scholarly journals Sequence analysis of the internal transcribed spacer (ITS) region reveals a novel clade of Ichthyophonus sp. from rainbow trout

2010 ◽  
Vol 89 ◽  
pp. 179-183 ◽  
Author(s):  
C Rasmussen ◽  
MK Purcell ◽  
JL Gregg ◽  
SE LaPatra ◽  
JR Winton ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Sequence Analysis ◽  
Internal Transcribed Spacer ◽  
Its Region

scholarly journals WildTermitomycesSpecies Collected from Ondo and Ekiti States Are More Related to African Species as Revealed by ITS Region of rDNA

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Victor Olusegun Oyetayo
Keyword(s):  
Sequence Analysis ◽  
Phylogenetic Tree ◽  
Molecular Identification ◽  
Internal Transcribed Spacer ◽  
Its Region ◽  
Its Sequence ◽  
African Countries ◽  
African Species ◽  
Its Sequence Analysis ◽  
Degree Of Similarity

Molecular identification of eighteenTermitomycesspecies collected from two states, Ondo and Ekiti in Nigeria was carried out using the internal transcribed spacer (ITS) region. The amplicons obtained from rDNA ofTermitomycesspecies were compared with existing sequences in the NCBI GenBank. The results of the ITS sequence analysis discriminated between all theTermitomycesspecies (obtained from Ondo and Ekiti States) andTermitomycessp. sequences obtained from NCBI GenBank. The degree of similarity of T1 to T18 to gene ofTermitomycessp. obtained from NCBI ranges between 82 and 99 percent.Termitomycesspecies from Garbon with ascension number AF321374 was the closest relative of T1 to T18 except T12 that has T. eurhizus and T. striatus as the closet relative. Phylogenetic tree generated with ITS sequences obtained from NCBI GenBank data revealed that T1 to T18 are more related toTermitomycesspecies indigenous to African countries such as Senegal, Congo, and Gabon.

Characterization and phylogenetic diversity of Agrobacterium vitis from Serbia based on sequence analysis of 16S-23S rRNA internal transcribed spacer (ITS) region

2014 ◽  
Vol 140 (4) ◽  
pp. 757-768 ◽  
Author(s):  
Nemanja Kuzmanović ◽  
Milan Ivanović ◽  
Anđelka Prokić ◽  
Katarina Gašić ◽  
Nevena Zlatković ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Diversity ◽  
Its Region ◽  
23S Rrna ◽  
Agrobacterium Vitis

scholarly journals New emerging entomopathogenic fungi isolated from soil in South Sumatra (Indonesia) and their filtrate and conidial insecticidal activity against Spodoptera litura

2020 ◽  
Vol 21 (11) ◽  
Author(s):  
Siti Herlinda ◽  
Riski Anwar Efendi ◽  
Radix Suharjo ◽  
Hasbi Hasbi ◽  
Arum Setiawan ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Culture Filtrate ◽  
Entomopathogenic Fungi ◽  
Insecticidal Activity ◽  
Metarhizium Anisopliae ◽  
Internal Transcribed Spacer ◽  
Spodoptera Litura ◽  
Its Region ◽  
Morphological Characters ◽  
Penicillium Citrinum

Abstract. Herlinda S, Efendi RA, Suharjo R, Hasbi, Setiawan A, Elfita, Verawaty M. 2020. New emerging entomopathogenic fungi isolated from soil in South Sumatra (Indonesia) and their filtrate and conidial insecticidal activity against Spodoptera litura. Biodiversitas 21: 5102-5113. This study was carried out to confirm identity of Beauveria bassiana and Metarhizium anisopliae using sequence analysis of Internal Transcribed Spacer (ITS) region as well as the two unknown isolates of EPF and to investigate their filtrate and conidial insecticidal activity against S. litura larvae. The EPF species were identified based on morphological characters and molecular analysis. Their culture filtrate and conidial bioassays were performed on S. litura larvae. The species found were Penicillium citrinum (BKbTp), Talaromyces diversus (MSwTp1), B. bassiana (BSwTd4), and M. anisopliae (MSwTp3) and have been deposited in the GenBank. All EPF species have high insecticidal activity against S. litura larvae. The highest larvae mortality caused by filtrate was found on P. citrinum (98.67%) and not significantly different from those of T. diversus (96%), but significantly different from B. bassiana (86.67%) and M anisopliae (82.67%). The highest larvae mortality caused by the conidia was also occurred to P. citrinum (98.67%) which was not significantly different from those of T. diversus (97.33%), B. bassiana (86.67%), and M. anisopliae (86.67%). The LT50 caused by T. diversus filtrate was the shortest (6.19 days) and not significantly different from those caused by P. citrinum (6.62 days). The shortest LT50 caused by conidia was found on P. citrinum (5.45 days). Therefore, the conidia and filtrate of P. citrinum, a new emerging entomopathogenic fungus, has the highest insecticidal activity. P. citrinum has the potential to be developed into commercial mycoinsecticides.

Cunninghamella binariae, Mucor ardhlaengiktus, Mucor gigasporus and Umbelopsis changbaiensis, newly recorded species from amphibian feces and soil in Korea

Phytotaxa ◽  
2019 ◽  
Vol 425 (1) ◽  
pp. 19-34
Author(s):  
THUONG T.T. NGUYEN ◽  
KEVIN D. HYDE ◽  
HYANG BURM LEE
Keyword(s):  
Sequence Analysis ◽  
Internal Transcribed Spacer ◽  
Morphological Characteristics ◽  
Its Region ◽  
Soil Samples ◽  
28S Rdna ◽  
Rdna Sequences ◽  
28S Rdna Sequences

Four strains, CNUFC SKT18002, CNUFC FKT18033, CNUFC SKT19001, and CNUFC SKT18228 were isolated during an investigation of fungi belonging to the orders Mucorales and Umbelopsidales from amphibian feces and soil samples in Korea. Based on morphological characteristics and the sequence analysis of the internal transcribed spacer (ITS) region and 28S rDNA sequences, the strains CNUFC SKT18002, CNUFC FKT18033, CNUFC SKT19001, and CNUFC SKT18228 were identified as Cunninghamella binariae, Mucor ardhlaengiktus, Mucor gigasporus, and Umbelopsis changbaiensis, respectively. To the best of our knowledge, the species C. binariae, M. ardhlaengiktus, M. gigasporus, and U. changbaiensis have not been previously reported in Korea.

scholarly journals Neofusicoccum spp. Associated with Stem Canker and Dieback of Blueberry in Chile

Plant Disease ◽  
2009 ◽  
Vol 93 (11) ◽  
pp. 1187-1194 ◽  
Author(s):  
J. G. Espinoza ◽  
E. X. Briceño ◽  
E. R. Chávez ◽  
J. R. Úrbez-Torres ◽  
B. A. Latorre
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Internal Transcribed Spacer ◽  
Its Region ◽  
Stem Canker ◽  
Nucleotide Sequence Analysis ◽  
Susceptible Cultivar ◽  
Koch’S Postulates ◽  
Pathogenicity Tests ◽  
Koch's Postulates

Blueberry (Vaccinium spp.) plantings have significantly increased in Chile during the last decade and, currently, over 10,700 ha are cultivated throughout the country. Among other diseases, stem canker and dieback has been frequently observed in commercial plantations with incidences between 15 and 45%. The aim of this study was to identify and characterize Neofusicoccum spp. causing stem canker and dieback of blueberry in Chile. Three species, N. arbuti, N. australe, and N. parvum, were identified based on colony and conidia morphology, and nucleotide sequence analysis of the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2). These Neofusicoccum spp. were found alone or coexisting with Pestalotiopsis spp., Truncatella spp., or Phomopsis spp. Koch's postulates showed all Neofusicoccum spp. isolated from infected plants to be pathogenic when inoculated on blueberry fruit and twigs using both mycelia and conidia suspension. All blueberry cultivars tested, including, Brigitta, Bluecrop, Brightwell, Duke, Elliott, Misty, and O'Neal, were susceptible to Neofusicoccum spp. infection. Pathogenicity tests showed N. parvum to be the most virulent species and Elliott to be the most susceptible cultivar. This report represents the first description of N. arbuti, N. australe, and N. parvum as canker-causing agents on blueberry in Chile.

Phylogeny and taxonomy of the family Arthrodermataceae (dermatophytes) using sequence analysis of the ribosomal ITS region

1999 ◽  
Vol 37 (2) ◽  
pp. 105-114 ◽  
Author(s):  
Y. GRAser ◽  
M. EL Fari ◽  
R. Vilgalys ◽  
A. F. A. Kuijpers ◽  
G. S. DE Hoog ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Its Region ◽  
The Family

Infundibulicybe rufa sp. nov. (Tricholomataceae), a reddish brown species from southwestern China

Phytotaxa ◽  
2016 ◽  
Vol 266 (2) ◽  
pp. 134 ◽  
Author(s):  
QI ZHAO ◽  
YAN-JIA HAO ◽  
JIAN-KUI LIU ◽  
KEVIN D. HYDE ◽  
YANG-YANG CUI ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Biosphere Reserve ◽  
Its Region ◽  
Southwestern China ◽  
New Taxon ◽  
Line Drawings ◽  
Phylogenetic Placement ◽  
Molecular Phylogenetic ◽  
Pileus Surface

Infundibulicybe rufa sp. nov., is described from Jiuzhaigou Biosphere Reserve, southwestern China. It is characterized by the combination of the following characters: umbilicate to slightly infundibuliform, reddish brown pileus; decurrent, cream lamellae; cylindrical stipe concolorous with the pileus surface. Molecular phylogenetic analyses using the nuclear ribosomal internal transcribed spacer (ITS) region indicates that I. rufa is closely related to I. mediterranea and I. bresadolana. A description, line drawings, phylogenetic placement and comparison with allied taxa for the new taxon are presented.

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