scholarly journals Characterization of Immortalized Human Corneal Endothelial Cell Line using HPV 16 E6/E7 on Lyophilized Human Amniotic Membrane

2006 ◽  
Vol 20 (1) ◽  
pp. 47 ◽  
Author(s):  
Hyun-Ju Kim ◽  
Yang-Hwan Ryu ◽  
Jae-Il Ahn ◽  
Jeong-Keuk Park ◽  
Jae-Chan Kim
2004 ◽  
Vol 48 (5) ◽  
pp. 454-459 ◽  
Author(s):  
Jun Seop Shin ◽  
In Keun Jang ◽  
Chan Wha Kim ◽  
Jae Chan Kim

1998 ◽  
Vol 125 (2) ◽  
pp. 357-364 ◽  
Author(s):  
Alan R Conant ◽  
Michael J Fisher ◽  
Alexander G McLennan ◽  
Alec W M Simpson

2012 ◽  
Vol 31 (4) ◽  
pp. 364-371 ◽  
Author(s):  
Tanja Krüger ◽  
Yi Cao ◽  
Søren K. Kjærgaard ◽  
Lisbeth E. Knudsen ◽  
Eva C. Bonefeld-Jørgensen

Phthalates are industrial chemicals used in many cosmetics. We evaluated an in vitro model for eye irritancy testing using the human corneal endothelial cell line B4G12. Cell proliferation and toxicity were assessed after exposing to di- n-butyl phthalate (DBP), benzyl butyl phthalate (BBP), di-2-ethylhexyl phthalate (DEHP), diisodecyl phthalate (DIDP), di- n-octyl phthalate (DnOP), and di-isononyl phthalate (DINP). Gene expression and secretion of inflammatory cytokines were evaluated after exposure to DBP. Decreased cell proliferation was observed for the phthalates DBP, BBP, and DEHP, and cell toxicity was observed for DBP and BBP. Upon DBP exposure at nontoxic concentrations, a significant increased gene expression and cytokine cell secretion were observed for interleukin-1β (IL-1β) and IL-8, and also an increased IL-6 secretion was observed. In conclusion, the human corneal endothelial cell line B4G12 may be a potential model for inflammatory eye irritancy testing of phthalates.


2005 ◽  
Vol 3 (3) ◽  
pp. 105-115 ◽  
Author(s):  
T. Ando ◽  
P. Jordan ◽  
T. Joh ◽  
Y. Wang ◽  
M.H. Jennings ◽  
...  

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