Performance Assessment of Advansure™ MDR-TB Genoblot Assay Kit for Anti-tuberculosis Drug Susceptibility Test

2012 ◽  
Vol 2 (1) ◽  
pp. 34 ◽  
Author(s):  
Sang Bong Han ◽  
Yongjun Jo ◽  
Jin Kyung Yu ◽  
Yonggoo Kim ◽  
Yeon-Joon Park
Keyword(s):  
Performance Assessment ◽  
Drug Susceptibility ◽  
Susceptibility Test ◽  
Drug Susceptibility Test ◽  
Mdr Tb

scholarly journals Development of Drug Resistance among Comorbid and Non-Comorbid HIV-Negative Relapsed Cases of Tuberculosis in Lahore, Pakistan

2020 ◽  
Author(s):  
Arslan Ahmed Salam ◽  
Aamer Ikram ◽  
Maha Fatima ◽  
Najma Javed Awan
Keyword(s):  
Drug Resistance ◽  
Drug Susceptibility ◽  
Susceptibility Test ◽  
Rifampicin Resistance ◽  
Drug Susceptibility Test ◽  
Health Crisis ◽  
Mdr Tb ◽  
History Of ◽  
Xpert Assay ◽  
Hiv Negative

Abstract Background: Mycobacterium tuberculosis sometimes become resistant to the drugs that are used to treat it. Drug resistant TB (DR-TB) is spread in the same way as drug susceptible TB. DR-TB is a public health crisis. This study aims to find the pattern of drug resistance and correlations between drug resistance and comorbid/non-comorbid conditions in patients with a relapse of TB. Methodology: A cross-sectional study was conducted among 200 HIV-negative relapsed TB patients from 2016-2017 in Mayo Hospital Lahore. The patients’ sputum samples were tested by Ziehl-Neelsen staining to observe acid-fast bacilli. The demographics and medical history of patients was recorded, who were positive for AFB in their sputum samples. Molecular procedure of Gene-Xpert assay was conducted to detect the presence of MTB and rifampicin resistance in the samples. Whereas, the drug susceptibility test (DST) was conducted on the LJ culture medium containing drugs.Results: Out of 200 relapsed TB cases; 97 were comorbid, 99 were non-comorbid. The most prevalent comorbidities were hypertension (42 cases- 43.3%), diabetes (45 cases-46.4%) and hepatitis B (14 cases-14.4%). Among 97 comorbid patients; 37 worked as laborers, 43 earned less than 20,000 PKR and 23 were found to have a history of imprisonment. Whereas in non-comorbid patients; 20 worked as laborers, 28 earned less than 20,000 PKR and 12 had been in prison before. The Gene-Xpert test detected rifampicin resistance (RR) in 20 comorbid (20.6%) and 33 non-comorbid (33.3%) patients. Whereas, the drug susceptibility test (DST) showed that 22 comorbid (22.7%) and 33 non-comorbid (33.3%) patients were RR. A contrast was seen in the results of Gene-Xpert and DST; Gene-Xpert detected 3 cases of RR-negative whereas the same 3 cases were found to be RR-positive on DST. Only 1 case was RR-positive on Gene-Xpert but RR-negative on DST. 17 comorbid patients (17.5%) were diagnosed with MDR-TB and 5 (5.2%) with XDR-TB. Whereas, in non-comorbid patients, there were 26 cases of MDR-TB (26.3%) and 5 cases of XDR-TB (5.1%). There were 2 patients (2.1%) resistant to all drugs.Conclusion: There was a deviation in the results of molecular Gene-Xpert assay compared to the conventional culture methods. Drug resistance was relatively higher in non-comorbid patients than comorbid patients, however, the difference between the two is not very significant.

scholarly journals GENOTYPE AND RESISTANCE PATTERNS OF ANTI TUBERCULOSIS TREATMENT (ATT) IN Mycobacterium tuberculosis ISOLATES FROM TUBERCULOSIS CASES WHICH NOT TAKEN ATT

2016 ◽  
Vol 1 (2) ◽  
pp. 79
Author(s):  
Maria Silvia Merry ◽  
Ning Rintiswati ◽  
Yanri Wijayanti S
Keyword(s):  
Drug Susceptibility ◽  
Susceptibility Test ◽  
Tuberculosis Treatment ◽  
Resistance Pattern ◽  
Drug Susceptibility Test ◽  
Chi Square ◽  
Double Burden ◽  
Cross Sectional ◽  
Resistance Patterns ◽  
Mdr Tb

Background: Tuberculosis (TB) is still a prominent health problem which need to be controlled worldwide. In Indonesia, the incidences of TB cases in 2011 were 450.000 cases with mortality rate 175 person per day. The emergence of mycobacterium’s resistance against Anti Tuberculosis Treatment (ATT) gives a double burden to prevent the disease. This resistance against ATT is caused by several things, one of which is the nature of mycobacterium, mutations and genotype strain variation. Objective: The aim of this study is to get a description of ATT’s resistance pattern, genotype of M. tuberculosis, and determined the correlation between M. tuberculosis’ genotypes and the resistance pattern against ATT. Methods: The research methods were cross-sectional and analytical descriptions. Samples used in this research were clinical isolates, which were taken from patients who hadn’t received ATT therapy before. Patients were recruited from BP4 (Balai Pengobatan Penyakit Paru = Health Center for Lung’s Diseases) at Minggiran and Kotagede area, for the period of June 2010 - December 2010. Drug susceptibility test for ATT were done for Isoniazid, Rifampicin, Streptomycin, and Ethambutol using LJ’s proportion method. Whereas for genotyping, we were using PCR-based Spoligotyping, with Dra and Drb primers. Data processing for genotypes and resistance pattern were in descriptive form, while the analysis of the ATT resistance and genotypes correlation were using chi square. Results: From 33 samples collected and tested for resistancy, 17 samples (51,52%) were sensitive to INH, RIF, STREP, and ETAMB while 16 samples (48,48%) were resistant to one or more ATT. We found 1 isolate (3.03%) was MDR TB. Genotype patterns description are 30% (10 isolates) were Beijing strain and 70% (23 isolates) were Non Beijing with a variety of EAI, LAM, U, Harleem, T, Manu, and Miscellanous. The chi square’s analysis results are p = 0,034 (p < 0,05), ratio prevalence 2,96 (95 CI 0,26 – 0,57). Conclusion: The result from drug susceptibility test for ATT are 48,48%, resistant to one regimen or more ATT, while sensitivity 51,52%. Beijing strains were predominant strain (30%). There were significant correlation between the patterns of resistance against ATT and genotype patterns, Beijing strains tend to be resistant by 2,96 times greater than non-Beijing strains.

Slide drug susceptibility test for the detection of multidrug-resistant tuberculosis in Bangladesh

2013 ◽  
Vol 19 (5) ◽  
pp. 818-824 ◽  
Author(s):  
Rashed Noor ◽  
Akter Hossain ◽  
Saurab Kishore Munshi ◽  
Farjana Rahman ◽  
S.M.Mostofa Kamal
Keyword(s):  
Drug Susceptibility ◽  
Multidrug Resistant ◽  
Susceptibility Test ◽  
Drug Susceptibility Test ◽  
Resistant Tuberculosis ◽  
Multidrug Resistant Tuberculosis

scholarly journals Survival, Chemotherapy and Chemosensitivity Predicted by CTC Cultured In Vitro of SCLC Patients

2021 ◽  
Vol 11 ◽  
Author(s):  
Lixia Ju ◽  
Juan Yang ◽  
Changyun Zhai ◽  
Shuizhen Chai ◽  
Zhiyi Dong ◽  
...  
Keyword(s):  
Objective Response ◽  
Therapy Response ◽  
Drug Susceptibility ◽  
Susceptibility Test ◽  
Therapeutic Regimen ◽  
Second Line ◽  
Drug Susceptibility Test ◽  
Line Therapy ◽  
Potential Marker

PurposeThe prognosis for small cell lung cancer (SCLC) patients receiving later-line treatment is very poor and there is still no standard treatments after the second-line setting. Analyzing the susceptibility of chemotherapeutic drugs with circulating tumor cells (CTCs) cultured in vitro may contribute to optimize the therapeutic regimen. However, so far CTCs have been barely used for studying their chemosensitivity due to the lack of technology to obtain wholly intact and viable CTCs.MethodsBased on a retrospective study of the therapeutic response of 99 patients with unresectable SCLC, the CTC count in 14 SCLC patients was detected before and after chemotherapy to evaluate its role as a potential marker of response. Furthermore, the drug susceptibility of CTCs cultured in vitro obtained from ClearCell FX® System was tested and the therapy response was evaluated.ResultsAll of the 99 patients received the first-line chemotherapy and the objective response rate (ORR) was 74.7%. A total of 36 patients received the second-line therapy and the average duration was 2.6 months, and only 11 cases out of them received the third-line therapy but no one responded. The change of CTC counts was identified to be correlated with therapy response. However all the five SCLC patients who were administered with the drugs according to the drug susceptibility test of CTCs for two cycles underwent progression of disease.ConclusionThe results showed that the responses of chemotherapy are very poor in later lines and the drug susceptibility test using CTCs primary cultured in vitro may not benefit the improvement of therapeutic regimen of SCLC patients.

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