words ◽  
Plant Species ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Cedrela Odorata ◽  
Resistant Material ◽  
In Vitro Micropropagation ◽  
Guaiacum Sanctum

In Costa Rica Albizia guachapele, Cedrela odorata, Platymiscium pinnatum and Guaiacum sanctum are important plant species in both economic and ecological terms and their wood is precious and reported to be highly resistant material. This research has evaluated the in vitro micropropagation as a technology focused to conserve these species. Findings include percentage of germination of seeds and contamination, induction of buds, rooting and growth of micro-cuttings of these four species. Key words:  In vitro, Propagation, Albizia guachapele, Cedrela odorata, Platymiscium pinnatum, Guaiacum sanctum D.O.I. 10.3329/ptcb.v18i2.3397 Plant Tissue Cult. & Biotech. 18(2): 151-156, 2008 (December)

Sign in / Sign up

Export Citation Format

Share Document