The present study investigates possible roles of manure-derived biochar (MBC) in anaerobic digestion (AD) of dairy manure. Addition of MBC led to an increase in cumulative methane yield and a decrease in lag phase under all tested conditions (concentration of MBC: 1 and 10 g/L, temperature: 20, 35 and 55°C). For example, the cumulative methane yield in the mesophilic AD with 10 g/L MBC were 24.51% higher than that of the AD without MBC. Additionally, lag phage of mesophilic AD with 10 g/L MBC decreased from 2.08 d to 1.52 d. Microbial community analysis indicated that the addition of MBC to mesophilic and thermophilic AD of dairy manure increased the relative abundance of <i>Ruminofilibacter</i> which related to the hydrolysis. In addition, the addition of MBC to AD potentially stimulated the growth of syntrophic bacteria (e.g., genera Clostridium, Syntrophomonas and Syntrophus) and hydrogenotrophic methanogens (e.g., genera <i>Methanobacterium, Methanolinea</i> and <i>Methanomassiliicoccus</i>). Furthermore, microbial community analysis also suggested that mediate interspecies electron transfer and direct interspecies electron transfer would be accelerated by addition of MBC which showed high electrical conductivity (3230 μS/cm).
Implementing pressurized reactors and volatile fatty acids alleviated technologies for improved anaerobic digestion performance: Process evaluation and microbial community analysis
