scholarly journals Multiplex PCR for Rapid Detection of Genes Multiplex PCR for Rapid Detection of Genes Encoding Class A Carbapenemases

2012 ◽  
Vol 32 (5) ◽  
pp. 359-361 ◽  
Author(s):  
Sang Sook Hong ◽  
Kyeongmi Kim ◽  
Ji Young Huh ◽  
Bochan Jung ◽  
Myung Seo Kang ◽  
...  
2010 ◽  
Vol 30 (4) ◽  
pp. 1312-1316 ◽  
Author(s):  
Ömer KÜÇÜKBASMACI ◽  
Kenan MİDİLLİ ◽  
Ghassan ISSA ◽  
Özlem GÜVEN ◽  
Nevriye GÖNÜLLÜ

2006 ◽  
Vol 59 (2) ◽  
pp. 321-322 ◽  
Author(s):  
M. J. Ellington ◽  
J. Kistler ◽  
D. M. Livermore ◽  
N. Woodford

2009 ◽  
Vol 58 (11) ◽  
pp. 1522-1524 ◽  
Author(s):  
Anna Karina Mostachio ◽  
Inneke van der Heidjen ◽  
Flavia Rossi ◽  
Anna Sara Levin ◽  
Silvia F. Costa

1999 ◽  
Vol 65 (2) ◽  
pp. 868-872 ◽  
Author(s):  
Peter K. Fagan ◽  
Michael A. Hornitzky ◽  
Karl A. Bettelheim ◽  
Steven P. Djordjevic

ABSTRACT A multiplex PCR was developed for the rapid detection of genes encoding Shiga toxins 1 and 2 (stx1 andstx2 ), intimin (eaeA), and enterohemolysin A (hlyA) in 444 fecal samples derived from healthy and clinically affected cattle, sheep, pigs, and goats. The method involved non-solvent-based extraction of nucleic acid from an aliquot of an overnight culture of feces in EC (modified) broth. The detection limit of the assay for both fecal samples and pure cultures was between 18 and 37 genome equivalents. stx1 and hlyA were the most commonly encountered virulence factors.


2005 ◽  
Vol 57 (1) ◽  
pp. 154-155 ◽  
Author(s):  
Neil Woodford ◽  
Elizabeth J. Fagan ◽  
Matthew J. Ellington

2009 ◽  
Vol 41 (3) ◽  
pp. 181 ◽  
Author(s):  
Junyoung Kim ◽  
Semi Jeon ◽  
Hogeun Rhie ◽  
Bokkwon Lee ◽  
Misun Park ◽  
...  

2004 ◽  
Vol 42 (6) ◽  
pp. 2821-2824 ◽  
Author(s):  
S. Chattopadhyay ◽  
R. Patra ◽  
T. Ramamurthy ◽  
A. Chowdhury ◽  
A. Santra ◽  
...  

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