scholarly journals In Situ Assessment of the Brain Microcirculation in Mechanically-Ventilated Rabbits Using Sidestream Dark-Field (SDF) Imaging

2011 ◽  
pp. 75-81 ◽  
Author(s):  
M. ŠITINA ◽  
Z. TUREK ◽  
R. PAŘÍZKOVÁ ◽  
V. ČERNÝ
Keyword(s):  
Dark Field ◽  
Vessel Density ◽  
Flow Index ◽  
Target Tissue ◽  
Cerebral Microcirculation ◽  
Sidestream Dark Field ◽  
Mechanically Ventilated ◽  
Sdf Imaging ◽  
The Brain

Assessment of the cerebral microcirculation by on-line visualization has been impossible for a long time. Sidestream dark-field (SDF) imaging is a relatively new method allowing direct visualization of cerebral surface layer microcirculation using hand-held probe for direct contact with target tissue. The aim of this study was to elucidate the feasibility of studying the cerebral microcirculation in situ by SDF imaging and to assess the basic cerebral microcirculatory parameters in mechanically ventilated rabbits. Images were obtained using SDF imaging from the surface of the brain via craniotomy. Clear high contrast SDF images were successfully obtained. Total small-vessel density was 14.6±1.8 mm/mm2, total all-vessel density was 17.9±1.7 mm/mm2, DeBacker score was 12.0±1.6 mm-1 and microvascular flow index was 3.0±0.0. This method seems to be applicable in animal studies with possibility to use SDF imaging also intraoperatively, providing unique opportunity to study cerebral microcirculation during various experimental and clinical settings.

scholarly journals In situ assessment of the liver microcirculation in mechanically ventilated rats using sidestream dark-field imaging

2009 ◽  
pp. 49-55 ◽  
Author(s):  
V Černý ◽  
Z Turek ◽  
R Pařízková
Keyword(s):  
Animal Studies ◽  
Dark Field ◽  
Target Tissue ◽  
Sidestream Dark Field ◽  
Hepatic Microcirculation ◽  
Midline Laparotomy ◽  
Sdf Imaging ◽  
Liver Microcirculation ◽  
Dark Field Imaging

Assessment of hepatic microcirculation by on-line visualization has been impossible for a long time. Sidestream dark-field (SDF) imaging is a relatively new method allowing direct visualization of both mucosal microcirculation and surface layers microcirculation of solid organs using hand-held probe for direct contact with target tissue. The aim of this study was to evaluate the feasibility of studying the rat hepatic microcirculation in situ by SDF imaging. The liver lobes were left in situ, and images were obtained using SDF imaging on the surface of the liver via upper midline laparotomy. Images were captured intermittently during 10-sec apnoea and recorded. The microvascular parameters were compared with previous validation studies. Clear high contrast SDF images were successfully obtained. Quantitative analysis revealed a mean FSD (functional sinusoidal density) of 402±15 cm/cm2, a sinusoidal diameter of 10.2±0.5 μm and postsinusoidal venular diameter of 33.9±13 μm. SDF imaging is a suitable noninvasive method for accurate quantification of the basic microcirculatory parameters of the liver in situ without a need to exteriorize the liver lobes. This method seems to be applicable in animal studies with possibility to use SDF imaging also intraoperatively, providing unique opportunity to study liver microcirculation during various experimental and clinical settings.

VS03.01: QUANTITATIVE IMAGING OF CHANGE IN MICROCIRCULATION BY SIDESTREAM DARK FIELD MICROSCOPY (SDF) AFTER ESOPHAGECTOMY

2018 ◽  
Vol 31 (Supplement_1) ◽  
pp. 47-48
Author(s):  
Sanne Jansen ◽  
Daniel De Bruin ◽  
Mark I Van Berge Henegouwen ◽  
Ton Van Leeuwen ◽  
Suzanne Gisbertz
Keyword(s):  
Anastomotic Leakage ◽  
Gastric Tube ◽  
Average Velocity ◽  
Venous Return ◽  
Conflicts Of Interest ◽  
Dark Field ◽  
Vessel Density ◽  
Flow Index ◽  
High Morbidity ◽  
Sidestream Dark Field

Abstract Description Anastomotic leakage is one of the most severe complications after esophageal resection with gastric tube reconstruction. Impaired perfusion of the gastric fundus is seen as the main contributing factor for this complication. Transection of the left gastric and gastro-epiploic artery and veins results in compromised perfusion in the fundus area which can result in anastomotic dehiscence (5–20%), relating to high morbidity and mortality (3–4%). The main objective of this observational study is to evaluation gastric tube microcirculation with Sidestream Darkfield Microscopy (SDF). Method This study included 22 patients (October 2015 - June 2016). Intra-operative microscopic images of gastric tube microcirculation were obtained with SDF directly after reconstruction. Using software (AVA2.0), the following parameters were evaluated: average velocity (μm/sec), Microvascular Flow Index (MFI), Total Vessel Density (TVD), Perfusion Vessel Density (PVD), Proportion of Perfused Vessels (PPV) and the De Backer Score (DBS), to assess change in perfusion. Results SDF accurately visualized and evaluated microcirculation in all patients. A SDF-stabilizer was used to create stable images. The average velocity decreased significantly towards the fundus (P = 0.001). Also, MFI, PVD and PPV were significantly lower towards the fundus, compared to the base of the gastric tube (P = 0.0002). No differences in TVD and DBS were observed, which was associated to the observed vessel dilation in the fundus-area. This vessel dilation proposes that compromised venous return may play an important role in the development of necrosis and leakage. Three patients developed anastomotic leakage. Conclusion This is the first study presenting quantitative microcirculation imaging with SDF of the gastric tube. Velocity, MFI, TVD and PPV were accurate parameters to observe change in perfusion after reconstruction. Also, vessel dilation in the fundus suggests a role for venous return in the development of ischemia. Quantitative microcirculation with SDF could allow for intra-operative early risk stratification, and, potentially, can result in a reduction of anastomotic leakage. Disclosure All authors have declared no conflicts of interest.

scholarly journals Characteristics and Clinical Significance of Microcirculation in Populations Residing at Different Altitudes

2020 ◽  
Author(s):  
Siqing Ma ◽  
Zong-Zhao He ◽  
Jun-Ming Luo ◽  
Kang Song ◽  
Jing-Yuan Xu ◽  
...  
Keyword(s):  
Field Method ◽  
Dark Field ◽  
Vessel Density ◽  
Physiological Adaptation ◽  
Flow Index ◽  
Sidestream Dark Field ◽  
Blood Oxygen Saturation ◽  
Cell Parameters ◽  
Sublingual Microcirculation ◽  
Different Altitudes

Abstract Background: This study aimed to evaluate changes in microcirculation, physiological characteristics, and the pathomechanism of and potential treatment alternatives for severe septic shock among healthy individuals residing at different altitudes.Methods: Seventy individuals, 35 from Xining and 35 from Nanjing, were recruited, and their body temperature, pulse, respiration, mean arterial pressure, peripheral blood oxygen saturation, and blood cell parameters were determined. Microcirculation indices of sublingual microcirculation were monitored using the sidestream dark field method.Results: Erythrocyte counts and hemoglobin levels were significantly higher among individuals from Xining than among those from Nanjing (P < 0.05); however, platelet counts were significantly lower among those from Xining (P < 0.05). Microcirculation indices of total vessel density, perfused vessel density, and the proportion of perfused vessels were significantly higher among individuals from Xining than among those from Nanjing; however, the microvascular flow index was lower among those from Xining. Microvascular density was significantly higher among individuals from Xining than among those from Nanjing.Conclusion: Microvascular densit may be a physiological adaptation among populations at moderate-to-high altitudes.

Can sidestream dark field (SDF) imaging identify subtle microvascular changes of the bowel during colorectal surgery?

2018 ◽  
Vol 22 (10) ◽  
pp. 793-800
Author(s):  
A. F. J. de Bruin ◽  
A. L. M. Tavy ◽  
K. van der Sloot ◽  
A. Smits ◽  
C. Ince ◽  
...  
Keyword(s):  
Colorectal Surgery ◽  
Dark Field ◽  
Sidestream Dark Field ◽  
Sdf Imaging ◽  
Microvascular Changes

Intestinal Mucosal and Serosal Microcirculation at the Planned Anastomosis during Abdominal Surgery

2019 ◽  
Vol 60 (5-6) ◽  
pp. 248-256 ◽  
Author(s):  
Arthur L.M. Tavy ◽  
Anton F.J. de Bruin ◽  
Anke B. Smits ◽  
E. Christiaan Boerma ◽  
Can Ince ◽  
...  
Keyword(s):  
Anastomotic Leakage ◽  
Dark Field ◽  
Vessel Density ◽  
Flow Index ◽  
Dark Field Imaging ◽  
Key Factor ◽  
Observational Cohort ◽  
Incident Dark Field Imaging ◽  
Field Imaging ◽  
Total Vessel Density

Introduction: Intestinal blood flow is often named as a key factor in the pathophysiology of anastomotic leakage. The distribution between mucosal and serosal microperfusion during surgery remains to be elucidated. Objective: The aim of this study was to assess if the mucosal microcirculation of the intestine is more vulnerable to a surgical hit than the serosal microcirculation during surgery. Methods: In an observational cohort study (n = 9 patients), the microcirculation of the bowel serosa and mucosa was visualized with incident dark-field imaging during surgery. At the planned anastomosis, the following microcirculatory parameters were determined: microvascular flow index (MFI), percentage of perfused vessels (PPV), perfused vessel density (PVD), and total vessel density (TVD). Data are presented as median (interquartile range [IQR]). Results: Perfusion parameters and vessel density were significantly higher for the mucosa than the serosal microcirculation at the planned site for anastomosis or stoma. Mucosal MFI was 3.00 (IQR 3.00–3.00) compared to a serosal MFI of 2.75 (IQR 2.21–2.94), p = 0.03. The PPV was 99% (IQR 98–100) versus 92% (IQR 66–94), p = 0.01. The TVD was 16.77 mm/mm2 (IQR 13.04–18.01) versus 10.42 mm/mm2 (IQR 9.36–11.81), p = 0.01, and the PVD was 15.44 mm/mm2 (IQR 13.04–17.78) versus 9.02 mm/mm2 (IQR 6.43–9.43), p = 0.01. Conclusions: The mucosal microcirculation was preserved, while lower perfusion of the serosa was found at the planned anastomosis or stoma during surgery. Further research is needed to link our observations to the clinically relevant endpoint of anastomotic leakage.

scholarly journals Exposure to acute normobaric hypoxia results in adaptions of both the macro- and microcirculatory system

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Moritz Mirna ◽  
Nana-Yaw Bimpong-Buta ◽  
Fabian Hoffmann ◽  
Thaer Abusamrah ◽  
Thorben Knost ◽  
...  
Keyword(s):  
Cardiac Output ◽  
Acute Hypoxia ◽  
Capillary Density ◽  
Dark Field ◽  
Normobaric Hypoxia ◽  
Vessel Density ◽  
Resistance Arteries ◽  
Compensatory Mechanisms ◽  
Sidestream Dark Field ◽  
Sublingual Microcirculation

AbstractAlthough acute hypoxia is of utmost pathophysiologic relevance in health and disease, studies on its effects on both the macro- and microcirculation are scarce. Herein, we provide a comprehensive analysis of the effects of acute normobaric hypoxia on human macro- and microcirculation. 20 healthy participants were enrolled in this study. Hypoxia was induced in a normobaric hypoxia chamber by decreasing the partial pressure of oxygen in inhaled air stepwisely (pO2; 21.25 kPa (0 k), 16.42 kPa (2 k), 12.63 kPa (4 k) and 9.64 kPa (6 k)). Macrocirculatory effects were assessed by cardiac output measurements, microcirculatory changes were investigated by sidestream dark-field imaging in the sublingual capillary bed and videocapillaroscopy at the nailfold. Exposure to hypoxia resulted in a decrease of systemic vascular resistance (p < 0.0001) and diastolic blood pressure (p = 0.014). Concomitantly, we observed an increase in heart rate (p < 0.0001) and an increase of cardiac output (p < 0.0001). In the sublingual microcirculation, exposure to hypoxia resulted in an increase of total vessel density, proportion of perfused vessels and perfused vessel density. Furthermore, we observed an increase in peripheral capillary density. Exposure to acute hypoxia results in vasodilatation of resistance arteries, as well as recruitment of microvessels of the central and peripheral microcirculation. The observed macro- and microcirculatory effects are most likely a result from compensatory mechanisms to ensure adequate tissue oxygenation.

scholarly journals Alterations of cerebral microcirculation in peritumoral edema: feasibility of in vivo sidestream dark-field imaging in intracranial meningiomas

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Moncef Berhouma ◽  
Thiebaud Picart ◽  
Chloe Dumot ◽  
Isabelle Pelissou-Guyotat ◽  
David Meyronet ◽  
...  
Keyword(s):  
Mass Effect ◽  
Dark Field ◽  
Sidestream Dark Field ◽  
Reference Pattern ◽  
Who Grade ◽  
Perioperative Morbidity ◽  
Sdf Imaging ◽  
Intracranial Meningiomas ◽  
Normal Reference

Abstract Background Intracranial meningiomas display a variable amount of peritumoral brain edema (PTBE), which can significantly impact perioperative morbidity. The role of microcirculatory disturbances in the pathogenesis of PTBE is still debated. The aim of this study was to microscopically demonstrate and intraoperatively quantify, for the first time, the alterations to microcirculation in PTBE using sidestream dark-field (SDF) imaging. Methods Adult patients with WHO grade I meningiomas were recruited over a 9-month period and divided into 2 groups depending on the absence (NE group) or the presence (E group) of PTBE. In vivo intraoperative microcirculation imaging was performed in the peritumoral area before and after microsurgical resection. Results Six patients were included in the NE group and 6 in the E group. At the baseline in the NE group, there was a minor decrease in microcirculatory parameters compared to normal reference values, which was probably due to the mass effect. In contrast, microcirculatory parameters in the E group were significantly altered, affecting both vessel density and blood flow values, with a drop of approximately 50% of normal values. Surgical resection resulted in a quasi-normalization of microcirculation parameters in the NE group, whereas in the E group, even if all parameters statistically significantly improved, post-resection values remained considerably inferior to those of the normal reference pattern. Conclusion Our study confirmed significant alterations of microcirculatory parameters in PTBE in meningiomas. Further in vivo SDF imaging studies may explore the possible correlation between the severity of these microcirculatory alterations and the postoperative neurological outcome.

scholarly journals Sidestream Dark Field (SDF) imaging of oral microcirculation in the assessment of systemic sclerosis

2019 ◽  
Vol 126 ◽  
pp. 103890
Author(s):  
M. Sha ◽  
M. Griffin ◽  
C.P. Denton ◽  
P.E. Butler
Keyword(s):  
Systemic Sclerosis ◽  
Dark Field ◽  
Sidestream Dark Field ◽  
Sdf Imaging
Sign in / Sign up

Export Citation Format

Share Document